Photobiomodulation reduces hippocampal apoptotic cell death and produces a Raman spectroscopic "signature"
Apoptotic cell death within the brain represents a significant contributing factor to impaired post-traumatic tissue function and poor clinical outcome after traumatic brain injury. After irradiation with light in the wavelength range of 600-1200 nm (photobiomodulation), previous investigations have...
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| Veröffentlicht in: | PloS one 2022-03, Vol.17 (3), p.e0264533-e0264533 |
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| creator | Davies, David J Hadis, Mohammed Di Pietro, Valentina Lazzarino, Giuseppe Forcione, Mario Harris, Georgia Stevens, Andrew R Soon, Wai Cheong Goldberg Oppenheimer, Pola Milward, Michael Belli, Antonio Palin, William M |
| description | Apoptotic cell death within the brain represents a significant contributing factor to impaired post-traumatic tissue function and poor clinical outcome after traumatic brain injury. After irradiation with light in the wavelength range of 600-1200 nm (photobiomodulation), previous investigations have reported a reduction in apoptosis in various tissues. This study investigates the effect of 660 nm photobiomodulation on organotypic slice cultured hippocampal tissue of rats, examining the effect on apoptotic cell loss. Tissue optical Raman spectroscopic changes were evaluated. A significantly higher proportion of apoptotic cells 62.8±12.2% vs 48.6±13.7% (P |
| doi_str_mv | 10.1371/journal.pone.0264533 |
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After irradiation with light in the wavelength range of 600-1200 nm (photobiomodulation), previous investigations have reported a reduction in apoptosis in various tissues. This study investigates the effect of 660 nm photobiomodulation on organotypic slice cultured hippocampal tissue of rats, examining the effect on apoptotic cell loss. Tissue optical Raman spectroscopic changes were evaluated. A significantly higher proportion of apoptotic cells 62.8±12.2% vs 48.6±13.7% (P<0.0001) per region were observed in the control group compared with the photobiomodulation group. After photobiomodulation, Raman spectroscopic observations demonstrated 1440/1660 cm-1 spectral shift. Photobiomodulation has the potential for therapeutic utility, reducing cell loss to apoptosis in injured neurological tissue, as demonstrated in this in vitro model. A clear Raman spectroscopic signal was observed after apparent optimal irradiation, potentially integrable into therapeutic light delivery apparatus for real-time dose metering.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0264533</identifier><identifier>PMID: 35239693</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aging ; Animal tissues ; Animals ; Apoptosis ; Biochemistry ; Bioengineering ; Biology and Life Sciences ; Brain ; Brain Injuries, Traumatic - metabolism ; Brain research ; Cell death ; Contusions ; Dentistry ; Fibroblasts ; Head injuries ; Health aspects ; Hippocampus ; Hippocampus - metabolism ; Hospitals ; Inflammation ; Injuries ; Injury prevention ; Irradiation ; Light ; Light therapy ; Low-Level Light Therapy - methods ; Medical research ; Medicine and Health Sciences ; Methods ; Neurosciences ; Ophthalmology ; Photobiology ; Physical Sciences ; Radiation ; Raman spectroscopy ; Rats ; Research and Analysis Methods ; Spectroscopic observation ; Spectrum Analysis, Raman ; Traumatic brain injury ; Wound healing</subject><ispartof>PloS one, 2022-03, Vol.17 (3), p.e0264533-e0264533</ispartof><rights>COPYRIGHT 2022 Public Library of Science</rights><rights>2022 Davies et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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After irradiation with light in the wavelength range of 600-1200 nm (photobiomodulation), previous investigations have reported a reduction in apoptosis in various tissues. This study investigates the effect of 660 nm photobiomodulation on organotypic slice cultured hippocampal tissue of rats, examining the effect on apoptotic cell loss. Tissue optical Raman spectroscopic changes were evaluated. A significantly higher proportion of apoptotic cells 62.8±12.2% vs 48.6±13.7% (P<0.0001) per region were observed in the control group compared with the photobiomodulation group. After photobiomodulation, Raman spectroscopic observations demonstrated 1440/1660 cm-1 spectral shift. Photobiomodulation has the potential for therapeutic utility, reducing cell loss to apoptosis in injured neurological tissue, as demonstrated in this in vitro model. A clear Raman spectroscopic signal was observed after apparent optimal irradiation, potentially integrable into therapeutic light delivery apparatus for real-time dose metering.