Expression analysis of p16 and TOP2A protein biomarkers in cervical cancer lesions and their correlation with clinico-histopathological characteristics in a referral hospital, Tanzania
Biomarkers yield important information for early diagnosis of cervical cancer. However, they are rarely applied for prognosis of cervical cancer in Tanzania, where visual inspection assay with acetic acid or Lugol's iodine and Pap test are being used as the standard screening/ diagnostic method...
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| description | Biomarkers yield important information for early diagnosis of cervical cancer. However, they are rarely applied for prognosis of cervical cancer in Tanzania, where visual inspection assay with acetic acid or Lugol's iodine and Pap test are being used as the standard screening/ diagnostic methods.
This was a retrospective hospital-based cross-sectional study that was conducted to assess cyclin-dependent kinase inhibitor (p16) and topoisomerase II-alpha (TOP2A) proteins expression among women seeking cervical cancer care at Kilimanjaro Christian Medical Centre, Tanzania between May 1, 2017 and May 10, 2018. Immunohistochemistry technique was used to detect the expressions of p16 and TOP2A proteins from the retrieved formalin-fixed and paraffin-embedded (FFPE) cervical biopsies.
A total of 145 patients, with a mean age of 52.1 ± 12.9 years, were included in this study. Upon immunohistochemistry staining, 103 (71.0%) and 90 (62.1%) were p16 and TOP2A positive respectively. There was a strong association between histopathological class and p16/TOP2A expression levels (Fisher's exact test, p |
| doi_str_mv | 10.1371/journal.pone.0259096 |
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This was a retrospective hospital-based cross-sectional study that was conducted to assess cyclin-dependent kinase inhibitor (p16) and topoisomerase II-alpha (TOP2A) proteins expression among women seeking cervical cancer care at Kilimanjaro Christian Medical Centre, Tanzania between May 1, 2017 and May 10, 2018. Immunohistochemistry technique was used to detect the expressions of p16 and TOP2A proteins from the retrieved formalin-fixed and paraffin-embedded (FFPE) cervical biopsies.
A total of 145 patients, with a mean age of 52.1 ± 12.9 years, were included in this study. Upon immunohistochemistry staining, 103 (71.0%) and 90 (62.1%) were p16 and TOP2A positive respectively. There was a strong association between histopathological class and p16/TOP2A expression levels (Fisher's exact test, p<0.001). Moreover, there was a strong positive correlation between p16/TOP2A and cancerous cervical lesions (Spearman's rank correlation coefficients = 0.833 and 0.687, p = 0.006 and 0.005, respectively). The age-adjusted odds ratio for predicting cervical cancer lesions were independently significant for p16/TOP2A biomarkers in FFPE cervical tissues [p16: OR = 1.142 (95% CI: 1.059-1.232, p<0.001) and TOP2A: OR = 1.046 (95% CI: 1.008-1.085, p = 0.015)]. Importantly, the diagnostic performance of p16 was higher than that of TOP2A in the diagnosis of cancerous lesions from non-cancerous cervical lesions (sensitivity: 97.2% versus 77.6%, accuracy: 92.8% versus 87.8%, respectively).
