Weed-infecting viruses in a tropical agroecosystem present different threats to crops and evolutionary histories
In the Caribbean Basin, malvaceous weeds commonly show striking golden/yellow mosaic symptoms. Leaf samples from Malachra sp. and Abutilon sp. plants with these symptoms were collected in Hispaniola from 2014 to 2020. PCR tests with degenerate primers revealed that all samples were infected with a b...
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| description | In the Caribbean Basin, malvaceous weeds commonly show striking golden/yellow mosaic symptoms. Leaf samples from Malachra sp. and Abutilon sp. plants with these symptoms were collected in Hispaniola from 2014 to 2020. PCR tests with degenerate primers revealed that all samples were infected with a bipartite begomovirus, and sequence analyses showed that Malachra sp. plants were infected with tobacco leaf curl Cuba virus (TbLCuCV), whereas the Abutilon sp. plants were infected with a new bipartite begomovirus, tentatively named Abutilon golden yellow mosaic virus (AbGYMV). Phylogenetic analyses showed that TbLCuCV and AbGYMV are distinct but closely related species, which are most closely related to bipartite begomoviruses infecting weeds in the Caribbean Basin. Infectious cloned DNA-A and DNA-B components were used to fulfilled Koch's postulates for these diseases of Malachra sp. and Abutilon sp. In host range studies, TbLCuCV also induced severe symptoms in Nicotiana benthamiana, tobacco and common bean plants; whereas AbGYMV induced few or no symptoms in plants of these species. Pseudorecombinants generated with the infectious clones of these viruses were highly infectious and induced severe symptoms in N. benthamiana and Malachra sp., and both viruses coinfected Malachra sp., and possibly facilitating virus evolution via recombination and pseudorecombination. Together, our results suggest that TbLCuCV primarily infects Malachra sp. in the Caribbean Basin, and occasionally spills over to infect and cause disease in crops; whereas AbGYMV is well-adapted to an Abutilon sp. in the Dominican Republic and has not been reported infecting crops. |
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Leaf samples from Malachra sp. and Abutilon sp. plants with these symptoms were collected in Hispaniola from 2014 to 2020. PCR tests with degenerate primers revealed that all samples were infected with a bipartite begomovirus, and sequence analyses showed that Malachra sp. plants were infected with tobacco leaf curl Cuba virus (TbLCuCV), whereas the Abutilon sp. plants were infected with a new bipartite begomovirus, tentatively named Abutilon golden yellow mosaic virus (AbGYMV). Phylogenetic analyses showed that TbLCuCV and AbGYMV are distinct but closely related species, which are most closely related to bipartite begomoviruses infecting weeds in the Caribbean Basin. Infectious cloned DNA-A and DNA-B components were used to fulfilled Koch's postulates for these diseases of Malachra sp. and Abutilon sp. In host range studies, TbLCuCV also induced severe symptoms in Nicotiana benthamiana, tobacco and common bean plants; whereas AbGYMV induced few or no symptoms in plants of these species. Pseudorecombinants generated with the infectious clones of these viruses were highly infectious and induced severe symptoms in N. benthamiana and Malachra sp., and both viruses coinfected Malachra sp., and possibly facilitating virus evolution via recombination and pseudorecombination. Together, our results suggest that TbLCuCV primarily infects Malachra sp. in the Caribbean Basin, and occasionally spills over to infect and cause disease in crops; whereas AbGYMV is well-adapted to an Abutilon sp. in the Dominican Republic and has not been reported infecting crops.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0250066</identifier><identifier>PMID: 33909644</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abutilon ; Agricultural ecosystems ; Basins ; Begomovirus ; Binding sites ; Biology and Life Sciences ; Cloning ; Computer and Information Sciences ; Crop diseases ; Crops ; Deoxyribonucleic acid ; Diseases and pests ; DNA ; Ecosystem ; Environmental aspects ; Enzymes ; Evolution ; Flowers & plants ; Gene expression ; Genome, Viral ; Genomes ; Host range ; Leaf-curl ; Leaves ; Malachra ; Natural history ; Nicotiana - virology ; Phaseolus - virology ; Phylogeny ; Plant diseases ; Plant Diseases - virology ; Plant pathology ; Plant viruses ; Plants ; Recombination ; Research and Analysis Methods ; Ribosomal DNA ; Risk factors ; RNA polymerase ; Signs and symptoms ; Tobacco ; Tropical crops ; Virus diseases of plants ; Viruses ; Weeds</subject><ispartof>PloS one, 2021-04, Vol.16 (4), p.e0250066-e0250066</ispartof><rights>COPYRIGHT 2021 Public Library of Science</rights><rights>2021 Maliano et al. 