Survey and molecular detection of Sri Lankan cassava mosaic virus in Thailand
Cassava plantations in an area of 458 hectares spanning five provinces along the Thailand-Cambodia border were surveyed from October 2018 to July 2019 to determine the prevalence of cassava mosaic disease (CMD) caused by Sri Lankan cassava mosaic virus (SLCMV) in the region. CMD prevalence was 40% i...
Gespeichert in:
| Veröffentlicht in: | PloS one 2021-10, Vol.16 (10), p.e0252846-e0252846 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0252846 |
|---|---|
| container_issue | 10 |
| container_start_page | e0252846 |
| container_title | PloS one |
| container_volume | 16 |
| creator | Saokham, Kingkan Hemniam, Nuannapa Roekwan, Sukanya Hunsawattanakul, Sirikan Thawinampan, Jutathip Siriwan, Wanwisa |
| description | Cassava plantations in an area of 458 hectares spanning five provinces along the Thailand-Cambodia border were surveyed from October 2018 to July 2019 to determine the prevalence of cassava mosaic disease (CMD) caused by Sri Lankan cassava mosaic virus (SLCMV) in the region. CMD prevalence was 40% in the whole area and 80% in Prachinburi, 43% in Sakaeo, 37% in Burium, 25% in Surin, and 19% in Sisaket provinces. Disease incidence of CMD was highest 43.08% in Sakaeo, followed by 26.78% in Prachinburi, 7% in Burium, 2.58% in Surin, and 1.25% in Sisaket provinces. Disease severity of CMD symptoms was mild chlorosis to moderate mosaic (2-3). The greatest disease severity was recorded in Prachinburi and Sakaeo provinces. Asymptomatic plants were identified in Surin (12%), Prachinburi (5%), Sakaeo (0.2%), and Buriram (0.1%) by PCR analysis. Cassava cultivars CMR-89 and Huai Bong 80 were susceptible to CMD. In 95% of cases, the infection was transmitted by whiteflies (Bemisia tabaci), which were abundant in Sakaeo, Buriram, and Prachinburi but were sparse in Surin; their densities were highest in May and June 2019. Nucleotide sequencing of the mitochondrial cytochrome oxidase 1 (mtCO1) gene of whiteflies in Thailand revealed that it was similar to the mtCO1 gene of Asia II 1 whitefly. Furthermore, the AV1 gene of SLCMV-which encodes the capsid protein-showed 90% nucleotide identity with SLCMV. Phylogenetic analysis of completed nucleotide sequences of DNA-A and DNA-B components of the SLCMV genome determined by rolling circle amplification (RCA) indicated that they were similar to the nucleotide sequence of SLCMV isolates from Thailand, Vietnam, and Cambodia. These results provide important insights into the distribution, impact, and spread of CMD and SLCMV in Thailand. |
| doi_str_mv | 10.1371/journal.pone.0252846 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2580911369</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A678671484</galeid><doaj_id>oai_doaj_org_article_9085f53eb2504a39b3aa645db2c10dae</doaj_id><sourcerecordid>A678671484</sourcerecordid><originalsourceid>FETCH-LOGICAL-c669t-2559f02db6d28a411e98fd5d08e1043289bf43864251f7eb4d82aae78fdb8c6b3</originalsourceid><addsrcrecordid>eNqNkl1rFDEUhgdRbK3-A8EBQfRi13xP5kYoxY-FlYJbvQ1nksxu1uykTWYW--_NdkfpSC8kFwknz3nPB29RvMRojmmF32_DEDvw8-vQ2TkinEgmHhWnuKZkJgiij--9T4pnKW0R4lQK8bQ4oUxQhig7Lb6uhri3tyV0ptwFb_XgIZbG9lb3LnRlaMtVdOUSup_QlRpSgj1kMoHT5d7FIZWuK6824HyWeF48acEn-2K8z4rvnz5eXXyZLS8_Ly7OlzMtRN3PCOd1i4hphCESGMa2lq3hBkmLEaNE1k3LcquMcNxWtmFGEgBbZaiRWjT0rHh11L32IalxE0kRLlGNMRV1JhZHwgTYquvodhBvVQCn7gIhrhXE3mlvVY0kbzm1DeGIAa0bCiAYNw3RGBmwWevDWG1odtZo2_UR_ER0-tO5jVqHvZJZsCI8C7wdBWK4GWzq1c4lbX1emQ3DXd-YVDXjB_T1P-jD043UGvIArmtDrqsPoupcVFJUmEmWqfkDVD7G7pzOtmldjk8S3k0SMtPbX_0ahpTUYvXt_9nLH1P2zT12Y8H3mxT8cHBYmoLsCOoYUoq2_btkjNTB9X-2oQ6uV6Pr6W-pNPIb</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2580911369</pqid></control><display><type>article</type><title>Survey