Novel reusable animal model for comparative evaluation of in vivo growth and protein-expression of Escherichia coli O157 strains in the bovine rumen
Previously, we had demonstrated that Escherichia coli O157:H7 (O157) strain 86-24 expresses proteins involved in survival rather than virulence in vitro in rumen fluid from dairy cattle limit fed a maintenance diet. Here, we verified if this observation would be true for different O157 strains grown...
Gespeichert in:
| Veröffentlicht in: | PloS one 2022-05, Vol.17 (5), p.e0268645-e0268645 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0268645 |
|---|---|
| container_issue | 5 |
| container_start_page | e0268645 |
| container_title | PloS one |
| container_volume | 17 |
| creator | Kudva, Indira T Trachsel, Julian Biernbaum, Erika N Casey, Thomas |
| description | Previously, we had demonstrated that Escherichia coli O157:H7 (O157) strain 86-24 expresses proteins involved in survival rather than virulence in vitro in rumen fluid from dairy cattle limit fed a maintenance diet. Here, we verified if this observation would be true for different O157 strains grown in vitro in rumen fluid from, and in vivo in the rumen of, animals on contrasting maintenance (high fiber) and lactation (high energy-protein) diets usually limit fed to dairy cattle. For the in vivo studies, an economical, novel, reusable and non-terminal rumen-fistulated animal model permitting simultaneous evaluation of multiple bacterial strains in the bovine rumen was developed. All experiments were conducted in duplicate using different animals to account for host-related variations. The O157 strains included, 86-24, EDL933 and the super shed SS-17. E. coli NalR (#5735), derived from a bovine intestinal commensal E. coli, was included as a control. As expected, diet influenced ruminal pH and volatile fatty acid (VFA) composition. The pH ranged from 6.2-7.0 and total VFA concentrations from 109-141 μM/ml, in animals fed the maintenance diet. In comparison, animals fed the lactation diet had a ruminal pH ranging between 5.18-6.0, and total VFA of 125-219 μM/ml. Strain dependent differences in O157 recovery from the rumen fluid of cattle fed either diet was observed, both in vitro and in vivo, with O157 strains 86-24 and EDL933 demonstrating similar survival patterns. Analysis of the O157 proteomes expressed in the rumen fluid/rumen verified previous observations of adaptive responses. Any difference in the adaptive response was mainly influenced by the animal's diet and growth conditions (in vitro and in vivo) and not the O157 strain. These new insights into the O157 responses could help formulate modalities to control O157 across strains in cattle at all stages of husbandry. |
| doi_str_mv | 10.1371/journal.pone.0268645 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2686266820</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A705025438</galeid><doaj_id>oai_doaj_org_article_789ebd78ecc9428bb38977d4489353a5</doaj_id><sourcerecordid>A705025438</sourcerecordid><originalsourceid>FETCH-LOGICAL-c719t-67f1bcc6b804aaf90b406ad6dc30db249b0d5f0a2efe93c37f077ea7ce84ed7f3</originalsourceid><addsrcrecordid>eNqNk9-K1DAUxoso7jr6BqJBQfRixrRpk_ZGWJZVBxYH_Hcb0vR0miVNxiSt63v4wKZOd5mRvZBcNJz-zpdzvuQkydMUr1LC0rdXdnBG6NXOGljhjJY0L-4lp2lFsiXNMLl_sD9JHnl_hXFBSkofJiekoCkjlJ4mvz_ZETRyMHhRa0DCqF5o1NsmRlvrkLT9TjgR1AgIRqGHuLUG2RYpg0Y1WrR19mfoYmaDds4GUGYJ1zsH3s_ghZcdOCU7JaKcVmiTFgz54IQyfpIJHaDajsoAckMP5nHyoBXaw5P5u0i-vb_4ev5xebn5sD4_u1xKllZhSVmb1lLSusS5EG2F6xxT0dBGEtzUWV7VuClaLDJooSKSsBYzBoJJKHNoWEsWyfO97k5bz2dDPZ-8zCgto3GLZL0nGiuu-M5Fc9wvboXifwPWbblwQUkNnJUV1A0rQcoqz8q6JmXFWJPnZUUKIoqo9W4-bah7aCSY6IA-Ej3-Y1THt3bkVUqKLCujwIu9gPVBcS9VANlJawzIwNMK5xWlEXo9n-LsjwF84L3yErQWBuwwNcfS2CDOp4Je_oPebcFMbUXsUpnWxuLkJMrPGC5wVuRkqm11BxVXA72KNUKrYvwo4c1RQmQCXIetGLzn6y-f_5_dfD9mXx2wHQgdOm_1ML1afwzme1A6672D9vYmUsynCbtxg08TxucJi2nPDm_xNulmpMgfhfYi3Q</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2686266820</pqid></control><display><type>article</type><title>Novel reusable animal model for comparative evaluation of in vivo growth and protein-expression of Escherichia coli O157 strains in the bovine rumen</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Kudva, Indira T ; Trachsel, Julian ; Biernbaum, Erika N ; Casey, Thomas</creator><contributor>Germon, Pierre</contributor><creatorcontrib>Kudva, Indira