Comparative analysis of DNA extraction and PCR product purification methods for cervicovaginal microbiome analysis using cpn60 microbial profiling

The microbiota of the lower female genital tract plays an important role in women's health. Microbial profiling using the chaperonin60 (cpn60) universal target (UT) improves resolution of vaginal species associated with negative health outcomes compared to the more commonly used 16S ribosomal D...

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Veröffentlicht in:	PloS one 2022-01, Vol.17 (1), p.e0262355-e0262355
Hauptverfasser:	Shvartsman, Elinor, Richmond, Meika E I, Schellenberg, John J, Lamont, Alana, Perciani, Catia, Russell, Justen N H, Poliquin, Vanessa, Burgener, Adam, Jaoko, Walter, Sandstrom, Paul, MacDonald, Kelly S
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Bacteria - genetics Biology and Life Sciences Blood Comparative analysis Deoxyribonucleic acid DNA DNA - isolation & purification DNA sequencing DNA, Bacterial - genetics Ecology and Environmental Sciences Ethics Female Genetic aspects Genital tract Gram-positive bacteria Gynecology Health care Humans Immunology Infectious diseases Internal medicine Lysis Lysozyme Medical schools Medicine Medicine and Health Sciences Methods Microbiomes Microbiota Microbiota (Symbiotic organisms) Microbiota - genetics Microorganisms Nucleic Acid Amplification Techniques Obstetrics Polymerase chain reaction Polymerase Chain Reaction - methods Public health Purification Representations Reproducibility Research and analysis methods Ribosomal DNA RNA, Ribosomal, 16S - genetics Sample preparation Secretions Sequence Analysis, DNA - methods Species Species diversity Specimen Handling - methods Vagina Vagina - microbiology Womens health Yield
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