Development of a loop-mediated isothermal amplification assay combined with a nanoparticle-based lateral flow biosensor for rapid detection of plasmid-mediated colistin resistance gene mcr-1

A loop-mediated isothermal amplification assay combined with a nanoparticle-based lateral flow biosensor (LAMP-LFB) was established for the rapid and accurate detection of the mobilized colistin resistance gene (mcr-1), which causes the loss of colistin antibacterial efficacy in clinical treatments....

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Veröffentlicht in:	PloS one 2021-04, Vol.16 (4), p.e0249582
Hauptverfasser:	Gong, Lin, Tang, Fei, Liu, Ernan, Liu, Xiaoli, Xu, Huiqiong, Wang, Yimei, Song, Yadong, Liang, Jiansheng
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibacterial agents Antibiotics Antiinfectives and antibacterials Bacteria Biochemical assays Biology and Life Sciences Biosensors Biotin Colistin Deoxyribonucleic acid Disease control Disease prevention Disinfection DNA DNA polymerase DNA synthesis Drug resistance Drug resistance in microorganisms E coli Empirical analysis Engineering and Technology Gene amplification Genes Genetic aspects Infections Medicine and Health Sciences Methodology Methods Multidrug resistance Nanoparticles Pest control Pest resistance Pests Prevention Primers Research and Analysis Methods Room temperature Salmonella
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