Biodegradable microneedle patch for delivery of meloxicam for managing pain in cattle
Microneedle patches are a promising source for transdermal diffusion of macromolecules and are designed to painlessly penetrate the skin. In this study, a biodegradable chitosan microneedle patch to deliver meloxicam for managing pain in cattle was tested. The potential of reuse of the polymeric sol...
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| Veröffentlicht in: | PloS one 2022-08, Vol.17 (8), p.e0272169-e0272169 |
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| creator | Castilla-Casadiego, David A Miranda-MunÌoz, Katherine A Roberts, Jesse L Crowell, Anne D Gonzalez-Nino, David Choudhury, Dipankar Aparicio-Solis, Frank O Servoss, Shannon L Rosales, Adrianne M Prinz, Gary Zou, Min Zhang, Yuntao Coetzee, Johann F Greenlee, Lauren F Powell, Jeremy Almodovar, Jorge |
| description | Microneedle patches are a promising source for transdermal diffusion of macromolecules and are designed to painlessly penetrate the skin. In this study, a biodegradable chitosan microneedle patch to deliver meloxicam for managing pain in cattle was tested. The potential of reuse of the polymeric solution to fabricate the patches, optimization of fabrication, morphological analysis of the microneedle patch and analysis of preservation of the chemical composition after sterilization were evaluated. In-vitro analysis consisted of studying in-vitro penetration mechanical properties, compression testing analysis of microneedle patch, and in-vitro drug release analysis. In-vivo studies were performed to analyze the dissolution capability of the microneedle patch. Results regarding the physical characteristics, chemical composition, and mechanical properties confirmed that rheological properties of the chitosan solution, present significant differences over time, demonstrating that reusing the solution on the fourth day results in failure patches. Morphological characteristics and chemical composition studies revealed that the process of sterilization (ethylene oxide gas) needed for implanting the patches into the skin did not affect the properties of microneedle patches. In-vitro studies showed that approximately 33.02 ± 3.88% of the meloxicam was released over 7 days. A full penetration of the microneedles into the skin can be obtained by applying approximately 3.2 N. In-vivo studies demonstrated that microneedle patches were capable of swelling and dissolving, exhibiting a dissolution percentage of more than 50% of the original height of microneedle after 7 days. No abnormal tissue, swelling, or inflammation was observed in the implanted area. The results of this work show that chitosan biodegradable microneedle patches may be useful to deliver meloxicam to improve pain management of cattle with positive effects for commercial manufacturing. |
| doi_str_mv | 10.1371/journal.pone.0272169 |
| format | Article |
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In this study, a biodegradable chitosan microneedle patch to deliver meloxicam for managing pain in cattle was tested. The potential of reuse of the polymeric solution to fabricate the patches, optimization of fabrication, morphological analysis of the microneedle patch and analysis of preservation of the chemical composition after sterilization were evaluated. In-vitro analysis consisted of studying in-vitro penetration mechanical properties, compression testing analysis of microneedle patch, and in-vitro drug release analysis. In-vivo studies were performed to analyze the dissolution capability of the microneedle patch. Results regarding the physical characteristics, chemical composition, and mechanical properties confirmed that rheological properties of the chitosan solution, present significant differences over time, demonstrating that reusing the solution on the fourth day results in failure patches. Morphological characteristics and chemical composition studies revealed that the process of sterilization (ethylene oxide gas) needed for implanting the patches into the skin did not affect the properties of microneedle patches. In-vitro studies showed that approximately 33.02 ± 3.88% of the meloxicam was released over 7 days. A full penetration of the microneedles into the skin can be obtained by applying approximately 3.2 N. In-vivo studies demonstrated that microneedle patches were capable of swelling and dissolving, exhibiting a dissolution percentage of more than 50% of the original height of microneedle after 7 days. No abnormal tissue, swelling, or inflammation was observed in the implanted area. The results of this work show that chitosan biodegradable microneedle patches may be useful to deliver meloxicam to improve pain management of cattle with positive effects for commercial manufacturing.