T ; Trachsel, Julian ; Biernbaum, Erika N ; Casey, Thomas ; Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States) ; Germon, Pierre</creatorcontrib><description>Previously, we had demonstrated that Escherichia coli O157:H7 (O157) strain 86-24 expresses proteins involved in survival rather than virulence in vitro in rumen fluid from dairy cattle limit fed a maintenance diet. Here, we verified if this observation would be true for different O157 strains grown in vitro in rumen fluid from, and in vivo in the rumen of, animals on contrasting maintenance (high fiber) and lactation (high energy-protein) diets usually limit fed to dairy cattle. For the in vivo studies, an economical, novel, reusable and non-terminal rumen-fistulated animal model permitting simultaneous evaluation of multiple bacterial strains in the bovine rumen was developed. All experiments were conducted in duplicate using different animals to account for host-related variations. The O157 strains included, 86-24, EDL933 and the super shed SS-17. E. coli NalR (#5735), derived from a bovine intestinal commensal E. coli, was included as a control. As expected, diet influenced ruminal pH and volatile fatty acid (VFA) composition. The pH ranged from 6.2-7.0 and total VFA concentrations from 109-141 μM/ml, in animals fed the maintenance diet. In comparison, animals fed the lactation diet had a ruminal pH ranging between 5.18-6.0, and total VFA of 125-219 μM/ml. Strain dependent differences in O157 recovery from the rumen fluid of cattle fed either diet was observed, both in vitro and in vivo, with O157 strains 86-24 and EDL933 demonstrating similar survival patterns. Analysis of the O157 proteomes expressed in the rumen fluid/rumen verified previous observations of adaptive responses. Any difference in the adaptive response was mainly influenced by the animal's diet and growth conditions (in vitro and in vivo) and not the O157 strain. These new insights into the O157 responses could help formulate modalities to control O157 across strains in cattle at all stages of husbandry.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0268645</identifier><identifier>PMID: 35617366</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animal experimentation ; Animal husbandry ; Animal lactation ; Animal models ; Animals ; artificial membranes ; Bacteria ; BASIC BIOLOGICAL SCIENCES ; Biology and Life Sciences ; Cattle ; cell membranes ; Chemical composition ; cytoplasm ; Dairy cattle ; Diet ; Diet - veterinary ; E coli ; Economic models ; Epidemics ; Escherichia coli ; Escherichia coli O157 - physiology ; Evaluation ; Experiments ; Fatty acids ; Fatty Acids, Volatile - analysis ; Feces ; Feces - microbiology ; Feeds ; Female ; Food contamination & poisoning ; Genetic aspects ; Growth conditions ; Hemodialysis ; High protein diet ; Identification and classification ; In vivo methods and tests ; Lactation ; Maintenance ; Medicine and Health Sciences ; membrane metabolism ; Models, Animal ; nuclear membrane ; outer membrane proteins ; pH effects ; Physical Sciences ; Probiotics ; Properties ; protein expression ; Proteins ; Proteomes ; Research and Analysis Methods ; Rumen ; Rumen - microbiology ; Strains (organisms) ; Survival ; Virulence</subject><ispartof>PloS one, 2022-05, Vol.17 (5), p.e0268645-e0268645</ispartof><rights>COPYRIGHT 2022 Public Library of Science</rights><rights>This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication: https://creativecommons.org/publicdomain/zero/1.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c719t-67f1bcc6b804aaf90b406ad6dc30db249b0d5f0a2efe93c37f077ea7ce84ed7f3</citedby><cites>FETCH-LOGICAL-c719t-67f1bcc6b804aaf90b406ad6dc30db249b0d5f0a2efe93c37f077ea7ce84ed7f3</cites><orcidid>0000-0001-6750-7867 ; 0000-0003-2357-7737 ; 0000000323577737 ; 0000000167507867</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9135228/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9135228/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35617366$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/servlets/purl/1904966$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><contributor>Germon, Pierre</contributor><creatorcontrib>Kudva, Indira T</creatorcontrib><creatorcontrib>Trachsel, Julian</creatorcontrib><creatorcontrib>Biernbaum, Erika