Validation of a novel molecular assay to the diagnostic of COVID-19 based on real time PCR with high resolution melting

The emergence of the COVID-19 pandemic resulted in an unprecedented need for RT-qPCR-based molecular diagnostic testing, placing a strain on the supply chain and the availability of commercially available PCR testing kits and reagents. The effect of limited molecular diagnostics-related supplies has...

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Veröffentlicht in:	PloS one 2021-11, Vol.16 (11), p.e0260087-e0260087
Hauptverfasser:	Ferreira, Beatriz Iandra da Silva, da Silva-Gomes, Natália Lins, Coelho, Wagner Luis da Costa Nunes Pimentel, da Costa, Vanessa Duarte, Carneiro, Vanessa Cristine de Souza, Kader, Rafael Lopes, Amaro, Marisa Pimentel, Villar, Lívia Melo, Miyajima, Fábio, Alves-Leon, Soniza Vieira, de Paula, Vanessa Salete, Leon, Luciane Almeida Amado, Moreira, Otacilio Cruz
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Assaying Availability Biochemical assays Biology and life sciences Coronaviruses COVID-19 COVID-19 Nucleic Acid Testing - methods COVID-19 Nucleic Acid Testing - standards Developing countries Diagnosis Diagnostic systems Disease transmission Female Genomes Genotyping Hepatitis High resolution Hospitals Humans LDCs Male Medical diagnosis Medical laboratories Medicine and health sciences Melt temperature Melting Methods Molecular biology Molecular diagnostic techniques Nucleic Acid Denaturation Oligonucleotides - chemistry Pandemics Pathogens Patients Physical Sciences Polymerase chain reaction Reagents Real time Real-Time Polymerase Chain Reaction - methods Real-Time Polymerase Chain Reaction - standards Research and Analysis Methods Respiratory Mucosa - virology Ribonuclease P RNA polymerase Saliva Saliva - virology SARS-CoV-2 - genetics SARS-CoV-2 - pathogenicity Sensitivity analysis Sensitivity and Specificity Severe acute respiratory syndrome Severe acute respiratory syndrome coronavirus 2 Supply chains Temperature gradients Viral diseases Virology
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