β-lactam resistance associated with β-lactamase production and porin alteration in clinical isolates of E. coli and K. pneumoniae
β-lactam resistance represents a worldwide problem and a serious challenge for antimicrobial treatment. Hence this research was conducted to recognize several mechanisms mediating β-lactam resistance in E. coli and K. pneumoniae clinical isolates collected from Mansoura University hospitals, Egypt....
Gespeichert in:
| Veröffentlicht in: | PloS one 2021-05, Vol.16 (5), p.e0251594-e0251594 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0251594 |
|---|---|
| container_issue | 5 |
| container_start_page | e0251594 |
| container_title | PloS one |
| container_volume | 16 |
| creator | Khalifa, Sara M Abd El-Aziz, Abeer M Hassan, Ramadan Abdelmegeed, Eman S |
| description | β-lactam resistance represents a worldwide problem and a serious challenge for antimicrobial treatment. Hence this research was conducted to recognize several mechanisms mediating β-lactam resistance in E. coli and K. pneumoniae clinical isolates collected from Mansoura University hospitals, Egypt. A total of 80 isolates, 45 E. coli and 35 K. pneumoniae isolates, were collected and their antibiotic susceptibility was determined by the Disc diffusion method followed by phenotypic and genotypic detection of extended-spectrum β-lactamases (ESBLs), AmpC β-lactamase, carbapenemase enzymes. The outer membrane protein porins of all isolates were analyzed and their genes were examined using gene amplification and sequencing. Also, the resistance to complement-mediated serum killing was estimated. A significant percentage of isolates (93.8%) were multidrug resistance and showed an elevated resistance to β-lactam antibiotics. The presence of either ESBL or AmpC enzymes was high among isolates (83.75%). Also, 60% of the isolated strains were carbapenemase producers. The most frequently detected gene of ESBL among all tested isolates was blaCTX-M-15 (86.3%) followed by blaTEM-1 (81.3%) and blaSHV-1 (35%) while the Amp-C gene was present in 83.75%. For carbapenemase-producing isolates, blaNDM1 was the most common (60%) followed by blaVIM-1 (35%) and blaOXA-48 (13.8%). Besides, 73.3% and 40% of E. coli and K. pneumoniae isolates respectively were serum resistant. Outer membrane protein analysis showed that 93.3% of E. coli and 95.7% of K. pneumoniae isolates lost their porins or showed modified porins. Furthermore, sequence analysis of tested porin genes in some isolates revealed the presence of frameshift mutations that produced truncated proteins of smaller size. β-lactam resistance in K. pneumoniae and E. coli isolates in our hospitals is due to a combination of β-lactamase activity and porin loss/alteration. Hence more restrictions should be applied on β-lactams usage to decrease the emergence of resistant strains. |
| doi_str_mv | 10.1371/journal.pone.0251594 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_plos_</sourceid><recordid>TN_cdi_plos_journals_2529909165</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_4627f6ef30c245478378253de53e845e</doaj_id><sourcerecordid>2529909165</sourcerecordid><originalsourceid>FETCH-LOGICAL-c526t-3bbd978a4e3bf234f583f74bf759507b3d41f7575db2ccae26212c55dc004ec53</originalsourceid><addsrcrecordid>eNptUstu1DAUjRCIlsIfILDEhk2C3042SKgqUFGJDawtx75pPfLYwU5ArPkjPoRvIjOTjlrExj6-Puf4XutU1XOCG8IUebNJc44mNGOK0GAqiOj4g-qUdIzWkmL28A4-qZ6UssFYsFbKx9UJ45jwTqjT6tef33UwdjJblKH4MploAZlSkvVmAod--OkGHUmmABpzcrOdfIrIRIfGlP2CwgTZ7IvLyQYfvTUB-ZLCYlNQGtBFg2wKfi_61KAxwrxN0Rt4Wj0aTCjwbN3Pqq_vL76cf6yvPn-4PH93VVtB5VSzvnedag0H1g-U8UG0bFC8H5ToBFY9c5wsWAnXU2sNUEkJtUI4izEHK9hZ9fLgO4ZU9Pp_RVNBuw53RO4YlweGS2ajx-y3Jv_UyXi9L6R8rU2evA2guaRqkDAwbCkXXLVMtVQwB4JBywUsXm_X1-Z-C85CnLIJ90zv30R_o6_Td90SJhXrFoPXq0FO32Yok976YiEEEyHNu74ZoVQuy0J99Q_1_9PxA8vmVEqG4dgMwXqXqVuV3mVKr5laZC_uDnIU3YaI_QULaszo</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2529909165</pqid></control><display><type>article</type><title>β-lactam