Defective expansion and function of memory like natural killer cells in HIV+ individuals with latent tuberculosis infection
Tuberculosis (TB) is the leading cause of infectious disease related mortality, and only 10% of the infected individuals develop active disease. The likelihood of progression of latent tuberculosis infection (LTBI) to active TB disease is high in HIV infected individuals. Identification of HIV+ indi...
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| description | Tuberculosis (TB) is the leading cause of infectious disease related mortality, and only 10% of the infected individuals develop active disease. The likelihood of progression of latent tuberculosis infection (LTBI) to active TB disease is high in HIV infected individuals. Identification of HIV+ individuals at risk would allow treating targeted population, facilitating completion of therapy for LTBI and prevention of TB development. NK cells have an important role in T cell independent immunity against TB, but the exact role of NK cell subsets in LTBI and HIV is not well characterized. In this study, we compared the expansion and function of memory like NK cells from HIV-LTBI+ individuals and treatment naïve HIV+LTBI+ patients in response to Mtb antigens ESAT-6 and CFP-10. In freshly isolated PBMCs, percentages of CD3.sup.- CD56.sup.+ NK cells were similar in HIV+LTBI+ patients and healthy HIV-LTBI+ individuals. However, percentages of CD3.sup.- CD56.sup.+ CD16.sup.+ NK cells were higher in healthy HIV-LTBI+ individuals compared to HIV+LTBI+ patients. HIV infection also inhibited the expansion of memory like NK cells, production of IL-32[alpha], IL-15 and IFN-[gamma] in response to Mtb antigens in LTBI+ individuals. We studied phenotypic, functional subsets and activation of memory like-NK cells during HIV infection and LTBI. We observed that HIV+LTBI+ patients demonstrated suboptimal NK cell and monocyte interactions in response to Mtb, leading to reduced IL-15, IFN-[gamma] and granzyme B and increased CCL5 production. Our study highlights the effect of HIV and LTBI on modulation of NK cell activity to understand their role in development of interventions to prevent progression to TB in high risk individuals. |
| doi_str_mv | 10.1371/journal.pone.0257185 |
| format | Article |
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The likelihood of progression of latent tuberculosis infection (LTBI) to active TB disease is high in HIV infected individuals. Identification of HIV+ individuals at risk would allow treating targeted population, facilitating completion of therapy for LTBI and prevention of TB development. NK cells have an important role in T cell independent immunity against TB, but the exact role of NK cell subsets in LTBI and HIV is not well characterized. In this study, we compared the expansion and function of memory like NK cells from HIV-LTBI+ individuals and treatment naïve HIV+LTBI+ patients in response to Mtb antigens ESAT-6 and CFP-10. In freshly isolated PBMCs, percentages of CD3.sup.- CD56.sup.+ NK cells were similar in HIV+LTBI+ patients and healthy HIV-LTBI+ individuals. However, percentages of CD3.sup.- CD56.sup.+ CD16.sup.+ NK cells were higher in healthy HIV-LTBI+ individuals compared to HIV+LTBI+ patients. HIV infection also inhibited the expansion of memory like NK cells, production of IL-32[alpha], IL-15 and IFN-[gamma] in response to Mtb antigens in LTBI+ individuals. We studied phenotypic, functional subsets and activation of memory like-NK cells during HIV infection and LTBI. We observed that HIV+LTBI+ patients demonstrated suboptimal NK cell and monocyte interactions in response to Mtb, leading to reduced IL-15, IFN-[gamma] and granzyme B and increased CCL5 production. Our study highlights the effect of HIV and LTBI on modulation of NK cell activity to understand their role in development of interventions to prevent progression to TB in high risk individuals.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0257185</identifier><identifier>PMID: 34516566</identifier><language>eng</language><publisher>San Francisco: Public Library of Science</publisher><subject>Age groups ; Antigens ; Authorship ; Biology and life sciences ; Care and treatment ; CD16 antigen ; CD3 antigen ; CD56 antigen ; Cell activation ; Cytokines ; ESAT-6 antigen ; Expansion ; Granzyme B ; Health aspects ; Health care ; Health risks ; HIV ; HIV infection ; Human immunodeficiency virus ; Immune system ; Immunology ; Infections ; Infectious diseases ; Interleukin 15 ; Killer cells ; Lymphocytes ; Lymphocytes T ; Medical research ; Medicine and health sciences ; Molecular biology ; Monocytes ; Natural killer cells ; Pathogens ; Patients ; Public health ; Research and Analysis Methods ; Tuberculosis ; γ-Interferon</subject><ispartof>PloS one, 