Myosin 1C isoform A is a novel candidate diagnostic marker for prostate cancer
Early diagnosis of prostate cancer is a challenging issue due to the lack of specific markers. Therefore, a sensitive diagnostic marker that is expressed or upregulated exclusively in prostate cancer cells would facilitate diagnostic procedures and ensure a better outcome. We evaluated the expressio...
Gespeichert in:
| Veröffentlicht in: | PloS one 2021-05, Vol.16 (5), p.e0251961-e0251961 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0251961 |
|---|---|
| container_issue | 5 |
| container_start_page | e0251961 |
| container_title | PloS one |
| container_volume | 16 |
| creator | Saidova, Aleena A Potashnikova, Daria M Tvorogova, Anna V Paklina, Oxana V Veliev, Evgeniy I Knyshinsky, Grigoriy V Setdikova, Galiya R Rotin, Daniil L Maly, Ivan V Hofmann, Wilma A Vorobjev, Ivan A |
| description | Early diagnosis of prostate cancer is a challenging issue due to the lack of specific markers. Therefore, a sensitive diagnostic marker that is expressed or upregulated exclusively in prostate cancer cells would facilitate diagnostic procedures and ensure a better outcome. We evaluated the expression of myosin 1C isoform A in 5 prostate cell lines, 41 prostate cancer cases, and 11 benign hyperplasias. We analyzed the expression of 12 surface molecules on prostate cancer cells by flow cytometry and analyzed whether high or low myosin 1C isoform A expression could be attributed to a distinct phenotype of prostate cancer cells. Median myosin 1C isoform A expression in prostate cancer samples and cancer cell lines was 2 orders of magnitude higher than in benign prostate hyperplasia. Based on isoform A expression, we could also distinguish clinical stage 2 from clinical stage 3. Among cell lines, PC-3 cells with the highest myosin 1C isoform A level had diminished numbers of CD10/CD13-positive cells and increased numbers of CD29 (integrin [beta]1), CD38, CD54 (ICAM1) positive cells. The surface phenotype of clinical samples was similar to prostate cancer cell lines with high isoform A expression and could be described as CD10-/CD13- with heterogeneous expression of other markers. Both for cell lines and cancer specimens we observed the strong correlation of high myosin 1C isoform A mRNA expression and elevated levels of CD29 and CD54, suggesting a more adhesive phenotype for cells with high isoform A expression. Compared to normal tissue, prostate cancer samples had also reduced numbers of CD24- and CD38-positive cells. Our data suggest that a high level of myosin 1C isoform A is a specific marker both for prostate cancer cells and prostate cancer cell lines. High expression of isoform A is associated with less activated (CD24/CD38 low) and more adhesive (CD29/CD54 high) surface phenotype compared to benign prostate tissue. |
| doi_str_mv | 10.1371/journal.pone.0251961 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2530362640</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A662543333</galeid><doaj_id>oai_doaj_org_article_1b0c2d5660074b35b602de23710b0e47</doaj_id><sourcerecordid>A662543333</sourcerecordid><originalsourceid>FETCH-LOGICAL-c669t-cc55aad5ca7163ce5357eba45d498fc0d72fb72a1c019f6a4f69abb487e28e083</originalsourceid><addsrcrecordid>eNqNktuO0zAQhiMEYpfCGyARCQnBRYsPsZPcIFUVh0oLK3G6tSb2pHVJ7WInK_btcbcBbdBeYF_YGn_ze2b0Z9lTShaUl_T1zg_BQbc4eIcLwgStJb2XndOas7lkhN-_dT_LHsW4I0TwSsqH2RkvCK1Fzc-zTx-vfbQup6vcRt_6sM-X6ZZD7vwVdrkGZ6yBHnNjYeN87K3O9xB-YMgTnR9CCh2fE6gxPM4etNBFfDKes-zbu7dfVx_mF5fv16vlxVxLWfdzrYUAMEJDSSXXKLgosYFCmKKuWk1MydqmZEB1qrOVULSyhqYpqhJZhaTis-zZSffQ-ajGUUTFBCdcMlmQRKxPhPGwU4dgU9HXyoNVNwEfNgpCaqZDRRuimRFSElIWDReNJMwgS0MmDcGiTFpvxt-GZo9Go-sDdBPR6YuzW7XxV6qivBaUJYGXo0DwPweMvdrbqLHrwKEfbuqmjFLJaEKf_4Pe3d1IbSA1YF3r07_6KKqWUjJR8OOaZYs7qLQN7q1Ovmltik8SXk0SEtPjr34DQ4xq_eXz_7OX36fsi1vsFqHrt9F3Q2-9i1OwOIE6-SoGbP8OmRJ1tP2faaij7dVoe_4bZRDxbw</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2530362640</pqid></control><display><type>article</type><title>Myosin 