In-vivo egfp expression in the honeybee Apis mellifera induced by electroporation and viral expression vector
In this study we describe egfp expression induced by two techniques: in vivo electroporation and viral transduction in several cell types of the adult honeybee brain. Non-neuronal and neuronal cell types were identified and the expression persisted at least during three days. Kenyon cells, optic lob...
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| Veröffentlicht in: | PloS one 2022-06, Vol.17 (6), p.e0263908-e0263908 |
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| creator | Leboulle, Gérard Gehne, Nora Froese, Anja Menzel, Randolf |
| description | In this study we describe egfp expression induced by two techniques: in vivo electroporation and viral transduction in several cell types of the adult honeybee brain. Non-neuronal and neuronal cell types were identified and the expression persisted at least during three days. Kenyon cells, optic lobe neurons and protocerebral lobe neurons were electroporated. Astrocyte-like glia cells, fibrous lamellar glia cells and cortex glia cells were identified. Viral transduction targeted one specific type of glia cells that could not be identified. EGFP positive cells types were rather variable after electroporation, and viral transduction resulted in more homogenous groups of positive cells. We propose that these techniques remain a good alternative to transgenic animals because they potentially target only somatic cells. |
| doi_str_mv | 10.1371/journal.pone.0263908 |
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Non-neuronal and neuronal cell types were identified and the expression persisted at least during three days. Kenyon cells, optic lobe neurons and protocerebral lobe neurons were electroporated. Astrocyte-like glia cells, fibrous lamellar glia cells and cortex glia cells were identified. Viral transduction targeted one specific type of glia cells that could not be identified. EGFP positive cells types were rather variable after electroporation, and viral transduction resulted in more homogenous groups of positive cells. We propose that these techniques remain a good alternative to transgenic animals because they potentially target only somatic cells.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0263908</identifier><identifier>PMID: 35653376</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Antibodies ; Bees ; Biology and Life Sciences ; Brain ; Electrodes ; Electroporation ; Enhanced green fluorescent protein ; Gene expression ; Genomes ; Glycerol ; Honeybee ; In vivo methods and tests ; Membranes ; Methods ; Neurological research ; Neuronal-glial interactions ; Neurons ; Neurosciences ; Optic lobe ; Physical Sciences ; Physiological aspects ; Physiology ; Research and Analysis Methods ; Somatic cells ; Testing ; Transduction ; Transgenic animals ; Vectors (Biology) ; Wire</subject><ispartof>PloS one, 2022-06, Vol.17 (6), p.e0263908-e0263908</ispartof><rights>COPYRIGHT 2022 Public Library of Science</rights><rights>2022 Leboulle et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 Leboulle et al 2022 Leboulle et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c641t-a756e548194eebb6efc672f66d719956f302cee93184e70be9445d390bca299e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162312/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162312/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53770,53772,79347,79348</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35653376$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Roman, Gregg</contributor><creatorcontrib>Leboulle, Gérard</creatorcontrib><creatorcontrib>Gehne, Nora</creatorcontrib><creatorcontrib>Froese, Anja</creatorcontrib><creatorcontrib>Menzel, Randolf</creatorcontrib><title>In-vivo egfp expression in the honeybee Apis mellifera induced by electroporation and viral expression vector</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>In this study we describe egfp expression induced by two techniques: in vivo electroporation and viral transduction in several cell types of the adult honeybee brain. Non-neuronal and neuronal cell types were identified and the expression persisted at least during three days. Kenyon cells, optic lobe neurons and protocerebral lobe neurons were electroporated. Astrocyte-like glia cells, fibrous lamellar glia cells and cortex glia cells were identified. Viral transduction targeted one specific type of glia cells that could not be identified. EGFP positive cells types were rather variable after electroporation, and viral transduction resulted in more homogenous groups of positive cells. We propose that these techniques remain a good alternative to transgenic animals because they potentially target only somatic cells.</description><subject>Antibodies</subject><subject>Bees</subject><subject>Biology and Life Sciences</subject><subject>Brain</subject><subject>Electrodes</subject><subject>Electroporation</subject><subject>Enhanced green fluorescent protein</subject><subject>Gene expression</subject><subject>Genomes</subject><subject>Glycerol</subject><subject>Honeybee</subject><subject>In vivo methods and tests</subject><subject>Membranes</subject><subject>Methods</subject><subject>Neurological research</subject><subject>Neuronal-glial interactions</subject><subject>Neurons</subject><subject>Neurosciences</subject><subject>Optic lobe</subject><subject>Physical Sciences</subject><subject>Physiological aspects</subject><subject>Physiology</subject><subject>Research and Analysis Methods</subject><subject>Somatic 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one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Leboulle, Gérard</au><au>Gehne, Nora</au><au>Froese, Anja</au><au>Menzel, Randolf</au><au>Roman, Gregg</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In-vivo egfp expression in the honeybee Apis mellifera induced by electroporation and viral expression vector</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2022-06-02</date><risdate>2022</risdate><volume>17</volume><issue>6</issue><spage>e0263908</spage><epage>e0263908</epage><pages>e0263908-e0263908</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>In this study we describe egfp expression induced by two techniques: in vivo electroporation and viral transduction in several cell types of the adult honeybee brain. Non-neuronal and neuronal cell types were identified and the expression persisted at least during three days. Kenyon cells, optic lobe neurons and protocerebral lobe neurons were electroporated. Astrocyte-like glia cells, fibrous lamellar glia cells and cortex glia cells were identified. Viral transduction targeted one specific type of glia cells that could not be identified. EGFP positive cells types were rather variable after electroporation, and viral transduction resulted in more homogenous groups of positive cells. We propose that these techniques remain a good alternative to transgenic animals because they potentially target only somatic cells.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>35653376</pmid><doi>10.1371/journal.pone.0263908</doi><tpages>e0263908</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Antibodies Bees Biology and Life Sciences Brain Electrodes Electroporation Enhanced green fluorescent protein Gene expression Genomes Glycerol Honeybee In vivo methods and tests Membranes Methods Neurological research Neuronal-glial interactions Neurons Neurosciences Optic lobe Physical Sciences Physiological aspects Physiology Research and Analysis Methods Somatic cells Testing Transduction Transgenic animals Vectors (Biology) Wire |
| title | In-vivo egfp expression in the honeybee Apis mellifera induced by electroporation and viral expression vector |
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