and molecular detection of Sri Lankan cassava mosaic virus in Thailand</title><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Saokham, Kingkan ; Hemniam, Nuannapa ; Roekwan, Sukanya ; Hunsawattanakul, Sirikan ; Thawinampan, Jutathip ; Siriwan, Wanwisa</creator><contributor>Pappu, Hanu R</contributor><creatorcontrib>Saokham, Kingkan ; Hemniam, Nuannapa ; Roekwan, Sukanya ; Hunsawattanakul, Sirikan ; Thawinampan, Jutathip ; Siriwan, Wanwisa ; Pappu, Hanu R</creatorcontrib><description>Cassava plantations in an area of 458 hectares spanning five provinces along the Thailand-Cambodia border were surveyed from October 2018 to July 2019 to determine the prevalence of cassava mosaic disease (CMD) caused by Sri Lankan cassava mosaic virus (SLCMV) in the region. CMD prevalence was 40% in the whole area and 80% in Prachinburi, 43% in Sakaeo, 37% in Burium, 25% in Surin, and 19% in Sisaket provinces. Disease incidence of CMD was highest 43.08% in Sakaeo, followed by 26.78% in Prachinburi, 7% in Burium, 2.58% in Surin, and 1.25% in Sisaket provinces. Disease severity of CMD symptoms was mild chlorosis to moderate mosaic (2-3). The greatest disease severity was recorded in Prachinburi and Sakaeo provinces. Asymptomatic plants were identified in Surin (12%), Prachinburi (5%), Sakaeo (0.2%), and Buriram (0.1%) by PCR analysis. Cassava cultivars CMR-89 and Huai Bong 80 were susceptible to CMD. In 95% of cases, the infection was transmitted by whiteflies (Bemisia tabaci), which were abundant in Sakaeo, Buriram, and Prachinburi but were sparse in Surin; their densities were highest in May and June 2019. Nucleotide sequencing of the mitochondrial cytochrome oxidase 1 (mtCO1) gene of whiteflies in Thailand revealed that it was similar to the mtCO1 gene of Asia II 1 whitefly. Furthermore, the AV1 gene of SLCMV-which encodes the capsid protein-showed 90% nucleotide identity with SLCMV. Phylogenetic analysis of completed nucleotide sequences of DNA-A and DNA-B components of the SLCMV genome determined by rolling circle amplification (RCA) indicated that they were similar to the nucleotide sequence of SLCMV isolates from Thailand, Vietnam, and Cambodia. These results provide important insights into the distribution, impact, and spread of CMD and SLCMV in Thailand.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0252846</identifier><identifier>PMID: 34634034</identifier><language>eng</language><publisher>San Francisco: Public Library of Science</publisher><subject>Agricultural biotechnology ; Agriculture ; Aleyrodidae ; Analysis ; Atomic properties ; Biology and Life Sciences ; Capsid protein ; Cassava ; Chlorosis ; Cultivars ; Cytochrome ; Cytochromes ; Deoxyribonucleic acid ; Disease ; Disease transmission ; Diseases and pests ; DNA ; Flowers & plants ; Gene sequencing ; Genomes ; Medicine and Health Sciences ; Mitochondria ; Mosaic disease ; Nucleotide sequence ; Nucleotides ; People and Places ; Phylogeny ; Plant pathology ; Plantations ; Provinces ; Research and Analysis Methods ; Signs and symptoms ; Viruses</subject><ispartof>PloS one, 2021-10, Vol.16 (10), p.e0252846-e0252846</ispartof><rights>COPYRIGHT 2021 Public Library of Science</rights><rights>2021 Saokham et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 Saokham et al 2021 Saokham