1C isoform A is a novel candidate diagnostic marker for prostate cancer</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>Public Library of Science (PLoS)</source><creator>Saidova, Aleena A ; Potashnikova, Daria M ; Tvorogova, Anna V ; Paklina, Oxana V ; Veliev, Evgeniy I ; Knyshinsky, Grigoriy V ; Setdikova, Galiya R ; Rotin, Daniil L ; Maly, Ivan V ; Hofmann, Wilma A ; Vorobjev, Ivan A</creator><contributor>Saleem, Mohammad</contributor><creatorcontrib>Saidova, Aleena A ; Potashnikova, Daria M ; Tvorogova, Anna V ; Paklina, Oxana V ; Veliev, Evgeniy I ; Knyshinsky, Grigoriy V ; Setdikova, Galiya R ; Rotin, Daniil L ; Maly, Ivan V ; Hofmann, Wilma A ; Vorobjev, Ivan A ; Saleem, Mohammad</creatorcontrib><description>Early diagnosis of prostate cancer is a challenging issue due to the lack of specific markers. Therefore, a sensitive diagnostic marker that is expressed or upregulated exclusively in prostate cancer cells would facilitate diagnostic procedures and ensure a better outcome. We evaluated the expression of myosin 1C isoform A in 5 prostate cell lines, 41 prostate cancer cases, and 11 benign hyperplasias. We analyzed the expression of 12 surface molecules on prostate cancer cells by flow cytometry and analyzed whether high or low myosin 1C isoform A expression could be attributed to a distinct phenotype of prostate cancer cells. Median myosin 1C isoform A expression in prostate cancer samples and cancer cell lines was 2 orders of magnitude higher than in benign prostate hyperplasia. Based on isoform A expression, we could also distinguish clinical stage 2 from clinical stage 3. Among cell lines, PC-3 cells with the highest myosin 1C isoform A level had diminished numbers of CD10/CD13-positive cells and increased numbers of CD29 (integrin [beta]1), CD38, CD54 (ICAM1) positive cells. The surface phenotype of clinical samples was similar to prostate cancer cell lines with high isoform A expression and could be described as CD10-/CD13- with heterogeneous expression of other markers. Both for cell lines and cancer specimens we observed the strong correlation of high myosin 1C isoform A mRNA expression and elevated levels of CD29 and CD54, suggesting a more adhesive phenotype for cells with high isoform A expression. Compared to normal tissue, prostate cancer samples had also reduced numbers of CD24- and CD38-positive cells. Our data suggest that a high level of myosin 1C isoform A is a specific marker both for prostate cancer cells and prostate cancer cell lines. High expression of isoform A is associated with less activated (CD24/CD38 low) and more adhesive (CD29/CD54 high) surface phenotype compared to benign prostate tissue.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0251961</identifier><identifier>PMID: 34019593</identifier><language>eng</language><publisher>San Francisco: Public Library of Science</publisher><subject>Antibodies ; Biology ; Biology and Life Sciences ; Biophysics ; Biopsy ; Cell adhesion ; Cloning ; Committees ; Diagnosis ; Editing ; Epidermal growth factor ; Epithelial cells ; Epithelium ; Flow cytometry ; Gene expression ; Gentamicin ; Glutamine ; Growth factors ; Health aspects ; Histology ; Hyperplasia ; Medical diagnosis ; Medical research ; Medicine ; Medicine and Health Sciences ; Methodology ; mRNA ; Myosin ; Patients ; Physiology ; Pituitary ; Properties ; Prostate cancer ; Proteins ; Reviews ; Urology ; Visualization</subject><ispartof>PloS one, 2021-05, Vol.16 (5), p.e0251961-e0251961</ispartof><rights>COPYRIGHT 2021 Public Library of Science</rights><rights>2021 Saidova et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 Saidova et al 2021 Saidova