Heterologous expression and purification of recombinant human protoporphyrinogen oxidase IX: A comparative study

Human protoporphyrinogen oxidase IX (hPPO) is an oxygen-dependent enzyme catalyzing the penultimate step in the heme biosynthesis pathway. Mutations in the enzyme are linked to variegate porphyria, an autosomal dominant metabolic disease. Here we investigated eukaryotic cells as alternative systems...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	PloS one 2021-11, Vol.16 (11), p.e0259837-e0259837
Hauptverfasser:	Novakova, Zora, Khuntsaria, Daria, Gresova, Marketa, Mikesova, Jana, Havlinova, Barbora, Shukla, Shivam, Kolarova, Lucie, Vesela, Katerina, Martasek, Pavel, Barinka, Cyril
Format:	Artikel
Sprache:	eng
Schlagworte:	Agricultural production Analysis Animals Bacteria Biology Biology and Life Sciences Biosynthesis Biotechnology Cell Line Chromatography Cloning Comparative studies E coli Enzymes Escherichia coli - genetics Ethylenediaminetetraacetic acid Flavin-adenine dinucleotide Flavoproteins - biosynthesis Flavoproteins - genetics Flavoproteins - isolation & purification Gene expression Glycerol HEK293 Cells Heme Humans Impact analysis Insects Intracellular Laboratories Mammalian cells Mammals Metabolic disorders Mitochondrial Proteins - biosynthesis Mitochondrial Proteins - genetics Mitochondrial Proteins - isolation & purification Mutation Oxidase Oxygen Physical Sciences Plasmids Porphyria Post-translation Proteins Protoporphyrinogen oxidase Protoporphyrinogen Oxidase - biosynthesis Protoporphyrinogen Oxidase - genetics Protoporphyrinogen Oxidase - isolation & purification Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - isolation & purification Research and Analysis Methods Sf9 Cells
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!