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0272169</identifier><identifier>PMID: 35917312</identifier><language>eng</language><publisher>San Francisco: Public Library of Science</publisher><subject>Animals ; Biodegradability ; Biodegradation ; Biology and Life Sciences ; Cadavers ; Care and treatment ; Cattle ; Chemical composition ; Chitosan ; Compression ; Dissolution ; Drug delivery systems ; Engineering and Technology ; Ethylene oxide ; Fabrication ; Health aspects ; Macromolecules ; Mechanical properties ; Medicine and Health Sciences ; Meloxicam ; Molecular weight ; Morphology ; Needles ; Nonsteroidal anti-inflammatory drugs ; Optimization ; Pain ; Pain management ; Patches (structures) ; Penetration ; Physical characteristics ; Physical properties ; Physical Sciences ; Research and Analysis Methods ; Reuse ; Rheological properties ; Skin ; Sterilization ; Swelling ; Transdermal medication</subject><ispartof>PloS one, 2022-08, Vol.17 (8), p.e0272169-e0272169</ispartof><rights>COPYRIGHT 2022 Public Library of Science</rights><rights>2022 Castilla-Casadiego et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 Castilla-Casadiego et al 2022 Castilla-Casadiego et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c669t-40bbff77042d6da03596455c602d3e367727f8ca24e1efd516bab564af681fc93</citedby><cites>FETCH-LOGICAL-c669t-40bbff77042d6da03596455c602d3e367727f8ca24e1efd516bab564af681fc93</cites><orcidid>0000-0003-3075-5423 ; 0000-0002-7035-1100 ; 0000-0002-1151-3878 ; 0000-0003-2579-3214 ; 0000-0001-5787-6888</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345335/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345335/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23871,27929,27930,53796,53798</link.rule.ids></links><search><contributor>Santos, Hélder A.</contributor><creatorcontrib>Castilla-Casadiego, David A</creatorcontrib><creatorcontrib>Miranda-MunÌoz, Katherine A</creatorcontrib><creatorcontrib>Roberts, Jesse L</creatorcontrib><creatorcontrib>Crowell, Anne D</creatorcontrib><creatorcontrib>Gonzalez-Nino, David</creatorcontrib><creatorcontrib>Choudhury, Dipankar</creatorcontrib><creatorcontrib>Aparicio-Solis, Frank O</creatorcontrib><creatorcontrib>Servoss, Shannon L</creatorcontrib><creatorcontrib>Rosales, Adrianne M</creatorcontrib><creatorcontrib>Prinz, Gary</creatorcontrib><creatorcontrib>Zou, Min</creatorcontrib><creatorcontrib>Zhang, Yuntao</creatorcontrib><creatorcontrib>Coetzee, Johann F</creatorcontrib><creatorcontrib>Greenlee, Lauren F</creatorcontrib><creatorcontrib>Powell, Jeremy</creatorcontrib><creatorcontrib>Almodovar, Jorge</creatorcontrib><title>Biodegradable microneedle patch for delivery of meloxicam for managing pain in cattle</title><title>PloS one</title><description>Microneedle patches are a promising source for transdermal diffusion of macromolecules and are designed to painlessly penetrate the skin. In this study, a biodegradable chitosan microneedle patch to deliver meloxicam for managing pain in cattle was tested. The potential of reuse of the polymeric solution to fabricate the patches, optimization of fabrication, morphological analysis of the microneedle patch and analysis of preservation of the chemical composition after sterilization were evaluated. In-vitro analysis consisted of studying in-vitro penetration mechanical properties, compression testing analysis of microneedle patch, and in-vitro drug release analysis. In-vivo studies were performed to analyze the dissolution capability of the microneedle patch. Results regarding the physical characteristics, chemical composition, and mechanical properties confirmed that rheological properties of the chitosan solution, present significant differences over time, demonstrating that reusing the solution on the fourth day results in failure patches. Morphological characteristics and chemical composition studies revealed that the process of sterilization (ethylene oxide gas) needed for implanting the patches into the skin did not affect the properties of microneedle patches. In-vitro studies showed that approximately 33.02 ± 3.88% of the meloxicam was released over 7 days. A full penetration of the microneedles into the skin can be obtained by applying approximately 3.2 N. In-vivo studies demonstrated that microneedle patches were capable of swelling and dissolving, exhibiting a dissolution percentage of more than 50% of the original height of microneedle after 7 days. No abnormal tissue, swelling, or inflammation was observed in the implanted area. The results of this work show that chitosan biodegradable microneedle patches may be useful to deliver meloxicam to improve pain management of cattle with positive effects for commercial manufacturing.</description><subject>Animals</subject><subject>Biodegradability</subject><subject>Biodegradation</subject><subject>Biology and Life Sciences</subject><subject>Cadavers</subject><subject>Care and treatment</subject><subject>Cattle</subject><subject>Chemical composition</subject><subject>Chitosan</subject><subject>Compression</subject><subject>Dissolution</subject><subject>Drug delivery systems</subject><subject>Engineering and Technology</subject><subject>Ethylene oxide</subject><subject>Fabrication</subject><subject>Health aspects</subject><subject>Macromolecules</subject><subject>Mechanical properties</subject><subject>Medicine and Health Sciences</subject><subject>Meloxicam</subject><subject>Molecular weight</subject><subject>Morphology</subject><subject>Needles</subject><subject>Nonsteroidal anti-inflammatory drugs</subject><subject>Optimization</subject><subject>Pain</subject><subject>Pain management</subject><subject>Patches (structures)</subject><subject>Penetration</subject><subject>Physical characteristics</subject><subject>Physical properties</subject><subject>Physical Sciences</subject><subject>Research and Analysis Methods</subject><subject>Reuse</subject><subject>Rheological properties</subject><subject>Skin</subject><subject>Sterilization</subject><subject>Swelling</subject><subject>Transdermal 