Plasma DNA and deoxyribonuclease are associated with glucose metabolism in healthy mice
It is currently unknown why obesity leads in some patients to prediabetes and metabolic syndrome. Microinflammation potentially caused by extracellular DNA is supposed to be involved. The aim of this cross-sectional study in healthy mice was to analyze the association between plasma extracellular DN...
Gespeichert in:
Veröffentlicht in: | PloS one 2022-04, Vol.17 (4), p.e0265099-e0265099 |
---|---|
Hauptverfasser: | , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | e0265099 |
---|---|
container_issue | 4 |
container_start_page | e0265099 |
container_title | PloS one |
container_volume | 17 |
creator | Kmeťová, Katarína Čonka, Jozef Janko, Jakub Illés, Júlia Uličná, Oľga Celec, Peter |
description | It is currently unknown why obesity leads in some patients to prediabetes and metabolic syndrome. Microinflammation potentially caused by extracellular DNA is supposed to be involved. The aim of this cross-sectional study in healthy mice was to analyze the association between plasma extracellular DNA and glucose metabolism. Fasting glycemia and insulin were measured in healthy adult female mice that subsequently underwent an oral glucose tolerance test. Indices of glucose metabolism and insulin sensitivity were calculated. DNA was isolated from plasma and quantified fluorometrically. Deoxyribonuclease (DNase) activity of plasma was measured using the single radial enzyme diffusion method. Fasting glycemia correlated negatively with both, extracellular DNA and DNase (r = -0.44 and r = -0.32, respectively). DNase was associated positively with the incremental area under curve (r = 0.35), while extracellular DNA correlated negatively with total area under curve of glycemia during oral glucose tolerance test (r = -0.34). Measures of insulin sensitivity were found to be associated with neither extracellular DNA, nor DNase. The hypothesis of an association of low DNase with increased fasting glucose was partially proved. Surprisingly, low extracellular DNA is associated with higher fasting glucose and lower glucose tolerance in mice. As novel therapeutic targets for prediabetes and metabolic syndrome are highly needed, this study provides novel unexpected associations within the limitations of the focus on physiological variability as it was conducted on healthy mice. The causality of these associations should be proved in further interventional experiments. |
doi_str_mv | 10.1371/journal.pone.0265099 |
format | Article |
fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2647649477</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A699588971</galeid><doaj_id>oai_doaj_org_article_b7375bf504274659ac58b0c1ecba56e8</doaj_id><sourcerecordid>A699588971</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4869-ad286a796b172390a05570913e74ad1a4e7a4fed2cbfe7ba0de8627c5a6a71983</originalsourceid><addsrcrecordid>eNqNkltv1DAQhSMEoqXwDxBEQkLwsIuT-PpSaVVuK1UUcX20Js5k15UTL3EC3X-Pt5tWG9QHFEWx7O-cyYxPkjzNyDwrRPbm0g9dC26-8S3OSc4ZUepecpypIp_xnBT3D9ZHyaMQLglhheT8YXJUxAWjShwnPz87CA2kbz8tUmirtEJ_te1s6dvBOISAKXTxDcEbCz1W6R_br9OVG4yPZw32UHpnQ5PaNl0juH69TRtr8HHyoAYX8Mn4PUm-v3_37ezj7Pziw_JscT4zVHI1gyqXHITiZSbyQhEgjAmisgIFhSoDigJojVVuyhpFCaRCyXNhGERVpmRxkjzf-26cD3qcSdA5p4JTRYWIxHJPVB4u9aazDXRb7cHq6w3frTR0vY3d6lIUgpU1IzQXlDMFhsmSmAxNCYzjrtrpWG0oG6wMtn0HbmI6PWntWq_8by2VZIqyaPBqNOj8rwFDrxsbDDoHLfrh-r8l4ZTlPKIv_kHv7m6kVhAbsG3tY12zM9ULrhSTUoksUvM7qPhUGC8rBqi2cX8ieD0RRKbHq34FQwh6-fXL_7MXP6bsywN2H5jg3dBb34YpSPeg6XwIHda3Q86I3uX_Zhp6l3895j_Knh1e0K3oJvDFX9GIAD4</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2647649477</pqid></control><display><type>article</type><title>Plasma DNA and deoxyribonuclease are associated with glucose metabolism in healthy mice</title><source>Public Library of Science (PLoS) Journals Open Access</source><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Kmeťová, Katarína ; Čonka, Jozef ; Janko, Jakub ; Illés, Júlia ; Uličná, Oľga ; Celec, Peter</creator><contributor>Baek, Kwang-Hyun</contributor><creatorcontrib>Kmeťová, Katarína ; Čonka, Jozef ; Janko, Jakub ; Illés, Júlia ; Uličná, Oľga ; Celec, Peter ; Baek, Kwang-Hyun</creatorcontrib><description>It is currently unknown why obesity leads in some patients to prediabetes and metabolic syndrome. Microinflammation potentially caused by extracellular DNA is supposed to be involved. The aim of this cross-sectional study in healthy mice was to analyze the association between plasma extracellular DNA and glucose metabolism. Fasting glycemia and insulin were measured in healthy adult