resistance associated with β-lactamase production and porin alteration in clinical isolates of E. coli and K. pneumoniae</title><source>Public Library of Science (PLoS) Journals Open Access</source><source>Free E-Journal (出版社公開部分のみ）</source><source>DOAJ Directory of Open Access Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Khalifa, Sara M ; Abd El-Aziz, Abeer M ; Hassan, Ramadan ; Abdelmegeed, Eman S</creator><contributor>Karunasagar, Iddya</contributor><creatorcontrib>Khalifa, Sara M ; Abd El-Aziz, Abeer M ; Hassan, Ramadan ; Abdelmegeed, Eman S ; Karunasagar, Iddya</creatorcontrib><description>β-lactam resistance represents a worldwide problem and a serious challenge for antimicrobial treatment. Hence this research was conducted to recognize several mechanisms mediating β-lactam resistance in E. coli and K. pneumoniae clinical isolates collected from Mansoura University hospitals, Egypt. A total of 80 isolates, 45 E. coli and 35 K. pneumoniae isolates, were collected and their antibiotic susceptibility was determined by the Disc diffusion method followed by phenotypic and genotypic detection of extended-spectrum β-lactamases (ESBLs), AmpC β-lactamase, carbapenemase enzymes. The outer membrane protein porins of all isolates were analyzed and their genes were examined using gene amplification and sequencing. Also, the resistance to complement-mediated serum killing was estimated. A significant percentage of isolates (93.8%) were multidrug resistance and showed an elevated resistance to β-lactam antibiotics. The presence of either ESBL or AmpC enzymes was high among isolates (83.75%). Also, 60% of the isolated strains were carbapenemase producers. The most frequently detected gene of ESBL among all tested isolates was blaCTX-M-15 (86.3%) followed by blaTEM-1 (81.3%) and blaSHV-1 (35%) while the Amp-C gene was present in 83.75%. For carbapenemase-producing isolates, blaNDM1 was the most common (60%) followed by blaVIM-1 (35%) and blaOXA-48 (13.8%). Besides, 73.3% and 40% of E. coli and K. pneumoniae isolates respectively were serum resistant. Outer membrane protein analysis showed that 93.3% of E. coli and 95.7% of K. pneumoniae isolates lost their porins or showed modified porins. Furthermore, sequence analysis of tested porin genes in some isolates revealed the presence of frameshift mutations that produced truncated proteins of smaller size. β-lactam resistance in K. pneumoniae and E. coli isolates in our hospitals is due to a combination of β-lactamase activity and porin loss/alteration. Hence more restrictions should be applied on β-lactams usage to decrease the emergence of resistant strains.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0251594</identifier><identifier>PMID: 34014957</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Amides ; Antibiotics ; Antimicrobial agents ; Bacilli ; Bacteria ; Biology and Life Sciences ; Clinical isolates ; E coli ; Efflux ; Enzymes ; Gene amplification ; Gram-negative bacilli ; Gram-negative bacteria ; Immunology ; Laboratories ; Medicine and Health Sciences ; Membrane permeability ; Microbiology ; Mutation ; Penicillin ; People and Places ; Permeability ; Pharmacy ; Proteins ; Research and Analysis Methods ; Strains (organisms) ; Therapeutic targets ; β Lactamase ; β-Lactam antibiotics</subject><ispartof>PloS one, 2021-05, Vol.16 (5), p.e0251594-e0251594</ispartof><rights>2021 Khalifa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 Khalifa et al 2021 