Expression of tdTomato and luciferase in a murine lung cancer alters the growth and immune microenvironment of the tumor
Imaging techniques based on fluorescence and bioluminescence have been important tools in visualizing tumor progression and studying the effect of drugs and immunotherapies on tumor immune microenvironment in animal models of cancer. However, transgenic expression of foreign proteins may induce immu...
Gespeichert in:
| Veröffentlicht in: | PloS one 2021-08, Vol.16 (8), p.e0254125-e0254125 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0254125 |
|---|---|
| container_issue | 8 |
| container_start_page | e0254125 |
| container_title | PloS one |
| container_volume | 16 |
| creator | Huang, Lei Bommireddy, Ramireddy Munoz, Luis E Guin, Rohini N Wei, Changyong Ruggieri, Amanda Menon, Ashwathi P Li, Xiaoxian Shanmugam, Mala Owonikoko, Taofeek K Ramalingam, Suresh S Selvaraj, Periasamy |
| description | Imaging techniques based on fluorescence and bioluminescence have been important tools in visualizing tumor progression and studying the effect of drugs and immunotherapies on tumor immune microenvironment in animal models of cancer. However, transgenic expression of foreign proteins may induce immune responses in immunocompetent syngeneic tumor transplant models and augment the efficacy of experimental drugs. In this study, we show that the growth rate of Lewis lung carcinoma (LL/2) tumors was reduced after transduction of tdTomato and luciferase (tdTomato/Luc) compared to the parental cell line. tdTomato/Luc expression by LL/2 cells altered the tumor microenvironment by increasing tumor-infiltrating lymphocytes (TILs) while inhibiting tumor-induced myeloid-derived suppressor cells (MDSCs). Interestingly, tdTomato/Luc expression did not alter the response of LL/2 tumors to anti-PD-1 and anti-CTLA-4 antibodies. These results suggest that the use of tdTomato/Luc-transduced cancer cells to conduct studies in immune competent mice may lead to cell-extrinsic tdTomato/Luc-induced alterations in tumor growth and tumor immune microenvironment that need to be taken into consideration when evaluating the efficacy of anti-cancer drugs and vaccines in immunocompetent animal models. |
| doi_str_mv | 10.1371/journal.pone.0254125 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2562820683</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A672601062</galeid><doaj_id>oai_doaj_org_article_dc6e930ff2a44d15a2ac8e0dd7a119d8</doaj_id><sourcerecordid>A672601062</sourcerecordid><originalsourceid>FETCH-LOGICAL-c743t-67fe4216689ff1ab4df2df91588cafd4f40fc8934da9cedb183271720e9ce5483</originalsourceid><addsrcrecordid>eNqNk99v0zAQxyMEYmPwHyCIhITgocW_4iQvSNM0oNKkSTB4tVz73LpK7M52RvnvcdtsatEeUB6SXD73_ebOd0XxGqMppjX-tPJDcLKbrr2DKSIVw6R6UpzilpIJJ4g-PXg-KV7EuEKoog3nz4sTyhjGmLHTYnO5WQeI0XpXelMmfeN7mXwpnS67QVkDQUYorStl2Q_BOshhtyiVdApCKbsEIZZpCeUi-N9puUu0fT9ksLcqeHB3NnjXg0s7g0ymoffhZfHMyC7Cq_F-Vvz8cnlz8W1ydf11dnF-NVE1o2nCawOMYM6b1hgs50wbok2Lq6ZR0mhmGDKqaSnTslWg57ihpMY1QZBfK9bQs-LtXnfd-SjGpkVBKk4agnhDMzHbE9rLlVgH28vwR3hpxS7gw0LIkKzqQGjFoaXIGCIZ07iSRKoGkNa1xLjVW7fPo9sw70GrXHWQ3ZHo8Rdnl2Lh70RDa44QzgIfRoHgbweISfQ2Kug66cAPu_-mjDDSbr3e_YM-Xt1ILWQuwDrjs6_aiopzXhOOMOIkU9NHqHxpyKeYJ8zYHD9K-HiUkJkEm7SQQ4xi9uP7_7PXv47Z9wfsEvKALaPvhpQHNB6DbA_mGYsxgHloMkZiuyD33RDbBRHjguS0N4cH9JB0vxH0LzLqDOo</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2562820683</pqid></control><display><type>article</type><title>Expression of tdTomato and luciferase in a murine lung cancer alters the growth and immune microenvironment of the tumor</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>Public Library of Science (PLoS)</source><creator>Huang, Lei ; Bommireddy, Ramireddy ; Munoz, Luis E ; Guin, Rohini N ; Wei, Changyong ; Ruggieri, Amanda ; Menon, Ashwathi P ; Li, Xiaoxian ; Shanmugam, Mala ; Owonikoko, Taofeek K ; Ramalingam, Suresh S ; Selvaraj, Periasamy</creator><creatorcontrib>Huang, Lei ; Bommireddy, Ramireddy ; Munoz, Luis E ; Guin, Rohini N ; Wei, Changyong ; Ruggieri, Amanda ; Menon, Ashwathi P ; Li, Xiaoxian ; Shanmugam, Mala ; Owonikoko, Taofeek K ; Ramalingam, Suresh S ; Selvaraj, Periasamy</creatorcontrib><description>Imaging techniques based on fluorescence and bioluminescence have been important tools in visualizing tumor progression and studying the effect of drugs and immunotherapies on tumor immune microenvironment in animal models of cancer. However, transgenic expression of foreign proteins may induce immune responses in immunocompetent syngeneic tumor transplant models and augment the efficacy of experimental drugs. In this study, we show that the growth rate of Lewis lung carcinoma (LL/2) tumors was reduced after transduction of tdTomato and luciferase (tdTomato/Luc) compared to the parental cell line. tdTomato/Luc expression by LL/2 cells altered the tumor microenvironment by increasing tumor-infiltrating lymphocytes (TILs) while inhibiting tumor-induced myeloid-derived suppressor cells (MDSCs). Interestingly, tdTomato/Luc expression did not alter the response of LL/2 tumors to anti-PD-1 and anti-CTLA-4 antibodies. These results suggest that the use of tdTomato/Luc-transduced cancer cells to conduct studies in immune competent mice may lead to cell-extrinsic tdTomato/Luc-induced alterations in tumor growth and tumor immune microenvironment that need to be taken into consideration when evaluating the efficacy of anti-cancer drugs and vaccines in immunocompetent animal models.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0254125</identifier><identifier>PMID: 34411144</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animal models ; Animals ; Antibodies ; Anticancer properties ; Antitumor agents ; Biology and Life Sciences ; Bioluminescence ; Cancer cells ; Cancer vaccines ; Carcinoma, Lewis Lung - genetics ; Carcinoma, Lewis Lung - immunology ; Cell growth ; Cell Line, Tumor ; Cloning ; CTLA-4 protein ; Development and progression ; Diagnostic imaging ; Drugs ; Flow cytometry ; Fluorescence ; Funding ; Gene Expression ; Genes, Reporter - immunology ; Genetic aspects ; Genetic engineering ; Growth rate ; Health aspects ; Hematology ; Imaging techniques ; Immune response ; Immune system ; Immunosuppressive agents ; Immunotherapy ; Laboratories ; Luciferase ; Luciferases - genetics ; Luciferases - immunology ; Luminescent Proteins - genetics ; Luminescent Proteins - immunology ; Lung cancer ; Lung carcinoma ; Lung diseases ; Lung Neoplasms - genetics ; Lung Neoplasms - immunology ; Lymphocytes ; Medicine ; Medicine and Health Sciences ; Methods ; Mice ; Oncology ; Pathology ; PD-1 protein ; Penicillin ; Proteins ; Red Fluorescent Protein ; Research and Analysis Methods ; Suppressor cells ; Tumor microenvironment ; Tumor Microenvironment - genetics ; Tumor Microenvironment - immunology ; Tumor-infiltrating lymphocytes ; Tumors ; Vaccines</subject><ispartof>PloS one, 2021-08, Vol.16 (8), p.e0254125-e0254125</ispartof><rights>COPYRIGHT 2021 Public Library of Science</rights><rights>2021 Huang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 Huang et al 2021 Huang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c743t-67fe4216689ff1ab4df2df91588cafd4f40fc8934da9cedb183271720e9ce5483</citedby><cites>FETCH-LOGICAL-c743t-67fe4216689ff1ab4df2df91588cafd4f40fc8934da9cedb183271720e9ce5483</cites><orcidid>0000-0003-3130-0551 ; 0000-0002-4827-7282</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8376001/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8376001/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34411144$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Lei</creatorcontrib><creatorcontrib>Bommireddy, Ramireddy</creatorcontrib><creatorcontrib>Munoz, Luis E</creatorcontrib><creatorcontrib>Guin, Rohini N</creatorcontrib><creatorcontrib>Wei, Changyong</creatorcontrib><creatorcontrib>Ruggieri, Amanda</creatorcontrib><creatorcontrib>Menon, Ashwathi P</creatorcontrib><creatorcontrib>Li, Xiaoxian</creatorcontrib><creatorcontrib>Shanmugam, Mala</creatorcontrib><creatorcontrib>Owonikoko, Taofeek K</creatorcontrib><creatorcontrib>Ramalingam, Suresh S</creatorcontrib><creatorcontrib>Selvaraj, Periasamy</creatorcontrib><title>Expression of tdTomato and luciferase in a murine lung cancer alters the growth and immune