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Leaf samples from Malachra sp. and Abutilon sp. plants with these symptoms were collected in Hispaniola from 2014 to 2020. PCR tests with degenerate primers revealed that all samples were infected with a bipartite begomovirus, and sequence analyses showed that Malachra sp. plants were infected with tobacco leaf curl Cuba virus (TbLCuCV), whereas the Abutilon sp. plants were infected with a new bipartite begomovirus, tentatively named Abutilon golden yellow mosaic virus (AbGYMV). Phylogenetic analyses showed that TbLCuCV and AbGYMV are distinct but closely related species, which are most closely related to bipartite begomoviruses infecting weeds in the Caribbean Basin. Infectious cloned DNA-A and DNA-B components were used to fulfilled Koch's postulates for these diseases of Malachra sp. and Abutilon sp. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maliano, Minor R</au><au>Macedo, Mônica A</au><au>Rojas, Maria R</au><au>Gilbertson, Robert L</au><au>Pappu, Hanu R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Weed-infecting viruses in a tropical agroecosystem present different threats to crops and evolutionary histories</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2021-04-28</date><risdate>2021</risdate><volume>16</volume><issue>4</issue><spage>e0250066</spage><epage>e0250066</epage><pages>e0250066-e0250066</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>In the Caribbean Basin, malvaceous weeds commonly show striking golden/yellow mosaic symptoms. Leaf samples from Malachra sp. and Abutilon sp. plants with these symptoms were collected in Hispaniola from 2014 to 2020. PCR tests with degenerate primers revealed that all samples were infected with a bipartite begomovirus, and sequence analyses showed that Malachra sp. plants were infected with tobacco leaf curl Cuba virus (TbLCuCV), whereas the Abutilon sp. plants were infected with a new bipartite begomovirus, tentatively named Abutilon golden yellow mosaic virus (AbGYMV). Phylogenetic analyses showed that TbLCuCV and AbGYMV are distinct but closely related species, which are most closely related to bipartite begomoviruses infecting weeds in the Caribbean Basin. Infectious cloned DNA-A and DNA-B components were used to fulfilled Koch's postulates for these diseases of Malachra sp. and Abutilon sp. In host range studies, TbLCuCV also induced severe symptoms in Nicotiana benthamiana, tobacco and common bean plants; whereas AbGYMV induced few or no symptoms in plants of these species. Pseudorecombinants generated with the infectious clones of these viruses were highly infectious and induced severe symptoms in N. benthamiana and Malachra sp., and both viruses coinfected Malachra sp., and possibly facilitating virus evolution via recombination and pseudorecombination. Together, our results suggest that TbLCuCV primarily infects Malachra sp. in the Caribbean Basin, and occasionally spills over to infect and cause disease in crops; whereas AbGYMV is well-adapted to an Abutilon sp. in the Dominican Republic and has not been reported infecting crops.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>33909644</pmid><doi>10.1371/journal.pone.0250066</doi><orcidid>https://orcid.org/0000-0003-3950-5930</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Abutilon Agricultural ecosystems Basins Begomovirus Binding sites Biology and Life Sciences Cloning Computer and Information Sciences Crop diseases Crops Deoxyribonucleic acid Diseases and pests DNA Ecosystem Environmental aspects Enzymes Evolution Flowers & plants Gene expression Genome, Viral Genomes Host range Leaf-curl Leaves Malachra Natural history Nicotiana - virology Phaseolus - virology Phylogeny Plant diseases Plant Diseases - virology Plant pathology Plant viruses Plants Recombination Research and Analysis Methods Ribosomal DNA Risk factors RNA polymerase Signs and symptoms Tobacco Tropical crops Virus diseases of plants Viruses Weeds |
| title | Weed-infecting viruses in a tropical agroecosystem present different threats to crops and evolutionary histories |
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