Genetic diversity of Cryptosporidium spp. in non-human primates in rural and urban areas of Ethiopia
Non-Human Primates (NHPs) harbor Cryptosporidium genotypes that can infect humans and vice versa. NHPs Chlorocebus aethiops and Colobus guereza and humans have overlapping territories in some regions of Ethiopia, which may increase the risk of zoonotic transmission of Cryptosporidium. This cross-sec...
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| creator | Hailu, Ambachew W Degarege, Abraham Petros, Beyene Costa, Damien Ayene, Yonas Yimam Villier, Ven-Ceslas Mouhajir, Abdelmounaim Favennec, Loic Razakandrainibe, Romy Adamu, Haileeysus |
| description | Non-Human Primates (NHPs) harbor Cryptosporidium genotypes that can infect humans and vice versa. NHPs Chlorocebus aethiops and Colobus guereza and humans have overlapping territories in some regions of Ethiopia, which may increase the risk of zoonotic transmission of Cryptosporidium. This cross-sectional study examined the molecular prevalence and subtypes of Cryptosporidium spp. from 185 fecal samples of Chlorocebus aethiops and Colobus guereza in rural and urban areas in Ethiopia. Samples were tested for Cryptosporidium infection using nested polymerase chain reaction (PCR), and subtypes were determined by sequencing a fragment of the 60-kDa glycoprotein gene (gp60). Of the 185 samples, fifty-one (27.56%) tested positive for Cryptosporidium infection. The species detected were C. parvum (n = 34), C. hominis (n = 12), and C. cuniculus (n = 3). Mixed infection with C. parvum and C. hominis were detected in 2 samples. Four C. hominis family subtypes (Ia, Ib, Id, and Ie) and one C. parvum family subtype (IIa) were identified. C. hominis IaA20 (n = 7) and C. parvum IIaA17G1R1 (n = 6) were the most prevalent subtypes detected. These results confirm that Chlorocebus aethiops and Colobus guereza can be infected with diverse C. parvum and C. hominis subtypes that can also potentially infect humans. Additional studies could help to understand the role of NHPs in the zoonotic transmission of Cryptosporidium in Ethiopia. |
| doi_str_mv | 10.1371/journal.pone.0267103 |
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NHPs Chlorocebus aethiops and Colobus guereza and humans have overlapping territories in some regions of Ethiopia, which may increase the risk of zoonotic transmission of Cryptosporidium. This cross-sectional study examined the molecular prevalence and subtypes of Cryptosporidium spp. from 185 fecal samples of Chlorocebus aethiops and Colobus guereza in rural and urban areas in Ethiopia. Samples were tested for Cryptosporidium infection using nested polymerase chain reaction (PCR), and subtypes were determined by sequencing a fragment of the 60-kDa glycoprotein gene (gp60). Of the 185 samples, fifty-one (27.56%) tested positive for Cryptosporidium infection. The species detected were C. parvum (n = 34), C. hominis (n = 12), and C. cuniculus (n = 3). Mixed infection with C. parvum and C. hominis were detected in 2 samples. Four C. hominis family subtypes (Ia, Ib, Id, and Ie) and one C. parvum family subtype (IIa) were identified. C. hominis IaA20 (n = 7) and C. parvum IIaA17G1R1 (n = 6) were the most prevalent subtypes detected. These results confirm that Chlorocebus aethiops and Colobus guereza can be infected with diverse C. parvum and C. hominis subtypes that can also potentially infect humans. Additional studies could help to understand the role of NHPs in the zoonotic transmission of Cryptosporidium in Ethiopia.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0267103</identifier><identifier>PMID: 35421188</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animals ; Biological diversity ; Biology and Life Sciences ; Chlorocebus aethiops ; Colobus ; Colobus guereza ; Cross-Sectional Studies ; Cryptosporidiosis - epidemiology ; Cryptosporidium ; Cryptosporidium - genetics ; Cryptosporidium hominis ; Defecation ; Ethiopia - epidemiology ; Feces ; Genetic aspects ; Genetic diversity ; Genetic Variation ; Genotype ; Genotype & phenotype ; Genotypes ; Glycoproteins ; Infections ; Laboratories ; Medicine ; Medicine and Health Sciences ; Mixed infection ; Molecular biology ; People and Places ; Pharmacy ; Polymerase chain reaction ; Potassium ; Primates ; Public health ; Research and Analysis Methods ; Rural areas ; Statistical analysis ; Urban areas ; Zoonoses</subject><ispartof>PloS one, 2022-04, Vol.17 (4), p.e0267103-e0267103</ispartof><rights>COPYRIGHT 2022 Public Library of Science</rights><rights>2022 Hailu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 Hailu et al 2022 Hailu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c622t-83dda7bb7250bac25550d02bbbbca81dd24651673fdf358bc6cebee70a9384f83</citedby><cites>FETCH-LOGICAL-c622t-83dda7bb7250bac25550d02bbbbca81dd24651673fdf358bc6cebee70a9384f83</cites><orcidid>0000-0003-2958-5471</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9009656/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9009656/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35421188$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Rosenthal, Benjamin M.