Non-clinical assessment of lubrication and free radical scavenging of an innovative non-animal carboxymethyl chitosan biomaterial for viscosupplementation: An in-vitro and ex-vivo study
Lubrication and free radical scavenging are key features of biomaterials used for viscosupplementation (VS) of joints affected by osteoarthritis (OA). The objective of this study was to describe the non-clinical performance characterization of KiOmedine.sup.® CM-Chitosan, a non-animal carboxymethyl...
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| description | Lubrication and free radical scavenging are key features of biomaterials used for viscosupplementation (VS) of joints affected by osteoarthritis (OA). The objective of this study was to describe the non-clinical performance characterization of KiOmedine.sup.® CM-Chitosan, a non-animal carboxymethyl chitosan, in order to assess its intended action in VS and to compare it to existing viscosupplements based on crosslinked hyaluronan (HA) formulations. The lubrication capacity of the tested viscosupplements (VS) was evaluated in-vitro and ex-vivo. In-vitro, the coefficient of friction (COF) was measured using a novel tribological system. Meanwhile, an ex-vivo biomechanical model in ovine hindlimbs was developed to assess the recovery of join mobility after an intra-articular (IA) injection. Free radical scavenging capacity of HA and KiOmedine.sup.® CM-Chitosan formulations was evaluated using the Trolox Equivalent Antioxidant Capacity (TEAC) assay. In the in-vitro tribological model, KiOmedine.sup.® CM-Chitosan showed high lubrication capacity with a significant COF reduction than crosslinked HA formulations. In the ex-vivo model, the lubrication effect of KiOmedine.sup.® CM-Chitosan following an IA injection in the injured knee was proven again by a COF reduction. The recovery of joint motion was optimal with an IA injection of 3 ml of KiOmedine.sup.® CM-Chitosan, which was significantly better than the crosslinked HA formulation at the same volume. In the in-vitro TEAC assay, KiOmedine.sup.® CM-Chitosan showed a significantly higher free radical scavenging capacity than HA formulations. Overall, the results provide a first insight into the mechanism of action in terms of lubrication and free radical scavenging for the use of KiOmedine.sup.® CM-Chitosan as a VS treatment of OA. KiOmedine.sup.® CM-Chitosan demonstrated a higher capacity to scavenge free radicals, and it showed a higher recovery of mobility after a knee lesion than crosslinked HA formulations. This difference could be explained by the difference in chemical structure between KiOmedine.sup.® CM-Chitosan and HA and their formulations. |
| doi_str_mv | 10.1371/journal.pone.0256770 |
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The objective of this study was to describe the non-clinical performance characterization of KiOmedine.sup.® CM-Chitosan, a non-animal carboxymethyl chitosan, in order to assess its intended action in VS and to compare it to existing viscosupplements based on crosslinked hyaluronan (HA) formulations. The lubrication capacity of the tested viscosupplements (VS) was evaluated in-vitro and ex-vivo. In-vitro, the coefficient of friction (COF) was measured using a novel tribological system. Meanwhile, an ex-vivo biomechanical model in ovine hindlimbs was developed to assess the recovery of join mobility after an intra-articular (IA) injection. Free radical scavenging capacity of HA and KiOmedine.sup.® CM-Chitosan formulations was evaluated using the Trolox Equivalent Antioxidant Capacity (TEAC) assay. In the in-vitro tribological model, KiOmedine.sup.® CM-Chitosan showed high lubrication capacity with a significant COF reduction than crosslinked HA formulations. In the ex-vivo model, the lubrication effect of KiOmedine.sup.® CM-Chitosan following an IA injection in the injured knee was proven again by a COF reduction. The recovery of joint motion was optimal with an IA injection of 3 ml of KiOmedine.sup.® CM-Chitosan, which was significantly better than the crosslinked HA formulation at the same volume. In the in-vitro TEAC assay, KiOmedine.sup.® CM-Chitosan showed a significantly higher free radical scavenging capacity than HA formulations. Overall, the results provide a first insight into the mechanism of action in terms of lubrication and free radical scavenging for the use of KiOmedine.sup.