N</creatorcontrib><creatorcontrib>Casey, Thomas</creatorcontrib><creatorcontrib>Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)</creatorcontrib><title>Novel reusable animal model for comparative evaluation of in vivo growth and protein-expression of Escherichia coli O157 strains in the bovine rumen</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Previously, we had demonstrated that Escherichia coli O157:H7 (O157) strain 86-24 expresses proteins involved in survival rather than virulence in vitro in rumen fluid from dairy cattle limit fed a maintenance diet. Here, we verified if this observation would be true for different O157 strains grown in vitro in rumen fluid from, and in vivo in the rumen of, animals on contrasting maintenance (high fiber) and lactation (high energy-protein) diets usually limit fed to dairy cattle. For the in vivo studies, an economical, novel, reusable and non-terminal rumen-fistulated animal model permitting simultaneous evaluation of multiple bacterial strains in the bovine rumen was developed. All experiments were conducted in duplicate using different animals to account for host-related variations. The O157 strains included, 86-24, EDL933 and the super shed SS-17. E. coli NalR (#5735), derived from a bovine intestinal commensal E. coli, was included as a control. As expected, diet influenced ruminal pH and volatile fatty acid (VFA) composition. The pH ranged from 6.2-7.0 and total VFA concentrations from 109-141 μM/ml, in animals fed the maintenance diet. In comparison, animals fed the lactation diet had a ruminal pH ranging between 5.18-6.0, and total VFA of 125-219 μM/ml. Strain dependent differences in O157 recovery from the rumen fluid of cattle fed either diet was observed, both in vitro and in vivo, with O157 strains 86-24 and EDL933 demonstrating similar survival patterns. Analysis of the O157 proteomes expressed in the rumen fluid/rumen verified previous observations of adaptive responses. Any difference in the adaptive response was mainly influenced by the animal's diet and growth conditions (in vitro and in vivo) and not the O157 strain. These new insights into the O157 responses could help formulate modalities to control O157 across strains in cattle at all stages of husbandry.</description><subject>Analysis</subject><subject>Animal experimentation</subject><subject>Animal husbandry</subject><subject>Animal lactation</subject><subject>Animal models</subject><subject>Animals</subject><subject>artificial membranes</subject><subject>Bacteria</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>Biology and Life Sciences</subject><subject>Cattle</subject><subject>cell membranes</subject><subject>Chemical composition</subject><subject>cytoplasm</subject><subject>Dairy cattle</subject><subject>Diet</subject><subject>Diet - veterinary</subject><subject>E coli</subject><subject>Economic models</subject><subject>Epidemics</subject><subject>Escherichia coli</subject><subject>Escherichia coli O157 - physiology</subject><subject>Evaluation</subject><subject>Experiments</subject><subject>Fatty acids</subject><subject>Fatty Acids, Volatile - analysis</subject><subject>Feces</subject><subject>Feces - microbiology</subject><subject>Feeds</subject><subject>Female</subject><subject>Food contamination & poisoning</subject><subject>Genetic aspects</subject><subject>Growth conditions</subject><subject>Hemodialysis</subject><subject>High protein diet</subject><subject>Identification and classification</subject><subject>In vivo methods and tests</subject><subject>Lactation</subject><subject>Maintenance</subject><subject>Medicine and Health Sciences</subject><subject>membrane metabolism</subject><subject>Models, Animal</subject><subject>nuclear membrane</subject><subject>outer membrane proteins</subject><subject>pH effects</subject><subject>Physical Sciences</subject><subject>Probiotics</subject><subject>Properties</subject><subject>protein expression</subject><subject>Proteins</subject><subject>Proteomes</subject><subject>Research and Analysis Methods</subject><subject>Rumen</subject><subject>Rumen - microbiology</subject><subject>Strains (organisms)</subject><subject>Survival</subject><subject>Virulence</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9-K1DAUxoso7jr6BqJBQfRixrRpk_ZGWJZVBxYH_Hcb0vR0miVNxiSt63v4wKZOd5mRvZBcNJz-zpdzvuQkydMUr1LC0rdXdnBG6NXOGljhjJY0L-4lp2lFsiXNMLl_sD9JHnl_hXFBSkofJiekoCkjlJ4mvz_ZETRyMHhRa0DCqF5o1NsmRlvrkLT9TjgR1AgIRqGHuLUG2RYpg0Y1WrR19mfoYmaDds4GUGYJ1zsH3s_ghZcdOCU7JaKcVmiTFgz54IQyfpIJHaDajsoAckMP5nHyoBXaw5P5u0i-vb_4ev5xebn5sD4_u1xKllZhSVmb1lLSusS5EG2F6xxT0dBGEtzUWV7VuClaLDJooSKSsBYzBoJJKHNoWEsWyfO97k5bz2dDPZ-8zCgto3GLZL0nGiuu-M5Fc9wvboXifwPWbblwQUkNnJUV1A0rQcoqz8q6JmXFWJPnZUUKIoqo9W4-bah7aCSY6IA-Ej3-Y1THt3bkVUqKLCujwIu9gPVBcS9VANlJawzIwNMK5xWlEXo9n-LsjwF84L3yErQWBuwwNcfS2CDOp4Je_oPebcFMbUXsUpnWxuLkJMrPGC5wVuRkqm11BxVXA72KNUKrYvwo4c1RQmQCXIetGLzn6y-f_5_dfD9mXx2wHQgdOm_1ML1afwzme1A6672D9vYmUsynCbtxg08TxucJi2nPDm_xNulmpMgfhfYi3Q</recordid><startdate>20220526</startdate><enddate>20220526</enddate><creator>Kudva, Indira T</creator><creator>Trachsel, Julian</creator><creator>Biernbaum, Erika N</creator><creator>Casey, Thomas</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>OIOZB</scope><scope>OTOTI</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-6750-7867</orcidid><orcidid>https://orcid.org/0000-0003-2357-7737</orcidid><orcidid>https://orcid.org/0000000323577737</orcidid><orcidid>https://orcid.org/0000000167507867</orcidid></search><sort><creationdate>20220526</creationdate><title>Novel reusable animal model for comparative evaluation of in vivo growth and protein-expression of Escherichia coli O157 strains in the bovine rumen</title><author>Kudva, Indira T ; Trachsel, Julian ; Biernbaum, Erika N ; Casey, Thomas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c719t-67f1bcc6b804aaf90b406ad6dc30db249b0d5f0a2efe93c37f077ea7ce84ed7f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Analysis</topic><topic>Animal experimentation</topic><topic>Animal husbandry</topic><topic>Animal lactation</topic><topic>Animal models</topic><topic>Animals</topic><topic>artificial membranes</topic><topic>Bacteria</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>Biology and Life Sciences</topic><topic>Cattle</topic><topic>cell membranes</topic><topic>Chemical composition</topic><topic>cytoplasm</topic><topic>Dairy cattle</topic><topic>Diet</topic><topic>Diet - veterinary</topic><topic>E coli</topic><topic>Economic models</topic><topic>Epidemics</topic><topic>Escherichia coli</topic><topic>Escherichia coli O157 - physiology</topic><topic>Evaluation</topic><topic>Experiments</topic><topic>Fatty acids</topic><topic>Fatty Acids, Volatile - analysis</topic><topic>Feces</topic><topic>Feces - microbiology</topic><topic>Feeds</topic><topic>Female</topic><topic>Food contamination & poisoning</topic><topic>Genetic aspects</topic><topic>Growth conditions</topic><topic>Hemodialysis</topic><topic>High protein diet</topic><topic>Identification and classification</topic><topic>In vivo methods and tests</topic><topic>Lactation</topic><topic>Maintenance</topic><topic>Medicine and Health Sciences</topic><topic>membrane metabolism</topic><topic>Models, Animal</topic><topic>nuclear membrane</topic><topic>outer membrane proteins</topic><topic>pH effects</topic><topic>Physical Sciences</topic><topic>Probiotics</topic><topic>Properties</topic><topic>protein expression</topic><topic>Proteins</topic><topic>Proteomes</topic><topic>Research and Analysis Methods</topic><topic>Rumen</topic><topic>Rumen - microbiology</topic><topic>Strains (organisms)</topic><topic>Survival</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kudva, Indira T</creatorcontrib><creatorcontrib>Trachsel, Julian</creatorcontrib><creatorcontrib>Biernbaum, Erika N</creatorcontrib><creatorcontrib>Casey, Thomas</creatorcontrib><creatorcontrib>Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV - Hybrid</collection><collection>OSTI.GOV</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kudva, Indira T</au><au>Trachsel, Julian</au><au>Biernbaum, Erika N</au><au>Casey, Thomas</au><au>Germon, Pierre</au><aucorp>Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel reusable animal model for comparative evaluation of in vivo growth and protein-expression of Escherichia coli O157 strains in the bovine rumen</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2022-05-26</date><risdate>2022</risdate><volume>17</volume><issue>5</issue><spage>e0268645</spage><epage>e0268645</epage><pages>e0268645-e0268645</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Previously, we had demonstrated that Escherichia coli O157:H7 (O157) strain 