FIONA1-mediated methylation of the 3'UTR of FLC affects FLC transcript levels and flowering in Arabidopsis
Adenosine bases of RNA can be transiently modified by the deposition of a methyl-group to form N6-methyladenosine (m6A). This adenosine-methylation is an ancient process and the enzymes involved are evolutionary highly conserved. A genetic screen designed to identify suppressors of late flowering tr...
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| Veröffentlicht in: | PLoS genetics 2022-09, Vol.18 (9), p.e1010386 |
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| creator | Sun, Bin Bhati, Kaushal Kumar Song, Peizhe Edwards, Ashleigh Petri, Louise Kruusvee, Valdeko Blaakmeer, Anko Dolde, Ulla Rodrigues, Vandasue Straub, Daniel Yang, Junbo Jia, Guifang Wenkel, Stephan |
| description | Adenosine bases of RNA can be transiently modified by the deposition of a methyl-group to form N6-methyladenosine (m6A). This adenosine-methylation is an ancient process and the enzymes involved are evolutionary highly conserved. A genetic screen designed to identify suppressors of late flowering transgenic Arabidopsis plants overexpressing the miP1a microProtein yielded a new allele of the FIONA1 (FIO1) m6A-methyltransferase. To characterize the early flowering phenotype of fio1 mutant plants we employed an integrative approach of mRNA-seq, Nanopore direct RNA-sequencing and meRIP-seq to identify differentially expressed transcripts as well as differentially methylated RNAs. We provide evidence that FIO1 is the elusive methyltransferase responsible for the 3'-end methylation of the FLOWERING LOCUS C (FLC) transcript. Furthermore, our genetic and biochemical data suggest that 3'-methylation stabilizes FLC mRNAs and non-methylated FLC is a target for rapid degradation. |
| doi_str_mv | 10.1371/journal.pgen.1010386 |
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This adenosine-methylation is an ancient process and the enzymes involved are evolutionary highly conserved. A genetic screen designed to identify suppressors of late flowering transgenic Arabidopsis plants overexpressing the miP1a microProtein yielded a new allele of the FIONA1 (FIO1) m6A-methyltransferase. To characterize the early flowering phenotype of fio1 mutant plants we employed an integrative approach of mRNA-seq, Nanopore direct RNA-sequencing and meRIP-seq to identify differentially expressed transcripts as well as differentially methylated RNAs. We provide evidence that FIO1 is the elusive methyltransferase responsible for the 3'-end methylation of the FLOWERING LOCUS C (FLC) transcript. Furthermore, our genetic and biochemical data suggest that 3'-methylation stabilizes FLC mRNAs and non-methylated FLC is a target for rapid degradation.</description><identifier>ISSN: 1553-7404</identifier><identifier>ISSN: 1553-7390</identifier><identifier>EISSN: 1553-7404</identifier><identifier>DOI: 10.1371/journal.pgen.1010386</identifier><identifier>PMID: 36166469</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>3' Untranslated regions ; 3' Untranslated Regions - genetics ; Adenosine - genetics ; Adenosine - metabolism ; Amino acids ; Analysis ; Arabidopsis ; Arabidopsis - metabolism ; Arabidopsis Proteins - metabolism ; Arabidopsis thaliana ; biologi ; biology ; Biology and Life Sciences ; CRISPR ; Flowering locus C ; Flowers - genetics ; Flowers - metabolism ; Gene expression ; Gene Expression Regulation, Plant ; Genetic aspects ; Genetic screening ; Genomes ; Genotype & phenotype ; Growth ; Histones - genetics ; Life Sciences ; MADS Domain Proteins - genetics ; MADS Domain Proteins - metabolism ; Methods ; Methylation ; Methyltransferase ; Methyltransferases - genetics ; Mutation ; N6-methyladenosine ; Phenotypes ; Physical Sciences ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - metabolism ; Proteins ; Research and analysis methods ; RNA sequencing ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Transgenic plants</subject><ispartof>PLoS genetics, 2022-09, Vol.18 (9), p.e1010386</ispartof><rights>COPYRIGHT 2022 Public Library of Science</rights><rights>2022 Sun et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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genetics</subject><subject>Life Sciences</subject><subject>MADS Domain Proteins - genetics</subject><subject>MADS Domain Proteins - metabolism</subject><subject>Methods</subject><subject>Methylation</subject><subject>Methyltransferase</subject><subject>Methyltransferases - genetics</subject><subject>Mutation</subject><subject>N6-methyladenosine</subject><subject>Phenotypes</subject><subject>Physical Sciences</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>Proteins</subject><subject>Research and analysis methods</subject><subject>RNA sequencing</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Transgenic