microenvironment of the tumor</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Imaging techniques based on fluorescence and bioluminescence have been important tools in visualizing tumor progression and studying the effect of drugs and immunotherapies on tumor immune microenvironment in animal models of cancer. However, transgenic expression of foreign proteins may induce immune responses in immunocompetent syngeneic tumor transplant models and augment the efficacy of experimental drugs. In this study, we show that the growth rate of Lewis lung carcinoma (LL/2) tumors was reduced after transduction of tdTomato and luciferase (tdTomato/Luc) compared to the parental cell line. tdTomato/Luc expression by LL/2 cells altered the tumor microenvironment by increasing tumor-infiltrating lymphocytes (TILs) while inhibiting tumor-induced myeloid-derived suppressor cells (MDSCs). Interestingly, tdTomato/Luc expression did not alter the response of LL/2 tumors to anti-PD-1 and anti-CTLA-4 antibodies. These results suggest that the use of tdTomato/Luc-transduced cancer cells to conduct studies in immune competent mice may lead to cell-extrinsic tdTomato/Luc-induced alterations in tumor growth and tumor immune microenvironment that need to be taken into consideration when evaluating the efficacy of anti-cancer drugs and vaccines in immunocompetent animal models.</description><subject>Animal models</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Anticancer properties</subject><subject>Antitumor agents</subject><subject>Biology and Life Sciences</subject><subject>Bioluminescence</subject><subject>Cancer cells</subject><subject>Cancer vaccines</subject><subject>Carcinoma, Lewis Lung - genetics</subject><subject>Carcinoma, Lewis Lung - immunology</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cloning</subject><subject>CTLA-4 protein</subject><subject>Development and progression</subject><subject>Diagnostic imaging</subject><subject>Drugs</subject><subject>Flow cytometry</subject><subject>Fluorescence</subject><subject>Funding</subject><subject>Gene Expression</subject><subject>Genes, Reporter - immunology</subject><subject>Genetic aspects</subject><subject>Genetic engineering</subject><subject>Growth rate</subject><subject>Health aspects</subject><subject>Hematology</subject><subject>Imaging techniques</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Immunosuppressive agents</subject><subject>Immunotherapy</subject><subject>Laboratories</subject><subject>Luciferase</subject><subject>Luciferases - genetics</subject><subject>Luciferases - immunology</subject><subject>Luminescent Proteins - genetics</subject><subject>Luminescent Proteins - immunology</subject><subject>Lung cancer</subject><subject>Lung carcinoma</subject><subject>Lung diseases</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - immunology</subject><subject>Lymphocytes</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Methods</subject><subject>Mice</subject><subject>Oncology</subject><subject>Pathology</subject><subject>PD-1 protein</subject><subject>Penicillin</subject><subject>Proteins</subject><subject>Red Fluorescent Protein</subject><subject>Research and Analysis Methods</subject><subject>Suppressor cells</subject><subject>Tumor microenvironment</subject><subject>Tumor Microenvironment - genetics</subject><subject>Tumor Microenvironment - immunology</subject><subject>Tumor-infiltrating lymphocytes</subject><subject>Tumors</subject><subject>Vaccines</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk99v0zAQxyMEYmPwHyCIhITgocW_4iQvSNM0oNKkSTB4tVz73LpK7M52RvnvcdtsatEeUB6SXD73_ebOd0XxGqMppjX-tPJDcLKbrr2DKSIVw6R6UpzilpIJJ4g-PXg-KV7EuEKoog3nz4sTyhjGmLHTYnO5WQeI0XpXelMmfeN7mXwpnS67QVkDQUYorStl2Q_BOshhtyiVdApCKbsEIZZpCeUi-N9puUu0fT9ksLcqeHB3NnjXg0s7g0ymoffhZfHMyC7Cq_F-Vvz8cnlz8W1ydf11dnF-NVE1o2nCawOMYM6b1hgs50wbok2Lq6ZR0mhmGDKqaSnTslWg57ihpMY1QZBfK9bQs-LtXnfd-SjGpkVBKk4agnhDMzHbE9rLlVgH28vwR3hpxS7gw0LIkKzqQGjFoaXIGCIZ07iSRKoGkNa1xLjVW7fPo9sw70GrXHWQ3ZHo8Rdnl2Lh70RDa44QzgIfRoHgbweISfQ2Kug66cAPu_-mjDDSbr3e_YM-Xt1ILWQuwDrjs6_aiopzXhOOMOIkU9NHqHxpyKeYJ8zYHD9K-HiUkJkEm7SQQ4xi9uP7_7PXv47Z9wfsEvKALaPvhpQHNB6DbA_mGYsxgHloMkZiuyD33RDbBRHjguS0N4cH9JB0vxH0LzLqDOo</recordid><startdate>20210819</startdate><enddate>20210819</enddate><creator>Huang, Lei</creator><creator>Bommireddy, Ramireddy</creator><creator>Munoz, Luis