Phylogenetic and evolutionary analysis of dengue virus serotypes circulating at the Colombian–Venezuelan border during 2015–2016 and 2018–2019
Dengue is an endemic disease in Colombia. Norte de Santander is a region on the border of Colombia and Venezuela and has reported the co-circulation and simultaneous co-infection of different serotypes of the dengue virus (DENV). This study aimed to conduct a phylogenetic analysis on the origin and...
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| description | Dengue is an endemic disease in Colombia. Norte de Santander is a region on the border of Colombia and Venezuela and has reported the co-circulation and simultaneous co-infection of different serotypes of the dengue virus (DENV). This study aimed to conduct a phylogenetic analysis on the origin and genetic diversity of DENV strains circulating in this bordering region. Serum samples were collected from patients who were clinically diagnosed with febrile syndrome associated with dengue during two periods. These samples were tested for DENV and serotyping was performed using reverse transcriptase-polymerase chain reaction. Subsequently, positive samples were amplified and the envelope protein gene of DENV was sequenced. Phylogenetic and phylogeographic analyses were performed using the sequences obtained. Basic local alignment search tool analysis confirmed that six and eight sequences belonged to DENV-1 and DENV-2, respectively. The phylogenetic analysis of DENV-1 showed that the sequences belonged to genotype V and clade I; they formed two groups: in the first group, two sequences showed a close phylogenetic relationship with strains from Ecuador and Panama, whereas the other four sequences were grouped with strains from Venezuela and Colombia. In the case of DENV-2, the analysis revealed that the sequences belonged to the Asian-American genotype and clade III. Furthermore, they formed two groups; in the first group, three sequences were grouped with strains from Colombia and Venezuela, whereas the other five were grouped with strains from Venezuela, Colombia and Honduras. This phylogenetic analysis suggests that the geographical proximity between Colombia and Venezuela is favourable for the export and import of different strains among serotypes or clades of the same DENV serotype, which could favour the spread of new outbreaks caused by new strains or genetic variants of this arbovirus. Therefore, this information highlights the importance of monitoring the transmission of DENV at border regions. |
| doi_str_mv | 10.1371/journal.pone.0252379 |
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Norte de Santander is a region on the border of Colombia and Venezuela and has reported the co-circulation and simultaneous co-infection of different serotypes of the dengue virus (DENV). This study aimed to conduct a phylogenetic analysis on the origin and genetic diversity of DENV strains circulating in this bordering region. Serum samples were collected from patients who were clinically diagnosed with febrile syndrome associated with dengue during two periods. These samples were tested for DENV and serotyping was performed using reverse transcriptase-polymerase chain reaction. Subsequently, positive samples were amplified and the envelope protein gene of DENV was sequenced. Phylogenetic and phylogeographic analyses were performed using the sequences obtained. Basic local alignment search tool analysis confirmed that six and eight sequences belonged to DENV-1 and DENV-2, respectively. The phylogenetic analysis of DENV-1 showed that the sequences belonged to genotype V and clade I; they formed two groups: in the first group, two sequences showed a close phylogenetic relationship with strains from Ecuador and Panama, whereas the other four sequences were grouped with strains from Venezuela and Colombia. In the case of DENV-2, the analysis revealed that the sequences belonged to the Asian-American genotype and clade III. Furthermore, they formed two groups; in the first group, three sequences were grouped with strains from Colombia and Venezuela, whereas the other five were grouped with strains from Venezuela, Colombia and Honduras. This phylogenetic analysis suggests that the geographical proximity between Colombia and Venezuela is favourable for the export and import of different strains among serotypes or clades of the same DENV serotype, which could favour the spread of new outbreaks caused by new strains or genetic variants of this arbovirus. Therefore, this information highlights the importance of monitoring the transmission of DENV at border regions.