UV light-emitting diode (UV-LED) at 265 nm as a potential light source for disinfecting human platelet concentrates
The risk of sepsis through bacterial transmission is one of the most serious problems in platelet transfusion. In processing platelet concentrates (PCs), several methods have been put into practice to minimize the risk of bacterial transmission, such as stringent monitoring by cultivation assays and...
Gespeichert in:
| Veröffentlicht in: | PloS one 2021-05, Vol.16 (5), p.e0251650-e0251650 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0251650 |
|---|---|
| container_issue | 5 |
| container_start_page | e0251650 |
| container_title | PloS one |
| container_volume | 16 |
| creator | Hayashi, Tomoya Oguma, Kumiko Fujimura, Yoshihiro Furuta, Rika A Tanaka, Mitsunobu Masaki, Mikako Shinbata, Yasuhito Kimura, Takafumi Tani, Yoshihiko Hirayama, Fumiya Takihara, Yoshihiro Takahashi, Koki |
| description | The risk of sepsis through bacterial transmission is one of the most serious problems in platelet transfusion. In processing platelet concentrates (PCs), several methods have been put into practice to minimize the risk of bacterial transmission, such as stringent monitoring by cultivation assays and inactivation treatment by photoirradiation with or without chemical agents. As another potential option, we applied a light-emitting diode (LED) with a peak emission wavelength of 265 nm, which has been shown to be effective for water, to disinfect PCs. In a bench-scale UV-LED exposure setup, a 10-min irradiation, corresponding to an average fluence of 9.2 mJ/cm2, resulted in >2.0 log, 1.0 log, and 0.6 log inactivation (mean, n = 6) of Escherichia coli, Staphylococcus aureus, and Bacillus cereus, respectively, in non-diluted plasma PCs. After a 30-min exposure, platelet counts decreased slightly (18 ± 7%: mean ± SD, n = 7); however, platelet surface expressions of CD42b, CD61, CD62P, and PAC-1 binding did not change significantly (P>0.005), and agonist-induced aggregation and adhesion/aggregation under flow conditions were well maintained. Our findings indicated that the 265 nm UV-LED has high potential as a novel disinfection method to ensure the microbial safety of platelet transfusion. |
| doi_str_mv | 10.1371/journal.pone.0251650 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2529909137</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A662418817</galeid><doaj_id>oai_doaj_org_article_eba895aea34f4e7c97672990c03a0dc9</doaj_id><sourcerecordid>A662418817</sourcerecordid><originalsourceid>FETCH-LOGICAL-c659t-7a32166caf7adec3a82b3eab1612a60607f30dbfae462c0048acb81fb7202063</originalsourceid><addsrcrecordid>eNptUk1v1DAUjBCIlsI_QGCJSzlk8UfiJBekqhSotBKXtlfrxXnJeuXES-wg8e9xmrTqosoHf82M3zxPkrxndMNEwb7s3TQOYDcHN-CG8pzJnL5ITlkleCo5FS-frE-SN97vKc1FKeXr5ERklGVVUZ4m_vaOWNPtQoq9CcEMHWmMa5Cc396l26tvnwkEwmVOhp6AJ0AOLuAQDNiFRnwsQyNp3RiJ3gwt6nuV3dTDQA4WAloMRLtBR94Yt_5t8qoF6_HdOp8lN9-vbi5_pttfP64vL7aplnkV0gIEZ1JqaAtoUAsoeS0QaiYZB0klLVpBm7oFzCTXlGYl6LpkbV1wyqkUZ8nHRfZgnVdru7ziOa8qWsUeRsT1gmgc7NVhND2Mf5UDo-4P3NgpGIPRFhXWUFY5IIiszbDQVSGLWUdTAbTRVdT6ur421T02i1l7JHp8M5id6twfVTIhyzyLAuerwOh-T-iD6o3XaC0M6Ka5bsE4z0Q1O_v0H_R5dyuqg2gg_oyL7-pZVF1IyTNWlmxGbZ5BxdHEQMRfw9bE8yNCthD06LwfsX30yKiak_lQjJqTqdZkRtqHp_15JD1EUfwDC5rgHA</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2529909137</pqid></control><display><type>article</type><title>UV light-emitting diode (UV-LED) at 265 nm as a potential light source for disinfecting human platelet concentrates</title><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Hayashi, Tomoya ; Oguma, Kumiko ; Fujimura, Yoshihiro ; Furuta, Rika A ; Tanaka, Mitsunobu ; Masaki, Mikako ; Shinbata, Yasuhito ; Kimura, Takafumi ; Tani, Yoshihiko ; Hirayama, Fumiya ; Takihara, Yoshihiro ; Takahashi, Koki</creator><contributor>Hamblin, Michael R.</contributor><creatorcontrib>Hayashi, Tomoya ; Oguma, Kumiko ; Fujimura, Yoshihiro ; Furuta, Rika A ; Tanaka, Mitsunobu ; Masaki, Mikako ; Shinbata, Yasuhito ; Kimura, Takafumi ; Tani, Yoshihiko ; Hirayama, Fumiya ; Takihara, Yoshihiro ; Takahashi, Koki ; Hamblin, Michael R.