et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c669t-2559f02db6d28a411e98fd5d08e1043289bf43864251f7eb4d82aae78fdb8c6b3</citedby><cites>FETCH-LOGICAL-c669t-2559f02db6d28a411e98fd5d08e1043289bf43864251f7eb4d82aae78fdb8c6b3</cites><orcidid>0000-0002-5603-7844</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8504725/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8504725/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids></links><search><contributor>Pappu, Hanu R</contributor><creatorcontrib>Saokham, Kingkan</creatorcontrib><creatorcontrib>Hemniam, Nuannapa</creatorcontrib><creatorcontrib>Roekwan, Sukanya</creatorcontrib><creatorcontrib>Hunsawattanakul, Sirikan</creatorcontrib><creatorcontrib>Thawinampan, Jutathip</creatorcontrib><creatorcontrib>Siriwan, Wanwisa</creatorcontrib><title>Survey and molecular detection of Sri Lankan cassava mosaic virus in Thailand</title><title>PloS one</title><description>Cassava plantations in an area of 458 hectares spanning five provinces along the Thailand-Cambodia border were surveyed from October 2018 to July 2019 to determine the prevalence of cassava mosaic disease (CMD) caused by Sri Lankan cassava mosaic virus (SLCMV) in the region. CMD prevalence was 40% in the whole area and 80% in Prachinburi, 43% in Sakaeo, 37% in Burium, 25% in Surin, and 19% in Sisaket provinces. Disease incidence of CMD was highest 43.08% in Sakaeo, followed by 26.78% in Prachinburi, 7% in Burium, 2.58% in Surin, and 1.25% in Sisaket provinces. Disease severity of CMD symptoms was mild chlorosis to moderate mosaic (2-3). The greatest disease severity was recorded in Prachinburi and Sakaeo provinces. Asymptomatic plants were identified in Surin (12%), Prachinburi (5%), Sakaeo (0.2%), and Buriram (0.1%) by PCR analysis. Cassava cultivars CMR-89 and Huai Bong 80 were susceptible to CMD. In 95% of cases, the infection was transmitted by whiteflies (Bemisia tabaci), which were abundant in Sakaeo, Buriram, and Prachinburi but were sparse in Surin; their densities were highest in May and June 2019. Nucleotide sequencing of the mitochondrial cytochrome oxidase 1 (mtCO1) gene of whiteflies in Thailand revealed that it was similar to the mtCO1 gene of Asia II 1 whitefly. Furthermore, the AV1 gene of SLCMV-which encodes the capsid protein-showed 90% nucleotide identity with SLCMV. Phylogenetic analysis of completed nucleotide sequences of DNA-A and DNA-B components of the SLCMV genome determined by rolling circle amplification (RCA) indicated that they were similar to the nucleotide sequence of SLCMV isolates from Thailand, Vietnam, and Cambodia. These results provide important insights into the distribution, impact, and spread of CMD and SLCMV in Thailand.</description><subject>Agricultural biotechnology</subject><subject>Agriculture</subject><subject>Aleyrodidae</subject><subject>Analysis</subject><subject>Atomic properties</subject><subject>Biology and Life Sciences</subject><subject>Capsid protein</subject><subject>Cassava</subject><subject>Chlorosis</subject><subject>Cultivars</subject><subject>Cytochrome</subject><subject>Cytochromes</subject><subject>Deoxyribonucleic acid</subject><subject>Disease</subject><subject>Disease transmission</subject><subject>Diseases and pests</subject><subject>DNA</subject><subject>Flowers & plants</subject><subject>Gene sequencing</subject><subject>Genomes</subject><subject>Medicine and Health Sciences</subject><subject>Mitochondria</subject><subject>Mosaic disease</subject><subject>Nucleotide sequence</subject><subject>Nucleotides</subject><subject>People and Places</subject><subject>Phylogeny</subject><subject>Plant