Demonstrating the utility of Escherichia coli asymptomatic bacteriuria isolates' virulence profile towards diagnosis and management-A preliminary analysis
Asymptomatic bacteriuria (ASB) caused by Escherichia coli (E. coli) is a significant condition associated with pregnancy and is considered as prognostic for the development of symptomatic urinary tract infection (UTI). However, treating all ASB increases the use of antibiotics and leads to the devel...
Gespeichert in:
| Veröffentlicht in: | PloS one 2022-05, Vol.17 (5), p.e0267296-e0267296 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0267296 |
|---|---|
| container_issue | 5 |
| container_start_page | e0267296 |
| container_title | PloS one |
| container_volume | 17 |
| creator | Maniam, Lalitha Vellasamy, Kumutha Malar Jindal, Hassan Mahmood Narayanan, Vallikannu Danaee, Mahmoud Vadivelu, Jamuna Pallath, Vinod |
| description | Asymptomatic bacteriuria (ASB) caused by Escherichia coli (E. coli) is a significant condition associated with pregnancy and is considered as prognostic for the development of symptomatic urinary tract infection (UTI). However, treating all ASB increases the use of antibiotics and leads to the development of multidrug resistance (MDR). Therefore, this study aimed to identify the distribution of UPEC associated virulence genes and antibiotic susceptibility among phylogroups of E. coli isolated from ASB in pregnancy. Moreover, the gene expression of selected virulence genes was also compared among two E. coli isolates (with different pathogenic potential) to determine its pathogenicity. One hundred and sixty E. coli isolates from midstream urine samples of pregnant women with ASB were subjected to PCR-based detection for its phylogroups and virulence genes. The antibiotic susceptibility of isolated strains was determined by the disc diffusion method. Expression of the virulence genes were determined through microarray analysis and quantitative Real-Time PCR. The prevalence of ASB in this study was 16.1%. Within ASB isolates, the occurrence of phylogroup B2 was the highest, and isolates from this group harboured most of the virulence genes studied. Overall, the most identified virulence genes among all phylogroups in descending order were fimH, chuA, kpsMTII, usp, fyuA, hlyA, iroN, cnf, papC, sfa, ompT, and sat. In this study, higher resistance to antibiotics was observed for ampicillin (77.5%), amoxicillin-clavulanate (54.4%), trimethoprim-sulfamethoxazole (46.9%) and amikacin (43.8%) compared to the other tested antibiotics and 51.9% of the tested isolates were MDR. Furthermore, hierarchical clustering and gene expression analysis demonstrated extreme polarization of pathogenic potential of E. coli causing ASB in pregnancy necessitating the need for bacterial isolate focused approach towards treatment of ASB. |
| doi_str_mv | 10.1371/journal.pone.0267296 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2686209033</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A702727020</galeid><doaj_id>oai_doaj_org_article_8568e809e2f746c9abd38d8651d2145c</doaj_id><sourcerecordid>A702727020</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-ff02dd3ba68bd023d3829089fdc46a76839d1795aa617b0de0709b29125e2c7d3</originalsourceid><addsrcrecordid>eNqNk9tu1DAQhiMEoqXwBggiIXG42MWxN058g1SVAitVqsTp1prYTtaVYy-2U9hX4WmZpduqi3qBIiVW5pvfcyyKpxWZV6yp3l6EKXpw83XwZk4ob6jg94rDSjA645Sw-7fOB8WjlC4IqVnL-cPigNU1pbwih8Xv92YMPuUI2fqhzCtTTtk6mzdl6MvTpFYmWrWyUKrgbAlpM65zGJFWZQcqo3WKaLUpOMgmvSovbZyc8cqU6xh660yZw0-IOpXawuBDsqkEr8sRPAxmND7PjhE1zo7WQ9ygEdwGqcfFgx5cMk9236Pi24fTryefZmfnH5cnx2czxQXNs74nVGvWAW87TSjTrKWCtKLXasGh4S0TumpEDcCrpiPakIaIjoqK1oaqRrOj4vmV7tqFJHdlTZLyFksnCGNILK8IHeBCrqMdMU4ZwMq_P0IcJESsiDOyrXlrWiIM7ZsFVwI6jEe3vK40rRa1Qq13u9umbjRaYf4R3J7ovsXblRzCpRSkqfmiQoHXO4EYfkwmZTnapIxz4E2YtnFjYzHtBUX0xT_o3dntqAEwAev7gPeqrag8bghtKL4IUvM7KHy0Ga3CGdy2et_hzZ4DMtn8ygNMKcnll8__z55_32df3mJXBlxe4fDh1OIc74OLK1DFkFI0_U2RKyK3K3RdDbldIblbIXR7drtBN07XO8P-AH9-GZY</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2686209033</pqid></control><display><type>article</type><title>Demonstrating