female mice that subsequently underwent an oral glucose tolerance test. Indices of glucose metabolism and insulin sensitivity were calculated. DNA was isolated from plasma and quantified fluorometrically. Deoxyribonuclease (DNase) activity of plasma was measured using the single radial enzyme diffusion method. Fasting glycemia correlated negatively with both, extracellular DNA and DNase (r = -0.44 and r = -0.32, respectively). DNase was associated positively with the incremental area under curve (r = 0.35), while extracellular DNA correlated negatively with total area under curve of glycemia during oral glucose tolerance test (r = -0.34). Measures of insulin sensitivity were found to be associated with neither extracellular DNA, nor DNase. The hypothesis of an association of low DNase with increased fasting glucose was partially proved. Surprisingly, low extracellular DNA is associated with higher fasting glucose and lower glucose tolerance in mice. As novel therapeutic targets for prediabetes and metabolic syndrome are highly needed, this study provides novel unexpected associations within the limitations of the focus on physiological variability as it was conducted on healthy mice. The causality of these associations should be proved in further interventional experiments.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0265099</identifier><identifier>PMID: 35385497</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animals ; Biology and life sciences ; Blood glucose ; Blood Glucose - metabolism ; Cholesterol ; Cross-Sectional Studies ; Deoxyribonuclease ; Deoxyribonucleases - blood ; Deoxyribonucleic acid ; Diffusion rate ; DNA ; DNA - blood ; Fasting ; Female ; Genetic aspects ; Glucose ; Glucose metabolism ; Glucose tolerance ; Insulin ; Insulin - metabolism ; Insulin resistance ; Insulin Resistance - physiology ; Laboratory testing ; Liver ; Medical screening ; Medicine ; Medicine and Health Sciences ; Metabolic disorders ; Metabolic Syndrome ; Mice ; Mitochondrial DNA ; Obesity ; Physical Sciences ; Physiological aspects ; Plasma ; Prediabetic State ; Research and Analysis Methods ; Sensitivity ; Statistical analysis ; Therapeutic targets ; Weight control</subject><ispartof>PloS one, 2022-04, Vol.17 (4), p.e0265099-e0265099</ispartof><rights>COPYRIGHT 2022 Public Library of Science</rights><rights>2022 Kmeťová et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 Kmeťová et al 2022 Kmeťová et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4869-ad286a796b172390a05570913e74ad1a4e7a4fed2cbfe7ba0de8627c5a6a71983</cites><orcidid>0000-0001-5883-3580 ; 0000-0002-2730-4587</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8985945/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8985945/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,866,887,2106,2932,23875,27933,27934,53800,53802</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35385497$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Baek, Kwang-Hyun</contributor><creatorcontrib>Kmeťová, Katarína</creatorcontrib><creatorcontrib>Čonka, Jozef</creatorcontrib><creatorcontrib>Janko, Jakub</creatorcontrib><creatorcontrib>Illés, Júlia</creatorcontrib><creatorcontrib>Uličná, Oľga</creatorcontrib><creatorcontrib>Celec, Peter</creatorcontrib><title>Plasma DNA and deoxyribonuclease are associated with glucose metabolism in healthy mice</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>It is currently unknown why obesity leads in some patients to prediabetes and metabolic syndrome. Microinflammation potentially caused by extracellular DNA is supposed to be involved. The aim of this cross-sectional study in healthy mice was to analyze the association between plasma extracellular DNA and glucose metabolism. Fasting glycemia and insulin were measured in healthy adult female mice that subsequently underwent an oral glucose tolerance test. Indices of glucose metabolism and insulin sensitivity were calculated. DNA was isolated from plasma and quantified fluorometrically. Deoxyribonuclease (DNase) activity of plasma was measured using the single radial enzyme diffusion method. Fasting glycemia correlated negatively with both, extracellular DNA and DNase (r = -0.44 and r = -0.32, respectively). DNase was associated positively with the incremental area under curve (r = 0.35), while extracellular DNA correlated negatively with total area under curve of glycemia during oral glucose tolerance test (r = -0.34). Measures of insulin sensitivity were found to be associated with neither extracellular DNA, nor DNase. The hypothesis of an association of low DNase with increased fasting glucose was partially proved. Surprisingly, low extracellular DNA is associated with higher fasting glucose and lower glucose tolerance in mice. As novel therapeutic targets for prediabetes and metabolic syndrome are highly needed, this study provides novel unexpected associations within the limitations of the focus on physiological variability as it was conducted on healthy mice. The causality of these associations should be proved in further interventional experiments.