Surface pre-reacted glass-ionomer eluate protects gingival epithelium from penetration by lipopolysaccharides and peptidoglycans via transcription factor EB pathway
Surface pre-reacted glass-ionomer (S-PRG) filler, produced by PRG technology for use with various dental materials, is bioactive and known to release ions from a glass-ionomer phase. We previously reported that coxsackievirus and adenovirus receptor (CXADR), a tight junction associated protein, was...
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| Veröffentlicht in: | PloS one 2022-07, Vol.17 (7), p.e0271192-e0271192 |
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| creator | Takeuchi, Hiroki Kato, Yuta Sasaki, Naoko Tanigaki, Keita Yamaga, Shunsuke Mita, Ena Kuboniwa, Masae Matsusaki, Michiya Amano, Atsuo |
| description | Surface pre-reacted glass-ionomer (S-PRG) filler, produced by PRG technology for use with various dental materials, is bioactive and known to release ions from a glass-ionomer phase. We previously reported that coxsackievirus and adenovirus receptor (CXADR), a tight junction associated protein, was located in the epithelial barrier of gingival epithelium. In the present study, the tissue protective effects of an S-PRG eluate prepared with S-PRG filler were investigated using a three-dimensional human gingival epithelial tissue model. The results showed that the S-PRG eluate specifically induced CXADR expression at the transcriptional level of messenger RNA as well as the protein level, and also nuclear translocation of transcription factor EB (TFEB) in gingival epithelial cells. Furthermore, shigyakusan, a TFEB inhibitor, canceled induction of the CXADR protein by the S-PRG eluate. Additionally, gingival epithelial permeation by 40-kDa dextran, lipopolysaccharide, and peptidoglycan in the 3D-tissue models was prevented by the eluate, with those effects abrogated by knockdown of CXADR. These findings suggest that S-PRG eluate increases CXADR expression via the TFEB pathway, thus inhibiting penetration of bacterial virulence factors into subepithelial tissues. |
| doi_str_mv | 10.1371/journal.pone.0271192 |
| format | Article |
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We previously reported that coxsackievirus and adenovirus receptor (CXADR), a tight junction associated protein, was located in the epithelial barrier of gingival epithelium. In the present study, the tissue protective effects of an S-PRG eluate prepared with S-PRG filler were investigated using a three-dimensional human gingival epithelial tissue model. The results showed that the S-PRG eluate specifically induced CXADR expression at the transcriptional level of messenger RNA as well as the protein level, and also nuclear translocation of transcription factor EB (TFEB) in gingival epithelial cells. Furthermore, shigyakusan, a TFEB inhibitor, canceled induction of the CXADR protein by the S-PRG eluate. Additionally, gingival epithelial permeation by 40-kDa dextran, lipopolysaccharide, and peptidoglycan in the 3D-tissue models was prevented by the eluate, with those effects abrogated by knockdown of CXADR. These findings suggest that S-PRG eluate increases CXADR expression via the TFEB pathway, thus inhibiting penetration of bacterial virulence factors into subepithelial tissues.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0271192</identifier><identifier>PMID: 35895663</identifier><language>eng</language><publisher>San Francisco: Public Library of Science</publisher><subject>Antimicrobial agents ; Autophagy ; Bacteria ; Biology and Life Sciences ; Complications and side effects ; Composite materials ; Coxsackievirus infections ; Dental materials ; Dextran ; Dextrans ; Diagnosis ; Drug resistance ; Epithelial cells ; Epithelium ; Fillers ; Fluorides ; Genetic aspects ; Gingiva ; Health aspects ; Ionomers ; Lipopolysaccharides ; Medicine and Health Sciences ; Microscopy ; mRNA ; Nuclear transport ; Pathogens ; Penetration ; Peptidoglycans ; Periodontal disease ; Proteins ; Research and Analysis Methods ; Solute movement ; Three dimensional models ; Tissues ; Transcription factors ; Translocation ; Virulence ; Virulence factors</subject><ispartof>PloS one, 2022-07, Vol.17 (7), p.e0271192-e0271192</ispartof><rights>COPYRIGHT 2022 Public Library of Science</rights><rights>2022 Takeuchi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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We previously reported that coxsackievirus and adenovirus receptor (CXADR), a tight junction associated protein, was located in the epithelial barrier of gingival epithelium. In