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0252379</identifier><identifier>PMID: 34048474</identifier><language>eng</language><publisher>San Francisco: Public Library of Science</publisher><subject>Analysis ; Biological diversity ; Biology and life sciences ; Computer and Information Sciences ; Dengue ; Dengue fever ; Dengue viruses ; DNA-directed RNA polymerase ; Earth Sciences ; Ecology and Environmental Sciences ; Epidemics ; Epidemiology ; Evolution ; Genetic aspects ; Genetic diversity ; Genetic variance ; Genomes ; Genotypes ; Geographical distribution ; Identification and classification ; Medicine and Health Sciences ; Migration ; Mutation ; People and places ; Phylogenetics ; Phylogeny ; Polarity ; Polymerase chain reaction ; Proteins ; Recombination ; Reverse transcription ; Ribonucleic acid ; RNA ; RNA polymerase ; Serotypes ; Structural proteins ; Vector-borne diseases ; Viral envelope proteins ; Viral infections ; Viruses</subject><ispartof>PloS one, 2021-05, Vol.16 (5), p.e0252379-e0252379</ispartof><rights>COPYRIGHT 2021 Public Library of Science</rights><rights>2021 Carrillo-Hernandez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 Carrillo-Hernandez et al 2021 Carrillo-Hernandez et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c669t-a2bf731415c26592b571c0479b2606e4219efc2da6b81a0794aba05d6ee2cdbc3</citedby><cites>FETCH-LOGICAL-c669t-a2bf731415c26592b571c0479b2606e4219efc2da6b81a0794aba05d6ee2cdbc3</cites><orcidid>0000-0002-9429-0058</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162668/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162668/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids></links><search><contributor>Zhang, Chiyu</contributor><creatorcontrib>Carrillo-Hernandez, Marlen Yelitza</creatorcontrib><creatorcontrib>Ruiz-Saenz, Julian</creatorcontrib><creatorcontrib>Jaimes-Villamizar, Lucy</creatorcontrib><creatorcontrib>Robledo-Restrepo, Sara Maria</creatorcontrib><creatorcontrib>Martinez-Gutierrez, Marlen</creatorcontrib><title>Phylogenetic and evolutionary analysis of dengue virus serotypes circulating at the Colombian–Venezuelan border during 2015–2016 and 2018–2019</title><title>PloS one</title><description>Dengue is an endemic disease in Colombia. Norte de Santander is a region on the border of Colombia and Venezuela and has reported the co-circulation and simultaneous co-infection of different serotypes of the dengue virus (DENV). This study aimed to conduct a phylogenetic analysis on the origin and genetic diversity of DENV strains circulating in this bordering region. Serum samples were collected from patients who were clinically diagnosed with febrile syndrome associated with dengue during two periods. These samples were tested for DENV and serotyping was performed using reverse transcriptase-polymerase chain reaction. Subsequently, positive samples were amplified and the envelope protein gene of DENV was sequenced. Phylogenetic and phylogeographic analyses were performed using the sequences obtained. Basic local alignment search tool analysis confirmed that six and eight sequences belonged to DENV-1 and DENV-2, respectively. The phylogenetic analysis of DENV-1 showed that the sequences belonged to genotype V and clade I; they formed two groups: in the first group, two sequences showed a close phylogenetic relationship with strains from Ecuador and Panama, whereas the other four sequences were grouped with strains from Venezuela and Colombia. In the case of DENV-2, the analysis revealed that the sequences belonged to the Asian-American genotype and clade III. Furthermore, they formed two groups; in the first group, three sequences were grouped with strains from Colombia and Venezuela, whereas the other five were grouped with strains from Venezuela, Colombia and Honduras. This phylogenetic analysis suggests that the geographical proximity between Colombia and Venezuela is favourable for the export and import of different strains among serotypes or clades of the same DENV serotype, which could favour the spread of new outbreaks caused by new strains or genetic variants of this arbovirus. Therefore, this information highlights the importance of monitoring the transmission of DENV at border regions.</description><subject>Analysis</subject><subject>Biological diversity</subject><subject>Biology and life sciences</subject><subject>Computer and Information Sciences</subject><subject>Dengue</subject><subject>Dengue fever</subject><subject>Dengue viruses</subject><subject>DNA-directed RNA polymerase</subject><subject>Earth Sciences</subject><subject>Ecology and Environmental Sciences</subject><subject>Epidemics</subject><subject>Epidemiology</subject><subject>Evolution</subject><subject>Genetic aspects</subject><subject>Genetic diversity</subject><subject>Genetic variance</subject><subject>Genomes</subject><subject>Genotypes</subject><subject>Geographical distribution</subject><subject>Identification and classification</subject><subject>Medicine and Health Sciences</subject><subject>Migration</subject><subject>Mutation</subject><subject>People and 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and evolutionary analysis of dengue virus serotypes circulating at the Colombian–Venezuelan border during 2015–2016 and 2018–2019</title><author>Carrillo-Hernandez, Marlen Yelitza ; Ruiz-Saenz, Julian ; Jaimes-Villamizar, Lucy ; Robledo-Restrepo, Sara