Our study has highlighted that over-expression of TOP2A is related to the grade of cervical intraepithelial neoplasia but does not predict prognosis in cervical cancer. Similarly, expression of p16 is related to degree of histological dysplasia and malignancy, suggesting its prognostic and predictive value in the management of cervical cancers. Further bigger studies are needed to validate their applications in the early diagnosis of cervical cancer.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0259096</identifier><identifier>PMID: 34705880</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acetic acid ; Analysis ; Biological markers ; Biology and Life Sciences ; Biomarkers ; Biomarkers, Tumor - metabolism ; Biopsy ; Cancer ; Cell cycle ; Cellular biology ; Cervical cancer ; Cervix ; Correlation ; Correlation coefficient ; Correlation coefficients ; Cross-Sectional Studies ; Cyclin-dependent kinase ; Cyclin-Dependent Kinase Inhibitor p16 - metabolism ; Cyclin-dependent kinases ; Diagnosis ; DNA topoisomerase (ATP-hydrolysing) ; DNA Topoisomerases, Type II - metabolism ; Dysplasia ; Enzyme inhibitors ; Feasibility studies ; Female ; Genetic aspects ; Health care facilities ; Hospitals ; Human papillomavirus ; Humans ; Immunohistochemistry ; Inspection ; Iodine ; Kinases ; Lesions ; Malignancy ; Mandela, Nelson (1918-2013) ; Medical diagnosis ; Medical prognosis ; Medical screening ; Medicine and Health Sciences ; Methods ; Overexpression ; Pap smear ; Paraffin ; Paraffins ; People and Places ; Poly-ADP-Ribose Binding Proteins - metabolism ; Prognosis ; Proteins ; Retrospective Studies ; Squamous cell carcinoma ; Tanzania - epidemiology ; Uterine Cervical Neoplasms - diagnosis ; Uterine Cervical Neoplasms - metabolism ; Womens health</subject><ispartof>PloS one, 2021-10, Vol.16 (10), p.e0259096-e0259096</ispartof><rights>COPYRIGHT 2021 Public Library of Science</rights><rights>2021 Zuberi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 Zuberi et al 2021 Zuberi et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-bbd0828810089c2dfd463924bac633efe77fe28edce02afd07c5899a3fda60693</citedby><cites>FETCH-LOGICAL-c692t-bbd0828810089c2dfd463924bac633efe77fe28edce02afd07c5899a3fda60693</cites><orcidid>0000-0003-0150-2005 ; 0000-0001-7226-0168</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8550370/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8550370/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34705880$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zuberi, Zavuga</creatorcontrib><creatorcontrib>Mremi, Alex</creatorcontrib><creatorcontrib>Chilongola, Jaffu O</creatorcontrib><creatorcontrib>Semango, George</creatorcontrib><creatorcontrib>Sauli, Elingarami</creatorcontrib><title>Expression analysis of p16 and TOP2A protein biomarkers in cervical cancer lesions and their correlation with clinico-histopathological characteristics in a referral hospital, Tanzania</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Biomarkers yield important information for early diagnosis of cervical cancer. However, they are rarely applied for prognosis of cervical cancer in Tanzania, where visual inspection assay with acetic acid or Lugol's iodine and Pap test are being used as the standard screening/ diagnostic methods.
This was a retrospective hospital-based cross-sectional study that was conducted to assess cyclin-dependent kinase inhibitor (p16) and topoisomerase II-alpha (TOP2A) proteins expression among women seeking cervical cancer care at Kilimanjaro Christian Medical Centre, Tanzania between May 1, 2017 and May 10, 2018. Immunohistochemistry technique was used to detect the expressions of p16 and TOP2A proteins from the retrieved formalin-fixed and paraffin-embedded (FFPE) cervical biopsies.
A total of 145 patients, with a mean age of 52.1 ± 12.9 years, were included in this study. Upon immunohistochemistry staining, 103 (71.0%) and 90 (62.1%) were p16 and TOP2A positive respectively. There was a strong association between histopathological class and p16/TOP2A expression levels (Fisher's exact test, p<0.001). Moreover, there was a strong positive correlation between p16/TOP2A and cancerous cervical lesions (Spearman's rank correlation coefficients = 0.833 and 0.687, p = 0.006 and 0.005, respectively). The age-adjusted odds ratio for predicting cervical cancer lesions were independently significant for p16/TOP2A biomarkers in FFPE cervical tissues [p16: OR = 1.142 (95% CI: 1.059-1.232, p<0.001) and TOP2A: OR = 1.046 (95% CI: 1.008-1.085, p = 0.015)]. Importantly, the diagnostic performance of p16 was higher than that of TOP2A in the diagnosis of cancerous lesions from non-cancerous cervical lesions (sensitivity: 97.2% versus 77.6%, accuracy: 92.8% versus 87.8%, respectively).
