Identification and evaluation of the inhibitory effect of Prunella vulgaris extract on SARS-coronavirus 2 virus entry
Until now, antiviral therapeutic agents are still urgently required for treatment or prevention of SARS-coronavirus 2 (SCoV-2) virus infection. In this study, we established a sensitive SCoV-2 Spike glycoprotein (SP), including an SP mutant D614G, pseudotyped HIV-1-based vector system and tested the...
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| creator | Ao, Zhujun Chan, Mable Ouyang, Maggie Jing Olukitibi, Titus Abiola Mahmoudi, Mona Kobasa, Darwyn Yao, Xiaojian |
| description | Until now, antiviral therapeutic agents are still urgently required for treatment or prevention of SARS-coronavirus 2 (SCoV-2) virus infection. In this study, we established a sensitive SCoV-2 Spike glycoprotein (SP), including an SP mutant D614G, pseudotyped HIV-1-based vector system and tested their ability to infect ACE2-expressing cells. Based on this system, we have demonstrated that an aqueous extract from the Natural herb Prunella vulgaris (NhPV) displayed potent inhibitory effects on SCoV-2 SP (including SP.sub.G614 mutant) pseudotyped virus (SCoV-2-SP-PVs) mediated infections. Moreover, we have compared NhPV with another compound, Suramin, for their anti-SARS-CoV-2 activities and the mode of their actions, and found that both NhPV and Suramin are able to directly interrupt SCoV-2-SP binding to its receptor ACE2 and block the viral entry step. Importantly, the inhibitory effects of NhPV and Suramin were confirmed by the wild type SARS-CoV-2 (hCoV-19/Canada/ON-VIDO-01/2020) virus infection in Vero cells. Furthermore, our results also demonstrated that the combination of NhPV/Suramin with an anti-SARS-CoV-2 neutralizing antibody mediated a more potent blocking effect against SCoV2-SP-PVs. Overall, by using SARS-CoV-2 SP-pseudotyped HIV-1-based entry system, we provide strong evidence that NhPV and Suramin have anti-SARS-CoV-2 activity and may be developed as a novel antiviral approach against SARS-CoV-2 infection. |
| doi_str_mv | 10.1371/journal.pone.0251649 |
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In this study, we established a sensitive SCoV-2 Spike glycoprotein (SP), including an SP mutant D614G, pseudotyped HIV-1-based vector system and tested their ability to infect ACE2-expressing cells. Based on this system, we have demonstrated that an aqueous extract from the Natural herb Prunella vulgaris (NhPV) displayed potent inhibitory effects on SCoV-2 SP (including SP.sub.G614 mutant) pseudotyped virus (SCoV-2-SP-PVs) mediated infections. Moreover, we have compared NhPV with another compound, Suramin, for their anti-SARS-CoV-2 activities and the mode of their actions, and found that both NhPV and Suramin are able to directly interrupt SCoV-2-SP binding to its receptor ACE2 and block the viral entry step. Importantly, the inhibitory effects of NhPV and Suramin were confirmed by the wild type SARS-CoV-2 (hCoV-19/Canada/ON-VIDO-01/2020) virus infection in Vero cells. Furthermore, our results also demonstrated that the combination of NhPV/Suramin with an anti-SARS-CoV-2 neutralizing antibody mediated a more potent blocking effect against SCoV2-SP-PVs. Overall, by using SARS-CoV-2 SP-pseudotyped HIV-1-based entry system, we provide strong evidence that NhPV and Suramin have anti-SARS-CoV-2 activity and may be developed as a novel antiviral approach against SARS-CoV-2 infection.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0251649</identifier><identifier>PMID: 34106944</identifier><language>eng</language><publisher>San Francisco: Public Library of Science</publisher><subject>Acquired immune deficiency syndrome ; AIDS ; Amino acids ; Anti-inflammatory agents ; Antibodies ; Antiviral agents ; Aspartic acid ; Biology and life sciences ; Cell culture ; Cellulose acetate ; Clinical trials ; Comparative analysis ; Coronaviruses ; COVID-19 ; Disease transmission ; Drugs ; Editing ; Fatalities ; Glycine ; Health sciences ; Hormones ; Hydroxychloroquine ; Infections ; Infectious diseases ; Inflammation ; Laboratories ; Load distribution ; Lopinavir ; Medical research ; Medical schools ; Medical treatment ; Medicine ; Medicine and health sciences ; Microbiology ; Middle East respiratory syndrome ; Mutation ; Pathogens ; Plasmids ; Public health ; R&D ; Research & development ; Research and Analysis Methods ; Respiratory diseases ; Reviews ; Severe acute respiratory syndrome ; Severe acute respiratory syndrome coronavirus 2 ; Suramin sodium ; Vaccines ; Viral diseases ; Viruses</subject><ispartof>PloS one, 2021-06, Vol.16 (6), p.e0251649-e0251649</ispartof><rights>COPYRIGHT 2021 Public Library of Science</rights><rights>2021 Ao et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Furthermore, our results also demonstrated that the combination of NhPV/Suramin with an anti-SARS-CoV-2 neutralizing antibody mediated a more potent blocking effect against SCoV2-SP-PVs. Overall, by using SARS-CoV-2 SP-pseudotyped HIV-1-based entry system, we provide strong evidence that NhPV and Suramin have anti-SARS-CoV-2 activity and may be developed as a novel antiviral approach against SARS-CoV-2 infection.</abstract><cop>San Francisco</cop><pub>Public Library of Science</pub><pmid>34106944</pmid><doi>10.1371/journal.pone.0251649</doi><tpages>e0251649</tpages><orcidid>https://orcid.org/0000-0002-3200-9638</orcidid><orcidid>https://orcid.org/0000-0002-5022-1309</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2021-06, Vol.16 (6), p.e0251649-e0251649 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2539515806 |
| source | DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acquired immune deficiency syndrome AIDS Amino acids Anti-inflammatory agents Antibodies Antiviral agents Aspartic acid Biology and life sciences Cell culture Cellulose acetate Clinical trials Comparative analysis Coronaviruses COVID-19 Disease transmission Drugs Editing Fatalities Glycine Health sciences Hormones Hydroxychloroquine Infections Infectious diseases Inflammation Laboratories Load distribution Lopinavir Medical research Medical schools Medical treatment Medicine Medicine and health sciences Microbiology Middle East respiratory syndrome Mutation Pathogens Plasmids Public health R&D Research & development Research and Analysis Methods Respiratory diseases Reviews Severe acute respiratory syndrome Severe acute respiratory syndrome coronavirus 2 Suramin sodium Vaccines Viral diseases Viruses |
| title | Identification and evaluation of the inhibitory effect of Prunella vulgaris extract on SARS-coronavirus 2 virus entry |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T12%3A10%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20and%20evaluation%20of%20the%20inhibitory%20effect%20of%20Prunella%20vulgaris%20extract%20on%20SARS-coronavirus%202%20virus%20entry&rft.jtitle=PloS%20one&rft.au=Ao,%20Zhujun&rft.date=2021-06-09&rft.volume=16&rft.issue=6&rft.spage=e0251649&rft.epage=e0251649&rft.pages=e0251649-e0251649&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0251649&rft_dat=%3Cgale_plos_%3EA664618725%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2539515806&rft_id=info:pmid/34106944&rft_galeid=A664618725&rft_doaj_id=oai_doaj_org_article_69f3ccca38b747f1a70e7651e39b53b7&rfr_iscdi=true |