A large-scale transgenic RNAi screen identifies transcription factors that modulate myofiber size in Drosophila
Myofiber atrophy occurs with aging and in many diseases but the underlying mechanisms are incompletely understood. Here, we have used >1,100 muscle-targeted RNAi interventions to comprehensively assess the function of 447 transcription factors in the developmental growth of body wall skeletal mus...
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| creator | Graca, Flavia A Sheffield, Natalie Puppa, Melissa Finkelstein, David Hunt, Liam C Demontis, Fabio |
| description | Myofiber atrophy occurs with aging and in many diseases but the underlying mechanisms are incompletely understood. Here, we have used >1,100 muscle-targeted RNAi interventions to comprehensively assess the function of 447 transcription factors in the developmental growth of body wall skeletal muscles in Drosophila. This screen identifies new regulators of myofiber atrophy and hypertrophy, including the transcription factor Deaf1. Deaf1 RNAi increases myofiber size whereas Deaf1 overexpression induces atrophy. Consistent with its annotation as a Gsk3 phosphorylation substrate, Deaf1 and Gsk3 induce largely overlapping transcriptional changes that are opposed by Deaf1 RNAi. The top category of Deaf1-regulated genes consists of glycolytic enzymes, which are suppressed by Deaf1 and Gsk3 but are upregulated by Deaf1 RNAi. Similar to Deaf1 and Gsk3 overexpression, RNAi for glycolytic enzymes reduces myofiber growth. Altogether, this study defines the repertoire of transcription factors that regulate developmental myofiber growth and the role of Gsk3/Deaf1/glycolysis in this process. |
| doi_str_mv | 10.1371/journal.pgen.1009926 |
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Here, we have used >1,100 muscle-targeted RNAi interventions to comprehensively assess the function of 447 transcription factors in the developmental growth of body wall skeletal muscles in Drosophila. This screen identifies new regulators of myofiber atrophy and hypertrophy, including the transcription factor Deaf1. Deaf1 RNAi increases myofiber size whereas Deaf1 overexpression induces atrophy. Consistent with its annotation as a Gsk3 phosphorylation substrate, Deaf1 and Gsk3 induce largely overlapping transcriptional changes that are opposed by Deaf1 RNAi. The top category of Deaf1-regulated genes consists of glycolytic enzymes, which are suppressed by Deaf1 and Gsk3 but are upregulated by Deaf1 RNAi. Similar to Deaf1 and Gsk3 overexpression, RNAi for glycolytic enzymes reduces myofiber growth. Altogether, this study defines the repertoire of transcription factors that regulate developmental myofiber growth and the role of Gsk3/Deaf1/glycolysis in this process.</description><identifier>ISSN: 1553-7404</identifier><identifier>ISSN: 1553-7390</identifier><identifier>EISSN: 1553-7404</identifier><identifier>DOI: 10.1371/journal.pgen.1009926</identifier><identifier>PMID: 34780463</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adults ; Aging ; Animals ; Animals, Genetically Modified - genetics ; Atrophy ; Biology and life sciences ; Body wall ; Chemical properties ; Disease Models, Animal ; DNA-Binding Proteins - genetics ; Drosophila ; Drosophila melanogaster - genetics ; Drosophila Proteins - genetics ; Embryonic Development - genetics ; Enzymes ; Gene expression ; Genetic aspects ; Genomes ; Glycogen Synthase Kinase 3 - genetics ; Glycolysis ; Glycolysis - genetics ; Humans ; Hypertrophy ; Identification and classification ; Insects ; Kinases ; Medicine and Health Sciences ; Muscle, Skeletal - growth & development ; Muscle, Skeletal - metabolism ; Muscular Atrophy - genetics ; Muscular Atrophy - pathology ; Musculoskeletal system ; Myofibrils - genetics ; Myofibrils - metabolism ; Phosphorylation ; Physiological aspects ; Protein synthesis ; Proteins ; Research and Analysis Methods ; RNA Interference ; RNA-mediated interference ; Skeletal muscle ; Transcription factors ; Transcription Factors - genetics</subject><ispartof>PLoS genetics, 2021-11, Vol.17 (11), p.e1009926-e1009926</ispartof><rights>COPYRIGHT 2021 Public Library of Science</rights><rights>2021 Graca et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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| subjects | Adults Aging Animals Animals, Genetically Modified - genetics Atrophy Biology and life sciences Body wall Chemical properties Disease Models, Animal DNA-Binding Proteins - genetics Drosophila Drosophila melanogaster - genetics Drosophila Proteins - genetics Embryonic Development - genetics Enzymes Gene expression Genetic aspects Genomes Glycogen Synthase Kinase 3 - genetics Glycolysis Glycolysis - genetics Humans Hypertrophy Identification and classification Insects Kinases Medicine and Health Sciences Muscle, Skeletal - growth & development Muscle, Skeletal - metabolism Muscular Atrophy - genetics Muscular Atrophy - pathology Musculoskeletal system Myofibrils - genetics Myofibrils - metabolism Phosphorylation Physiological aspects Protein synthesis Proteins Research and Analysis Methods RNA Interference RNA-mediated interference Skeletal muscle Transcription factors Transcription Factors - genetics |
| title | A large-scale transgenic RNAi screen identifies transcription factors that modulate myofiber size in Drosophila |
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