the utility of Escherichia coli asymptomatic bacteriuria isolates' virulence profile towards diagnosis and management-A preliminary analysis</title><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Maniam, Lalitha ; Vellasamy, Kumutha Malar ; Jindal, Hassan Mahmood ; Narayanan, Vallikannu ; Danaee, Mahmoud ; Vadivelu, Jamuna ; Pallath, Vinod</creator><contributor>Algammal, Abdelazeem Mohamed</contributor><creatorcontrib>Maniam, Lalitha ; Vellasamy, Kumutha Malar ; Jindal, Hassan Mahmood ; Narayanan, Vallikannu ; Danaee, Mahmoud ; Vadivelu, Jamuna ; Pallath, Vinod ; Algammal, Abdelazeem Mohamed</creatorcontrib><description>Asymptomatic bacteriuria (ASB) caused by Escherichia coli (E. coli) is a significant condition associated with pregnancy and is considered as prognostic for the development of symptomatic urinary tract infection (UTI). However, treating all ASB increases the use of antibiotics and leads to the development of multidrug resistance (MDR). Therefore, this study aimed to identify the distribution of UPEC associated virulence genes and antibiotic susceptibility among phylogroups of E. coli isolated from ASB in pregnancy. Moreover, the gene expression of selected virulence genes was also compared among two E. coli isolates (with different pathogenic potential) to determine its pathogenicity. One hundred and sixty E. coli isolates from midstream urine samples of pregnant women with ASB were subjected to PCR-based detection for its phylogroups and virulence genes. The antibiotic susceptibility of isolated strains was determined by the disc diffusion method. Expression of the virulence genes were determined through microarray analysis and quantitative Real-Time PCR. The prevalence of ASB in this study was 16.1%. Within ASB isolates, the occurrence of phylogroup B2 was the highest, and isolates from this group harboured most of the virulence genes studied. Overall, the most identified virulence genes among all phylogroups in descending order were fimH, chuA, kpsMTII, usp, fyuA, hlyA, iroN, cnf, papC, sfa, ompT, and sat. In this study, higher resistance to antibiotics was observed for ampicillin (77.5%), amoxicillin-clavulanate (54.4%), trimethoprim-sulfamethoxazole (46.9%) and amikacin (43.8%) compared to the other tested antibiotics and 51.9% of the tested isolates were MDR. Furthermore, hierarchical clustering and gene expression analysis demonstrated extreme polarization of pathogenic potential of E. coli causing ASB in pregnancy necessitating the need for bacterial isolate focused approach towards treatment of ASB.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0267296</identifier><identifier>PMID: 35522610</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Amikacin ; Amoxicillin ; Ampicillin ; Analysis ; Antibiotics ; Antimicrobial agents ; Asymptomatic ; Bacteria ; Bacterial infections ; Bacteriuria ; Biology and Life Sciences ; Cluster analysis ; Clustering ; Diabetes ; Diagnosis ; Disease susceptibility ; DNA microarrays ; Drug resistance ; E coli ; Escherichia coli ; Evaluation ; Gene expression ; Genes ; Health care ; Medical diagnosis ; Medical research ; Medicine and Health Sciences ; Multidrug resistance ; Nosocomial infections ; Pathogenicity ; Pathogens ; Pregnancy ; Properties ; Research and Analysis Methods ; Sulfamethoxazole ; Trimethoprim ; Trimethoprim-sulfamethoxazole ; Urinary tract ; Urine ; Urogenital system ; Virulence ; Virulence (Microbiology) ; Womens health</subject><ispartof>PloS one, 2022-05, Vol.17 (5), p.e0267296-e0267296</ispartof><rights>COPYRIGHT 2022 Public Library of Science</rights><rights>2022 Maniam et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 Maniam et al 2022 Maniam