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microneedle patch for delivery of meloxicam for managing pain in cattle</title><author>Castilla-Casadiego, David A ; Miranda-MunÌoz, Katherine A ; Roberts, Jesse L ; Crowell, Anne D ; Gonzalez-Nino, David ; Choudhury, Dipankar ; Aparicio-Solis, Frank O ; Servoss, Shannon L ; Rosales, Adrianne M ; Prinz, Gary ; Zou, Min ; Zhang, Yuntao ; Coetzee, Johann F ; Greenlee, Lauren F ; Powell, Jeremy ; Almodovar, Jorge</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c669t-40bbff77042d6da03596455c602d3e367727f8ca24e1efd516bab564af681fc93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animals</topic><topic>Biodegradability</topic><topic>Biodegradation</topic><topic>Biology and Life Sciences</topic><topic>Cadavers</topic><topic>Care and treatment</topic><topic>Cattle</topic><topic>Chemical 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A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biodegradable microneedle patch for delivery of meloxicam for managing pain in cattle</atitle><jtitle>PloS one</jtitle><date>2022-08-02</date><risdate>2022</risdate><volume>17</volume><issue>8</issue><spage>e0272169</spage><epage>e0272169</epage><pages>e0272169-e0272169</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Microneedle patches are a promising source for transdermal diffusion of macromolecules and are designed to painlessly penetrate the skin. In this study, a biodegradable chitosan microneedle patch to deliver meloxicam for managing pain in cattle was tested. The potential of reuse of the polymeric solution to fabricate the patches, optimization of fabrication, morphological analysis of the microneedle patch and analysis of preservation of the chemical composition after sterilization were evaluated. In-vitro analysis consisted of studying in-vitro penetration mechanical properties, compression testing analysis of microneedle patch, and in-vitro drug release analysis. In-vivo studies were performed to analyze the dissolution capability of the microneedle patch. Results regarding the physical characteristics, chemical composition, and mechanical properties confirmed that rheological properties of the chitosan solution, present significant differences over time, demonstrating that reusing the solution on the fourth day results in failure patches. Morphological characteristics and chemical composition studies revealed that the process of sterilization (ethylene oxide gas) needed for implanting the patches into the skin did not affect the properties of microneedle patches. In-vitro studies showed that approximately 33.02 ± 3.88% of the meloxicam was released over 7 days. A full penetration of the microneedles into the skin can be obtained by applying approximately 3.2 N. In-vivo studies demonstrated that microneedle patches were capable of swelling and dissolving, exhibiting a dissolution percentage of more than 50% of the original height of microneedle after 7 days. No abnormal tissue, swelling, or inflammation was observed in the implanted area. The results of this work show that chitosan biodegradable microneedle patches may be useful to deliver meloxicam to improve pain management of cattle with positive effects for commercial manufacturing.</abstract><cop>San Francisco</cop><pub>Public Library of Science</pub><pmid>35917312</pmid><doi>10.1371/journal.pone.0272169</doi><tpages>e0272169</tpages><orcidid>https://orcid.org/0000-0003-3075-5423</orcidid><orcidid>https://orcid.org/0000-0002-7035-1100</orcidid><orcidid>https://orcid.org/0000-0002-1151-3878</orcidid><orcidid>https://orcid.org/0000-0003-2579-3214</orcidid><orcidid>https://orcid.org/0000-0001-5787-6888</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2022-08, Vol.17 (8), p.e0272169-e0272169 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2697325055 |
| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS) Journals Open Access; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animals Biodegradability Biodegradation Biology and Life Sciences Cadavers Care and treatment Cattle Chemical composition Chitosan Compression Dissolution Drug delivery systems Engineering and Technology Ethylene oxide Fabrication Health aspects Macromolecules Mechanical properties Medicine and Health Sciences Meloxicam Molecular weight Morphology Needles Nonsteroidal anti-inflammatory drugs Optimization Pain Pain management Patches (structures) Penetration Physical characteristics Physical properties Physical Sciences Research and Analysis Methods Reuse Rheological properties Skin Sterilization Swelling Transdermal medication |
| title | Biodegradable microneedle patch for delivery of meloxicam for managing pain in cattle |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-12T01%3A26%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Biodegradable%20microneedle%20patch%20for%20delivery%20of%20meloxicam%20for%20managing%20pain%20in%20cattle&rft.jtitle=PloS%20one&rft.au=Castilla-Casadiego,%20David%20A&rft.date=2022-08-02&rft.volume=17&rft.issue=8&rft.spage=e0272169&rft.epage=e0272169&rft.pages=e0272169-e0272169&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0272169&rft_dat=%3Cgale_plos_%3EA712277927%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2697325055&rft_id=info:pmid/35917312&rft_galeid=A712277927&rft_doaj_id=oai_doaj_org_article_cbb414d6ce4c4e2981b19efdddd25cef&rfr_iscdi=true |