Comparable respiratory activity in attached and suspended human fibroblasts

Measurement of oxygen consumption of cultured cells is widely used for diagnosis of mitochondrial diseases, drug testing, biotechnology, and toxicology. Fibroblasts are cultured in monolayers, but physiological measurements are carried out in suspended or attached cells. We address the question whet...

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Veröffentlicht in:	PloS one 2022-03, Vol.17 (3), p.e0264496-e0264496
Hauptverfasser:	Zdrazilova, Lucie, Hansikova, Hana, Gnaiger, Erich
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibiotics ATP synthase Biology and Life Sciences Biotechnology Calibration Cell culture Cell Respiration - physiology Electron transfer Fibroblasts Glucose Health aspects Hospitals Humans Medical research Medicine and Health Sciences Medicine, Experimental Metabolic disorders Mitochondria Morphology Oligomycin Oxidative Phosphorylation Oxygen Oxygen consumption Oxygen Consumption - physiology Pediatrics Physical Sciences Physiological aspects Physiology Platforms Reproducibility Reproducibility of Results Residual oxygen Respiration Respirometry Sensors Titration Toxicology
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