</contributor><creatorcontrib>Hailu, Ambachew W</creatorcontrib><creatorcontrib>Degarege, Abraham</creatorcontrib><creatorcontrib>Petros, Beyene</creatorcontrib><creatorcontrib>Costa, Damien</creatorcontrib><creatorcontrib>Ayene, Yonas Yimam</creatorcontrib><creatorcontrib>Villier, Ven-Ceslas</creatorcontrib><creatorcontrib>Mouhajir, Abdelmounaim</creatorcontrib><creatorcontrib>Favennec, Loic</creatorcontrib><creatorcontrib>Razakandrainibe, Romy</creatorcontrib><creatorcontrib>Adamu, Haileeysus</creatorcontrib><title>Genetic diversity of Cryptosporidium spp. in non-human primates in rural and urban areas of Ethiopia</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Non-Human Primates (NHPs) harbor Cryptosporidium genotypes that can infect humans and vice versa. NHPs Chlorocebus aethiops and Colobus guereza and humans have overlapping territories in some regions of Ethiopia, which may increase the risk of zoonotic transmission of Cryptosporidium. This cross-sectional study examined the molecular prevalence and subtypes of Cryptosporidium spp. from 185 fecal samples of Chlorocebus aethiops and Colobus guereza in rural and urban areas in Ethiopia. Samples were tested for Cryptosporidium infection using nested polymerase chain reaction (PCR), and subtypes were determined by sequencing a fragment of the 60-kDa glycoprotein gene (gp60). Of the 185 samples, fifty-one (27.56%) tested positive for Cryptosporidium infection. The species detected were C. parvum (n = 34), C. hominis (n = 12), and C. cuniculus (n = 3). Mixed infection with C. parvum and C. hominis were detected in 2 samples. Four C. hominis family subtypes (Ia, Ib, Id, and Ie) and one C. parvum family subtype (IIa) were identified. C. hominis IaA20 (n = 7) and C. parvum IIaA17G1R1 (n = 6) were the most prevalent subtypes detected. These results confirm that Chlorocebus aethiops and Colobus guereza can be infected with diverse C. parvum and C. hominis subtypes that can also potentially infect humans. Additional studies could help to understand the role of NHPs in the zoonotic transmission of Cryptosporidium in Ethiopia.</description><subject>Analysis</subject><subject>Animals</subject><subject>Biological diversity</subject><subject>Biology and Life Sciences</subject><subject>Chlorocebus aethiops</subject><subject>Colobus</subject><subject>Colobus guereza</subject><subject>Cross-Sectional Studies</subject><subject>Cryptosporidiosis - epidemiology</subject><subject>Cryptosporidium</subject><subject>Cryptosporidium - genetics</subject><subject>Cryptosporidium hominis</subject><subject>Defecation</subject><subject>Ethiopia - epidemiology</subject><subject>Feces</subject><subject>Genetic aspects</subject><subject>Genetic diversity</subject><subject>Genetic Variation</subject><subject>Genotype</subject><subject>Genotype & 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diversity of Cryptosporidium spp. in non-human primates in rural and urban areas of Ethiopia</title><author>Hailu, Ambachew W ; Degarege, Abraham ; Petros, Beyene ; Costa, Damien ; Ayene, Yonas Yimam ; Villier, Ven-Ceslas ; Mouhajir, Abdelmounaim ; Favennec, Loic ; Razakandrainibe, Romy ; Adamu, Haileeysus</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c622t-83dda7bb7250bac25550d02bbbbca81dd24651673fdf358bc6cebee70a9384f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>Biological diversity</topic><topic>Biology and Life Sciences</topic><topic>Chlorocebus aethiops</topic><topic>Colobus</topic><topic>Colobus guereza</topic><topic>Cross-Sectional Studies</topic><topic>Cryptosporidiosis - epidemiology</topic><topic>Cryptosporidium</topic><topic>Cryptosporidium - genetics</topic><topic>Cryptosporidium 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versa. NHPs Chlorocebus aethiops and Colobus guereza and humans have overlapping territories in some regions of Ethiopia, which may increase the risk of zoonotic transmission of Cryptosporidium. This cross-sectional study examined the molecular prevalence and subtypes of Cryptosporidium spp. from 185 fecal samples of Chlorocebus aethiops and Colobus guereza in rural and urban areas in Ethiopia. Samples were tested for Cryptosporidium infection using nested polymerase chain reaction (PCR), and subtypes were determined by sequencing a fragment of the 60-kDa glycoprotein gene (gp60). Of the 185 samples, fifty-one (27.56%) tested positive for Cryptosporidium infection. The species detected were C. parvum (n = 34), C. hominis (n = 12), and C. cuniculus (n = 3). Mixed infection with C. parvum and C. hominis were detected in 2 samples. Four C. hominis family subtypes (Ia, Ib, Id, and Ie) and one C. parvum family subtype (IIa) were identified. C. hominis IaA20 (n = 7) and C. parvum IIaA17G1R1 (n = 6) were the most prevalent subtypes detected. These results confirm that Chlorocebus aethiops and Colobus guereza can be infected with diverse C. parvum and C. hominis subtypes that can also potentially infect humans. Additional studies could help to understand the role of NHPs in the zoonotic transmission of Cryptosporidium in Ethiopia.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>35421188</pmid><doi>10.1371/journal.pone.0267103</doi><tpages>e0267103</tpages><orcidid>https://orcid.org/0000-0003-2958-5471</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2022-04, Vol.17 (4), p.e0267103-e0267103 |
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| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS) Journals Open Access; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis Animals Biological diversity Biology and Life Sciences Chlorocebus aethiops Colobus Colobus guereza Cross-Sectional Studies Cryptosporidiosis - epidemiology Cryptosporidium Cryptosporidium - genetics Cryptosporidium hominis Defecation Ethiopia - epidemiology Feces Genetic aspects Genetic diversity Genetic Variation Genotype Genotype & phenotype Genotypes Glycoproteins Infections Laboratories Medicine Medicine and Health Sciences Mixed infection Molecular biology People and Places Pharmacy Polymerase chain reaction Potassium Primates Public health Research and Analysis Methods Rural areas Statistical analysis Urban areas Zoonoses |
| title | Genetic diversity of Cryptosporidium spp. in non-human primates in rural and urban areas of Ethiopia |
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