Establishment of a well-characterized SARS-CoV-2 lentiviral pseudovirus neutralization assay using 293T cells with stable expression of ACE2 and TMPRSS2

Pseudoviruses are useful surrogates for highly pathogenic viruses because of their safety, genetic stability, and scalability for screening assays. Many different pseudovirus platforms exist, each with different advantages and limitations. Here we report our efforts to optimize and characterize an H...

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Veröffentlicht in:	PloS one 2021-03, Vol.16 (3), p.e0248348-e0248348
Hauptverfasser:	Neerukonda, Sabari Nath, Vassell, Russell, Herrup, Rachel, Liu, Shufeng, Wang, Tony, Takeda, Kazuyo, Yang, Ye, Lin, Tsai-Lien, Wang, Wei, Weiss, Carol D
Format:	Artikel
Sprache:	eng
Schlagworte:	ACE inhibitors ACE2 Acquired immune deficiency syndrome AIDS Angiotensin-converting enzyme 2 Angiotensin-Converting Enzyme 2 - genetics Angiotensin-Converting Enzyme 2 - immunology Antibodies, Neutralizing - immunology Antibodies, Viral - immunology Biology and life sciences Coronaviruses COVID-19 COVID-19 - metabolism Diagnosis Drug Evaluation, Preclinical - methods Editing Enzymatic activity Epidemics Epidemiology Evaluation Fireflies Fluorescence Gag protein Genetic aspects Glycoproteins HEK293 Cells HIV Human immunodeficiency virus Humans Lentivirus - metabolism Leukemia Medicine and health sciences Methodology Neon Neutralization Neutralization Tests - methods Plasmids Prevention Proteases Regulatory agencies Research and analysis methods Research facilities SARS-CoV-2 - metabolism SARS-CoV-2 - pathogenicity Severe acute respiratory syndrome Severe acute respiratory syndrome coronavirus 2 Spike Glycoprotein, Coronavirus - genetics Stomatitis Testing United States Vaccines Viruses
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