Age-related changes in Kv4/Shal and Kv1/Shaker expression in Drosophila and a role for reactive oxygen species
Age-related changes in ion channel expression are likely to affect neuronal signaling. Here, we examine how age affects Kv4/Shal and Kv1/Shaker K+ channel protein levels in Drosophila. We show that Kv4/Shal protein levels decline sharply from 3 days to 10 days, then more gradually from 10 to 40 days...
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| description | Age-related changes in ion channel expression are likely to affect neuronal signaling. Here, we examine how age affects Kv4/Shal and Kv1/Shaker K+ channel protein levels in Drosophila. We show that Kv4/Shal protein levels decline sharply from 3 days to 10 days, then more gradually from 10 to 40 days after eclosion. In contrast, Kv1/Shaker protein exhibits a transient increase at 10 days that then stabilizes and eventually declines at 40 days. We present data that begin to show a relationship between reactive oxygen species (ROS), Kv4/Shal, and locomotor performance. We show that Kv4/Shal levels are negatively affected by ROS, and that over-expression of Catalase or RNAi knock-down of the ROS-generating enzyme, Nicotinamide Adenine Dinucleotide Phosphate (NADPH) Oxidase (NOX), can attenuate the loss of Kv4/Shal protein. Finally, we compare levels of Kv4.2 and Kv4.3 in the hippocampus, olfactory bulb, cerebellum, and motor cortex of mice aged 6 weeks and 1 year. While there was no global decline in Kv4.2/4.3 that parallels what we report in Drosophila, we did find that Kv4.2/4.3 are differentially affected in various brain regions; this survey of changes may help inform mammalian studies that examine neuronal function with age. |
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Here, we examine how age affects Kv4/Shal and Kv1/Shaker K+ channel protein levels in Drosophila. We show that Kv4/Shal protein levels decline sharply from 3 days to 10 days, then more gradually from 10 to 40 days after eclosion. In contrast, Kv1/Shaker protein exhibits a transient increase at 10 days that then stabilizes and eventually declines at 40 days. We present data that begin to show a relationship between reactive oxygen species (ROS), Kv4/Shal, and locomotor performance. We show that Kv4/Shal levels are negatively affected by ROS, and that over-expression of Catalase or RNAi knock-down of the ROS-generating enzyme, Nicotinamide Adenine Dinucleotide Phosphate (NADPH) Oxidase (NOX), can attenuate the loss of Kv4/Shal protein. Finally, we compare levels of Kv4.2 and Kv4.3 in the hippocampus, olfactory bulb, cerebellum, and motor cortex of mice aged 6 weeks and 1 year. While there was no global decline in Kv4.2/4.3 that parallels what we report in Drosophila, we did find that Kv4.2/4.3 are differentially affected in various brain regions; this survey of changes may help inform mammalian studies that examine neuronal function with age.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0261087</identifier><identifier>PMID: 34932577</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Action Potentials ; Adenine ; Age ; Age Factors ; Aging ; Animals ; Biology and Life Sciences ; Brain ; Catalase ; Cerebellum ; Cortex (motor) ; Cortex (olfactory) ; Drosophila ; Drosophila melanogaster - genetics ; Drosophila melanogaster - metabolism ; Drosophila Proteins - genetics ; Drosophila Proteins - metabolism ; Eclosion ; Fruit flies ; Gene expression ; Insects ; Ion channels ; Laboratories ; Locomotor activity ; Male ; Medicine and Health Sciences ; Memory ; NAD(P)H oxidase ; NADPH-diaphorase ; Neurons - cytology ; Neurons - physiology ; Nicotinamide ; Nicotinamide adenine dinucleotide ; Olfactory bulb ; Overexpression ; Oxygen ; Physical Sciences ; Potassium channels (voltage-gated) ; Proteins ; Quantitative analysis ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Research and Analysis Methods ; RNA-mediated interference ; Shaker Superfamily of Potassium Channels - genetics ; Shaker Superfamily of Potassium Channels - metabolism ; Shal Potassium Channels - genetics ; Shal Potassium Channels - metabolism ; Thermal cycling</subject><ispartof>PloS one, 2021-12, Vol.16 (12), p.e0261087-e0261087</ispartof><rights>2021 Vallejos et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Here, we examine how age affects Kv4/Shal and Kv1/Shaker K+ channel protein levels in Drosophila. We show that Kv4/Shal protein levels decline sharply from 3 days to 10 days, then more gradually from 10 to 40 days after eclosion. In contrast, Kv1/Shaker protein exhibits a transient increase at 10 days that then stabilizes and eventually declines at 40 days. We present data that begin to show a relationship between reactive oxygen species (ROS), Kv4/Shal, and locomotor performance. We show that Kv4/Shal levels are negatively affected by ROS, and that over-expression of Catalase or RNAi knock-down of the ROS-generating enzyme, Nicotinamide Adenine Dinucleotide Phosphate (NADPH) Oxidase (NOX), can attenuate the loss of Kv4/Shal protein. Finally, we compare levels of Kv4.2 and Kv4.3 in the hippocampus, olfactory bulb, cerebellum, and motor cortex of mice aged 6 weeks and 1 year. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vallejos, Maximiliano J</au><au>Eadaim, Abdunaser</au><au>Hahm, Eu-Teum</au><au>Tsunoda, Susan</au><au>Biagini, Giuseppe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Age-related changes in Kv4/Shal and Kv1/Shaker expression in Drosophila and a role for reactive oxygen species</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2021-12-01</date><risdate>2021</risdate><volume>16</volume><issue>12</issue><spage>e0261087</spage><epage>e0261087</epage><pages>e0261087-e0261087</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Age-related changes in ion channel expression are likely to affect neuronal signaling. Here, we examine how age affects Kv4/Shal and Kv1/Shaker K+ channel protein levels in Drosophila. We show that Kv4/Shal protein levels decline sharply from 3 days to 10 days, then more gradually from 10 to 40 days after eclosion. In contrast, Kv1/Shaker protein exhibits a transient increase at 10 days that then stabilizes and eventually declines at 40 days. We present data that begin to show a relationship between reactive oxygen species (ROS), Kv4/Shal, and locomotor performance. We show that Kv4/Shal levels are negatively affected by ROS, and that over-expression of Catalase or RNAi knock-down of the ROS-generating enzyme, Nicotinamide Adenine Dinucleotide Phosphate (NADPH) Oxidase (NOX), can attenuate the loss of Kv4/Shal protein. Finally, we compare levels of Kv4.2 and Kv4.3 in the hippocampus, olfactory bulb, cerebellum, and motor cortex of mice aged 6 weeks and 1 year. While there was no global decline in Kv4.2/4.3 that parallels what we report in Drosophila, we did find that Kv4.2/4.3 are differentially affected in various brain regions; this survey of changes may help inform mammalian studies that examine neuronal function with age.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>34932577</pmid><doi>10.1371/journal.pone.0261087</doi><orcidid>https://orcid.org/0000-0002-5742-8644</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Action Potentials Adenine Age Age Factors Aging Animals Biology and Life Sciences Brain Catalase Cerebellum Cortex (motor) Cortex (olfactory) Drosophila Drosophila melanogaster - genetics Drosophila melanogaster - metabolism Drosophila Proteins - genetics Drosophila Proteins - metabolism Eclosion Fruit flies Gene expression Insects Ion channels Laboratories Locomotor activity Male Medicine and Health Sciences Memory NAD(P)H oxidase NADPH-diaphorase Neurons - cytology Neurons - physiology Nicotinamide Nicotinamide adenine dinucleotide Olfactory bulb Overexpression Oxygen Physical Sciences Potassium channels (voltage-gated) Proteins Quantitative analysis Reactive oxygen species Reactive Oxygen Species - metabolism Research and Analysis Methods RNA-mediated interference Shaker Superfamily of Potassium Channels - genetics Shaker Superfamily of Potassium Channels - metabolism Shal Potassium Channels - genetics Shal Potassium Channels - metabolism Thermal cycling |
| title | Age-related changes in Kv4/Shal and Kv1/Shaker expression in Drosophila and a role for reactive oxygen species |
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