Evaluation of a natural compound extracted from Dolichandrone atrovirens as a novel antioxidant agent using Caenorhabditis elegans
The compound methyl cinnamoyl catalpol (DAM-1) was isolated from the methanol extract of Dolichandrone atrovirens. Studies have already reported the antioxidant activity of Dolichandrone atrovirens bark extract, but till date the antioxidant activity of the isolated compound DAM-1, remains unexplore...
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| Veröffentlicht in: | PloS one 2021-09, Vol.16 (9), p.e0257702 |
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| description | The compound methyl cinnamoyl catalpol (DAM-1) was isolated from the methanol extract of Dolichandrone atrovirens. Studies have already reported the antioxidant activity of Dolichandrone atrovirens bark extract, but till date the antioxidant activity of the isolated compound DAM-1, remains unexplored. The endogenous process of reactive oxygen species generation which leads to various degenerative diseases, can be broken down using these exogenous moieties from plant origin, herein this study we sought to evaluate the antioxidant potential of the DAM-1 compound using Caenorhabditis elegans (C. elegans), which is the primary model to study the antioxidant activity of compounds. Cytotoxicity assay results showed that DAM-1 treatment in the concentration of 10, 25 and 50 μg/ml has shown 100%, 91%, and 50% survival respectively with overall p |
| doi_str_mv | 10.1371/journal.pone.0257702 |
| format | Article |
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Studies have already reported the antioxidant activity of Dolichandrone atrovirens bark extract, but till date the antioxidant activity of the isolated compound DAM-1, remains unexplored. The endogenous process of reactive oxygen species generation which leads to various degenerative diseases, can be broken down using these exogenous moieties from plant origin, herein this study we sought to evaluate the antioxidant potential of the DAM-1 compound using Caenorhabditis elegans (C. elegans), which is the primary model to study the antioxidant activity of compounds. Cytotoxicity assay results showed that DAM-1 treatment in the concentration of 10, 25 and 50 μg/ml has shown 100%, 91%, and 50% survival respectively with overall p<0.0001 (treatment v/s control group). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide-Formazan (MTT) assay results showed that treatment had better survival rates than the control group at different time intervals i.e. 48 h, and 72 h with p<0.01. Mechanosensation (behavioral study) as well as in vivo study results showed that at 0 h, 10 μg/ml of DAM-1 treatment showed a better anti-oxidative activity than the control group, 25 and 50 μg/ml of DAM-1 treated groups with p<0.001 but at 2.5 h incubation with 10, 25, 50 μg/ml of DAM-1 showed an increased anti-oxidative activity than the control group with p<0.001. Thermoresistance assay confirmed that the treatment group had more survival than control group with p<0.001. Absorption study of DAM-1 in C. elegans has shown that the absorption of the drug increases up to 180 mins with a slight decrease after 360 mins and then constant absorption up to 1440 mins. This study paves the way towards the initiative to explore the pharmacological role of DAM-1 in various oxidative stress mediated diseases at molecular levels and the absorption study points out its potential role which could be utilized in the metabolomics and proteomics analysis of this compound in other studies.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0257702</identifier><identifier>PMID: 34551009</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Absorption ; Animals ; Antioxidants ; Antioxidants - chemistry ; Antioxidants - pharmacology ; Assaying ; Bark ; Biocompatibility ; Biological Products - chemistry ; Biological Products - pharmacology ; Biology and Life Sciences ; Bromides ; Caenorhabditis elegans ; Caenorhabditis elegans - drug effects ; Caenorhabditis elegans - metabolism ; Cytotoxicity ; Degenerative diseases ; Diabetes ; Dolichandrone ; Education ; Enzymes ; Food ; In vivo methods and tests ; Iridoid Glucosides - pharmacology ; Lamiales ; Mechanotransduction ; Medicine and Health Sciences ; Metabolites ; Metabolomics ; Natural products ; Oxidative stress ; Oxidative Stress - drug effects ; Oxygen ; Pharmaceutical sciences ; Pharmacology ; Phytochemicals ; Plant extracts ; Plant Extracts - chemistry ; Plant Extracts - pharmacology ; Properties ; Proteomics ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Research and Analysis Methods ; Solvents ; Survival ; Toxicity ; Toxicology</subject><ispartof>PloS one, 2021-09, Vol.16 (9), p.e0257702</ispartof><rights>COPYRIGHT 2021 Public Library of Science</rights><rights>2021 Yellurkar et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 Yellurkar et al 2021 Yellurkar et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-ce8fe0b184235d89d0985c11e34577e70ffae8677064558e97e6b8bfec4c91c03</citedby><cites>FETCH-LOGICAL-c692t-ce8fe0b184235d89d0985c11e34577e70ffae8677064558e97e6b8bfec4c91c03</cites><orcidid>0000-0002-2096-2552 ; 0000-0002-9627-6890</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8457486/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8457486/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53770,53772,79347,79348</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34551009$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Agbor, Gabriel</contributor><creatorcontrib>Yellurkar, Manoj Limbraj</creatorcontrib><creatorcontrib>Singh, Vibhavana</creatorcontrib><creatorcontrib>Sai Prasanna, Vani</creatorcontrib><creatorcontrib>Das, Pamelika</creatorcontrib><creatorcontrib>Nanjappan, Satheeshkumar</creatorcontrib><creatorcontrib>Velayutham, Ravichandiran</creatorcontrib><creatorcontrib>Arumugam, Somasundaram</creatorcontrib><title>Evaluation of a natural compound extracted from Dolichandrone atrovirens as a novel antioxidant agent using Caenorhabditis