et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-ff02dd3ba68bd023d3829089fdc46a76839d1795aa617b0de0709b29125e2c7d3</citedby><cites>FETCH-LOGICAL-c692t-ff02dd3ba68bd023d3829089fdc46a76839d1795aa617b0de0709b29125e2c7d3</cites><orcidid>0000-0001-6414-7552</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9075641/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9075641/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35522610$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Algammal, Abdelazeem Mohamed</contributor><creatorcontrib>Maniam, Lalitha</creatorcontrib><creatorcontrib>Vellasamy, Kumutha Malar</creatorcontrib><creatorcontrib>Jindal, Hassan Mahmood</creatorcontrib><creatorcontrib>Narayanan, Vallikannu</creatorcontrib><creatorcontrib>Danaee, Mahmoud</creatorcontrib><creatorcontrib>Vadivelu, Jamuna</creatorcontrib><creatorcontrib>Pallath, Vinod</creatorcontrib><title>Demonstrating the utility of Escherichia coli asymptomatic bacteriuria isolates' virulence profile towards diagnosis and management-A preliminary analysis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Asymptomatic bacteriuria (ASB) caused by Escherichia coli (E. coli) is a significant condition associated with pregnancy and is considered as prognostic for the development of symptomatic urinary tract infection (UTI). However, treating all ASB increases the use of antibiotics and leads to the development of multidrug resistance (MDR). Therefore, this study aimed to identify the distribution of UPEC associated virulence genes and antibiotic susceptibility among phylogroups of E. coli isolated from ASB in pregnancy. Moreover, the gene expression of selected virulence genes was also compared among two E. coli isolates (with different pathogenic potential) to determine its pathogenicity. One hundred and sixty E. coli isolates from midstream urine samples of pregnant women with ASB were subjected to PCR-based detection for its phylogroups and virulence genes. The antibiotic susceptibility of isolated strains was determined by the disc diffusion method. Expression of the virulence genes were determined through microarray analysis and quantitative Real-Time PCR. The prevalence of ASB in this study was 16.1%. Within ASB isolates, the occurrence of phylogroup B2 was the highest, and isolates from this group harboured most of the virulence genes studied. Overall, the most identified virulence genes among all phylogroups in descending order were fimH, chuA, kpsMTII, usp, fyuA, hlyA, iroN, cnf, papC, sfa, ompT, and sat. In this study, higher resistance to antibiotics was observed for ampicillin (77.5%), amoxicillin-clavulanate (54.4%), trimethoprim-sulfamethoxazole (46.9%) and amikacin (43.8%) compared to the other tested antibiotics and 51.9% of the tested isolates were MDR. Furthermore, hierarchical clustering and gene expression analysis demonstrated extreme polarization of pathogenic potential of E. coli causing ASB in pregnancy necessitating the need for bacterial isolate focused approach towards treatment of ASB.</description><subject>Amikacin</subject><subject>Amoxicillin</subject><subject>Ampicillin</subject><subject>Analysis</subject><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Asymptomatic</subject><subject>Bacteria</subject><subject>Bacterial infections</subject><subject>Bacteriuria</subject><subject>Biology and Life Sciences</subject><subject>Cluster analysis</subject><subject>Clustering</subject><subject>Diabetes</subject><subject>Diagnosis</subject><subject>Disease susceptibility</subject><subject>DNA microarrays</subject><subject>Drug resistance</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Evaluation</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Health care</subject><subject>Medical diagnosis</subject><subject>Medical research</subject><subject>Medicine and Health Sciences</subject><subject>Multidrug resistance</subject><subject>Nosocomial infections</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Pregnancy</subject><subject>Properties</subject><subject>Research and Analysis Methods</subject><subject>Sulfamethoxazole</subject><subject>Trimethoprim</subject><subject>Trimethoprim-sulfamethoxazole</subject><subject>Urinary tract</subject><subject>Urine</subject><subject>Urogenital