</creatorcontrib><description>The risk of sepsis through bacterial transmission is one of the most serious problems in platelet transfusion. In processing platelet concentrates (PCs), several methods have been put into practice to minimize the risk of bacterial transmission, such as stringent monitoring by cultivation assays and inactivation treatment by photoirradiation with or without chemical agents. As another potential option, we applied a light-emitting diode (LED) with a peak emission wavelength of 265 nm, which has been shown to be effective for water, to disinfect PCs. In a bench-scale UV-LED exposure setup, a 10-min irradiation, corresponding to an average fluence of 9.2 mJ/cm2, resulted in >2.0 log, 1.0 log, and 0.6 log inactivation (mean, n = 6) of Escherichia coli, Staphylococcus aureus, and Bacillus cereus, respectively, in non-diluted plasma PCs. After a 30-min exposure, platelet counts decreased slightly (18 ± 7%: mean ± SD, n = 7); however, platelet surface expressions of CD42b, CD61, CD62P, and PAC-1 binding did not change significantly (P>0.005), and agonist-induced aggregation and adhesion/aggregation under flow conditions were well maintained. Our findings indicated that the 265 nm UV-LED has high potential as a novel disinfection method to ensure the microbial safety of platelet transfusion.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0251650</identifier><identifier>PMID: 34014978</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Auroral kilometric radiation ; Bacteria ; Biology and Life Sciences ; Blood ; Blood platelets ; Cloning ; Cold ; Cold storage ; Culture ; Deactivation ; Disinfection ; E coli ; Editing ; Flow velocity ; Glycoproteins ; Health aspects ; High flow ; Inactivation ; Infection control ; Light emitting diodes ; Light sources ; Medicine and Health Sciences ; Methods ; Pathogens ; Platelets ; Radiation ; Regulatory agencies ; Reviews ; Room temperature ; Shelf life ; Sterilization ; Transfusion ; Ultraviolet radiation ; Water treatment</subject><ispartof>PloS one, 2021-05, Vol.16 (5), p.e0251650-e0251650</ispartof><rights>COPYRIGHT 2021 Public Library of Science</rights><rights>2021 Hayashi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 Hayashi et al 2021 Hayashi et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c659t-7a32166caf7adec3a82b3eab1612a60607f30dbfae462c0048acb81fb7202063</citedby><cites>FETCH-LOGICAL-c659t-7a32166caf7adec3a82b3eab1612a60607f30dbfae462c0048acb81fb7202063</cites><orcidid>0000-0002-1776-0052</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8136854/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8136854/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2101,2927,23865,27923,27924,53790,53792,79471,79472</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34014978$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Hamblin, Michael R.</contributor><creatorcontrib>Hayashi, Tomoya</creatorcontrib><creatorcontrib>Oguma, Kumiko</creatorcontrib><creatorcontrib>Fujimura, Yoshihiro</creatorcontrib><creatorcontrib>Furuta, Rika A</creatorcontrib><creatorcontrib>Tanaka, Mitsunobu</creatorcontrib><creatorcontrib>Masaki, Mikako</creatorcontrib><creatorcontrib>Shinbata, Yasuhito</creatorcontrib><creatorcontrib>Kimura, Takafumi</creatorcontrib><creatorcontrib>Tani, Yoshihiko</creatorcontrib><creatorcontrib>Hirayama, Fumiya</creatorcontrib><creatorcontrib>Takihara, Yoshihiro</creatorcontrib><creatorcontrib>Takahashi, Koki</creatorcontrib><title>UV light-emitting diode (UV-LED) at 265 nm as a potential light source for disinfecting human platelet concentrates</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The risk of sepsis through bacterial transmission is one of the most serious problems in platelet transfusion. In processing platelet concentrates (PCs), several methods have been put into practice to minimize the risk of bacterial transmission, such as stringent monitoring by cultivation assays and inactivation treatment by photoirradiation with or without chemical agents. As another potential option, we applied a light-emitting diode (LED) with a peak emission wavelength of 265 nm, which has been shown to be effective for water, to disinfect PCs. In a bench-scale UV-LED exposure setup, a 10-min irradiation, corresponding to an average fluence of 9.2 mJ/cm2, resulted in >2.0 log, 1.0 log, and 0.6 log inactivation (mean, n = 6) of Escherichia coli, Staphylococcus aureus, and Bacillus cereus, respectively, in non-diluted plasma PCs. After a 30-min exposure, platelet counts decreased slightly (18 ± 7%: mean ± SD, n = 7); however, platelet surface expressions of CD42b, CD61, CD62P, and PAC-1 binding did not change significantly (P>0.005), and agonist-induced aggregation and adhesion/aggregation under flow conditions were well maintained. Our findings indicated that the 265 nm UV-LED has high potential as a novel disinfection method to ensure the microbial safety of platelet transfusion.