</description><subject>Aging</subject><subject>Animal tissues</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Biochemistry</subject><subject>Bioengineering</subject><subject>Biology and Life Sciences</subject><subject>Brain</subject><subject>Brain Injuries, Traumatic - metabolism</subject><subject>Brain research</subject><subject>Cell death</subject><subject>Contusions</subject><subject>Dentistry</subject><subject>Fibroblasts</subject><subject>Head injuries</subject><subject>Health aspects</subject><subject>Hippocampus</subject><subject>Hippocampus - metabolism</subject><subject>Hospitals</subject><subject>Inflammation</subject><subject>Injuries</subject><subject>Injury prevention</subject><subject>Irradiation</subject><subject>Light</subject><subject>Light therapy</subject><subject>Low-Level Light Therapy - methods</subject><subject>Medical research</subject><subject>Medicine and Health Sciences</subject><subject>Methods</subject><subject>Neurosciences</subject><subject>Ophthalmology</subject><subject>Photobiology</subject><subject>Physical Sciences</subject><subject>Radiation</subject><subject>Raman spectroscopy</subject><subject>Rats</subject><subject>Research and Analysis Methods</subject><subject>Spectroscopic observation</subject><subject>Spectrum Analysis, Raman</subject><subject>Traumatic brain injury</subject><subject>Wound healing</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12L1DAUhoso7jr6D0TLCqIXM6ZNkyY3wrL4MbCwsn7chtMknWZom5qkov_edKe7TGUvpBcpyXPenPPmnCR5nqFNhsvs3d6Orod2M9heb1BOC4Lxg-Q04zhf0xzhh0f_J8kT7_cIEcwofZycYJJjTjk-TfZfGhtsZWxn1dhCMLZPnVaj1D5tzDBYCd0AbQqDHYINRqZSt22qNIQmhV6lg7MHGtJr6KBP_aBlcNZLO0T6zJtdD2F0-uxp8qiG1utn87pKvn_88O3i8_ry6tP24vxyLSnPw7rCXCIFkkimEEcZobqiiuESYYmqSiPJi0rVWFOUsbqGWipGNadEKalJRfAqeXnQHVrrxWyTFznFpGAMIxaJ7YFQFvZicKYD90dYMOJmw7qdABdrbbXIqCpLWhESKy5UCVWmNaOEFCWnNYvWrpL3821j1emYQh8ctAvR5UlvGrGzvwRjHFOGo8CbWcDZn6P2QXTGTyZDr-14kzfNCkI4jeirf9D7q5upHcQCTF_beK-cRMU55ZMQK6a8N_dQ8VO6MzL2VG3i_iLg7SIgMkH_DjsYvRfbr9f_z179WLKvj9hGQxsab9txakW_BIsDKGN3eafrO5MzJKaRuHVDTCMh5pGIYS-OH-gu6HYG8F8z5AkC</recordid><startdate>20220303</startdate><enddate>20220303</enddate><creator>Davies, David J</creator><creator>Hadis, Mohammed</creator><creator>Di Pietro, Valentina</creator><creator>Lazzarino, Giuseppe</creator><creator>Forcione, Mario</creator><creator>Harris, Georgia</creator><creator>Stevens, Andrew R</creator><creator>Soon, Wai Cheong</creator><creator>Goldberg Oppenheimer, Pola</creator><creator>Milward, Michael</creator><creator>Belli, Antonio</creator><creator>Palin, William M</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-3222-133X</orcidid><orcidid>https://orcid.org/0000-0002-4045-8011</orcidid><orcidid>https://orcid.org/0000-0001-8826-7057</orcidid></search><sort><creationdate>20220303</creationdate><title>Photobiomodulation reduces hippocampal apoptotic cell death and produces a Raman spectroscopic "signature"</title><author>Davies, David J ; Hadis, Mohammed ; Di Pietro, Valentina ; Lazzarino, Giuseppe ; Forcione, Mario ; Harris, Georgia ; Stevens, Andrew R ; Soon, Wai Cheong ; Goldberg Oppenheimer, Pola ; Milward, Michael ; Belli, Antonio ; Palin, William M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-b39c0dac5c8d090156eb6d83703c0bbe0c94bdf3e6018ffafcd86e965ddce5b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Aging</topic><topic>Animal tissues</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Biochemistry</topic><topic>Bioengineering</topic><topic>Biology and Life Sciences</topic><topic>Brain</topic><topic>Brain Injuries, Traumatic - 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| subjects | Aging Animal tissues Animals Apoptosis Biochemistry Bioengineering Biology and Life Sciences Brain Brain Injuries, Traumatic - metabolism Brain research Cell death Contusions Dentistry Fibroblasts Head injuries Health aspects Hippocampus Hippocampus - metabolism Hospitals Inflammation Injuries Injury prevention Irradiation Light Light therapy Low-Level Light Therapy - methods Medical research Medicine and Health Sciences Methods Neurosciences Ophthalmology Photobiology Physical Sciences Radiation Raman spectroscopy Rats Research and Analysis Methods Spectroscopic observation Spectrum Analysis, Raman Traumatic brain injury Wound healing |
| title | Photobiomodulation reduces hippocampal apoptotic cell death and produces a Raman spectroscopic "signature" |
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