et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c669t-cc55aad5ca7163ce5357eba45d498fc0d72fb72a1c019f6a4f69abb487e28e083</citedby><cites>FETCH-LOGICAL-c669t-cc55aad5ca7163ce5357eba45d498fc0d72fb72a1c019f6a4f69abb487e28e083</cites><orcidid>0000-0003-3294-7146</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139512/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139512/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23847,27903,27904,53769,53771,79346,79347</link.rule.ids></links><search><contributor>Saleem, Mohammad</contributor><creatorcontrib>Saidova, Aleena A</creatorcontrib><creatorcontrib>Potashnikova, Daria M</creatorcontrib><creatorcontrib>Tvorogova, Anna V</creatorcontrib><creatorcontrib>Paklina, Oxana V</creatorcontrib><creatorcontrib>Veliev, Evgeniy I</creatorcontrib><creatorcontrib>Knyshinsky, Grigoriy V</creatorcontrib><creatorcontrib>Setdikova, Galiya R</creatorcontrib><creatorcontrib>Rotin, Daniil L</creatorcontrib><creatorcontrib>Maly, Ivan V</creatorcontrib><creatorcontrib>Hofmann, Wilma A</creatorcontrib><creatorcontrib>Vorobjev, Ivan A</creatorcontrib><title>Myosin 1C isoform A is a novel candidate diagnostic marker for prostate cancer</title><title>PloS one</title><description>Early diagnosis of prostate cancer is a challenging issue due to the lack of specific markers. Therefore, a sensitive diagnostic marker that is expressed or upregulated exclusively in prostate cancer cells would facilitate diagnostic procedures and ensure a better outcome. We evaluated the expression of myosin 1C isoform A in 5 prostate cell lines, 41 prostate cancer cases, and 11 benign hyperplasias. We analyzed the expression of 12 surface molecules on prostate cancer cells by flow cytometry and analyzed whether high or low myosin 1C isoform A expression could be attributed to a distinct phenotype of prostate cancer cells. Median myosin 1C isoform A expression in prostate cancer samples and cancer cell lines was 2 orders of magnitude higher than in benign prostate hyperplasia. Based on isoform A expression, we could also distinguish clinical stage 2 from clinical stage 3. Among cell lines, PC-3 cells with the highest myosin 1C isoform A level had diminished numbers of CD10/CD13-positive cells and increased numbers of CD29 (integrin [beta]1), CD38, CD54 (ICAM1) positive cells. The surface phenotype of clinical samples was similar to prostate cancer cell lines with high isoform A expression and could be described as CD10-/CD13- with heterogeneous expression of other markers. Both for cell lines and cancer specimens we observed the strong correlation of high myosin 1C isoform A mRNA expression and elevated levels of CD29 and CD54, suggesting a more adhesive phenotype for cells with high isoform A expression. Compared to normal tissue, prostate cancer samples had also reduced numbers of CD24- and CD38-positive cells. Our data suggest that a high level of myosin 1C isoform A is a specific marker both for prostate cancer cells and prostate cancer cell lines. High expression of isoform A is associated with less activated (CD24/CD38 low) and more adhesive (CD29/CD54 high) surface phenotype compared to benign prostate tissue.</description><subject>Antibodies</subject><subject>Biology</subject><subject>Biology and Life Sciences</subject><subject>Biophysics</subject><subject>Biopsy</subject><subject>Cell adhesion</subject><subject>Cloning</subject><subject>Committees</subject><subject>Diagnosis</subject><subject>Editing</subject><subject>Epidermal growth factor</subject><subject>Epithelial cells</subject><subject>Epithelium</subject><subject>Flow cytometry</subject><subject>Gene expression</subject><subject>Gentamicin</subject><subject>Glutamine</subject><subject>Growth factors</subject><subject>Health aspects</subject><subject>Histology</subject><subject>Hyperplasia</subject><subject>Medical diagnosis</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Methodology</subject><subject>mRNA</subject><subject>Myosin</subject><subject>Patients</subject><subject>Physiology</subject><subject>Pituitary</subject><subject>Properties</subject><subject>Prostate