</description><subject>Analysis</subject><subject>Animals</subject><subject>Biology and life sciences</subject><subject>Blood glucose</subject><subject>Blood Glucose - metabolism</subject><subject>Cholesterol</subject><subject>Cross-Sectional Studies</subject><subject>Deoxyribonuclease</subject><subject>Deoxyribonucleases - blood</subject><subject>Deoxyribonucleic acid</subject><subject>Diffusion rate</subject><subject>DNA</subject><subject>DNA - blood</subject><subject>Fasting</subject><subject>Female</subject><subject>Genetic aspects</subject><subject>Glucose</subject><subject>Glucose metabolism</subject><subject>Glucose tolerance</subject><subject>Insulin</subject><subject>Insulin - metabolism</subject><subject>Insulin resistance</subject><subject>Insulin Resistance - physiology</subject><subject>Laboratory testing</subject><subject>Liver</subject><subject>Medical screening</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Metabolic disorders</subject><subject>Metabolic Syndrome</subject><subject>Mice</subject><subject>Mitochondrial DNA</subject><subject>Obesity</subject><subject>Physical Sciences</subject><subject>Physiological aspects</subject><subject>Plasma</subject><subject>Prediabetic State</subject><subject>Research and Analysis Methods</subject><subject>Sensitivity</subject><subject>Statistical analysis</subject><subject>Therapeutic targets</subject><subject>Weight control</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkltv1DAQhSMEoqXwDxBEQkLwsIuT-PpSaVVuK1UUcX20Js5k15UTL3EC3X-Pt5tWG9QHFEWx7O-cyYxPkjzNyDwrRPbm0g9dC26-8S3OSc4ZUepecpypIp_xnBT3D9ZHyaMQLglhheT8YXJUxAWjShwnPz87CA2kbz8tUmirtEJ_te1s6dvBOISAKXTxDcEbCz1W6R_br9OVG4yPZw32UHpnQ5PaNl0juH69TRtr8HHyoAYX8Mn4PUm-v3_37ezj7Pziw_JscT4zVHI1gyqXHITiZSbyQhEgjAmisgIFhSoDigJojVVuyhpFCaRCyXNhGERVpmRxkjzf-26cD3qcSdA5p4JTRYWIxHJPVB4u9aazDXRb7cHq6w3frTR0vY3d6lIUgpU1IzQXlDMFhsmSmAxNCYzjrtrpWG0oG6wMtn0HbmI6PWntWq_8by2VZIqyaPBqNOj8rwFDrxsbDDoHLfrh-r8l4ZTlPKIv_kHv7m6kVhAbsG3tY12zM9ULrhSTUoksUvM7qPhUGC8rBqi2cX8ieD0RRKbHq34FQwh6-fXL_7MXP6bsywN2H5jg3dBb34YpSPeg6XwIHda3Q86I3uX_Zhp6l3895j_Knh1e0K3oJvDFX9GIAD4</recordid><startdate>20220406</startdate><enddate>20220406</enddate><creator>Kmeťová, Katarína</creator><creator>Čonka, Jozef</creator><creator>Janko, Jakub</creator><creator>Illés, Júlia</creator><creator>Uličná, Oľga</creator><creator>Celec, Peter</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-5883-3580</orcidid><orcidid>https://orcid.org/0000-0002-2730-4587</orcidid></search><sort><creationdate>20220406</creationdate><title>Plasma DNA and deoxyribonuclease are associated with glucose metabolism in healthy mice</title><author>Kmeťová, Katarína ; Čonka, Jozef ; Janko, Jakub ; Illés, Júlia ; Uličná, Oľga ; Celec, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4869-ad286a796b172390a05570913e74ad1a4e7a4fed2cbfe7ba0de8627c5a6a71983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>Biology and life sciences</topic><topic>Blood glucose</topic><topic>Blood Glucose - metabolism</topic><topic>Cholesterol</topic><topic>Cross-Sectional Studies</topic><topic>Deoxyribonuclease</topic><topic>Deoxyribonucleases - blood</topic><topic>Deoxyribonucleic acid</topic><topic>Diffusion rate</topic><topic>DNA</topic><topic>DNA - blood</topic><topic>Fasting</topic><topic>Female</topic><topic>Genetic aspects</topic><topic>Glucose</topic><topic>Glucose metabolism</topic><topic>Glucose tolerance</topic><topic>Insulin</topic><topic>Insulin - metabolism</topic><topic>Insulin resistance</topic><topic>Insulin Resistance - physiology</topic><topic>Laboratory testing</topic><topic>Liver</topic><topic>Medical screening</topic><topic>Medicine</topic><topic>Medicine and Health Sciences</topic><topic>Metabolic disorders</topic><topic>Metabolic Syndrome</topic><topic>Mice</topic><topic>Mitochondrial DNA</topic><topic>Obesity</topic><topic>Physical Sciences</topic><topic>Physiological aspects</topic><topic>Plasma</topic><topic>Prediabetic State</topic><topic>Research and Analysis Methods</topic><topic>Sensitivity</topic><topic>Statistical analysis</topic><topic>Therapeutic targets</topic><topic>Weight