Whole genome sequencing of Neisseria meningitidis Y isolates collected in the Czech Republic in 1993-2018
The study presents the analysis of whole genome sequencing (WGS) data for Neisseria meningitidis serogroup Y isolates collected in the Czech Republic and their comparison to other countries. The aim of the study was to determine whether there are lineages of N. meningitidis serogroup Y in the Czech...
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| description | The study presents the analysis of whole genome sequencing (WGS) data for Neisseria meningitidis serogroup Y isolates collected in the Czech Republic and their comparison to other countries. The aim of the study was to determine whether there are lineages of N. meningitidis serogroup Y in the Czech Republic genetically related to foreign ones that have been causing an increase of the morbidity and the mortality of invasive meningococcal disease (IMD) world-wide recently.
The WGS data of 43 Czech N. meningitidis Y isolates, 35 from IMD and 8 from healthy carriers were analysed. Due to the potential of meningococcal B vaccines to induce protection against non-B serogroups, the coverage of Czech isolates of N. meningitidis Y by these vaccines was studied. The WGS data of Czech, European and non-European isolates of N. meningitidis serogroup Y were compared.
WGS assigned 36 isolates of N. meningitidis Y to five clonal complexes: cc23, cc92, cc167, cc103, and cc174, while seven isolates remained unassigned to any clonal complexes (ccUA). Eighteen invasive isolates belonged to clonal complex cc23, which was detected throughout the studied years. The occurrence of cc23 was recorded in all age groups of IMD patients, with the highest found in those aged 15-19 years. On the phylogenetic network isolates of cc23 form a separate lineage, distinct from all other isolates of N. meningitidis Y. The remaining isolates were assigned to other clonal complexes and have very low relatedness to cc23 isolates and to each other. The comparison with foreign WGS data showed that within the main genetic lineages, which are defined by clonal complexes, Czech isolates of N. meningitidis Y, similar to European ones, mostly cluster together and form geographical sublineages.
WGS analysis showed the population of Czech N. meningitidis Y isolates as relatively heterogeneous, containing a large number of genetic lineages. The Czech isolates of N. meningitidis Y follow the trend observed for European isolates. Our result was one of the bases for updating the recommended vaccination strategy in the Czech Republic. |
| doi_str_mv | 10.1371/journal.pone.0265066 |
| format | Article |
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The WGS data of 43 Czech N. meningitidis Y isolates, 35 from IMD and 8 from healthy carriers were analysed. Due to the potential of meningococcal B vaccines to induce protection against non-B serogroups, the coverage of Czech isolates of N. meningitidis Y by these vaccines was studied. The WGS data of Czech, European and non-European isolates of N. meningitidis serogroup Y were compared.
WGS assigned 36 isolates of N. meningitidis Y to five clonal complexes: cc23, cc92, cc167, cc103, and cc174, while seven isolates remained unassigned to any clonal complexes (ccUA). Eighteen invasive isolates belonged to clonal complex cc23, which was detected throughout the studied years. The occurrence of cc23 was recorded in all age groups of IMD patients, with the highest found in those aged 15-19 years. On the phylogenetic network isolates of cc23 form a separate lineage, distinct from all other isolates of N. meningitidis Y. The remaining isolates were assigned to other clonal complexes and have very low relatedness to cc23 isolates and to each other. The comparison with foreign WGS data showed that within the main genetic lineages, which are defined by clonal complexes, Czech isolates of N. meningitidis Y, similar to European ones, mostly cluster together and form geographical sublineages.