E</creator><creator>Guin, Rohini N</creator><creator>Wei, Changyong</creator><creator>Ruggieri, Amanda</creator><creator>Menon, Ashwathi P</creator><creator>Li, Xiaoxian</creator><creator>Shanmugam, Mala</creator><creator>Owonikoko, Taofeek K</creator><creator>Ramalingam, Suresh S</creator><creator>Selvaraj, Periasamy</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-3130-0551</orcidid><orcidid>https://orcid.org/0000-0002-4827-7282</orcidid></search><sort><creationdate>20210819</creationdate><title>Expression of tdTomato and luciferase in a murine lung cancer alters the growth and immune microenvironment of the tumor</title><author>Huang, Lei ; Bommireddy, Ramireddy ; Munoz, Luis E ; Guin, Rohini N ; Wei, Changyong ; Ruggieri, Amanda ; Menon, Ashwathi P ; Li, Xiaoxian ; Shanmugam, Mala ; Owonikoko, Taofeek K ; Ramalingam, Suresh S ; Selvaraj, Periasamy</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c743t-67fe4216689ff1ab4df2df91588cafd4f40fc8934da9cedb183271720e9ce5483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animal models</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Anticancer properties</topic><topic>Antitumor agents</topic><topic>Biology and Life Sciences</topic><topic>Bioluminescence</topic><topic>Cancer cells</topic><topic>Cancer vaccines</topic><topic>Carcinoma, Lewis Lung - genetics</topic><topic>Carcinoma, Lewis Lung - immunology</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cloning</topic><topic>CTLA-4 protein</topic><topic>Development and progression</topic><topic>Diagnostic imaging</topic><topic>Drugs</topic><topic>Flow cytometry</topic><topic>Fluorescence</topic><topic>Funding</topic><topic>Gene Expression</topic><topic>Genes, Reporter - immunology</topic><topic>Genetic aspects</topic><topic>Genetic engineering</topic><topic>Growth rate</topic><topic>Health aspects</topic><topic>Hematology</topic><topic>Imaging techniques</topic><topic>Immune response</topic><topic>Immune system</topic><topic>Immunosuppressive agents</topic><topic>Immunotherapy</topic><topic>Laboratories</topic><topic>Luciferase</topic><topic>Luciferases - genetics</topic><topic>Luciferases - immunology</topic><topic>Luminescent Proteins - genetics</topic><topic>Luminescent Proteins - immunology</topic><topic>Lung cancer</topic><topic>Lung carcinoma</topic><topic>Lung diseases</topic><topic>Lung Neoplasms - genetics</topic><topic>Lung Neoplasms - immunology</topic><topic>Lymphocytes</topic><topic>Medicine</topic><topic>Medicine and Health Sciences</topic><topic>Methods</topic><topic>Mice</topic><topic>Oncology</topic><topic>Pathology</topic><topic>PD-1 protein</topic><topic>Penicillin</topic><topic>Proteins</topic><topic>Red Fluorescent Protein</topic><topic>Research and Analysis Methods</topic><topic>Suppressor cells</topic><topic>Tumor microenvironment</topic><topic>Tumor Microenvironment - genetics</topic><topic>Tumor Microenvironment - immunology</topic><topic>Tumor-infiltrating lymphocytes</topic><topic>Tumors</topic><topic>Vaccines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Lei</creatorcontrib><creatorcontrib>Bommireddy, Ramireddy</creatorcontrib><creatorcontrib>Munoz, Luis E</creatorcontrib><creatorcontrib>Guin, Rohini N</creatorcontrib><creatorcontrib>Wei, Changyong</creatorcontrib><creatorcontrib>Ruggieri, Amanda</creatorcontrib><creatorcontrib>Menon, Ashwathi P</creatorcontrib><creatorcontrib>Li, Xiaoxian</creatorcontrib><creatorcontrib>Shanmugam, Mala</creatorcontrib><creatorcontrib>Owonikoko, Taofeek K</creatorcontrib><creatorcontrib>Ramalingam, Suresh S</creatorcontrib><creatorcontrib>Selvaraj, Periasamy</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Lei</au><au>Bommireddy, Ramireddy</au><au>Munoz, Luis E</au><au>Guin, Rohini N</au><au>Wei, Changyong</au><au>Ruggieri, Amanda</au><au>Menon, Ashwathi P</au><au>Li, Xiaoxian</au><au>Shanmugam, Mala</au><au>Owonikoko, Taofeek K</au><au>Ramalingam, Suresh