Comparison of methods for pre-processing, exosome isolation, and RNA extraction in unpasteurized bovine and human milk

Milk is a highly complex, heterogeneous biological fluid that contains non-nutritive, bioactive extracellular vesicles called exosomes. Characterization of milk-derived exosomes (MDEs) is challenging due to the lack of standardized methods that are currently being used for milk pre-processing, stora...

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Veröffentlicht in:	PloS one 2021-09, Vol.16 (9), p.e0257633
Hauptverfasser:	Wijenayake, Sanoji, Eisha, Shafinaz, Tawhidi, Zoya, Pitino, Michael A, Steele, Michael A, Fleming, Alison S, McGowan, Patrick O
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Animals Bioengineering Biology and Life Sciences Breast milk Calnexin Casein Cattle CD63 antigen CD9 antigen Concentrators Cow's milk Cream Electron microscopy Epigenetics Exosomes Exosomes - chemistry Exosomes - metabolism Extracellular vesicles Female Filtration Fractional Precipitation Fractionation Genetic aspects Globules Health aspects Humans Immunoblotting Lipids Medicine and Health Sciences Methods MicroRNAs Microscopy, Electron, Transmission Milk Milk - metabolism Milk, Human - metabolism Nanoparticles Proteins Research and analysis methods Ribonucleic acid RNA RNA - isolation & purification RNA - metabolism RNA processing Sample preparation Transmission electron microscopy Ultracentrifugation Viscosity
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