pathology</subject><subject>Plantations</subject><subject>Provinces</subject><subject>Research and Analysis Methods</subject><subject>Signs and symptoms</subject><subject>Viruses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl1rFDEUhgdRbK3-A8EBQfRi13xP5kYoxY-FlYJbvQ1nksxu1uykTWYW--_NdkfpSC8kFwknz3nPB29RvMRojmmF32_DEDvw8-vQ2TkinEgmHhWnuKZkJgiij--9T4pnKW0R4lQK8bQ4oUxQhig7Lb6uhri3tyV0ptwFb_XgIZbG9lb3LnRlaMtVdOUSup_QlRpSgj1kMoHT5d7FIZWuK6824HyWeF48acEn-2K8z4rvnz5eXXyZLS8_Ly7OlzMtRN3PCOd1i4hphCESGMa2lq3hBkmLEaNE1k3LcquMcNxWtmFGEgBbZaiRWjT0rHh11L32IalxE0kRLlGNMRV1JhZHwgTYquvodhBvVQCn7gIhrhXE3mlvVY0kbzm1DeGIAa0bCiAYNw3RGBmwWevDWG1odtZo2_UR_ER0-tO5jVqHvZJZsCI8C7wdBWK4GWzq1c4lbX1emQ3DXd-YVDXjB_T1P-jD043UGvIArmtDrqsPoupcVFJUmEmWqfkDVD7G7pzOtmldjk8S3k0SMtPbX_0ahpTUYvXt_9nLH1P2zT12Y8H3mxT8cHBYmoLsCOoYUoq2_btkjNTB9X-2oQ6uV6Pr6W-pNPIb</recordid><startdate>20211011</startdate><enddate>20211011</enddate><creator>Saokham, Kingkan</creator><creator>Hemniam, Nuannapa</creator><creator>Roekwan, Sukanya</creator><creator>Hunsawattanakul, Sirikan</creator><creator>Thawinampan, Jutathip</creator><creator>Siriwan, Wanwisa</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-5603-7844</orcidid></search><sort><creationdate>20211011</creationdate><title>Survey and molecular detection of Sri Lankan cassava mosaic virus in Thailand</title><author>Saokham, Kingkan ; Hemniam, Nuannapa ; Roekwan, Sukanya ; Hunsawattanakul, Sirikan ; Thawinampan, Jutathip ; Siriwan, Wanwisa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c669t-2559f02db6d28a411e98fd5d08e1043289bf43864251f7eb4d82aae78fdb8c6b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Agricultural biotechnology</topic><topic>Agriculture</topic><topic>Aleyrodidae</topic><topic>Analysis</topic><topic>Atomic properties</topic><topic>Biology and Life Sciences</topic><topic>Capsid protein</topic><topic>Cassava</topic><topic>Chlorosis</topic><topic>Cultivars</topic><topic>Cytochrome</topic><topic>Cytochromes</topic><topic>Deoxyribonucleic acid</topic><topic>Disease</topic><topic>Disease transmission</topic><topic>Diseases and pests</topic><topic>DNA</topic><topic>Flowers & plants</topic><topic>Gene sequencing</topic><topic>Genomes</topic><topic>Medicine and Health Sciences</topic><topic>Mitochondria</topic><topic>Mosaic disease</topic><topic>Nucleotide sequence</topic><topic>Nucleotides</topic><topic>People and Places</topic><topic>Phylogeny</topic><topic>Plant pathology</topic><topic>Plantations</topic><topic>Provinces</topic><topic>Research and Analysis Methods</topic><topic>Signs and symptoms</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saokham, Kingkan</creatorcontrib><creatorcontrib>Hemniam, Nuannapa</creatorcontrib><creatorcontrib>Roekwan, Sukanya</creatorcontrib><creatorcontrib>Hunsawattanakul, Sirikan</creatorcontrib><creatorcontrib>Thawinampan, Jutathip</creatorcontrib><creatorcontrib>Siriwan, Wanwisa</creatorcontrib><collection>CrossRef</collection><collection>Gale in Context : Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing and Allied Health Journals</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saokham, Kingkan</au><au>Hemniam, Nuannapa</au><au>Roekwan, Sukanya</au><au>Hunsawattanakul, Sirikan</au><au>Thawinampan, Jutathip</au><au>Siriwan, Wanwisa</au><au>Pappu, Hanu R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Survey and molecular detection of Sri Lankan cassava mosaic virus in Thailand</atitle><jtitle>PloS one</jtitle><date>2021-10-11</date><risdate>2021</risdate><volume>16</volume><issue>10</issue><spage>e0252846</spage><epage>e0252846</epage><pages>e0252846-e0252846</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Cassava