® CM-Chitosan as a VS treatment of OA. KiOmedine.sup.® CM-Chitosan demonstrated a higher capacity to scavenge free radicals, and it showed a higher recovery of mobility after a knee lesion than crosslinked HA formulations. This difference could be explained by the difference in chemical structure between KiOmedine.sup.® CM-Chitosan and HA and their formulations.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0256770</identifier><identifier>PMID: 34634053</identifier><language>eng</language><publisher>San Francisco: Public Library of Science</publisher><subject>Analysis ; Antioxidants ; Arthritis ; Biological products ; Biology and Life Sciences ; Biomaterials ; Biomechanics ; Biomedical materials ; Care and treatment ; Cartilage ; Chitin ; Chitosan ; Coefficient of friction ; Crosslinking ; Engineering and Technology ; Evaluation ; Free radicals ; Friction ; Hyaluronic acid ; Injection ; Injury prevention ; Knee ; Life sciences ; Lubrication ; Mechanical properties ; Medicine and Health Sciences ; Mobility ; Osteoarthritis ; Pain ; Physical Sciences ; Physiology ; R&D ; Recovery ; Research & development ; Rheology ; Scavenging ; Sheep ; Tribology ; Viscoelasticity ; Vitamin E</subject><ispartof>PloS one, 2021-10, Vol.16 (10), p.e0256770-e0256770</ispartof><rights>COPYRIGHT 2021 Public Library of Science</rights><rights>2021 Vandeweerd et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 Vandeweerd et al 2021 Vandeweerd et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c669t-ac2884de5c40c1ecc9b8db77256d4169e0ad4fc4a2562f711ca1d570335ea00d3</citedby><cites>FETCH-LOGICAL-c669t-ac2884de5c40c1ecc9b8db77256d4169e0ad4fc4a2562f711ca1d570335ea00d3</cites><orcidid>0000-0002-3771-2998 ; 0000-0002-2333-8085</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8504732/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8504732/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids></links><search><contributor>Jabbari, Esmaiel</contributor><creatorcontrib>Vandeweerd, Jean-Michel</creatorcontrib><creatorcontrib>Innocenti, Bernado</creatorcontrib><creatorcontrib>Rocasalbas, Guillem</creatorcontrib><creatorcontrib>Gautier, Sandrine Emilia</creatorcontrib><creatorcontrib>Douette, Pierre</creatorcontrib><creatorcontrib>Hermitte, Laurence</creatorcontrib><creatorcontrib>Hontoir, Fanny</creatorcontrib><creatorcontrib>Chausson, Mickael</creatorcontrib><title>Non-clinical assessment of lubrication and free radical scavenging of an innovative non-animal carboxymethyl chitosan biomaterial for viscosupplementation: An in-vitro and ex-vivo study</title><title>PloS one</title><description>Lubrication and free radical scavenging are key features of biomaterials used for viscosupplementation (VS) of joints affected by osteoarthritis (OA). 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This difference could be explained by the difference in chemical structure between KiOmedine.sup.® CM-Chitosan and HA and their formulations.</description><subject>Analysis</subject><subject>Antioxidants</subject><subject>Arthritis</subject><subject>Biological products</subject><subject>Biology and Life Sciences</subject><subject>Biomaterials</subject><subject>Biomechanics</subject><subject>Biomedical materials</subject><subject>Care and treatment</subject><subject>Cartilage</subject><subject>Chitin</subject><subject>Chitosan</subject><subject>Coefficient of friction</subject><subject>Crosslinking</subject><subject>Engineering and Technology</subject><subject>Evaluation</subject><subject>Free radicals</subject><subject>Friction</subject><subject>Hyaluronic acid</subject><subject>Injection</subject><subject>Injury prevention</subject><subject>Knee</subject><subject>Life sciences</subject><subject>Lubrication</subject><subject>Mechanical properties</subject><subject>Medicine and Health 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Emilia</au><au>Douette, Pierre</au><au>Hermitte, Laurence</au><au>Hontoir, Fanny</au><au>Chausson, Mickael</au><au>Jabbari, Esmaiel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Non-clinical assessment of lubrication and free radical scavenging of an innovative non-animal carboxymethyl chitosan biomaterial for viscosupplementation: An in-vitro and ex-vivo study</atitle><jtitle>PloS one</jtitle><date>2021-10-11</date><risdate>2021</risdate><volume>16</volume><issue>10</issue><spage>e0256770</spage><epage>e0256770</epage><pages>e0256770-e0256770</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Lubrication and free radical scavenging are key features of biomaterials used for viscosupplementation (VS) of joints affected by osteoarthritis (OA). The objective of this study was to describe the non-clinical performance characterization of KiOmedine.sup.