Neuroinductive properties of mGDNF depend on the producer, E. Coli or human cells
The glial cell line-derived neurotrophic factor (GDNF) is involved in the survival of dopaminergic neurons. Besides, GDNF can also induce axonal growth and creation of new functional synapses. GDNF potential is promising for translation to treat diseases associated with neuronal death: neurodegenera...
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| creator | Shamadykova, Dzhirgala V Panteleev, Dmitry Y Kust, Nadezhda N Savchenko, Ekaterina A Rybalkina, Ekaterina Y Revishchin, Alexander V Pavlova, Galina V |
| description | The glial cell line-derived neurotrophic factor (GDNF) is involved in the survival of dopaminergic neurons. Besides, GDNF can also induce axonal growth and creation of new functional synapses. GDNF potential is promising for translation to treat diseases associated with neuronal death: neurodegenerative disorders, ischemic stroke, and cerebral or spinal cord damages. Unproductive clinical trials of GDNF for Parkinson's disease treatment have induced to study this failure. A reason could be due to irrelevant producer cells that cannot perform the required post-translational modifications. The biological activity of recombinant mGDNF produced by E. coli have been compared with mGDNF produced by human cells HEK293. mGDNF variants were tested with PC12 cells, rat embryonic spinal ganglion cells, and SH-SY5Y human neuroblastoma cells in vitro as well as with a mouse model of the Parkinson's disease in vivo. Both in vitro and in vivo the best neuro-inductive ability belongs to mGDNF produced by HEK293 cells. Keywords: GDNF, neural differentiation, bacterial and mammalian expression systems, cell cultures, model of Parkinson's disease. |
| doi_str_mv | 10.1371/journal.pone.0258289 |
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Coli or human cells</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>Public Library of Science (PLoS)</source><creator>Shamadykova, Dzhirgala V ; Panteleev, Dmitry Y ; Kust, Nadezhda N ; Savchenko, Ekaterina A ; Rybalkina, Ekaterina Y ; Revishchin, Alexander V ; Pavlova, Galina V</creator><creatorcontrib>Shamadykova, Dzhirgala V ; Panteleev, Dmitry Y ; Kust, Nadezhda N ; Savchenko, Ekaterina A ; Rybalkina, Ekaterina Y ; Revishchin, Alexander V ; Pavlova, Galina V</creatorcontrib><description>The glial cell line-derived neurotrophic factor (GDNF) is involved in the survival of dopaminergic neurons. Besides, GDNF can also induce axonal growth and creation of new functional synapses. GDNF potential is promising for translation to treat diseases associated with neuronal death: neurodegenerative disorders, ischemic stroke, and cerebral or spinal cord damages. Unproductive clinical trials of GDNF for Parkinson's disease treatment have induced to study this failure. A reason could be due to irrelevant producer cells that cannot perform the required post-translational modifications. The biological activity of recombinant mGDNF produced by E. coli have been compared with mGDNF produced by human cells HEK293. mGDNF variants were tested with PC12 cells, rat embryonic spinal ganglion cells, and SH-SY5Y human neuroblastoma cells in vitro as well as with a mouse model of the Parkinson's disease in vivo. Both in vitro and in vivo the best neuro-inductive ability belongs to mGDNF produced by HEK293 cells. 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This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Coli or human cells</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The glial cell line-derived neurotrophic factor (GDNF) is involved in the survival of dopaminergic neurons. Besides, GDNF can also induce axonal growth and creation of new functional synapses. GDNF potential is promising for translation to treat diseases associated with neuronal death: neurodegenerative disorders, ischemic stroke, and cerebral or spinal cord damages. Unproductive clinical trials of GDNF for Parkinson's disease treatment have induced to study this failure. A reason could be due to irrelevant producer cells that cannot perform the required post-translational modifications. The biological activity of recombinant mGDNF produced by E. coli have been compared with mGDNF produced by human cells HEK293. mGDNF variants were tested with PC12 cells, rat embryonic spinal ganglion cells, and SH-SY5Y human neuroblastoma cells in vitro as well as with a mouse model of the Parkinson's disease in vivo. Both in vitro and in vivo the best neuro-inductive ability belongs to mGDNF produced by HEK293 cells. 