</description><subject>Auroral kilometric radiation</subject><subject>Bacteria</subject><subject>Biology and Life Sciences</subject><subject>Blood</subject><subject>Blood platelets</subject><subject>Cloning</subject><subject>Cold</subject><subject>Cold storage</subject><subject>Culture</subject><subject>Deactivation</subject><subject>Disinfection</subject><subject>E coli</subject><subject>Editing</subject><subject>Flow velocity</subject><subject>Glycoproteins</subject><subject>Health aspects</subject><subject>High flow</subject><subject>Inactivation</subject><subject>Infection control</subject><subject>Light emitting diodes</subject><subject>Light sources</subject><subject>Medicine and Health Sciences</subject><subject>Methods</subject><subject>Pathogens</subject><subject>Platelets</subject><subject>Radiation</subject><subject>Regulatory agencies</subject><subject>Reviews</subject><subject>Room temperature</subject><subject>Shelf life</subject><subject>Sterilization</subject><subject>Transfusion</subject><subject>Ultraviolet radiation</subject><subject>Water treatment</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptUk1v1DAUjBCIlsI_QGCJSzlk8UfiJBekqhSotBKXtlfrxXnJeuXES-wg8e9xmrTqosoHf82M3zxPkrxndMNEwb7s3TQOYDcHN-CG8pzJnL5ITlkleCo5FS-frE-SN97vKc1FKeXr5ERklGVVUZ4m_vaOWNPtQoq9CcEMHWmMa5Cc396l26tvnwkEwmVOhp6AJ0AOLuAQDNiFRnwsQyNp3RiJ3gwt6nuV3dTDQA4WAloMRLtBR94Yt_5t8qoF6_HdOp8lN9-vbi5_pttfP64vL7aplnkV0gIEZ1JqaAtoUAsoeS0QaiYZB0klLVpBm7oFzCTXlGYl6LpkbV1wyqkUZ8nHRfZgnVdru7ziOa8qWsUeRsT1gmgc7NVhND2Mf5UDo-4P3NgpGIPRFhXWUFY5IIiszbDQVSGLWUdTAbTRVdT6ur421T02i1l7JHp8M5id6twfVTIhyzyLAuerwOh-T-iD6o3XaC0M6Ka5bsE4z0Q1O_v0H_R5dyuqg2gg_oyL7-pZVF1IyTNWlmxGbZ5BxdHEQMRfw9bE8yNCthD06LwfsX30yKiak_lQjJqTqdZkRtqHp_15JD1EUfwDC5rgHA</recordid><startdate>20210520</startdate><enddate>20210520</enddate><creator>Hayashi, Tomoya</creator><creator>Oguma, Kumiko</creator><creator>Fujimura, Yoshihiro</creator><creator>Furuta, Rika A</creator><creator>Tanaka, Mitsunobu</creator><creator>Masaki, Mikako</creator><creator>Shinbata, Yasuhito</creator><creator>Kimura, Takafumi</creator><creator>Tani, Yoshihiko</creator><creator>Hirayama, Fumiya</creator><creator>Takihara, Yoshihiro</creator><creator>Takahashi, Koki</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-1776-0052</orcidid></search><sort><creationdate>20210520</creationdate><title>UV light-emitting diode (UV-LED) at 265 nm as a potential light source for disinfecting human platelet concentrates</title><author>Hayashi, Tomoya ; Oguma, Kumiko ; Fujimura, Yoshihiro ; Furuta, Rika A ; Tanaka, Mitsunobu ; Masaki, Mikako ; Shinbata, Yasuhito ; Kimura, Takafumi ; Tani, Yoshihiko ; Hirayama, Fumiya ; Takihara, Yoshihiro ; Takahashi, Koki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c659t-7a32166caf7adec3a82b3eab1612a60607f30dbfae462c0048acb81fb7202063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Auroral kilometric radiation</topic><topic>Bacteria</topic><topic>Biology and Life Sciences</topic><topic>Blood</topic><topic>Blood platelets</topic><topic>Cloning</topic><topic>Cold</topic><topic>Cold storage</topic><topic>Culture</topic><topic>Deactivation</topic><topic>Disinfection</topic><topic>E coli</topic><topic>Editing</topic><topic>Flow velocity</topic><topic>Glycoproteins</topic><topic>Health aspects</topic><topic>High flow</topic><topic>Inactivation</topic><topic>Infection control</topic><topic>Light emitting diodes</topic><topic>Light sources</topic><topic>Medicine and Health Sciences</topic><topic>Methods</topic><topic>Pathogens</topic><topic>Platelets</topic><topic>Radiation</topic><topic>Regulatory agencies</topic><topic>Reviews</topic><topic>Room temperature</topic><topic>Shelf life</topic><topic>Sterilization</topic><topic>Transfusion</topic><topic>Ultraviolet radiation</topic><topic>Water