2021-09, Vol.16 (9), p.e0257185-e0257185</ispartof><rights>COPYRIGHT 2021 Public Library of Science</rights><rights>2021 Devalraju et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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expansion and function of memory like natural killer cells in HIV+ individuals with latent tuberculosis infection</title><author>Devalraju, Kamakshi Prudhula ; Neela, Venkata Sanjeev Kumar ; Krovvidi, Siva Sai ; Vankayalapati, Ramakrishna ; Valluri, Vijaya Lakshmi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5845-91a42dbf476d2a3bc8b216b912c0779ed1056d2a5b5b8cb5931dd9df7f6dd75a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Age groups</topic><topic>Antigens</topic><topic>Authorship</topic><topic>Biology and life sciences</topic><topic>Care and treatment</topic><topic>CD16 antigen</topic><topic>CD3 antigen</topic><topic>CD56 antigen</topic><topic>Cell activation</topic><topic>Cytokines</topic><topic>ESAT-6 antigen</topic><topic>Expansion</topic><topic>Granzyme B</topic><topic>Health aspects</topic><topic>Health care</topic><topic>Health 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infection</atitle><jtitle>PloS one</jtitle><date>2021-09-13</date><risdate>2021</risdate><volume>16</volume><issue>9</issue><spage>e0257185</spage><epage>e0257185</epage><pages>e0257185-e0257185</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Tuberculosis (TB) is the leading cause of infectious disease related mortality, and only 10% of the infected individuals develop active disease. The likelihood of progression of latent tuberculosis infection (LTBI) to active TB disease is high in HIV infected individuals. Identification of HIV+ individuals at risk would allow treating targeted population, facilitating completion of therapy for LTBI and prevention of TB development. NK cells have an important role in T cell independent immunity against TB, but the exact role of NK cell subsets in LTBI and HIV is not well characterized. In this study, we compared the expansion and function of memory like NK cells from HIV-LTBI+ individuals and treatment naïve HIV+LTBI+ patients in response to Mtb antigens ESAT-6 and CFP-10. In freshly isolated PBMCs, percentages of CD3.sup.- CD56.sup.+ NK cells were similar in HIV+LTBI+ patients and healthy HIV-LTBI+ individuals. However, percentages of CD3.sup.- CD56.sup.+ CD16.sup.+ NK cells were higher in healthy HIV-LTBI+ individuals compared to HIV+LTBI+ patients. HIV infection also inhibited the expansion of memory like NK cells, production of IL-32[alpha], IL-15 and IFN-[gamma] in response to Mtb antigens in LTBI+ individuals. We studied phenotypic, functional subsets and activation of memory like-NK cells during HIV infection and LTBI. We observed that HIV+LTBI+ patients demonstrated suboptimal NK cell and monocyte interactions in response to Mtb, leading to reduced IL-15, IFN-[gamma] and granzyme B and increased CCL5 production. Our study highlights the effect of HIV and LTBI on modulation of NK cell activity to understand their role in development of interventions to prevent progression to TB in high risk individuals.</abstract><cop>San Francisco</cop><pub>Public Library of Science</pub><pmid>34516566</pmid><doi>10.1371/journal.pone.0257185</doi><tpages>e0257185</tpages><orcidid>https://orcid.org/0000-0002-0462-4174</orcidid><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Age groups Antigens Authorship Biology and life sciences Care and treatment CD16 antigen CD3 antigen CD56 antigen Cell activation Cytokines ESAT-6 antigen Expansion Granzyme B Health aspects Health care Health risks HIV HIV infection Human immunodeficiency virus Immune system Immunology Infections Infectious diseases Interleukin 15 Killer cells Lymphocytes Lymphocytes T Medical research Medicine and health sciences Molecular biology Monocytes Natural killer cells Pathogens Patients Public health Research and Analysis Methods Tuberculosis γ-Interferon |
| title | Defective expansion and function of memory like natural killer cells in HIV+ individuals with latent tuberculosis infection |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T16%3A27%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Defective%20expansion%20and%20function%20of%20memory%20like%20natural%20killer%20cells%20in%20HIV+%20individuals%20with%20latent%20tuberculosis%20infection&rft.jtitle=PloS%20one&rft.au=Devalraju,%20Kamakshi%20Prudhula&rft.date=2021-09-13&rft.volume=16&rft.issue=9&rft.spage=e0257185&rft.epage=e0257185&rft.pages=e0257185-e0257185&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0257185&rft_dat=%3Cgale_plos_%3EA675239701%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2572202673&rft_id=info:pmid/34516566&rft_galeid=A675239701&rft_doaj_id=oai_doaj_org_article_d4f5d40d28274cb9a9e2d9cf2e3a48b5&rfr_iscdi=true |