control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kmeťová, Katarína</creatorcontrib><creatorcontrib>Čonka, Jozef</creatorcontrib><creatorcontrib>Janko, Jakub</creatorcontrib><creatorcontrib>Illés, Júlia</creatorcontrib><creatorcontrib>Uličná, Oľga</creatorcontrib><creatorcontrib>Celec, Peter</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kmeťová, Katarína</au><au>Čonka, Jozef</au><au>Janko, Jakub</au><au>Illés, Júlia</au><au>Uličná, Oľga</au><au>Celec, Peter</au><au>Baek, Kwang-Hyun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Plasma DNA and deoxyribonuclease are associated with glucose metabolism in healthy mice</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2022-04-06</date><risdate>2022</risdate><volume>17</volume><issue>4</issue><spage>e0265099</spage><epage>e0265099</epage><pages>e0265099-e0265099</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>It is currently unknown why obesity leads in some patients to prediabetes and metabolic syndrome. Microinflammation potentially caused by extracellular DNA is supposed to be involved. The aim of this cross-sectional study in healthy mice was to analyze the association between plasma extracellular DNA and glucose metabolism. Fasting glycemia and insulin were measured in healthy adult female mice that subsequently underwent an oral glucose tolerance test. Indices of glucose metabolism and insulin sensitivity were calculated. DNA was isolated from plasma and quantified fluorometrically. Deoxyribonuclease (DNase) activity of plasma was measured using the single radial enzyme diffusion method. Fasting glycemia correlated negatively with both, extracellular DNA and DNase (r = -0.44 and r = -0.32, respectively). DNase was associated positively with the incremental area under curve (r = 0.35), while extracellular DNA correlated negatively with total area under curve of glycemia during oral glucose tolerance test (r = -0.34). Measures of insulin sensitivity were found to be associated with neither extracellular DNA, nor DNase. The hypothesis of an association of low DNase with increased fasting glucose was partially proved. Surprisingly, low extracellular DNA is associated with higher fasting glucose and lower glucose tolerance in mice. As novel therapeutic targets for prediabetes and metabolic syndrome are highly needed, this study provides novel unexpected associations within the limitations of the focus on physiological variability as it was conducted on healthy mice. The causality of these associations should be proved in further interventional experiments.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>35385497</pmid><doi>10.1371/journal.pone.0265099</doi><tpages>e0265099</tpages><orcidid>https://orcid.org/0000-0001-5883-3580</orcidid><orcidid>https://orcid.org/0000-0002-2730-4587</orcidid><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1932-6203 |
ispartof | PloS one, 2022-04, Vol.17 (4), p.e0265099-e0265099 |
issn | 1932-6203 1932-6203 |
language | eng |
recordid | cdi_plos_journals_2647649477 |
source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
subjects | Analysis Animals Biology and life sciences Blood glucose Blood Glucose - metabolism Cholesterol Cross-Sectional Studies Deoxyribonuclease Deoxyribonucleases - blood Deoxyribonucleic acid Diffusion rate DNA DNA - blood Fasting Female Genetic aspects Glucose Glucose metabolism Glucose tolerance Insulin Insulin - metabolism Insulin resistance Insulin Resistance - physiology Laboratory testing Liver Medical screening Medicine Medicine and Health Sciences Metabolic disorders Metabolic Syndrome Mice Mitochondrial DNA Obesity Physical Sciences Physiological aspects Plasma Prediabetic State Research and Analysis Methods Sensitivity Statistical analysis Therapeutic targets Weight control |
title | Plasma DNA and deoxyribonuclease are associated with glucose metabolism in healthy mice |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-01T07%3A30%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Plasma%20DNA%20and%20deoxyribonuclease%20are%20associated%20with%20glucose%20metabolism%20in%20healthy%20mice&rft.jtitle=PloS%20one&rft.au=Kme%C5%A5ov%C3%A1,%20Katar%C3%ADna&rft.date=2022-04-06&rft.volume=17&rft.issue=4&rft.spage=e0265099&rft.epage=e0265099&rft.pages=e0265099-e0265099&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0265099&rft_dat=%3Cgale_plos_%3EA699588971%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2647649477&rft_id=info:pmid/35385497&rft_galeid=A699588971&rft_doaj_id=oai_doaj_org_article_b7375bf504274659ac58b0c1ecba56e8&rfr_iscdi=true |