An inexpensive point-of-care immunochromatographic test for Talaromyces marneffei infection based on the yeast phase specific monoclonal antibody 4D1 and Galanthus nivalis agglutinin

Talaromyces marneffei is a thermally dimorphic fungus that causes opportunistic systemic mycoses in patients with AIDS or other immunodeficiency syndromes. The purpose of this study was to develop an immunochromatographic strip test (ICT) based on a solid phase sandwich format immunoassay for the de...

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Veröffentlicht in:	PLoS neglected tropical diseases 2021-05, Vol.15 (5), p.e0009058-e0009058
Hauptverfasser:	Pruksaphon, Kritsada, Intaramat, Akarin, Simsiriwong, Pavinee, Mongkolsuk, Skorn, Ratanabanangkoon, Kavi, Nosanchuk, Joshua D, Kaltsas, Anna, Youngchim, Sirida
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Monoclonal - immunology Antigens Antigens, Fungal - urine Antigens, Surface - urine Beads Biology and Life Sciences Centrifugation Centrifuging Chromatography Diagnosis Dilution Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Ethics Fungal antigens Glass ballotini Gold Colloid - chemistry Health aspects Humans Identification and classification IgG antibody Immunoassay - methods Immunoglobulin G Incubation period Infections Laboratories Lectins Limit of Detection Mannose-Binding Lectin - immunology Mannose-Binding Lectins - immunology Medical sciences Medicine Medicine and Health Sciences Metal Nanoparticles - chemistry Methods Minimum inhibitory concentration Molecular diagnostic techniques Monoclonal antibodies Mycoses Mycoses - diagnosis Neglected Diseases - diagnosis Neglected Diseases - microbiology Pathogens Physical Sciences Plant Lectins - immunology Point-of-Care Testing Proteins Reaction plates Research and Analysis Methods Research ethics Room temperature Talaromyces - immunology Talaromyces - isolation & purification Therapy Tropical diseases Yeast Yeasts
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