treatment</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hayashi, Tomoya</creatorcontrib><creatorcontrib>Oguma, Kumiko</creatorcontrib><creatorcontrib>Fujimura, Yoshihiro</creatorcontrib><creatorcontrib>Furuta, Rika A</creatorcontrib><creatorcontrib>Tanaka, Mitsunobu</creatorcontrib><creatorcontrib>Masaki, Mikako</creatorcontrib><creatorcontrib>Shinbata, Yasuhito</creatorcontrib><creatorcontrib>Kimura, Takafumi</creatorcontrib><creatorcontrib>Tani, Yoshihiko</creatorcontrib><creatorcontrib>Hirayama, Fumiya</creatorcontrib><creatorcontrib>Takihara, Yoshihiro</creatorcontrib><creatorcontrib>Takahashi, Koki</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hayashi, Tomoya</au><au>Oguma, Kumiko</au><au>Fujimura, Yoshihiro</au><au>Furuta, Rika A</au><au>Tanaka, Mitsunobu</au><au>Masaki, Mikako</au><au>Shinbata, Yasuhito</au><au>Kimura, Takafumi</au><au>Tani, Yoshihiko</au><au>Hirayama, Fumiya</au><au>Takihara, Yoshihiro</au><au>Takahashi, Koki</au><au>Hamblin, Michael R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>UV light-emitting diode (UV-LED) at 265 nm as a potential light source for disinfecting human platelet concentrates</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2021-05-20</date><risdate>2021</risdate><volume>16</volume><issue>5</issue><spage>e0251650</spage><epage>e0251650</epage><pages>e0251650-e0251650</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The risk of sepsis through bacterial transmission is one of the most serious problems in platelet transfusion. In processing platelet concentrates (PCs), several methods have been put into practice to minimize the risk of bacterial transmission, such as stringent monitoring by cultivation assays and inactivation treatment by photoirradiation with or without chemical agents. As another potential option, we applied a light-emitting diode (LED) with a peak emission wavelength of 265 nm, which has been shown to be effective for water, to disinfect PCs. In a bench-scale UV-LED exposure setup, a 10-min irradiation, corresponding to an average fluence of 9.2 mJ/cm2, resulted in >2.0 log, 1.0 log, and 0.6 log inactivation (mean, n = 6) of Escherichia coli, Staphylococcus aureus, and Bacillus cereus, respectively, in non-diluted plasma PCs. After a 30-min exposure, platelet counts decreased slightly (18 ± 7%: mean ± SD, n = 7); however, platelet surface expressions of CD42b, CD61, CD62P, and PAC-1 binding did not change significantly (P>0.005), and agonist-induced aggregation and adhesion/aggregation under flow conditions were well maintained. Our findings indicated that the 265 nm UV-LED has high potential as a novel disinfection method to ensure the microbial safety of platelet transfusion.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>34014978</pmid><doi>10.1371/journal.pone.0251650</doi><orcidid>https://orcid.org/0000-0002-1776-0052</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2021-05, Vol.16 (5), p.e0251650-e0251650 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2529909137 |
| source | DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Auroral kilometric radiation Bacteria Biology and Life Sciences Blood Blood platelets Cloning Cold Cold storage Culture Deactivation Disinfection E coli Editing Flow velocity Glycoproteins Health aspects High flow Inactivation Infection control Light emitting diodes Light sources Medicine and Health Sciences Methods Pathogens Platelets Radiation Regulatory agencies Reviews Room temperature Shelf life Sterilization Transfusion Ultraviolet radiation Water treatment |
| title | UV light-emitting diode (UV-LED) at 265 nm as a potential light source for disinfecting human platelet concentrates |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T06%3A17%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=UV%20light-emitting%20diode%20(UV-LED)%20at%20265%20nm%20as%20a%20potential%20light%20source%20for%20disinfecting%20human%20platelet%20concentrates&rft.jtitle=PloS%20one&rft.au=Hayashi,%20Tomoya&rft.date=2021-05-20&rft.volume=16&rft.issue=5&rft.spage=e0251650&rft.epage=e0251650&rft.pages=e0251650-e0251650&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0251650&rft_dat=%3Cgale_plos_%3EA662418817%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2529909137&rft_id=info:pmid/34014978&rft_galeid=A662418817&rft_doaj_id=oai_doaj_org_article_eba895aea34f4e7c97672990c03a0dc9&rfr_iscdi=true |