system</subject><subject>Virulence</subject><subject>Virulence (Microbiology)</subject><subject>Womens health</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9tu1DAQhiMEoqXwBggiIXG42MWxN058g1SVAitVqsTp1prYTtaVYy-2U9hX4WmZpduqi3qBIiVW5pvfcyyKpxWZV6yp3l6EKXpw83XwZk4ob6jg94rDSjA645Sw-7fOB8WjlC4IqVnL-cPigNU1pbwih8Xv92YMPuUI2fqhzCtTTtk6mzdl6MvTpFYmWrWyUKrgbAlpM65zGJFWZQcqo3WKaLUpOMgmvSovbZyc8cqU6xh660yZw0-IOpXawuBDsqkEr8sRPAxmND7PjhE1zo7WQ9ygEdwGqcfFgx5cMk9236Pi24fTryefZmfnH5cnx2czxQXNs74nVGvWAW87TSjTrKWCtKLXasGh4S0TumpEDcCrpiPakIaIjoqK1oaqRrOj4vmV7tqFJHdlTZLyFksnCGNILK8IHeBCrqMdMU4ZwMq_P0IcJESsiDOyrXlrWiIM7ZsFVwI6jEe3vK40rRa1Qq13u9umbjRaYf4R3J7ovsXblRzCpRSkqfmiQoHXO4EYfkwmZTnapIxz4E2YtnFjYzHtBUX0xT_o3dntqAEwAev7gPeqrag8bghtKL4IUvM7KHy0Ga3CGdy2et_hzZ4DMtn8ygNMKcnll8__z55_32df3mJXBlxe4fDh1OIc74OLK1DFkFI0_U2RKyK3K3RdDbldIblbIXR7drtBN07XO8P-AH9-GZY</recordid><startdate>20220506</startdate><enddate>20220506</enddate><creator>Maniam, Lalitha</creator><creator>Vellasamy, Kumutha Malar</creator><creator>Jindal, Hassan Mahmood</creator><creator>Narayanan, Vallikannu</creator><creator>Danaee, Mahmoud</creator><creator>Vadivelu, Jamuna</creator><creator>Pallath, Vinod</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-6414-7552</orcidid></search><sort><creationdate>20220506</creationdate><title>Demonstrating the utility of Escherichia coli asymptomatic bacteriuria isolates' virulence profile towards diagnosis and management-A preliminary analysis</title><author>Maniam, Lalitha ; Vellasamy, Kumutha Malar ; Jindal, Hassan Mahmood ; Narayanan, Vallikannu ; Danaee, Mahmoud ; Vadivelu, Jamuna ; Pallath, Vinod</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-ff02dd3ba68bd023d3829089fdc46a76839d1795aa617b0de0709b29125e2c7d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Amikacin</topic><topic>Amoxicillin</topic><topic>Ampicillin</topic><topic>Analysis</topic><topic>Antibiotics</topic><topic>Antimicrobial agents</topic><topic>Asymptomatic</topic><topic>Bacteria</topic><topic>Bacterial infections</topic><topic>Bacteriuria</topic><topic>Biology and Life Sciences</topic><topic>Cluster analysis</topic><topic>Clustering</topic><topic>Diabetes</topic><topic>Diagnosis</topic><topic>Disease susceptibility</topic><topic>DNA microarrays</topic><topic>Drug resistance</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Evaluation</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Health care</topic><topic>Medical diagnosis</topic><topic>Medical research</topic><topic>Medicine and Health Sciences</topic><topic>Multidrug resistance</topic><topic>Nosocomial infections</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>Pregnancy</topic><topic>Properties</topic><topic>Research and Analysis Methods</topic><topic>Sulfamethoxazole</topic><topic>Trimethoprim</topic><topic>Trimethoprim-sulfamethoxazole</topic><topic>Urinary tract</topic><topic>Urine</topic><topic>Urogenital system</topic><topic>Virulence</topic><topic>Virulence (Microbiology)</topic><topic>Womens health</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maniam, Lalitha</creatorcontrib><creatorcontrib>Vellasamy, Kumutha Malar</creatorcontrib><creatorcontrib>Jindal, Hassan Mahmood</creatorcontrib><creatorcontrib>Narayanan, Vallikannu</creatorcontrib><creatorcontrib>Danaee, Mahmoud</creatorcontrib><creatorcontrib>Vadivelu, Jamuna</creatorcontrib><creatorcontrib>Pallath, Vinod</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maniam, Lalitha</au><au>Vellasamy, Kumutha Malar</au><au>Jindal, Hassan Mahmood</au><au>Narayanan, Vallikannu</au><au>Danaee, Mahmoud</au><au>Vadivelu, Jamuna</au><au>Pallath, Vinod</au><au>Algammal, Abdelazeem Mohamed</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Demonstrating the utility of Escherichia coli asymptomatic bacteriuria isolates' virulence profile towards diagnosis and management-A preliminary analysis</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2022-05-06</date><risdate>2022</risdate><volume>17</volume><issue>5</issue><spage>e0267296</spage><epage>e0267296</epage><pages>e0267296-e0267296</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Asymptomatic