plantations in an area of 458 hectares spanning five provinces along the Thailand-Cambodia border were surveyed from October 2018 to July 2019 to determine the prevalence of cassava mosaic disease (CMD) caused by Sri Lankan cassava mosaic virus (SLCMV) in the region. CMD prevalence was 40% in the whole area and 80% in Prachinburi, 43% in Sakaeo, 37% in Burium, 25% in Surin, and 19% in Sisaket provinces. Disease incidence of CMD was highest 43.08% in Sakaeo, followed by 26.78% in Prachinburi, 7% in Burium, 2.58% in Surin, and 1.25% in Sisaket provinces. Disease severity of CMD symptoms was mild chlorosis to moderate mosaic (2-3). The greatest disease severity was recorded in Prachinburi and Sakaeo provinces. Asymptomatic plants were identified in Surin (12%), Prachinburi (5%), Sakaeo (0.2%), and Buriram (0.1%) by PCR analysis. Cassava cultivars CMR-89 and Huai Bong 80 were susceptible to CMD. In 95% of cases, the infection was transmitted by whiteflies (Bemisia tabaci), which were abundant in Sakaeo, Buriram, and Prachinburi but were sparse in Surin; their densities were highest in May and June 2019. Nucleotide sequencing of the mitochondrial cytochrome oxidase 1 (mtCO1) gene of whiteflies in Thailand revealed that it was similar to the mtCO1 gene of Asia II 1 whitefly. Furthermore, the AV1 gene of SLCMV-which encodes the capsid protein-showed 90% nucleotide identity with SLCMV. Phylogenetic analysis of completed nucleotide sequences of DNA-A and DNA-B components of the SLCMV genome determined by rolling circle amplification (RCA) indicated that they were similar to the nucleotide sequence of SLCMV isolates from Thailand, Vietnam, and Cambodia. These results provide important insights into the distribution, impact, and spread of CMD and SLCMV in Thailand.</abstract><cop>San Francisco</cop><pub>Public Library of Science</pub><pmid>34634034</pmid><doi>10.1371/journal.pone.0252846</doi><tpages>e0252846</tpages><orcidid>https://orcid.org/0000-0002-5603-7844</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2021-10, Vol.16 (10), p.e0252846-e0252846 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2580911369 |
| source | DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Agricultural biotechnology Agriculture Aleyrodidae Analysis Atomic properties Biology and Life Sciences Capsid protein Cassava Chlorosis Cultivars Cytochrome Cytochromes Deoxyribonucleic acid Disease Disease transmission Diseases and pests DNA Flowers & plants Gene sequencing Genomes Medicine and Health Sciences Mitochondria Mosaic disease Nucleotide sequence Nucleotides People and Places Phylogeny Plant pathology Plantations Provinces Research and Analysis Methods Signs and symptoms Viruses |
| title | Survey and molecular detection of Sri Lankan cassava mosaic virus in Thailand |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T22%3A15%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Survey%20and%20molecular%20detection%20of%20Sri%20Lankan%20cassava%20mosaic%20virus%20in%20Thailand&rft.jtitle=PloS%20one&rft.au=Saokham,%20Kingkan&rft.date=2021-10-11&rft.volume=16&rft.issue=10&rft.spage=e0252846&rft.epage=e0252846&rft.pages=e0252846-e0252846&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0252846&rft_dat=%3Cgale_plos_%3EA678671484%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2580911369&rft_id=info:pmid/34634034&rft_galeid=A678671484&rft_doaj_id=oai_doaj_org_article_9085f53eb2504a39b3aa645db2c10dae&rfr_iscdi=true |