Determination of spider mite abundance in soil of field-grown cucumbers and in plants under predatory mite pressure in invasive infestations using HRM real-time PCR assay
The two spotted spider mite, Tetranychus urticae Koch L. (Acari: Tetranychidae), is a plant pest that can lead to severe economic losses in open field cucumber cultivation. Between 2017 and 2019 we studied the abundance of spider mites in the soil to estimate the potential infestation pressure of so...
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| Veröffentlicht in: | PloS one 2022-07, Vol.17 (7), p.e0270068-e0270068 |
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| creator | Kersten, Anne-Katrin Büttner, Carmen Lentzsch, Peter |
| description | The two spotted spider mite, Tetranychus urticae Koch L. (Acari: Tetranychidae), is a plant pest that can lead to severe economic losses in open field cucumber cultivation. Between 2017 and 2019 we studied the abundance of spider mites in the soil to estimate the potential infestation pressure of soil colonizing spider mites. The spider mites were heterogeneously distributed in small concentrations in the soil. Soil colonizing spider mites did not affect spider mite abundance on plants and reversed. We observed that spider mite migration occurred primarily from the edge of the field adjacent to the weed strip. In 2020 and 2021, we investigated the efficacy of the predatory mite Neoseiulus californicus (McGregor) for suppressing spider mite hotspots in the cropland. We compared untreated spider mite hotspots with N. californicus treated hotspots and showed that a single release of predatory mites could result in a high level of control when spider mite infestation density was initially high. With this study, soil can be ruled out as a habitat for spider mites, and attention to spider mite pest control can be directed to plant infestations. The highly sensitive HRM real-time PCR assay was used for the quantification of the spider mites. |
| doi_str_mv | 10.1371/journal.pone.0270068 |
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(Acari: Tetranychidae), is a plant pest that can lead to severe economic losses in open field cucumber cultivation. Between 2017 and 2019 we studied the abundance of spider mites in the soil to estimate the potential infestation pressure of soil colonizing spider mites. The spider mites were heterogeneously distributed in small concentrations in the soil. Soil colonizing spider mites did not affect spider mite abundance on plants and reversed. We observed that spider mite migration occurred primarily from the edge of the field adjacent to the weed strip. In 2020 and 2021, we investigated the efficacy of the predatory mite Neoseiulus californicus (McGregor) for suppressing spider mite hotspots in the cropland. We compared untreated spider mite hotspots with N. californicus treated hotspots and showed that a single release of predatory mites could result in a high level of control when spider mite infestation density was initially high. With this study, soil can be ruled out as a habitat for spider mites, and attention to spider mite pest control can be directed to plant infestations. The highly sensitive HRM real-time PCR assay was used for the quantification of the spider mites.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0270068</identifier><identifier>PMID: 35834527</identifier><language>eng</language><publisher>San Francisco: Public Library of Science</publisher><subject>Abundance ; Agricultural land ; Analysis ; Biological control ; Biology and Life Sciences ; Crop yields ; Cucumbers ; Earth Sciences ; Economic impact ; Growth ; Hot spots ; Infestation ; Leaves ; Management ; Mites ; Pest control ; Pests ; Physical Sciences ; Real time ; Research and Analysis Methods ; Soils ; Spiders ; Tetranychidae ; Vegetable cultivation</subject><ispartof>PloS one, 2022-07, Vol.17 (7), p.e0270068-e0270068</ispartof><rights>COPYRIGHT 2022 Public Library of Science</rights><rights>2022 Kersten et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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(Acari: Tetranychidae), is a plant pest that can lead to severe economic losses in open field cucumber cultivation. Between 2017 and 2019 we studied the abundance of spider mites in the soil to estimate the potential infestation pressure of soil colonizing spider mites. The spider mites were heterogeneously distributed in small concentrations in the soil. Soil colonizing spider mites did not affect spider mite abundance on plants and reversed. We observed that spider mite migration occurred primarily from the edge of the field adjacent to the weed strip. In 2020 and 2021, we investigated the efficacy of the predatory mite Neoseiulus californicus (McGregor) for suppressing spider mite hotspots in the cropland. We compared untreated spider mite hotspots with N. californicus treated hotspots and showed that a single release of predatory mites could result in a high level of control when spider mite infestation density was initially high. 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Carmen</au><au>Lentzsch, Peter</au><au>Ataide, Livia Maria Silva</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of spider mite abundance in soil of field-grown cucumbers and in plants under predatory mite pressure in invasive infestations using HRM real-time PCR assay</atitle><jtitle>PloS one</jtitle><date>2022-07-14</date><risdate>2022</risdate><volume>17</volume><issue>7</issue><spage>e0270068</spage><epage>e0270068</epage><pages>e0270068-e0270068</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The two spotted spider mite, Tetranychus urticae Koch L. (Acari: Tetranychidae), is a plant pest that can lead to severe economic losses in open field cucumber cultivation. Between 2017 and 2019 we studied the abundance of spider mites in the soil to estimate the potential infestation pressure of soil colonizing spider mites. The spider mites were heterogeneously distributed in small concentrations in the soil. Soil colonizing spider mites did not affect spider mite abundance on plants and reversed. We observed that spider mite migration occurred primarily from the edge of the field adjacent to the weed strip. In 2020 and 2021, we investigated the efficacy of the predatory mite Neoseiulus californicus (McGregor) for suppressing spider mite hotspots in the cropland. We compared untreated spider mite hotspots with N. californicus treated hotspots and showed that a single release of predatory mites could result in a high level of control when spider mite infestation density was initially high. 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| subjects | Abundance Agricultural land Analysis Biological control Biology and Life Sciences Crop yields Cucumbers Earth Sciences Economic impact Growth Hot spots Infestation Leaves Management Mites Pest control Pests Physical Sciences Real time Research and Analysis Methods Soils Spiders Tetranychidae Vegetable cultivation |
| title | Determination of spider mite abundance in soil of field-grown cucumbers and in plants under predatory mite pressure in invasive infestations using HRM real-time PCR assay |
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