bacteriuria (ASB) caused by Escherichia coli (E. coli) is a significant condition associated with pregnancy and is considered as prognostic for the development of symptomatic urinary tract infection (UTI). However, treating all ASB increases the use of antibiotics and leads to the development of multidrug resistance (MDR). Therefore, this study aimed to identify the distribution of UPEC associated virulence genes and antibiotic susceptibility among phylogroups of E. coli isolated from ASB in pregnancy. Moreover, the gene expression of selected virulence genes was also compared among two E. coli isolates (with different pathogenic potential) to determine its pathogenicity. One hundred and sixty E. coli isolates from midstream urine samples of pregnant women with ASB were subjected to PCR-based detection for its phylogroups and virulence genes. The antibiotic susceptibility of isolated strains was determined by the disc diffusion method. Expression of the virulence genes were determined through microarray analysis and quantitative Real-Time PCR. The prevalence of ASB in this study was 16.1%. Within ASB isolates, the occurrence of phylogroup B2 was the highest, and isolates from this group harboured most of the virulence genes studied. Overall, the most identified virulence genes among all phylogroups in descending order were fimH, chuA, kpsMTII, usp, fyuA, hlyA, iroN, cnf, papC, sfa, ompT, and sat. In this study, higher resistance to antibiotics was observed for ampicillin (77.5%), amoxicillin-clavulanate (54.4%), trimethoprim-sulfamethoxazole (46.9%) and amikacin (43.8%) compared to the other tested antibiotics and 51.9% of the tested isolates were MDR. Furthermore, hierarchical clustering and gene expression analysis demonstrated extreme polarization of pathogenic potential of E. coli causing ASB in pregnancy necessitating the need for bacterial isolate focused approach towards treatment of ASB.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>35522610</pmid><doi>10.1371/journal.pone.0267296</doi><tpages>e0267296</tpages><orcidid>https://orcid.org/0000-0001-6414-7552</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2022-05, Vol.17 (5), p.e0267296-e0267296 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2686209033 |
| source | DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Amikacin Amoxicillin Ampicillin Analysis Antibiotics Antimicrobial agents Asymptomatic Bacteria Bacterial infections Bacteriuria Biology and Life Sciences Cluster analysis Clustering Diabetes Diagnosis Disease susceptibility DNA microarrays Drug resistance E coli Escherichia coli Evaluation Gene expression Genes Health care Medical diagnosis Medical research Medicine and Health Sciences Multidrug resistance Nosocomial infections Pathogenicity Pathogens Pregnancy Properties Research and Analysis Methods Sulfamethoxazole Trimethoprim Trimethoprim-sulfamethoxazole Urinary tract Urine Urogenital system Virulence Virulence (Microbiology) Womens health |
| title | Demonstrating the utility of Escherichia coli asymptomatic bacteriuria isolates' virulence profile towards diagnosis and management-A preliminary analysis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-21T09%3A58%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Demonstrating%20the%20utility%20of%20Escherichia%20coli%20asymptomatic%20bacteriuria%20isolates'%20virulence%20profile%20towards%20diagnosis%20and%20management-A%20preliminary%20analysis&rft.jtitle=PloS%20one&rft.au=Maniam,%20Lalitha&rft.date=2022-05-06&rft.volume=17&rft.issue=5&rft.spage=e0267296&rft.epage=e0267296&rft.pages=e0267296-e0267296&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0267296&rft_dat=%3Cgale_plos_%3EA702727020%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2686209033&rft_id=info:pmid/35522610&rft_galeid=A702727020&rft_doaj_id=oai_doaj_org_article_8568e809e2f746c9abd38d8651d2145c&rfr_iscdi=true |