S</au><au>Selvaraj, Periasamy</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of tdTomato and luciferase in a murine lung cancer alters the growth and immune microenvironment of the tumor</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2021-08-19</date><risdate>2021</risdate><volume>16</volume><issue>8</issue><spage>e0254125</spage><epage>e0254125</epage><pages>e0254125-e0254125</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Imaging techniques based on fluorescence and bioluminescence have been important tools in visualizing tumor progression and studying the effect of drugs and immunotherapies on tumor immune microenvironment in animal models of cancer. However, transgenic expression of foreign proteins may induce immune responses in immunocompetent syngeneic tumor transplant models and augment the efficacy of experimental drugs. In this study, we show that the growth rate of Lewis lung carcinoma (LL/2) tumors was reduced after transduction of tdTomato and luciferase (tdTomato/Luc) compared to the parental cell line. tdTomato/Luc expression by LL/2 cells altered the tumor microenvironment by increasing tumor-infiltrating lymphocytes (TILs) while inhibiting tumor-induced myeloid-derived suppressor cells (MDSCs). Interestingly, tdTomato/Luc expression did not alter the response of LL/2 tumors to anti-PD-1 and anti-CTLA-4 antibodies. These results suggest that the use of tdTomato/Luc-transduced cancer cells to conduct studies in immune competent mice may lead to cell-extrinsic tdTomato/Luc-induced alterations in tumor growth and tumor immune microenvironment that need to be taken into consideration when evaluating the efficacy of anti-cancer drugs and vaccines in immunocompetent animal models.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>34411144</pmid><doi>10.1371/journal.pone.0254125</doi><tpages>e0254125</tpages><orcidid>https://orcid.org/0000-0003-3130-0551</orcidid><orcidid>https://orcid.org/0000-0002-4827-7282</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2021-08, Vol.16 (8), p.e0254125-e0254125 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2562820683 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Animal models Animals Antibodies Anticancer properties Antitumor agents Biology and Life Sciences Bioluminescence Cancer cells Cancer vaccines Carcinoma, Lewis Lung - genetics Carcinoma, Lewis Lung - immunology Cell growth Cell Line, Tumor Cloning CTLA-4 protein Development and progression Diagnostic imaging Drugs Flow cytometry Fluorescence Funding Gene Expression Genes, Reporter - immunology Genetic aspects Genetic engineering Growth rate Health aspects Hematology Imaging techniques Immune response Immune system Immunosuppressive agents Immunotherapy Laboratories Luciferase Luciferases - genetics Luciferases - immunology Luminescent Proteins - genetics Luminescent Proteins - immunology Lung cancer Lung carcinoma Lung diseases Lung Neoplasms - genetics Lung Neoplasms - immunology Lymphocytes Medicine Medicine and Health Sciences Methods Mice Oncology Pathology PD-1 protein Penicillin Proteins Red Fluorescent Protein Research and Analysis Methods Suppressor cells Tumor microenvironment Tumor Microenvironment - genetics Tumor Microenvironment - immunology Tumor-infiltrating lymphocytes Tumors Vaccines |
| title | Expression of tdTomato and luciferase in a murine lung cancer alters the growth and immune microenvironment of the tumor |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T23%3A12%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20of%20tdTomato%20and%20luciferase%20in%20a%20murine%20lung%20cancer%20alters%20the%20growth%20and%20immune%20microenvironment%20of%20the%20tumor&rft.jtitle=PloS%20one&rft.au=Huang,%20Lei&rft.date=2021-08-19&rft.volume=16&rft.issue=8&rft.spage=e0254125&rft.epage=e0254125&rft.pages=e0254125-e0254125&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0254125&rft_dat=%3Cgale_plos_%3EA672601062%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2562820683&rft_id=info:pmid/34411144&rft_galeid=A672601062&rft_doaj_id=oai_doaj_org_article_dc6e930ff2a44d15a2ac8e0dd7a119d8&rfr_iscdi=true |