WGS analysis showed the population of Czech N. meningitidis Y isolates as relatively heterogeneous, containing a large number of genetic lineages. The Czech isolates of N. meningitidis Y follow the trend observed for European isolates. Our result was one of the bases for updating the recommended vaccination strategy in the Czech Republic.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0265066</identifier><identifier>PMID: 35271677</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Antigens ; Biology and Life Sciences ; Computer and Information Sciences ; Czech Republic - epidemiology ; DNA sequencing ; Epidemiology ; Fatalities ; Gene sequencing ; Genetic aspects ; Genomes ; Genomics ; Health care ; Health surveillance ; Humans ; Infections ; Invasive meningococcal disease ; Laboratories ; Medicine and Health Sciences ; Meningitis ; Meningococcal disease ; Meningococcal Infections - epidemiology ; Meningococcal Vaccines ; Microbiology ; Morbidity ; Neisseria meningitidis ; Nucleotide sequencing ; Older people ; People and places ; Phylogeny ; Population ; Public health ; Serogroup ; Vaccines ; Whole Genome Sequencing</subject><ispartof>PloS one, 2022-03, Vol.17 (3), p.e0265066-e0265066</ispartof><rights>COPYRIGHT 2022 Public Library of Science</rights><rights>2022 Honskus et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 Honskus et al 2022 Honskus et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-b64ea4d30ba511d0d10ec0f48ad363f36c2e21c637b79207146da7ea7dce94293</citedby><cites>FETCH-LOGICAL-c692t-b64ea4d30ba511d0d10ec0f48ad363f36c2e21c637b79207146da7ea7dce94293</cites><orcidid>0000-0002-3993-2994</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912901/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912901/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35271677$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Lin, Baochuan</contributor><creatorcontrib>Honskus, Michal</creatorcontrib><creatorcontrib>Okonji, Zuzana</creatorcontrib><creatorcontrib>Musilek, Martin</creatorcontrib><creatorcontrib>Krizova, Pavla</creatorcontrib><title>Whole genome sequencing of Neisseria meningitidis Y isolates collected in the Czech Republic in 1993-2018</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The study presents the analysis of whole genome sequencing (WGS) data for Neisseria meningitidis serogroup Y isolates collected in the Czech Republic and their comparison to other countries. The aim of the study was to determine whether there are lineages of N. meningitidis serogroup Y in the Czech Republic genetically related to foreign ones that have been causing an increase of the morbidity and the mortality of invasive meningococcal disease (IMD) world-wide recently.
The WGS data of 43 Czech N. meningitidis Y isolates, 35 from IMD and 8 from healthy carriers were analysed. Due to the potential of meningococcal B vaccines to induce protection against non-B serogroups, the coverage of Czech isolates of N. meningitidis Y by these vaccines was studied. The WGS data of Czech, European and non-European isolates of N. meningitidis serogroup Y were compared.
WGS assigned 36 isolates of N. meningitidis Y to five clonal complexes: cc23, cc92, cc167, cc103, and cc174, while seven isolates remained unassigned to any clonal complexes (ccUA). Eighteen invasive isolates belonged to clonal complex cc23, which was detected throughout the studied years. The occurrence of cc23 was recorded in all age groups of IMD patients, with the highest found in those aged 15-19 years. On the phylogenetic network isolates of cc23 form a separate lineage, distinct from all other isolates of N. meningitidis Y. The remaining isolates were assigned to other clonal complexes and have very low relatedness to cc23 isolates and to each other. The comparison with foreign WGS data showed that within the main genetic lineages, which are defined by clonal complexes, Czech isolates of N. meningitidis Y, similar to European ones, mostly cluster together and form geographical sublineages.