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pharmacology</subject><subject>Recombinant Proteins - therapeutic use</subject><subject>Research and Analysis Methods</subject><subject>Spinal cord</subject><subject>Spinal ganglia</subject><subject>Synapses</subject><subject>Tyrosine 3-Monooxygenase - metabolism</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl1rFDEYhQdRbK3-A9GAIAjuOPmYfNwIZW3rQmnx8zZkksxulpnJNJkp-u_NdqdlBxQkFwl5n_ck7-Fk2UtY5BAz-GHrx9CpJu99Z_MClRxx8Sg7hgKjBUUFfnxwPsqexbgtihJzSp9mR5hQTArGjrMvV3YM3nVm1IO7taAPvrdhcDYCX4P24tPVOTC2t50BvgPD5o5IsA3vwVkOlr5xwAewGVvVAW2bJj7PntSqifbFtJ9kP87Pvi8_Ly6vL1bL08uFpgINi7pWhtCKl0QRyLAwStAKc4xUVTKBMUeV0NAoXBDFDDM1hioVSiV0OpYEn2Sv97p946Oc3IgyGVEICHnJE7HaE8arreyDa1X4Lb1y8u7Ch7VUaVTdWMkoq3WFhKi4JgixilGNFISVwrisrE1aH6fXxqq1RttuCKqZic4rndvItb-VvCwIQzAJvJkEgr8ZbRz-8eWJWqv0K9fVPonp1kUtTynjlEHCdqPnf6HSMrZ1OuWhdul-1vBu1pCYwf4a1mqMUa6-ff1_9vrnnH17wG6saoZN9M04ON_FOUj2oA4-xmDrB-dgIXdxvndD7uIspzintleHrj803ecX_wEDD-6x</recordid><startdate>20211011</startdate><enddate>20211011</enddate><creator>Shamadykova, Dzhirgala V</creator><creator>Panteleev, Dmitry Y</creator><creator>Kust, Nadezhda N</creator><creator>Savchenko, Ekaterina A</creator><creator>Rybalkina, Ekaterina Y</creator><creator>Revishchin, Alexander V</creator><creator>Pavlova, Galina V</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-3665-667X</orcidid></search><sort><creationdate>20211011</creationdate><title>Neuroinductive properties of mGDNF depend on the producer, E. 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Coli or human cells</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2021-10-11</date><risdate>2021</risdate><volume>16</volume><issue>10</issue><spage>e0258289</spage><pages>e0258289-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The glial cell line-derived neurotrophic factor (GDNF) is involved in the survival of dopaminergic neurons. Besides, GDNF can also induce axonal growth and creation of new functional synapses. GDNF potential is promising for translation to treat diseases associated with neuronal death: neurodegenerative disorders, ischemic stroke, and cerebral or spinal cord damages. Unproductive clinical trials of GDNF for Parkinson's disease treatment have induced to study this failure. A reason could be due to irrelevant producer cells that cannot perform the required post-translational modifications. The biological activity of recombinant mGDNF produced by E. coli have been compared with mGDNF produced by human cells HEK293. mGDNF variants were tested with PC12 cells, rat embryonic spinal ganglion cells, and SH-SY5Y human neuroblastoma cells in vitro as well as with a mouse model of the Parkinson's disease in vivo. Both in vitro and in vivo the best neuro-inductive ability belongs to mGDNF produced by HEK293 cells. Keywords: GDNF, neural differentiation, bacterial and mammalian expression systems, cell cultures, model of Parkinson's disease.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>34634077</pmid><doi>10.1371/journal.pone.0258289</doi><tpages>e0258289</tpages><orcidid>https://orcid.org/0000-0003-3665-667X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine Animals Atomic properties Biological activity Biology and Life Sciences Cell differentiation Cell survival Clinical trials Diagnosis Disease Models, Animal Dopamine receptors Dorsal root ganglia E coli Engineering and Technology Escherichia coli Experiments Ganglia, Spinal - drug effects Ganglia, Spinal - metabolism Ganglion cells Glial cell line-derived neurotrophic factor Glial Cell Line-Derived Neurotrophic Factor - pharmacology Glial Cell Line-Derived Neurotrophic Factor - therapeutic use Health aspects HEK293 Cells Humans Ischemia Medical research Medical treatment Medicine and Health Sciences Mice Mice, Inbred C57BL Movement disorders Nervous system diseases Neuroblastoma Neuroblastoma cells Neuroblasts Neurodegenerative diseases Neuronal Outgrowth - drug effects Neuronal-glial interactions Neurons - drug effects Neurons - physiology Neurophysiology Neuroprotective Agents - pharmacology Neuroprotective Agents - therapeutic use Parkinson Disease - drug therapy Parkinson Disease - pathology Parkinson's disease PC12 Cells Pheochromocytoma cells Post-translation Producer cells Proteins Rats Recombinant Proteins - pharmacology Recombinant Proteins - therapeutic use Research and Analysis Methods Spinal cord Spinal ganglia Synapses Tyrosine 3-Monooxygenase - metabolism |
| title | Neuroinductive properties of mGDNF depend on the producer, E. Coli or human cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T05%3A30%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Neuroinductive%20properties%20of%20mGDNF%20depend%20on%20the%20producer,%20E.%20Coli%20or%20human%20cells&rft.jtitle=PloS%20one&rft.au=Shamadykova,%20Dzhirgala%20V&rft.date=2021-10-11&rft.volume=16&rft.issue=10&rft.spage=e0258289&rft.pages=e0258289-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0258289&rft_dat=%3Cgale_plos_%3EA678671474%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2580911858&rft_id=info:pmid/34634077&rft_galeid=A678671474&rft_doaj_id=oai_doaj_org_article_767fcb299b8c4227b76c2a11ba335bee&rfr_iscdi=true |