OverFlap PCR: A reliable approach for generating plasmid DNA libraries containing random sequences without a template bias

Over the decades, practical biotechnology researchers have aimed to improve naturally occurring proteins and create novel ones. It is widely recognized that coupling protein sequence randomization with various effect screening methodologies is one of the most powerful techniques for quickly, efficie...

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Veröffentlicht in:	PloS one 2022-08, Vol.17 (8), p.e0262968-e0262968
Hauptverfasser:	Linars, Artis, Silamikelis, Ivars, Gudra, Dita, Roga, Ance, Fridmanis, Davids
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acid sequence Bias Biology and Life Sciences Biotechnology Deoxyribonucleic acid DNA Engineering and Technology Gene amplification Gene sequencing Genetic research Methods Mutagenesis Nucleotide sequence Peptides Plasmids Polymerase chain reaction Proteins Research and Analysis Methods Success
Online-Zugang:	Volltext
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