Khalifa et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-3bbd978a4e3bf234f583f74bf759507b3d41f7575db2ccae26212c55dc004ec53</citedby><cites>FETCH-LOGICAL-c526t-3bbd978a4e3bf234f583f74bf759507b3d41f7575db2ccae26212c55dc004ec53</cites><orcidid>0000-0002-6214-5091</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8136739/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8136739/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34014957$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Karunasagar, Iddya</contributor><creatorcontrib>Khalifa, Sara M</creatorcontrib><creatorcontrib>Abd El-Aziz, Abeer M</creatorcontrib><creatorcontrib>Hassan, Ramadan</creatorcontrib><creatorcontrib>Abdelmegeed, Eman S</creatorcontrib><title>β-lactam resistance associated with β-lactamase production and porin alteration in clinical isolates of E. coli and K. pneumoniae</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>β-lactam resistance represents a worldwide problem and a serious challenge for antimicrobial treatment. Hence this research was conducted to recognize several mechanisms mediating β-lactam resistance in E. coli and K. pneumoniae clinical isolates collected from Mansoura University hospitals, Egypt. A total of 80 isolates, 45 E. coli and 35 K. pneumoniae isolates, were collected and their antibiotic susceptibility was determined by the Disc diffusion method followed by phenotypic and genotypic detection of extended-spectrum β-lactamases (ESBLs), AmpC β-lactamase, carbapenemase enzymes. The outer membrane protein porins of all isolates were analyzed and their genes were examined using gene amplification and sequencing. Also, the resistance to complement-mediated serum killing was estimated. A significant percentage of isolates (93.8%) were multidrug resistance and showed an elevated resistance to β-lactam antibiotics. The presence of either ESBL or AmpC enzymes was high among isolates (83.75%). Also, 60% of the isolated strains were carbapenemase producers. The most frequently detected gene of ESBL among all tested isolates was blaCTX-M-15 (86.3%) followed by blaTEM-1 (81.3%) and blaSHV-1 (35%) while the Amp-C gene was present in 83.75%. For carbapenemase-producing isolates, blaNDM1 was the most common (60%) followed by blaVIM-1 (35%) and blaOXA-48 (13.8%). Besides, 73.3% and 40% of E. coli and K. pneumoniae isolates respectively were serum resistant. Outer membrane protein analysis showed that 93.3% of E. coli and 95.7% of K. pneumoniae isolates lost their porins or showed modified porins. Furthermore, sequence analysis of tested porin genes in some isolates revealed the presence of frameshift mutations that produced truncated proteins of smaller size. β-lactam resistance in K. pneumoniae and E. coli isolates in our hospitals is due to a combination of β-lactamase activity and porin loss/alteration. Hence more restrictions should be applied on β-lactams usage to decrease the emergence of resistant strains.</description><subject>Amides</subject><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Bacilli</subject><subject>Bacteria</subject><subject>Biology and Life Sciences</subject><subject>Clinical isolates</subject><subject>E coli</subject><subject>Efflux</subject><subject>Enzymes</subject><subject>Gene amplification</subject><subject>Gram-negative bacilli</subject><subject>Gram-negative bacteria</subject><subject>Immunology</subject><subject>Laboratories</subject><subject>Medicine and Health Sciences</subject><subject>Membrane permeability</subject><subject>Microbiology</subject><subject>Mutation</subject><subject>Penicillin</subject><subject>People and Places</subject><subject>Permeability</subject><subject>Pharmacy</subject><subject>Proteins</subject><subject>Research