A robust method of nuclei isolation for single-cell RNA sequencing of solid tissues from the plant genus Populus

Single-cell transcriptome analysis has been extensively applied in humans and animal models to uncover gene expression heterogeneity between the different cell types of a tissue or an organ. It demonstrated its capability to discover key regulatory elements that determine cell fate during developmen...

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Veröffentlicht in:	PloS one 2021-05, Vol.16 (5), p.e0251149-e0251149
Hauptverfasser:	Conde, Daniel, Triozzi, Paolo M, Balmant, Kelly M, Doty, Andria L, Miranda, Mariza, Boullosa, Anthony, Schmidt, Henry W, Pereira, Wendell J, Dervinis, Christopher, Kirst, Matias
Format:	Artikel
Sprache:	eng
Schlagworte:	Aluminum Analysis Animal models BASIC BIOLOGICAL SCIENCES Biology and Life Sciences Biotechnology Cell culture Cell Fractionation - methods Dissociation Editing Engineering and Technology ENVIRONMENTAL SCIENCES External stimuli Flow cytometry Flowers & plants Gene expression Gene Expression Profiling - methods Gene sequencing Genetic aspects Genetics Genomic analysis Glass plates Imaging Interdisciplinary aspects Medical instruments Methodology Methods Organs Plant tissues Poplar Populus - genetics Protoplasts Reproducibility of Results Research and analysis methods Reviews Ribonuclease RNA sequencing RNA, Plant Science & Technology - Other Topics Sequence Analysis, RNA Single-Cell Analysis Sucrose Transcription Transcriptomes Tubes
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