86-24 expresses proteins involved in survival rather than virulence in vitro in rumen fluid from dairy cattle limit fed a maintenance diet. Here, we verified if this observation would be true for different O157 strains grown in vitro in rumen fluid from, and in vivo in the rumen of, animals on contrasting maintenance (high fiber) and lactation (high energy-protein) diets usually limit fed to dairy cattle. For the in vivo studies, an economical, novel, reusable and non-terminal rumen-fistulated animal model permitting simultaneous evaluation of multiple bacterial strains in the bovine rumen was developed. All experiments were conducted in duplicate using different animals to account for host-related variations. The O157 strains included, 86-24, EDL933 and the super shed SS-17. E. coli NalR (#5735), derived from a bovine intestinal commensal E. coli, was included as a control. As expected, diet influenced ruminal pH and volatile fatty acid (VFA) composition. The pH ranged from 6.2-7.0 and total VFA concentrations from 109-141 μM/ml, in animals fed the maintenance diet. In comparison, animals fed the lactation diet had a ruminal pH ranging between 5.18-6.0, and total VFA of 125-219 μM/ml. Strain dependent differences in O157 recovery from the rumen fluid of cattle fed either diet was observed, both in vitro and in vivo, with O157 strains 86-24 and EDL933 demonstrating similar survival patterns. Analysis of the O157 proteomes expressed in the rumen fluid/rumen verified previous observations of adaptive responses. Any difference in the adaptive response was mainly influenced by the animal's diet and growth conditions (in vitro and in vivo) and not the O157 strain. These new insights into the O157 responses could help formulate modalities to control O157 across strains in cattle at all stages of husbandry.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>35617366</pmid><doi>10.1371/journal.pone.0268645</doi><tpages>e0268645</tpages><orcidid>https://orcid.org/0000-0001-6750-7867</orcidid><orcidid>https://orcid.org/0000-0003-2357-7737</orcidid><orcidid>https://orcid.org/0000000323577737</orcidid><orcidid>https://orcid.org/0000000167507867</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2022-05, Vol.17 (5), p.e0268645-e0268645 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2686266820 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis Animal experimentation Animal husbandry Animal lactation Animal models Animals artificial membranes Bacteria BASIC BIOLOGICAL SCIENCES Biology and Life Sciences Cattle cell membranes Chemical composition cytoplasm Dairy cattle Diet Diet - veterinary E coli Economic models Epidemics Escherichia coli Escherichia coli O157 - physiology Evaluation Experiments Fatty acids Fatty Acids, Volatile - analysis Feces Feces - microbiology Feeds Female Food contamination & poisoning Genetic aspects Growth conditions Hemodialysis High protein diet Identification and classification In vivo methods and tests Lactation Maintenance Medicine and Health Sciences membrane metabolism Models, Animal nuclear membrane outer membrane proteins pH effects Physical Sciences Probiotics Properties protein expression Proteins Proteomes Research and Analysis Methods Rumen Rumen - microbiology Strains (organisms) Survival Virulence |
| title | Novel reusable animal model for comparative evaluation of in vivo growth and protein-expression of Escherichia coli O157 strains in the bovine rumen |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T05%3A00%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Novel%20reusable%20animal%20model%20for%20comparative%20evaluation%20of%20in%20vivo%20growth%20and%20protein-expression%20of%20Escherichia%20coli%20O157%20strains%20in%20the%20bovine%20rumen&rft.jtitle=PloS%20one&rft.au=Kudva,%20Indira%20T&rft.aucorp=Oak%20Ridge%20Institute%20for%20Science%20and%20Education%20(ORISE),%20Oak%20Ridge,%20TN%20(United%20States)&rft.date=2022-05-26&rft.volume=17&rft.issue=5&rft.spage=e0268645&rft.epage=e0268645&rft.pages=e0268645-e0268645&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0268645&rft_dat=%3Cgale_plos_%3EA705025438%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2686266820&rft_id=info:pmid/35617366&rft_galeid=A705025438&rft_doaj_id=oai_doaj_org_article_789ebd78ecc9428bb38977d4489353a5&rfr_iscdi=true |