and Analysis Methods</subject><subject>Strains (organisms)</subject><subject>Therapeutic targets</subject><subject>β Lactamase</subject><subject>β-Lactam antibiotics</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptUstu1DAUjRCIlsIfILDEhk2C3042SKgqUFGJDawtx75pPfLYwU5ArPkjPoRvIjOTjlrExj6-Puf4XutU1XOCG8IUebNJc44mNGOK0GAqiOj4g-qUdIzWkmL28A4-qZ6UssFYsFbKx9UJ45jwTqjT6tef33UwdjJblKH4MploAZlSkvVmAod--OkGHUmmABpzcrOdfIrIRIfGlP2CwgTZ7IvLyQYfvTUB-ZLCYlNQGtBFg2wKfi_61KAxwrxN0Rt4Wj0aTCjwbN3Pqq_vL76cf6yvPn-4PH93VVtB5VSzvnedag0H1g-U8UG0bFC8H5ToBFY9c5wsWAnXU2sNUEkJtUI4izEHK9hZ9fLgO4ZU9Pp_RVNBuw53RO4YlweGS2ajx-y3Jv_UyXi9L6R8rU2evA2guaRqkDAwbCkXXLVMtVQwB4JBywUsXm_X1-Z-C85CnLIJ90zv30R_o6_Td90SJhXrFoPXq0FO32Yok976YiEEEyHNu74ZoVQuy0J99Q_1_9PxA8vmVEqG4dgMwXqXqVuV3mVKr5laZC_uDnIU3YaI_QULaszo</recordid><startdate>20210520</startdate><enddate>20210520</enddate><creator>Khalifa, Sara M</creator><creator>Abd El-Aziz, Abeer M</creator><creator>Hassan, Ramadan</creator><creator>Abdelmegeed, Eman S</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-6214-5091</orcidid></search><sort><creationdate>20210520</creationdate><title>β-lactam resistance associated with β-lactamase production and porin alteration in clinical isolates of E. coli and K. pneumoniae</title><author>Khalifa, Sara M ; Abd El-Aziz, Abeer M ; Hassan, Ramadan ; Abdelmegeed, Eman S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-3bbd978a4e3bf234f583f74bf759507b3d41f7575db2ccae26212c55dc004ec53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Amides</topic><topic>Antibiotics</topic><topic>Antimicrobial agents</topic><topic>Bacilli</topic><topic>Bacteria</topic><topic>Biology and Life Sciences</topic><topic>Clinical isolates</topic><topic>E coli</topic><topic>Efflux</topic><topic>Enzymes</topic><topic>Gene amplification</topic><topic>Gram-negative bacilli</topic><topic>Gram-negative bacteria</topic><topic>Immunology</topic><topic>Laboratories</topic><topic>Medicine and Health Sciences</topic><topic>Membrane permeability</topic><topic>Microbiology</topic><topic>Mutation</topic><topic>Penicillin</topic><topic>People and Places</topic><topic>Permeability</topic><topic>Pharmacy</topic><topic>Proteins</topic><topic>Research and Analysis Methods</topic><topic>Strains (organisms)</topic><topic>Therapeutic targets</topic><topic>β Lactamase</topic><topic>β-Lactam antibiotics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khalifa, Sara M</creatorcontrib><creatorcontrib>Abd El-Aziz, Abeer M</creatorcontrib><creatorcontrib>Hassan, Ramadan</creatorcontrib><creatorcontrib>Abdelmegeed, Eman S</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing and Allied Health Journals</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest_Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>https://resources.nclive.org/materials</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest advanced technologies & aerospace journals</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials science collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khalifa, Sara M</au><au>Abd El-Aziz, Abeer M</au><au>Hassan, Ramadan</au><au>Abdelmegeed, Eman S</au><au>Karunasagar, Iddya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>β-lactam resistance associated with β-lactamase production and porin alteration in clinical isolates of E. coli and K. pneumoniae</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2021-05-20</date><risdate>2021</risdate><volume>16</volume><issue>5</issue><spage>e0251594</spage><epage>e0251594</epage><pages>e0251594-e0251594</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>β-lactam resistance represents a worldwide problem and a serious challenge for antimicrobial treatment. Hence