Oligonucleotide capture sequencing of the SARS-CoV-2 genome and subgenomic fragments from COVID-19 individuals

The newly emerged and rapidly spreading SARS-CoV-2 causes coronavirus disease 2019 (COVID-19). To facilitate a deeper understanding of the viral biology we developed a capture sequencing methodology to generate SARS-CoV-2 genomic and transcriptome sequences from infected patients. We utilized an oli...

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Veröffentlicht in:	PloS one 2021-08, Vol.16 (8), p.e0244468-e0244468
Hauptverfasser:	Doddapaneni, Harsha, Cregeen, Sara Javornik, Sucgang, Richard, Meng, Qingchang, Qin, Xiang, Avadhanula, Vasanthi, Chao, Hsu, Menon, Vipin, Nicholson, Erin, Henke, David, Piedra, Felipe-Andres, Rajan, Anubama, Momin, Zeineen, Kottapalli, Kavya, Hoffman, Kristi L, Sedlazeck, Fritz J, Metcalf, Ginger, Piedra, Pedro A, Muzny, Donna M, Petrosino, Joseph F, Gibbs, Richard A
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Biology and life sciences Care and treatment Coronaviruses COVID-19 COVID-19 - pathology COVID-19 - virology Diagnosis DNA, Complementary - chemistry DNA, Complementary - metabolism Epidemics Gene Frequency Genetic transcription Genetic Variation Genome, Viral Genomes Genomics Humans Hybridization Medical schools Medicine Medicine and health sciences Oligonucleotides Open reading frames Open Reading Frames - genetics Patients Pediatrics Polymerase chain reaction Real-Time Polymerase Chain Reaction Research and analysis methods Ribonucleic acid RNA RNA, Viral - genetics RNA, Viral - metabolism SARS-CoV-2 - genetics SARS-CoV-2 - isolation & purification Sequence Analysis, DNA - methods Severe acute respiratory syndrome Severe acute respiratory syndrome coronavirus 2 Transcription Transcriptomes United States Viral diseases Viral Load Virology Workflow
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