Maria ; Martinez-Gutierrez, Marlen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c669t-a2bf731415c26592b571c0479b2606e4219efc2da6b81a0794aba05d6ee2cdbc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Analysis</topic><topic>Biological diversity</topic><topic>Biology and life sciences</topic><topic>Computer and Information Sciences</topic><topic>Dengue</topic><topic>Dengue fever</topic><topic>Dengue viruses</topic><topic>DNA-directed RNA polymerase</topic><topic>Earth Sciences</topic><topic>Ecology and Environmental Sciences</topic><topic>Epidemics</topic><topic>Epidemiology</topic><topic>Evolution</topic><topic>Genetic 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Chiyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phylogenetic and evolutionary analysis of dengue virus serotypes circulating at the Colombian–Venezuelan border during 2015–2016 and 2018–2019</atitle><jtitle>PloS one</jtitle><date>2021-05-28</date><risdate>2021</risdate><volume>16</volume><issue>5</issue><spage>e0252379</spage><epage>e0252379</epage><pages>e0252379-e0252379</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Dengue is an endemic disease in Colombia. Norte de Santander is a region on the border of Colombia and Venezuela and has reported the co-circulation and simultaneous co-infection of different serotypes of the dengue virus (DENV). This study aimed to conduct a phylogenetic analysis on the origin and genetic diversity of DENV strains circulating in this bordering region. Serum samples were collected from patients who were clinically diagnosed with febrile syndrome associated with dengue during two periods. These samples were tested for DENV and serotyping was performed using reverse transcriptase-polymerase chain reaction. Subsequently, positive samples were amplified and the envelope protein gene of DENV was sequenced. Phylogenetic and phylogeographic analyses were performed using the sequences obtained. Basic local alignment search tool analysis confirmed that six and eight sequences belonged to DENV-1 and DENV-2, respectively. The phylogenetic analysis of DENV-1 showed that the sequences belonged to genotype V and clade I; they formed two groups: in the first group, two sequences showed a close phylogenetic relationship with strains from Ecuador and Panama, whereas the other four sequences were grouped with strains from Venezuela and Colombia. In the case of DENV-2, the analysis revealed that the sequences belonged to the Asian-American genotype and clade III. Furthermore, they formed two groups; in the first group, three sequences were grouped with strains from Colombia and Venezuela, whereas the other five were grouped with strains from Venezuela, Colombia and Honduras. This phylogenetic analysis suggests that the geographical proximity between Colombia and Venezuela is favourable for the export and import of different strains among serotypes or clades of the same DENV serotype, which could favour the spread of new outbreaks caused by new strains or genetic variants of this arbovirus. Therefore, this information highlights the importance of monitoring the transmission of DENV at border regions.</abstract><cop>San Francisco</cop><pub>Public Library of Science</pub><pmid>34048474</pmid><doi>10.1371/journal.pone.0252379</doi><tpages>e0252379</tpages><orcidid>https://orcid.org/0000-0002-9429-0058</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Analysis Biological diversity Biology and life sciences Computer and Information Sciences Dengue Dengue fever Dengue viruses DNA-directed RNA polymerase Earth Sciences Ecology and Environmental Sciences Epidemics Epidemiology Evolution Genetic aspects Genetic diversity Genetic variance Genomes Genotypes Geographical distribution Identification and classification Medicine and Health Sciences Migration Mutation People and places Phylogenetics Phylogeny Polarity Polymerase chain reaction Proteins Recombination Reverse transcription Ribonucleic acid RNA RNA polymerase Serotypes Structural proteins Vector-borne diseases Viral envelope proteins Viral infections Viruses |
| title | Phylogenetic and evolutionary analysis of dengue virus serotypes circulating at the Colombian–Venezuelan border during 2015–2016 and 2018–2019 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T07%3A14%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Phylogenetic%20and%20evolutionary%20analysis%20of%20dengue%20virus%20serotypes%20circulating%20at%20the%20Colombian%E2%80%93Venezuelan%20border%20during%202015%E2%80%932016%20and%202018%E2%80%932019&rft.jtitle=PloS%20one&rft.au=Carrillo-Hernandez,%20Marlen%20Yelitza&rft.date=2021-05-28&rft.volume=16&rft.issue=5&rft.spage=e0252379&rft.epage=e0252379&rft.pages=e0252379-e0252379&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0252379&rft_dat=%3Cgale_plos_%3EA663442172%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2533696451&rft_id=info:pmid/34048474&rft_galeid=A663442172&rft_doaj_id=oai_doaj_org_article_114e1386b2f249c0948d6bb7194e45e4&rfr_iscdi=true |