the present study, the tissue protective effects of an S-PRG eluate prepared with S-PRG filler were investigated using a three-dimensional human gingival epithelial tissue model. The results showed that the S-PRG eluate specifically induced CXADR expression at the transcriptional level of messenger RNA as well as the protein level, and also nuclear translocation of transcription factor EB (TFEB) in gingival epithelial cells. Furthermore, shigyakusan, a TFEB inhibitor, canceled induction of the CXADR protein by the S-PRG eluate. Additionally, gingival epithelial permeation by 40-kDa dextran, lipopolysaccharide, and peptidoglycan in the 3D-tissue models was prevented by the eluate, with those effects abrogated by knockdown of CXADR. These findings suggest that S-PRG eluate increases CXADR expression via the TFEB pathway, thus inhibiting penetration of bacterial virulence factors into subepithelial tissues.</description><subject>Antimicrobial agents</subject><subject>Autophagy</subject><subject>Bacteria</subject><subject>Biology and Life Sciences</subject><subject>Complications and side effects</subject><subject>Composite materials</subject><subject>Coxsackievirus infections</subject><subject>Dental materials</subject><subject>Dextran</subject><subject>Dextrans</subject><subject>Diagnosis</subject><subject>Drug resistance</subject><subject>Epithelial cells</subject><subject>Epithelium</subject><subject>Fillers</subject><subject>Fluorides</subject><subject>Genetic aspects</subject><subject>Gingiva</subject><subject>Health aspects</subject><subject>Ionomers</subject><subject>Lipopolysaccharides</subject><subject>Medicine and Health Sciences</subject><subject>Microscopy</subject><subject>mRNA</subject><subject>Nuclear 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V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Surface pre-reacted glass-ionomer eluate protects gingival epithelium from penetration by lipopolysaccharides and peptidoglycans via transcription factor EB pathway</atitle><jtitle>PloS one</jtitle><date>2022-07-27</date><risdate>2022</risdate><volume>17</volume><issue>7</issue><spage>e0271192</spage><epage>e0271192</epage><pages>e0271192-e0271192</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Surface pre-reacted glass-ionomer (S-PRG) filler, produced by PRG technology for use with various dental materials, is bioactive and known to release ions from a glass-ionomer phase. We previously reported that coxsackievirus and adenovirus receptor (CXADR), a tight junction associated protein, was located in the epithelial barrier of gingival epithelium. In the present study, the tissue protective effects of an S-PRG eluate prepared with S-PRG filler were investigated using a three-dimensional human gingival epithelial tissue model. The results showed that the S-PRG eluate specifically induced CXADR expression at the transcriptional level of messenger RNA as well as the protein level, and also nuclear translocation of transcription factor EB (TFEB) in gingival epithelial cells. Furthermore, shigyakusan, a TFEB inhibitor, canceled induction of the CXADR protein by the S-PRG eluate. Additionally, gingival epithelial permeation by 40-kDa dextran, lipopolysaccharide, and peptidoglycan in the 3D-tissue models was prevented by the eluate, with those effects abrogated by knockdown of CXADR. These findings suggest that S-PRG eluate increases CXADR expression via the TFEB pathway, thus inhibiting penetration of bacterial virulence factors into subepithelial tissues.</abstract><cop>San Francisco</cop><pub>Public Library of Science</pub><pmid>35895663</pmid><doi>10.1371/journal.pone.0271192</doi><tpages>e0271192</tpages><orcidid>https://orcid.org/0000-0001-5938-1897</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Antimicrobial agents Autophagy Bacteria Biology and Life Sciences Complications and side effects Composite materials Coxsackievirus infections Dental materials Dextran Dextrans Diagnosis Drug resistance Epithelial cells Epithelium Fillers Fluorides Genetic aspects Gingiva Health aspects Ionomers Lipopolysaccharides Medicine and Health Sciences Microscopy mRNA Nuclear transport Pathogens Penetration Peptidoglycans Periodontal disease Proteins Research and Analysis Methods Solute movement Three dimensional models Tissues Transcription factors Translocation Virulence Virulence factors |
| title | Surface pre-reacted glass-ionomer eluate protects gingival epithelium from penetration by lipopolysaccharides and peptidoglycans via transcription factor EB pathway |
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