cancer</subject><subject>Proteins</subject><subject>Reviews</subject><subject>Urology</subject><subject>Visualization</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNktuO0zAQhiMEYpfCGyARCQnBRYsPsZPcIFUVh0oLK3G6tSb2pHVJ7WInK_btcbcBbdBeYF_YGn_ze2b0Z9lTShaUl_T1zg_BQbc4eIcLwgStJb2XndOas7lkhN-_dT_LHsW4I0TwSsqH2RkvCK1Fzc-zTx-vfbQup6vcRt_6sM-X6ZZD7vwVdrkGZ6yBHnNjYeN87K3O9xB-YMgTnR9CCh2fE6gxPM4etNBFfDKes-zbu7dfVx_mF5fv16vlxVxLWfdzrYUAMEJDSSXXKLgosYFCmKKuWk1MydqmZEB1qrOVULSyhqYpqhJZhaTis-zZSffQ-ajGUUTFBCdcMlmQRKxPhPGwU4dgU9HXyoNVNwEfNgpCaqZDRRuimRFSElIWDReNJMwgS0MmDcGiTFpvxt-GZo9Go-sDdBPR6YuzW7XxV6qivBaUJYGXo0DwPweMvdrbqLHrwKEfbuqmjFLJaEKf_4Pe3d1IbSA1YF3r07_6KKqWUjJR8OOaZYs7qLQN7q1Ovmltik8SXk0SEtPjr34DQ4xq_eXz_7OX36fsi1vsFqHrt9F3Q2-9i1OwOIE6-SoGbP8OmRJ1tP2faaij7dVoe_4bZRDxbw</recordid><startdate>20210521</startdate><enddate>20210521</enddate><creator>Saidova, Aleena A</creator><creator>Potashnikova, Daria M</creator><creator>Tvorogova, Anna V</creator><creator>Paklina, Oxana V</creator><creator>Veliev, Evgeniy I</creator><creator>Knyshinsky, Grigoriy V</creator><creator>Setdikova, Galiya R</creator><creator>Rotin, Daniil L</creator><creator>Maly, Ivan V</creator><creator>Hofmann, Wilma A</creator><creator>Vorobjev, Ivan A</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-3294-7146</orcidid></search><sort><creationdate>20210521</creationdate><title>Myosin 1C isoform A is a novel candidate diagnostic marker for prostate cancer</title><author>Saidova, Aleena A ; Potashnikova, Daria M ; Tvorogova, Anna V ; Paklina, Oxana V ; Veliev, Evgeniy I ; Knyshinsky, Grigoriy V ; Setdikova, Galiya R ; Rotin, Daniil L ; Maly, Ivan V ; Hofmann, Wilma A ; Vorobjev, Ivan A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c669t-cc55aad5ca7163ce5357eba45d498fc0d72fb72a1c019f6a4f69abb487e28e083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Antibodies</topic><topic>Biology</topic><topic>Biology and Life Sciences</topic><topic>Biophysics</topic><topic>Biopsy</topic><topic>Cell adhesion</topic><topic>Cloning</topic><topic>Committees</topic><topic>Diagnosis</topic><topic>Editing</topic><topic>Epidermal growth factor</topic><topic>Epithelial cells</topic><topic>Epithelium</topic><topic>Flow cytometry</topic><topic>Gene expression</topic><topic>Gentamicin</topic><topic>Glutamine</topic><topic>Growth factors</topic><topic>Health aspects</topic><topic>Histology</topic><topic>Hyperplasia</topic><topic>Medical diagnosis</topic><topic>Medical research</topic><topic>Medicine</topic><topic>Medicine and Health Sciences</topic><topic>Methodology</topic><topic>mRNA</topic><topic>Myosin</topic><topic>Patients</topic><topic>Physiology</topic><topic>Pituitary</topic><topic>Properties</topic><topic>Prostate cancer</topic><topic>Proteins</topic><topic>Reviews</topic><topic>Urology</topic><topic>Visualization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saidova, Aleena A</creatorcontrib><creatorcontrib>Potashnikova, Daria M</creatorcontrib><creatorcontrib>Tvorogova, Anna V</creatorcontrib><creatorcontrib>Paklina, Oxana V</creatorcontrib><creatorcontrib>Veliev, Evgeniy I</creatorcontrib><creatorcontrib>Knyshinsky, Grigoriy V</creatorcontrib><creatorcontrib>Setdikova, Galiya R</creatorcontrib><creatorcontrib>Rotin, Daniil L</creatorcontrib><creatorcontrib>Maly, Ivan V</creatorcontrib><creatorcontrib>Hofmann, Wilma A</creatorcontrib><creatorcontrib>Vorobjev, Ivan A</creatorcontrib><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saidova, Aleena A</au><au>Potashnikova, Daria M</au><au>Tvorogova, Anna V</au><au>Paklina, Oxana V</au><au>Veliev, Evgeniy I</au><au>Knyshinsky, Grigoriy V</au><au>Setdikova, Galiya R</au><au>Rotin, Daniil L</au><au>Maly, Ivan V</au><au>Hofmann, Wilma A</au><au>Vorobjev, Ivan A</au><au>Saleem, Mohammad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Myosin 