Versatile and flexible microfluidic qPCR test for high-throughput SARS-CoV-2 and cellular response detection in nasopharyngeal swab samples

The emergence and quick spread of SARS-CoV-2 has pointed at a low capacity response for testing large populations in many countries, in line of material, technical and staff limitations. The traditional RT-qPCR diagnostic test remains the reference method and is by far the most widely used test. The...

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Veröffentlicht in:	PloS one 2021-04, Vol.16 (4), p.e0243333-e0243333
Hauptverfasser:	Fassy, Julien, Lacoux, Caroline, Leroy, Sylvie, Noussair, Latifa, Hubac, Sylvain, Degoutte, Aurélien, Vassaux, Georges, Leclercq, Vianney, Rouquié, David, Marquette, Charles-Hugo, Rottman, Martin, Touron, Patrick, Lemoine, Antoinette, Herrmann, Jean-Louis, Barbry, Pascal, Nahon, Jean-Louis, Zaragosi, Laure-Emmanuelle, Mari, Bernard
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Automation Bioengineering Biology and life sciences Coronaviruses COVID-19 COVID-19 - diagnosis COVID-19 - virology COVID-19 Testing - methods Deoxyribonucleic acid Diagnostic Tests, Routine - methods DNA DNA Primers Editing Enzyme-linked immunosorbent assay Female Funding Genes Humans Infections Laboratories Life Sciences Male Medicine and health sciences Methodology Methods Microfluidic Analytical Techniques - methods Microfluidics MicroRNAs - genetics Molecular diagnostic techniques Nucleocapsids Nucleotide sequence Pandemics Pharmacology Physical Sciences Polymerase chain reaction Reagents Real-Time Polymerase Chain Reaction - methods Research and analysis methods Reviews RNA, Viral - genetics SARS-CoV-2 - genetics SARS-CoV-2 - isolation & purification Sensitivity and Specificity Severe acute respiratory syndrome Severe acute respiratory syndrome coronavirus 2 Shortages Specimen Handling - methods Thermal cycling Viral diseases Viruses
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