Quantitative and qualitative evaluation of the hippocampal cytoarchitecture in adult cats with regard to the pathological diagnosis of hippocampal sclerosis
Cats are known to be affected by hippocampal sclerosis, potentially causing antiseizure drug(s) resistance. In order to lay the foundation for a standardized, systematic classification and diagnosis of this pathology in cats, this prospective study aimed at evaluating normal reference values of cell...
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| Veröffentlicht in: | PloS one 2022-05, Vol.17 (5), p.e0268010-e0268010 |
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| creator | Zilli, Jessica Schänzer, Anne Büttner, Kathrin Kressin, Monika Schmidt, Martin J |
| description | Cats are known to be affected by hippocampal sclerosis, potentially causing antiseizure drug(s) resistance. In order to lay the foundation for a standardized, systematic classification and diagnosis of this pathology in cats, this prospective study aimed at evaluating normal reference values of cellular densities and the cytoarchitecture of the feline hippocampus. Three transverse sections (head, body and tail) of each left hippocampus were obtained from 17 non-epileptic cats of different brachycephalic and mesocephalic breeds and age classes (range: 3-17 years). Histological (hematoxylin and eosin, Nissl) and immunohistochemical (NeuN, GFAP) staining was performed to investigate neuron and astroglial cell populations, as well as the layer thickness of the pyramidal cell layer and granule cell layer. Significant differences in neuronal density (in CA2-CA4 and the granule cell layer) and layer thickness (in CA1-CA3 and the granule cell layer) were evidenced throughout the longitudinal hippocampal axis (p |
| doi_str_mv | 10.1371/journal.pone.0268010 |
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In order to lay the foundation for a standardized, systematic classification and diagnosis of this pathology in cats, this prospective study aimed at evaluating normal reference values of cellular densities and the cytoarchitecture of the feline hippocampus. Three transverse sections (head, body and tail) of each left hippocampus were obtained from 17 non-epileptic cats of different brachycephalic and mesocephalic breeds and age classes (range: 3-17 years). Histological (hematoxylin and eosin, Nissl) and immunohistochemical (NeuN, GFAP) staining was performed to investigate neuron and astroglial cell populations, as well as the layer thickness of the pyramidal cell layer and granule cell layer. Significant differences in neuronal density (in CA2-CA4 and the granule cell layer) and layer thickness (in CA1-CA3 and the granule cell layer) were evidenced throughout the longitudinal hippocampal axis (p<0.05); on the other hand, the astrocyte density did not differ. Moreover, reference ranges were defined for these parameters in the pyramidal cell layer and in the granule cell layer. The findings did not differ according to breed or age. In veterinary medicine these parameters have not been evaluated in cats so far. As surgical treatment may become a therapeutic option for cats with temporal lobe epilepsy, estimating normal values of the hippocampal cytoarchitecture will help in the standardized histopathological examination of resected hippocampal specimens to reach a diagnosis of hippocampal sclerosis.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0268010</identifier><identifier>PMID: 35560321</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animal euthanasia ; Animals ; Biology and Life Sciences ; Brain ; Brain architecture ; Cadavers ; Cats ; Convulsions & seizures ; Density ; Diagnosis ; Diseases ; Epilepsy ; Epilepsy, Temporal Lobe - pathology ; Evaluation ; Females ; Glial fibrillary acidic protein ; Gliosis - pathology ; Granular materials ; Health aspects ; Hippocampus ; Hippocampus (Brain) ; Hippocampus - pathology ; Laboratories ; Magnetic resonance imaging ; Males ; Medical diagnosis ; Medicine and Health Sciences ; Neurodegenerative Diseases - pathology ; Neurons - pathology ; Parameters ; Pathology ; Physiological aspects ; Pituitary gland ; Prospective Studies ; Psychological aspects ; Research and Analysis Methods ; Sclerosis ; Sclerosis - pathology ; Temporal lobe ; Thickness ; Veterinary medicine</subject><ispartof>PloS one, 2022-05, Vol.17 (5), p.e0268010-e0268010</ispartof><rights>COPYRIGHT 2022 Public Library of Science</rights><rights>2022 Zilli et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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In order to lay the foundation for a standardized, systematic classification and diagnosis of this pathology in cats, this prospective study aimed at evaluating normal reference values of cellular densities and the cytoarchitecture of the feline hippocampus. Three transverse sections (head, body and tail) of each left hippocampus were obtained from 17 non-epileptic cats of different brachycephalic and mesocephalic breeds and age classes (range: 3-17 years). Histological (hematoxylin and eosin, Nissl) and immunohistochemical (NeuN, GFAP) staining was performed to investigate neuron and astroglial cell populations, as well as the layer thickness of the pyramidal cell layer and granule cell layer. Significant differences in neuronal density (in CA2-CA4 and the granule cell layer) and layer thickness (in CA1-CA3 and the granule cell layer) were evidenced throughout the longitudinal hippocampal axis (p<0.05); on the other hand, the astrocyte density did not differ. Moreover, reference ranges were defined for these parameters in the pyramidal cell layer and in the granule cell layer. The findings did not differ according to breed or age. In veterinary medicine these parameters have not been evaluated in cats so far. As surgical treatment may become a therapeutic option for cats with temporal lobe epilepsy, estimating normal values of the hippocampal cytoarchitecture will help in the standardized histopathological examination of resected hippocampal specimens to reach a diagnosis of hippocampal sclerosis.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>35560321</pmid><doi>10.1371/journal.pone.0268010</doi><tpages>e0268010</tpages><orcidid>https://orcid.org/0000-0003-4112-6518</orcidid><oa>free_for_read</oa></addata></record> |
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| source | PLoS; MEDLINE; Full-Text Journals in Chemistry (Open access); Directory of Open Access Journals - May need to register for free articles; PubMed Central; EZB Electronic Journals Library |
| subjects | Animal euthanasia Animals Biology and Life Sciences Brain Brain architecture Cadavers Cats Convulsions & seizures Density Diagnosis Diseases Epilepsy Epilepsy, Temporal Lobe - pathology Evaluation Females Glial fibrillary acidic protein Gliosis - pathology Granular materials Health aspects Hippocampus Hippocampus (Brain) Hippocampus - pathology Laboratories Magnetic resonance imaging Males Medical diagnosis Medicine and Health Sciences Neurodegenerative Diseases - pathology Neurons - pathology Parameters Pathology Physiological aspects Pituitary gland Prospective Studies Psychological aspects Research and Analysis Methods Sclerosis Sclerosis - pathology Temporal lobe Thickness Veterinary medicine |
| title | Quantitative and qualitative evaluation of the hippocampal cytoarchitecture in adult cats with regard to the pathological diagnosis of hippocampal sclerosis |
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