Our study has highlighted that over-expression of TOP2A is related to the grade of cervical intraepithelial neoplasia but does not predict prognosis in cervical cancer. Similarly, expression of p16 is related to degree of histological dysplasia and malignancy, suggesting its prognostic and predictive value in the management of cervical cancers. Further bigger studies are needed to validate their applications in the early diagnosis of cervical cancer.</description><subject>Acetic acid</subject><subject>Analysis</subject><subject>Biological markers</subject><subject>Biology and Life Sciences</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Biopsy</subject><subject>Cancer</subject><subject>Cell cycle</subject><subject>Cellular biology</subject><subject>Cervical cancer</subject><subject>Cervix</subject><subject>Correlation</subject><subject>Correlation coefficient</subject><subject>Correlation coefficients</subject><subject>Cross-Sectional Studies</subject><subject>Cyclin-dependent kinase</subject><subject>Cyclin-Dependent Kinase Inhibitor p16 - metabolism</subject><subject>Cyclin-dependent kinases</subject><subject>Diagnosis</subject><subject>DNA topoisomerase (ATP-hydrolysing)</subject><subject>DNA Topoisomerases, Type II - metabolism</subject><subject>Dysplasia</subject><subject>Enzyme inhibitors</subject><subject>Feasibility studies</subject><subject>Female</subject><subject>Genetic aspects</subject><subject>Health care facilities</subject><subject>Hospitals</subject><subject>Human papillomavirus</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Inspection</subject><subject>Iodine</subject><subject>Kinases</subject><subject>Lesions</subject><subject>Malignancy</subject><subject>Mandela, Nelson (1918-2013)</subject><subject>Medical diagnosis</subject><subject>Medical prognosis</subject><subject>Medical screening</subject><subject>Medicine and Health Sciences</subject><subject>Methods</subject><subject>Overexpression</subject><subject>Pap smear</subject><subject>Paraffin</subject><subject>Paraffins</subject><subject>People and Places</subject><subject>Poly-ADP-Ribose Binding Proteins - metabolism</subject><subject>Prognosis</subject><subject>Proteins</subject><subject>Retrospective Studies</subject><subject>Squamous cell carcinoma</subject><subject>Tanzania - epidemiology</subject><subject>Uterine Cervical Neoplasms - diagnosis</subject><subject>Uterine Cervical Neoplasms - metabolism</subject><subject>Womens health</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9FuFCEUhidGY2v1DYySmBhN3JWBGQZuTDZN1SZNarR6S84yzA6VhSmwtfXJfDyZ3W3TNb0wczHA-f4fzoFTFM9LPC1pU74_96vgwE4H7_QUk1pgwR4U-6WgZMIIpg_vjPeKJzGeY1xTztjjYo9WDa45x_vFn6OrIegYjXcIst11NBH5Dg0ly_MWnZ1-ITM0BJ-0cWhu_BLCTx0iyjOlw6VRYJECl8fI6tEmrnWp1yYg5UPQFtLo_sukHilrnFF-0puY_ACp99YvNh49BFBJhxwxau0PKOhOh5CjvY-DSWDfoTNwv8EZeFo86sBG_Wz7Pyi-fzw6O_w8OTn9dHw4O5koJkiazOct5oTzEmMuFGm7tmJUkGoOilGa7Zum04TrVmlMoGtxo2ouBNCuBYaZoAfFy43vYH2U26JHSWrORCNYVWXieEO0Hs7lEEwu0bX0YOR6wYeFhJBzsloSXQqiO2BCQaXaDgQRjFdN1RHdtIpnrw_b3Vbz5Xgol3L6O6a7EWd6ufCXktc1pg3OBm-2BsFfrHRMcmmi0taC0361PnfT1KTiNKOv_kHvz25LLSAnYFzn875qNJUzxjHlDaMjNb2Hyl-rl_m-ne5MXt8RvN0RZCbpq7SAVYzy-NvX_2dPf-yyr--wvQab-ujtanyCcResNqAKPsb80G6LXGI5NthNNeTYYHLbYFn24u4F3YpuOor-BXQ-JYo</recordid><startdate>20211027</startdate><enddate>20211027</enddate><creator>Zuberi, Zavuga</creator><creator>Mremi, Alex</creator><creator>Chilongola, Jaffu O</creator><creator>Semango, George</creator><creator>Sauli, Elingarami</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-0150-2005</orcidid><orcidid>https://orcid.org/0000-0001-7226-0168</orcidid></search><sort><creationdate>20211027</creationdate><title>Expression analysis of p16 and TOP2A protein biomarkers in cervical cancer lesions and their correlation with clinico-histopathological characteristics in a referral