WGS analysis showed the population of Czech N. meningitidis Y isolates as relatively heterogeneous, containing a large number of genetic lineages. The Czech isolates of N. meningitidis Y follow the trend observed for European isolates. Our result was one of the bases for updating the recommended vaccination strategy in the Czech Republic.</description><subject>Analysis</subject><subject>Antigens</subject><subject>Biology and Life Sciences</subject><subject>Computer and Information Sciences</subject><subject>Czech Republic - epidemiology</subject><subject>DNA sequencing</subject><subject>Epidemiology</subject><subject>Fatalities</subject><subject>Gene sequencing</subject><subject>Genetic aspects</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Health care</subject><subject>Health surveillance</subject><subject>Humans</subject><subject>Infections</subject><subject>Invasive meningococcal disease</subject><subject>Laboratories</subject><subject>Medicine and Health Sciences</subject><subject>Meningitis</subject><subject>Meningococcal disease</subject><subject>Meningococcal Infections - epidemiology</subject><subject>Meningococcal Vaccines</subject><subject>Microbiology</subject><subject>Morbidity</subject><subject>Neisseria meningitidis</subject><subject>Nucleotide sequencing</subject><subject>Older people</subject><subject>People and places</subject><subject>Phylogeny</subject><subject>Population</subject><subject>Public health</subject><subject>Serogroup</subject><subject>Vaccines</subject><subject>Whole Genome Sequencing</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12L1DAUhoso7rr6D0QLgujFjPlok-ZGWAY_BhYX1i-8Cml62mbIJGOTivrrzTjdZSp7YXqRcvKcNydvcrLsMUZLTDl-tfHj4JRd7ryDJSKsRIzdyU6xoGTBCKJ3j_5PsgchbBAqacXY_eyEloRjxvlpZr723kLegfNbyAN8H8Fp47rct_kHMCHAYFS-BZdiJprGhPxbboK3KkLItbcWdIQmNy6PPeSr36D7_Ap2Y22N3kexEHRBEK4eZvdaZQM8muaz7PPbN59W7xcXl-_Wq_OLhWaCxEXNClBFQ1GtSowb1GAEGrVFpRrKaEuZJkCwZpTXXBDEccEaxUHxRoMoiKBn2dOD7s76ICeXgiSMVigNQROxPhCNVxu5G8xWDb-kV0b-Dfihk2qIRluQxd5XzlHL66KolVYt1C2qRKqiZaJkSev1tNtYbyHV4OKg7Ex0vuJMLzv_Q1YCE4FwEngxCQw-mR-i3JqgwVrlwI-HujkhoiwS-uwf9PbTTVSn0gGMa33aV-9F5TkTjFIqqjJRy1uo9DWwNTo9qdak-Czh5SwhMRF-xk6NIcj1x6v_Zy-_zNnnR2wPysY-Pa8xGu_CHCwOoB58CAO0NyZjJPcdce2G3HeEnDoipT05vqCbpOsWoH8AzDcEpw</recordid><startdate>20220310</startdate><enddate>20220310</enddate><creator>Honskus, Michal</creator><creator>Okonji, Zuzana</creator><creator>Musilek, Martin</creator><creator>Krizova, Pavla</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-3993-2994</orcidid></search><sort><creationdate>20220310</creationdate><title>Whole genome sequencing of Neisseria meningitidis Y isolates collected in the Czech Republic in 1993-2018</title><author>Honskus, Michal ; Okonji, Zuzana ; Musilek, Martin ; Krizova, Pavla</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-b64ea4d30ba511d0d10ec0f48ad363f36c2e21c637b79207146da7ea7dce94293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Analysis</topic><topic>Antigens</topic><topic>Biology and Life Sciences</topic><topic>Computer and Information Sciences</topic><topic>Czech Republic - epidemiology</topic><topic>DNA sequencing</topic><topic>Epidemiology</topic><topic>Fatalities</topic><topic>Gene sequencing</topic><topic>Genetic aspects</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Health care</topic><topic>Health surveillance</topic><topic>Humans</topic><topic>Infections</topic><topic>Invasive meningococcal disease</topic><topic>Laboratories</topic><topic>Medicine and Health Sciences</topic><topic>Meningitis</topic><topic>Meningococcal disease</topic><topic>Meningococcal Infections - epidemiology</topic><topic>Meningococcal Vaccines</topic><topic>Microbiology</topic><topic>Morbidity</topic><topic>Neisseria meningitidis</topic><topic>Nucleotide sequencing</topic><topic>Older people</topic><topic>People and places</topic><topic>Phylogeny</topic><topic>Population</topic><topic>Public health</topic><topic>Serogroup</topic><topic>Vaccines</topic><topic>Whole Genome Sequencing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Honskus, Michal</creatorcontrib><creatorcontrib>Okonji, Zuzana</creatorcontrib><creatorcontrib>Musilek, Martin</creatorcontrib><creatorcontrib>Krizova, Pavla</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Honskus, Michal</au><au>Okonji, Zuzana</au><au>Musilek, Martin</au><au>Krizova, Pavla</au><au>Lin, Baochuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Whole genome sequencing of Neisseria meningitidis Y isolates collected in the Czech Republic in 1993-2018</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2022-03-10</date><risdate>2022</risdate><volume>17</volume><issue>3</issue><spage>e0265066</spage><epage>e0265066</epage><pages>e0265066-e0265066</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The study presents the analysis of whole genome sequencing (WGS) data for Neisseria meningitidis serogroup Y isolates collected in the Czech Republic and their comparison to other countries. The aim of the study was to determine whether there are lineages of N. meningitidis serogroup Y in the Czech Republic genetically related to foreign ones that have been causing an increase of the morbidity and the mortality of invasive meningococcal disease (IMD) world-wide recently.