® CM-Chitosan, a non-animal carboxymethyl chitosan, in order to assess its intended action in VS and to compare it to existing viscosupplements based on crosslinked hyaluronan (HA) formulations. The lubrication capacity of the tested viscosupplements (VS) was evaluated in-vitro and ex-vivo. In-vitro, the coefficient of friction (COF) was measured using a novel tribological system. Meanwhile, an ex-vivo biomechanical model in ovine hindlimbs was developed to assess the recovery of join mobility after an intra-articular (IA) injection. Free radical scavenging capacity of HA and KiOmedine.sup.® CM-Chitosan formulations was evaluated using the Trolox Equivalent Antioxidant Capacity (TEAC) assay. In the in-vitro tribological model, KiOmedine.sup.® CM-Chitosan showed high lubrication capacity with a significant COF reduction than crosslinked HA formulations. In the ex-vivo model, the lubrication effect of KiOmedine.sup.® CM-Chitosan following an IA injection in the injured knee was proven again by a COF reduction. The recovery of joint motion was optimal with an IA injection of 3 ml of KiOmedine.sup.® CM-Chitosan, which was significantly better than the crosslinked HA formulation at the same volume. In the in-vitro TEAC assay, KiOmedine.sup.® CM-Chitosan showed a significantly higher free radical scavenging capacity than HA formulations. Overall, the results provide a first insight into the mechanism of action in terms of lubrication and free radical scavenging for the use of KiOmedine.sup.® CM-Chitosan as a VS treatment of OA. KiOmedine.sup.® CM-Chitosan demonstrated a higher capacity to scavenge free radicals, and it showed a higher recovery of mobility after a knee lesion than crosslinked HA formulations. This difference could be explained by the difference in chemical structure between KiOmedine.sup.® CM-Chitosan and HA and their formulations.</abstract><cop>San Francisco</cop><pub>Public Library of Science</pub><pmid>34634053</pmid><doi>10.1371/journal.pone.0256770</doi><tpages>e0256770</tpages><orcidid>https://orcid.org/0000-0002-3771-2998</orcidid><orcidid>https://orcid.org/0000-0002-2333-8085</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2021-10, Vol.16 (10), p.e0256770-e0256770 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2580911848 |
| source | DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis Antioxidants Arthritis Biological products Biology and Life Sciences Biomaterials Biomechanics Biomedical materials Care and treatment Cartilage Chitin Chitosan Coefficient of friction Crosslinking Engineering and Technology Evaluation Free radicals Friction Hyaluronic acid Injection Injury prevention Knee Life sciences Lubrication Mechanical properties Medicine and Health Sciences Mobility Osteoarthritis Pain Physical Sciences Physiology R&D Recovery Research & development Rheology Scavenging Sheep Tribology Viscoelasticity Vitamin E |
| title | Non-clinical assessment of lubrication and free radical scavenging of an innovative non-animal carboxymethyl chitosan biomaterial for viscosupplementation: An in-vitro and ex-vivo study |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T12%3A36%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Non-clinical%20assessment%20of%20lubrication%20and%20free%20radical%20scavenging%20of%20an%20innovative%20non-animal%20carboxymethyl%20chitosan%20biomaterial%20for%20viscosupplementation:%20An%20in-vitro%20and%20ex-vivo%20study&rft.jtitle=PloS%20one&rft.au=Vandeweerd,%20Jean-Michel&rft.date=2021-10-11&rft.volume=16&rft.issue=10&rft.spage=e0256770&rft.epage=e0256770&rft.pages=e0256770-e0256770&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0256770&rft_dat=%3Cgale_plos_%3EA678671449%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2580911848&rft_id=info:pmid/34634053&rft_galeid=A678671449&rft_doaj_id=oai_doaj_org_article_9cca2761451c44dfbb8a0f5e8bd5a245&rfr_iscdi=true |