hospital, Tanzania</title><author>Zuberi, Zavuga ; Mremi, Alex ; Chilongola, Jaffu O ; Semango, George ; Sauli, Elingarami</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-bbd0828810089c2dfd463924bac633efe77fe28edce02afd07c5899a3fda60693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Acetic acid</topic><topic>Analysis</topic><topic>Biological markers</topic><topic>Biology and Life Sciences</topic><topic>Biomarkers</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Biopsy</topic><topic>Cancer</topic><topic>Cell cycle</topic><topic>Cellular biology</topic><topic>Cervical cancer</topic><topic>Cervix</topic><topic>Correlation</topic><topic>Correlation coefficient</topic><topic>Correlation coefficients</topic><topic>Cross-Sectional Studies</topic><topic>Cyclin-dependent kinase</topic><topic>Cyclin-Dependent Kinase Inhibitor p16 - metabolism</topic><topic>Cyclin-dependent kinases</topic><topic>Diagnosis</topic><topic>DNA topoisomerase (ATP-hydrolysing)</topic><topic>DNA Topoisomerases, Type II - metabolism</topic><topic>Dysplasia</topic><topic>Enzyme inhibitors</topic><topic>Feasibility studies</topic><topic>Female</topic><topic>Genetic aspects</topic><topic>Health care facilities</topic><topic>Hospitals</topic><topic>Human papillomavirus</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Inspection</topic><topic>Iodine</topic><topic>Kinases</topic><topic>Lesions</topic><topic>Malignancy</topic><topic>Mandela, Nelson (1918-2013)</topic><topic>Medical diagnosis</topic><topic>Medical prognosis</topic><topic>Medical screening</topic><topic>Medicine and Health Sciences</topic><topic>Methods</topic><topic>Overexpression</topic><topic>Pap smear</topic><topic>Paraffin</topic><topic>Paraffins</topic><topic>People and Places</topic><topic>Poly-ADP-Ribose Binding Proteins - metabolism</topic><topic>Prognosis</topic><topic>Proteins</topic><topic>Retrospective Studies</topic><topic>Squamous cell carcinoma</topic><topic>Tanzania - epidemiology</topic><topic>Uterine Cervical Neoplasms - diagnosis</topic><topic>Uterine Cervical Neoplasms - metabolism</topic><topic>Womens health</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zuberi, Zavuga</creatorcontrib><creatorcontrib>Mremi, Alex</creatorcontrib><creatorcontrib>Chilongola, Jaffu O</creatorcontrib><creatorcontrib>Semango, George</creatorcontrib><creatorcontrib>Sauli, Elingarami</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zuberi, Zavuga</au><au>Mremi, Alex</au><au>Chilongola, Jaffu O</au><au>Semango, George</au><au>Sauli, Elingarami</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression analysis of p16 and TOP2A protein biomarkers in cervical cancer lesions and their correlation with clinico-histopathological characteristics in a referral hospital, Tanzania</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2021-10-27</date><risdate>2021</risdate><volume>16</volume><issue>10</issue><spage>e0259096</spage><epage>e0259096</epage><pages>e0259096-e0259096</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Biomarkers yield important information for early diagnosis of cervical cancer. However, they are rarely applied for prognosis of cervical cancer in Tanzania, where visual inspection assay with acetic acid or Lugol's iodine and Pap test are being used as the standard screening/ diagnostic methods.
This was a retrospective hospital-based cross-sectional study that was conducted to assess cyclin-dependent kinase inhibitor (p16) and topoisomerase II-alpha (TOP2A) proteins expression among women seeking cervical cancer care at Kilimanjaro Christian Medical Centre, Tanzania between May 1, 2017 and May 10, 2018. Immunohistochemistry technique was used to detect the expressions of p16 and TOP2A proteins from the retrieved formalin-fixed and paraffin-embedded (FFPE) cervical biopsies.