1C isoform A is a novel candidate diagnostic marker for prostate cancer</atitle><jtitle>PloS one</jtitle><date>2021-05-21</date><risdate>2021</risdate><volume>16</volume><issue>5</issue><spage>e0251961</spage><epage>e0251961</epage><pages>e0251961-e0251961</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Early diagnosis of prostate cancer is a challenging issue due to the lack of specific markers. Therefore, a sensitive diagnostic marker that is expressed or upregulated exclusively in prostate cancer cells would facilitate diagnostic procedures and ensure a better outcome. We evaluated the expression of myosin 1C isoform A in 5 prostate cell lines, 41 prostate cancer cases, and 11 benign hyperplasias. We analyzed the expression of 12 surface molecules on prostate cancer cells by flow cytometry and analyzed whether high or low myosin 1C isoform A expression could be attributed to a distinct phenotype of prostate cancer cells. Median myosin 1C isoform A expression in prostate cancer samples and cancer cell lines was 2 orders of magnitude higher than in benign prostate hyperplasia. Based on isoform A expression, we could also distinguish clinical stage 2 from clinical stage 3. Among cell lines, PC-3 cells with the highest myosin 1C isoform A level had diminished numbers of CD10/CD13-positive cells and increased numbers of CD29 (integrin [beta]1), CD38, CD54 (ICAM1) positive cells. The surface phenotype of clinical samples was similar to prostate cancer cell lines with high isoform A expression and could be described as CD10-/CD13- with heterogeneous expression of other markers. Both for cell lines and cancer specimens we observed the strong correlation of high myosin 1C isoform A mRNA expression and elevated levels of CD29 and CD54, suggesting a more adhesive phenotype for cells with high isoform A expression. Compared to normal tissue, prostate cancer samples had also reduced numbers of CD24- and CD38-positive cells. Our data suggest that a high level of myosin 1C isoform A is a specific marker both for prostate cancer cells and prostate cancer cell lines. High expression of isoform A is associated with less activated (CD24/CD38 low) and more adhesive (CD29/CD54 high) surface phenotype compared to benign prostate tissue.</abstract><cop>San Francisco</cop><pub>Public Library of Science</pub><pmid>34019593</pmid><doi>10.1371/journal.pone.0251961</doi><tpages>e0251961</tpages><orcidid>https://orcid.org/0000-0003-3294-7146</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2021-05, Vol.16 (5), p.e0251961-e0251961 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2530362640 |
| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Antibodies Biology Biology and Life Sciences Biophysics Biopsy Cell adhesion Cloning Committees Diagnosis Editing Epidermal growth factor Epithelial cells Epithelium Flow cytometry Gene expression Gentamicin Glutamine Growth factors Health aspects Histology Hyperplasia Medical diagnosis Medical research Medicine Medicine and Health Sciences Methodology mRNA Myosin Patients Physiology Pituitary Properties Prostate cancer Proteins Reviews Urology Visualization |
| title | Myosin 1C isoform A is a novel candidate diagnostic marker for prostate cancer |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T17%3A49%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Myosin%201C%20isoform%20A%20is%20a%20novel%20candidate%20diagnostic%20marker%20for%20prostate%20cancer&rft.jtitle=PloS%20one&rft.au=Saidova,%20Aleena%20A&rft.date=2021-05-21&rft.volume=16&rft.issue=5&rft.spage=e0251961&rft.epage=e0251961&rft.pages=e0251961-e0251961&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0251961&rft_dat=%3Cgale_plos_%3EA662543333%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2530362640&rft_id=info:pmid/34019593&rft_galeid=A662543333&rft_doaj_id=oai_doaj_org_article_1b0c2d5660074b35b602de23710b0e47&rfr_iscdi=true |