The WGS data of 43 Czech N. meningitidis Y isolates, 35 from IMD and 8 from healthy carriers were analysed. Due to the potential of meningococcal B vaccines to induce protection against non-B serogroups, the coverage of Czech isolates of N. meningitidis Y by these vaccines was studied. The WGS data of Czech, European and non-European isolates of N. meningitidis serogroup Y were compared.
WGS assigned 36 isolates of N. meningitidis Y to five clonal complexes: cc23, cc92, cc167, cc103, and cc174, while seven isolates remained unassigned to any clonal complexes (ccUA). Eighteen invasive isolates belonged to clonal complex cc23, which was detected throughout the studied years. The occurrence of cc23 was recorded in all age groups of IMD patients, with the highest found in those aged 15-19 years. On the phylogenetic network isolates of cc23 form a separate lineage, distinct from all other isolates of N. meningitidis Y. The remaining isolates were assigned to other clonal complexes and have very low relatedness to cc23 isolates and to each other. The comparison with foreign WGS data showed that within the main genetic lineages, which are defined by clonal complexes, Czech isolates of N. meningitidis Y, similar to European ones, mostly cluster together and form geographical sublineages.
WGS analysis showed the population of Czech N. meningitidis Y isolates as relatively heterogeneous, containing a large number of genetic lineages. The Czech isolates of N. meningitidis Y follow the trend observed for European isolates. Our result was one of the bases for updating the recommended vaccination strategy in the Czech Republic.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>35271677</pmid><doi>10.1371/journal.pone.0265066</doi><tpages>e0265066</tpages><orcidid>https://orcid.org/0000-0002-3993-2994</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Analysis Antigens Biology and Life Sciences Computer and Information Sciences Czech Republic - epidemiology DNA sequencing Epidemiology Fatalities Gene sequencing Genetic aspects Genomes Genomics Health care Health surveillance Humans Infections Invasive meningococcal disease Laboratories Medicine and Health Sciences Meningitis Meningococcal disease Meningococcal Infections - epidemiology Meningococcal Vaccines Microbiology Morbidity Neisseria meningitidis Nucleotide sequencing Older people People and places Phylogeny Population Public health Serogroup Vaccines Whole Genome Sequencing |
| title | Whole genome sequencing of Neisseria meningitidis Y isolates collected in the Czech Republic in 1993-2018 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T18%3A23%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Whole%20genome%20sequencing%20of%20Neisseria%20meningitidis%20Y%20isolates%20collected%20in%20the%20Czech%20Republic%20in%201993-2018&rft.jtitle=PloS%20one&rft.au=Honskus,%20Michal&rft.date=2022-03-10&rft.volume=17&rft.issue=3&rft.spage=e0265066&rft.epage=e0265066&rft.pages=e0265066-e0265066&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0265066&rft_dat=%3Cgale_plos_%3EA696333985%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2638000093&rft_id=info:pmid/35271677&rft_galeid=A696333985&rft_doaj_id=oai_doaj_org_article_42650770f7b44bacafebf0896c2f6956&rfr_iscdi=true |