Lactoperoxidase potential in diagnosing subclinical mastitis in cows via image processing

This report describes how image processing harnessed to multivariate analysis techniques can be used as a bio-analytical tool for mastitis screening in cows using milk samples collected from 48 animals (32 from Jersey, 7 from Gir, and 9 from Guzerat cow breeds), totalizing a dataset of 144 sequentia...

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Veröffentlicht in:PloS one 2022-02, Vol.17 (2), p.e0263714-e0263714
Hauptverfasser: Silva, Emmanuelle P E, Moraes, Edgar P, Anaya, Katya, Silva, Yhelda M O, Lopes, Heloysa A P, Andrade Neto, Júlio C, Oliveira, Juliana P F, Oliveira, Josenalde B, Rangel, Adriano H N
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container_title PloS one
container_volume 17
creator Silva, Emmanuelle P E
Moraes, Edgar P
Anaya, Katya
Silva, Yhelda M O
Lopes, Heloysa A P
Andrade Neto, Júlio C
Oliveira, Juliana P F
Oliveira, Josenalde B
Rangel, Adriano H N
description This report describes how image processing harnessed to multivariate analysis techniques can be used as a bio-analytical tool for mastitis screening in cows using milk samples collected from 48 animals (32 from Jersey, 7 from Gir, and 9 from Guzerat cow breeds), totalizing a dataset of 144 sequential images was collected and analyzed. In this context, this methodology was developed based on the lactoperoxidase activity to assess mastitis using recorded images of a cuvette during a simple experiment and subsequent image treatments with an R statistics platform. The color of the sample changed from white to brown upon its exposure to reagents, which is a consequence of lactoperoxidase enzymatic reaction. Data analysis was performed to extract the channels from the RGB (Red-Green-Blue) color system, where the resulting dataset was evaluated with Principal Component Analysis (PCA), Multiple Linear Regression (MLR), and Second-Order Regression (SO). Interesting results in terms of enzymatic activity correlation (R2 = 0.96 and R2 = 0.98 by MLR and SO, respectively) and of somatic cell count (R2 = 0.97 and R2 = 0.99 by MLR and SO, respectively), important mastitis indicators, were obtained using this simple method. Additionally, potential advantages can be accessed such as quality control of the dairy chain, easier bovine mastitis prognosis, lower cost, analytical frequency, and could serve as an evaluative parameter to verify the health of the mammary gland.
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potential in diagnosing subclinical mastitis in cows via image processing</title><author>Silva, Emmanuelle P E ; Moraes, Edgar P ; Anaya, Katya ; Silva, Yhelda M O ; Lopes, Heloysa A P ; Andrade Neto, Júlio C ; Oliveira, Juliana P F ; Oliveira, Josenalde B ; Rangel, Adriano H N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-6130a907fc80c417e8124a5144cf4fa1860099dcb4f32aea6abd87dce2a0d5b83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animals</topic><topic>Artificial intelligence</topic><topic>Biology and Life Sciences</topic><topic>Cattle</topic><topic>Color</topic><topic>Computer and Information Sciences</topic><topic>Cost analysis</topic><topic>Cow's milk</topic><topic>Dairy cattle</topic><topic>Data analysis</topic><topic>Datasets</topic><topic>Diagnosis</topic><topic>Engineering and Technology</topic><topic>Enzymatic 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subjects Animals
Artificial intelligence
Biology and Life Sciences
Cattle
Color
Computer and Information Sciences
Cost analysis
Cow's milk
Dairy cattle
Data analysis
Datasets
Diagnosis
Engineering and Technology
Enzymatic activity
Enzymes
Evaluation
Female
Frequency analysis
Health aspects
Image processing
Image Processing, Computer-Assisted - methods
Laboratories
Lactoperoxidase - analysis
Lactoperoxidase - metabolism
Mammary gland
Mammary glands
Mastitis
Mastitis, Bovine - diagnosis
Mastitis, Bovine - enzymology
Medical diagnosis
Medicine and Health Sciences
Methods
Milk
Milk - chemistry
Multivariate analysis
Pathogens
Physical Sciences
Principal components analysis
Quality control
Reagents
Research and Analysis Methods
Smartphones
Statistical analysis
Statistical methods
Universal Serial Bus
title Lactoperoxidase potential in diagnosing subclinical mastitis in cows via image processing
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