plants</subject><issn>1553-7404</issn><issn>1553-7390</issn><issn>1553-7404</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>D8T</sourceid><sourceid>DOA</sourceid><recordid>eNqVk11v0zAUhiMEYmPwDxBEQgJ20WLHH0lukKrBWKVqk8a2W8uxT1pXbtzFzsb-PU7bTcu0C1Au_PW8r09e6yTJe4zGmOT429J1bSPteD2HZowRRqTgL5J9zBgZ5RTRl4_me8kb75cIEVaU-etkj3DMOeXlfrI8np6dTvBoBdrIADpdQVjcWRmMa1JXp2EBKflyeXHeL45nR6msa1DBb-ahlY1XrVmH1MINWJ_KRqe1dbfQmmaemiadtLIy2q298W-TV7W0Ht7txoPk8vjnxdHJaHb2a3o0mY1UXhZhVPGCoUJJqUqmM1RyznJNNJeqooQqTTXBJdcVZ_Go4hJLVlVFXVSlIhWL_3iQfNz6rq3zYheTF1mesSynBOFITLeEdnIp1q1ZyfZOOGnEZsO1cyHbYJQFUbIqU5JDoSWmUJIC4mUlkzyuNeS912jr5W9h3VUDtx_marJx61adyBBHJY389111XRVDV9DEFO1ANjxpzELM3U2shJKSZdHgcGuweCI7mcxEv4donpEiozd9cV93l7XuugMfxMp4BdbKBlzXZ4ILTihGPKKfnqDPJ7ej5jKGY5raxRpVbyomeYZYzkjee42foeKnYWWUa6A2cX8gOBwIIhPgT5jLznsx_X3-H-zpv7NnV0P28yN2AdKGhXe26zvBD0G6BVXrvG-hfngFjETfmvfJib41xa41o-zD44d_EN33IvkLhokwtg</recordid><startdate>20220927</startdate><enddate>20220927</enddate><creator>Sun, Bin</creator><creator>Bhati, Kaushal Kumar</creator><creator>Song, Peizhe</creator><creator>Edwards, Ashleigh</creator><creator>Petri, Louise</creator><creator>Kruusvee, Valdeko</creator><creator>Blaakmeer, Anko</creator><creator>Dolde, Ulla</creator><creator>Rodrigues, Vandasue</creator><creator>Straub, Daniel</creator><creator>Yang, Junbo</creator><creator>Jia, Guifang</creator><creator>Wenkel, Stephan</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISN</scope><scope>ISR</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><scope>ADHXS</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D8T</scope><scope>D93</scope><scope>ZZAVC</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-1201-762X</orcidid><orcidid>https://orcid.org/0000-0002-7007-4027</orcidid><orcidid>https://orcid.org/0000-0002-2322-0431</orcidid><orcidid>https://orcid.org/0000-0001-5764-9423</orcidid><orcidid>https://orcid.org/0000-0002-1828-6284</orcidid><orcidid>https://orcid.org/0000-0003-2187-5749</orcidid><orcidid>https://orcid.org/0000-0002-9553-400X</orcidid><orcidid>https://orcid.org/0000-0002-3471-3944</orcidid><orcidid>https://orcid.org/0000-0002-1834-2573</orcidid><orcidid>https://orcid.org/0000-0002-2553-0660</orcidid><orcidid>https://orcid.org/0000-0002-1455-7171</orcidid></search><sort><creationdate>20220927</creationdate><title>FIONA1-mediated methylation of the 3'UTR of FLC affects FLC transcript levels and flowering in Arabidopsis</title><author>Sun, Bin ; Bhati, Kaushal Kumar ; Song, Peizhe ; Edwards, Ashleigh ; Petri, Louise ; Kruusvee, Valdeko ; Blaakmeer, Anko ; Dolde, Ulla ; Rodrigues, Vandasue ; Straub, Daniel ; Yang, Junbo ; Jia, Guifang ; Wenkel, Stephan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c798t-b68508caac95d2096657d3d6acb434cd4d3196db65966b6a1a5bb8f8b9c3b5003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>3' Untranslated regions</topic><topic>3' Untranslated Regions - 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| recordid | cdi_plos_journals_2725274301 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; SWEPUB Freely available online; Public Library of Science (PLoS) |
| subjects | 3' Untranslated regions 3' Untranslated Regions - genetics Adenosine - genetics Adenosine - metabolism Amino acids Analysis Arabidopsis Arabidopsis - metabolism Arabidopsis Proteins - metabolism Arabidopsis thaliana biologi biology Biology and Life Sciences CRISPR Flowering locus C Flowers - genetics Flowers - metabolism Gene expression Gene Expression Regulation, Plant Genetic aspects Genetic screening Genomes Genotype & phenotype Growth Histones - genetics Life Sciences MADS Domain Proteins - genetics MADS Domain Proteins - metabolism Methods Methylation Methyltransferase Methyltransferases - genetics Mutation N6-methyladenosine Phenotypes Physical Sciences Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism Proteins Research and analysis methods RNA sequencing RNA, Messenger - genetics RNA, Messenger - metabolism Transgenic plants |
| title | FIONA1-mediated methylation of the 3'UTR of FLC affects FLC transcript levels and flowering in Arabidopsis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-12T19%3A49%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=FIONA1-mediated%20methylation%20of%20the%203'UTR%20of%20FLC%20affects%20FLC%20transcript%20levels%20and%20flowering%20in%20Arabidopsis&rft.jtitle=PLoS%20genetics&rft.au=Sun,%20Bin&rft.date=2022-09-27&rft.volume=18&rft.issue=9&rft.spage=e1010386&rft.pages=e1010386-&rft.issn=1553-7404&rft.eissn=1553-7404&rft_id=info:doi/10.1371/journal.pgen.1010386&rft_dat=%3Cgale_plos_%3EA720575376%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2725274301&rft_id=info:pmid/36166469&rft_galeid=A720575376&rft_doaj_id=oai_doaj_org_article_95b2ca6e8da14e938ebb895a6da1de71&rfr_iscdi=true |