A total of 145 patients, with a mean age of 52.1 ± 12.9 years, were included in this study. Upon immunohistochemistry staining, 103 (71.0%) and 90 (62.1%) were p16 and TOP2A positive respectively. There was a strong association between histopathological class and p16/TOP2A expression levels (Fisher's exact test, p<0.001). Moreover, there was a strong positive correlation between p16/TOP2A and cancerous cervical lesions (Spearman's rank correlation coefficients = 0.833 and 0.687, p = 0.006 and 0.005, respectively). The age-adjusted odds ratio for predicting cervical cancer lesions were independently significant for p16/TOP2A biomarkers in FFPE cervical tissues [p16: OR = 1.142 (95% CI: 1.059-1.232, p<0.001) and TOP2A: OR = 1.046 (95% CI: 1.008-1.085, p = 0.015)]. Importantly, the diagnostic performance of p16 was higher than that of TOP2A in the diagnosis of cancerous lesions from non-cancerous cervical lesions (sensitivity: 97.2% versus 77.6%, accuracy: 92.8% versus 87.8%, respectively).
Our study has highlighted that over-expression of TOP2A is related to the grade of cervical intraepithelial neoplasia but does not predict prognosis in cervical cancer. Similarly, expression of p16 is related to degree of histological dysplasia and malignancy, suggesting its prognostic and predictive value in the management of cervical cancers. Further bigger studies are needed to validate their applications in the early diagnosis of cervical cancer.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>34705880</pmid><doi>10.1371/journal.pone.0259096</doi><tpages>e0259096</tpages><orcidid>https://orcid.org/0000-0003-0150-2005</orcidid><orcidid>https://orcid.org/0000-0001-7226-0168</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2021-10, Vol.16 (10), p.e0259096-e0259096 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2586979644 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Acetic acid Analysis Biological markers Biology and Life Sciences Biomarkers Biomarkers, Tumor - metabolism Biopsy Cancer Cell cycle Cellular biology Cervical cancer Cervix Correlation Correlation coefficient Correlation coefficients Cross-Sectional Studies Cyclin-dependent kinase Cyclin-Dependent Kinase Inhibitor p16 - metabolism Cyclin-dependent kinases Diagnosis DNA topoisomerase (ATP-hydrolysing) DNA Topoisomerases, Type II - metabolism Dysplasia Enzyme inhibitors Feasibility studies Female Genetic aspects Health care facilities Hospitals Human papillomavirus Humans Immunohistochemistry Inspection Iodine Kinases Lesions Malignancy Mandela, Nelson (1918-2013) Medical diagnosis Medical prognosis Medical screening Medicine and Health Sciences Methods Overexpression Pap smear Paraffin Paraffins People and Places Poly-ADP-Ribose Binding Proteins - metabolism Prognosis Proteins Retrospective Studies Squamous cell carcinoma Tanzania - epidemiology Uterine Cervical Neoplasms - diagnosis Uterine Cervical Neoplasms - metabolism Womens health |
| title | Expression analysis of p16 and TOP2A protein biomarkers in cervical cancer lesions and their correlation with clinico-histopathological characteristics in a referral hospital, Tanzania |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T22%3A59%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20analysis%20of%20p16%20and%20TOP2A%20protein%20biomarkers%20in%20cervical%20cancer%20lesions%20and%20their%20correlation%20with%20clinico-histopathological%20characteristics%20in%20a%20referral%20hospital,%20Tanzania&rft.jtitle=PloS%20one&rft.au=Zuberi,%20Zavuga&rft.date=2021-10-27&rft.volume=16&rft.issue=10&rft.spage=e0259096&rft.epage=e0259096&rft.pages=e0259096-e0259096&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0259096&rft_dat=%3Cgale_plos_%3EA680387634%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2586979644&rft_id=info:pmid/34705880&rft_galeid=A680387634&rft_doaj_id=oai_doaj_org_article_2e192efa69ca4cdfa92968474f2e7dc8&rfr_iscdi=true |