this research was conducted to recognize several mechanisms mediating β-lactam resistance in E. coli and K. pneumoniae clinical isolates collected from Mansoura University hospitals, Egypt. A total of 80 isolates, 45 E. coli and 35 K. pneumoniae isolates, were collected and their antibiotic susceptibility was determined by the Disc diffusion method followed by phenotypic and genotypic detection of extended-spectrum β-lactamases (ESBLs), AmpC β-lactamase, carbapenemase enzymes. The outer membrane protein porins of all isolates were analyzed and their genes were examined using gene amplification and sequencing. Also, the resistance to complement-mediated serum killing was estimated. A significant percentage of isolates (93.8%) were multidrug resistance and showed an elevated resistance to β-lactam antibiotics. The presence of either ESBL or AmpC enzymes was high among isolates (83.75%). Also, 60% of the isolated strains were carbapenemase producers. The most frequently detected gene of ESBL among all tested isolates was blaCTX-M-15 (86.3%) followed by blaTEM-1 (81.3%) and blaSHV-1 (35%) while the Amp-C gene was present in 83.75%. For carbapenemase-producing isolates, blaNDM1 was the most common (60%) followed by blaVIM-1 (35%) and blaOXA-48 (13.8%). Besides, 73.3% and 40% of E. coli and K. pneumoniae isolates respectively were serum resistant. Outer membrane protein analysis showed that 93.3% of E. coli and 95.7% of K. pneumoniae isolates lost their porins or showed modified porins. Furthermore, sequence analysis of tested porin genes in some isolates revealed the presence of frameshift mutations that produced truncated proteins of smaller size. β-lactam resistance in K. pneumoniae and E. coli isolates in our hospitals is due to a combination of β-lactamase activity and porin loss/alteration. Hence more restrictions should be applied on β-lactams usage to decrease the emergence of resistant strains.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>34014957</pmid><doi>10.1371/journal.pone.0251594</doi><orcidid>https://orcid.org/0000-0002-6214-5091</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2021-05, Vol.16 (5), p.e0251594-e0251594 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2529909165 |
| source | Public Library of Science (PLoS) Journals Open Access; Free E-Journal (出版社公開部分のみ）; DOAJ Directory of Open Access Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Amides Antibiotics Antimicrobial agents Bacilli Bacteria Biology and Life Sciences Clinical isolates E coli Efflux Enzymes Gene amplification Gram-negative bacilli Gram-negative bacteria Immunology Laboratories Medicine and Health Sciences Membrane permeability Microbiology Mutation Penicillin People and Places Permeability Pharmacy Proteins Research and Analysis Methods Strains (organisms) Therapeutic targets β Lactamase β-Lactam antibiotics |
| title | β-lactam resistance associated with β-lactamase production and porin alteration in clinical isolates of E. coli and K. pneumoniae |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T09%3A26%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=%CE%B2-lactam%20resistance%20associated%20with%20%CE%B2-lactamase%20production%20and%20porin%20alteration%20in%20clinical%20isolates%20of%20E.%20coli%20and%20K.%20pneumoniae&rft.jtitle=PloS%20one&rft.au=Khalifa,%20Sara%20M&rft.date=2021-05-20&rft.volume=16&rft.issue=5&rft.spage=e0251594&rft.epage=e0251594&rft.pages=e0251594-e0251594&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0251594&rft_dat=%3Cproquest_plos_%3E2529909165%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2529909165&rft_id=info:pmid/34014957&rft_doaj_id=oai_doaj_org_article_4627f6ef30c245478378253de53e845e&rfr_iscdi=true |