elegans</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The compound methyl cinnamoyl catalpol (DAM-1) was isolated from the methanol extract of Dolichandrone atrovirens. Studies have already reported the antioxidant activity of Dolichandrone atrovirens bark extract, but till date the antioxidant activity of the isolated compound DAM-1, remains unexplored. The endogenous process of reactive oxygen species generation which leads to various degenerative diseases, can be broken down using these exogenous moieties from plant origin, herein this study we sought to evaluate the antioxidant potential of the DAM-1 compound using Caenorhabditis elegans (C. elegans), which is the primary model to study the antioxidant activity of compounds. Cytotoxicity assay results showed that DAM-1 treatment in the concentration of 10, 25 and 50 μg/ml has shown 100%, 91%, and 50% survival respectively with overall p<0.0001 (treatment v/s control group). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide-Formazan (MTT) assay results showed that treatment had better survival rates than the control group at different time intervals i.e. 48 h, and 72 h with p<0.01. Mechanosensation (behavioral study) as well as in vivo study results showed that at 0 h, 10 μg/ml of DAM-1 treatment showed a better anti-oxidative activity than the control group, 25 and 50 μg/ml of DAM-1 treated groups with p<0.001 but at 2.5 h incubation with 10, 25, 50 μg/ml of DAM-1 showed an increased anti-oxidative activity than the control group with p<0.001. Thermoresistance assay confirmed that the treatment group had more survival than control group with p<0.001. Absorption study of DAM-1 in C. elegans has shown that the absorption of the drug increases up to 180 mins with a slight decrease after 360 mins and then constant absorption up to 1440 mins. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yellurkar, Manoj Limbraj</au><au>Singh, Vibhavana</au><au>Sai Prasanna, Vani</au><au>Das, Pamelika</au><au>Nanjappan, Satheeshkumar</au><au>Velayutham, Ravichandiran</au><au>Arumugam, Somasundaram</au><au>Agbor, Gabriel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of a natural compound extracted from Dolichandrone atrovirens as a novel antioxidant agent using Caenorhabditis elegans</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2021-09-22</date><risdate>2021</risdate><volume>16</volume><issue>9</issue><spage>e0257702</spage><pages>e0257702-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The compound methyl cinnamoyl catalpol (DAM-1) was isolated from the methanol extract of Dolichandrone atrovirens. Studies have already reported the antioxidant activity of Dolichandrone atrovirens bark extract, but till date the antioxidant activity of the isolated compound DAM-1, remains unexplored. The endogenous process of reactive oxygen species generation which leads to various degenerative diseases, can be broken down using these exogenous moieties from plant origin, herein this study we sought to evaluate the antioxidant potential of the DAM-1 compound using Caenorhabditis elegans (C. elegans), which is the primary model to study the antioxidant activity of compounds. Cytotoxicity assay results showed that DAM-1 treatment in the concentration of 10, 25 and 50 μg/ml has shown 100%, 91%, and 50% survival respectively with overall p<0.0001 (treatment v/s control group). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide-Formazan (MTT) assay results showed that treatment had better survival rates than the control group at different time intervals i.e. 48 h, and 72 h with p<0.01. Mechanosensation (behavioral study) as well as in vivo study results showed that at 0 h, 10 μg/ml of DAM-1 treatment showed a better anti-oxidative activity than the control group, 25 and 50 μg/ml of DAM-1 treated groups with p<0.001 but at 2.5 h incubation with 10, 25, 50 μg/ml of DAM-1 showed an increased anti-oxidative activity than the control group with p<0.001. Thermoresistance assay confirmed that the treatment group had more survival than control group with p<0.001. Absorption study of DAM-1 in C. elegans has shown that the absorption of the drug increases up to 180 mins with a slight decrease after 360 mins and then constant absorption up to 1440 mins. This study paves the way towards the initiative to explore the pharmacological role of DAM-1 in various oxidative stress mediated diseases at molecular levels and the absorption study points out its potential role which could be utilized in the metabolomics and proteomics analysis of this compound in other studies.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>34551009</pmid><doi>10.1371/journal.pone.0257702</doi><tpages>e0257702</tpages><orcidid>https://orcid.org/0000-0002-2096-2552</orcidid><orcidid>https://orcid.org/0000-0002-9627-6890</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2021-09, Vol.16 (9), p.e0257702 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2575314499 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Absorption Animals Antioxidants Antioxidants - chemistry Antioxidants - pharmacology Assaying Bark Biocompatibility Biological Products - chemistry Biological Products - pharmacology Biology and Life Sciences Bromides Caenorhabditis elegans Caenorhabditis elegans - drug effects Caenorhabditis elegans - metabolism Cytotoxicity Degenerative diseases Diabetes Dolichandrone Education Enzymes Food In vivo methods and tests Iridoid Glucosides - pharmacology Lamiales Mechanotransduction Medicine and Health Sciences Metabolites Metabolomics Natural products Oxidative stress Oxidative Stress - drug effects Oxygen Pharmaceutical sciences Pharmacology Phytochemicals Plant extracts Plant Extracts - chemistry Plant Extracts - pharmacology Properties Proteomics Reactive oxygen species Reactive Oxygen Species - metabolism Research and Analysis Methods Solvents Survival Toxicity Toxicology |
| title | Evaluation of a natural compound extracted from Dolichandrone atrovirens as a novel antioxidant agent using Caenorhabditis elegans |
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