Fine mapping epitope on Glycoprotein-Gn from Severe Fever with Thrombocytopenia Syndrome Virus
Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) was recently identified as a tick-borne pathogen that threat to human health. Since 2010, many countries including China, South Korea, and Japan have reported Human SFTS caused by SFTSV infection. The glycoprotein encoded by the SFTSV M gene...
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| description | Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) was recently identified as a tick-borne pathogen that threat to human health. Since 2010, many countries including China, South Korea, and Japan have reported Human SFTS caused by SFTSV infection. The glycoprotein encoded by the SFTSV M gene is the major antigenic component on the viral surface, and responsible for the viral entry, which makes it an important viral antigen and a clinical diagnostic target. The present study aimed to map linear B cell epitopes (BCEs) on the N-terminal glycoprotein (Gn) from SFTSV strain WCH/97/HN/China/2011 using the modified biosynthetic peptide method. Five fine epitopes (E1, 196FSQSEFPD203; E2, 232GHSHKII238; E3, 256VCYKEGTGPC265; E4, 285FCKVAG290, and E5, 316SYGGM320) were identified using the rabbit antisera. Western blot analysis showed that all the five epitopes interacted with the positive serum of sheep that had been naturally infected with SFTSV. Three-dimensional structural modeling analysis showed that all identified BCEs were located on the surface of the SFTSV-Gn and contained flexible loops. The sequence alignment revealed high conservation of the identified BCEs among 13 SFTSV strains from different lineage. These mapped epitopes will escalate the understanding of the epitope distribution and pathogenic mechanism of SFTSV, and could provide a basis for the development of a SFTSV multi-epitope detection antigen. |
| doi_str_mv | 10.1371/journal.pone.0248005 |
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Since 2010, many countries including China, South Korea, and Japan have reported Human SFTS caused by SFTSV infection. The glycoprotein encoded by the SFTSV M gene is the major antigenic component on the viral surface, and responsible for the viral entry, which makes it an important viral antigen and a clinical diagnostic target. The present study aimed to map linear B cell epitopes (BCEs) on the N-terminal glycoprotein (Gn) from SFTSV strain WCH/97/HN/China/2011 using the modified biosynthetic peptide method. Five fine epitopes (E1, 196FSQSEFPD203; E2, 232GHSHKII238; E3, 256VCYKEGTGPC265; E4, 285FCKVAG290, and E5, 316SYGGM320) were identified using the rabbit antisera. Western blot analysis showed that all the five epitopes interacted with the positive serum of sheep that had been naturally infected with SFTSV. Three-dimensional structural modeling analysis showed that all identified BCEs were located on the surface of the SFTSV-Gn and contained flexible loops. The sequence alignment revealed high conservation of the identified BCEs among 13 SFTSV strains from different lineage. These mapped epitopes will escalate the understanding of the epitope distribution and pathogenic mechanism of SFTSV, and could provide a basis for the development of a SFTSV multi-epitope detection antigen.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0248005</identifier><identifier>PMID: 33651850</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Antibodies ; Biology and Life Sciences ; Bites ; Cloning ; Computer and Information Sciences ; Computer programs ; Diagnosis ; Disease control ; Disease prevention ; Drafting software ; Electronic mail ; Engineering and Technology ; Epitope mapping ; Ethics ; Exocytosis ; Fever ; Funding ; Gene mapping ; Genetic aspects ; Genetic engineering ; Glycoproteins ; Health aspects ; Laboratories ; Life sciences ; Livestock ; Mail ; Medicine and Health Sciences ; Methodology ; Methods ; Mortality ; Mucus ; Peptides ; Plasmids ; Prevention ; Proteins ; Research and Analysis Methods ; Risk factors ; RNA polymerase ; Science and technology ; Sheep ; Software ; Technology ; Thrombocytopenia ; Vaccine development ; Vaccines ; Viral infections ; Virions ; Virology ; Viruses</subject><ispartof>PloS one, 2021-03, Vol.16 (3), p.e0248005-e0248005</ispartof><rights>COPYRIGHT 2021 Public Library of Science</rights><rights>2021 Moming et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 Moming et al 2021 Moming et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-bafb26607e1eeb2b449eec5c84cd81018b7cf29d5e3e32a0e2dc09cdc34d90983</citedby><cites>FETCH-LOGICAL-c692t-bafb26607e1eeb2b449eec5c84cd81018b7cf29d5e3e32a0e2dc09cdc34d90983</cites><orcidid>0000-0003-3633-1904</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924767/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924767/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53769,53771,79346,79347</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33651850$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Ho, Paulo Lee</contributor><creatorcontrib>Moming, Abulimiti</creatorcontrib><creatorcontrib>Shi, Shen</creatorcontrib><creatorcontrib>Shen, Shu</creatorcontrib><creatorcontrib>Qiao, Jie</creatorcontrib><creatorcontrib>Yue, Xihong</creatorcontrib><creatorcontrib>Wang, Bo</creatorcontrib><creatorcontrib>Ding, Juntao</creatorcontrib><creatorcontrib>Hu, Zhihong</creatorcontrib><creatorcontrib>Deng, Fei</creatorcontrib><creatorcontrib>Zhang, Yujiang</creatorcontrib><creatorcontrib>Sun, Surong</creatorcontrib><title>Fine mapping epitope on Glycoprotein-Gn from Severe Fever with Thrombocytopenia Syndrome Virus</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) was recently identified as a tick-borne pathogen that threat to human health. 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The sequence alignment revealed high conservation of the identified BCEs among 13 SFTSV strains from different lineage. These mapped epitopes will escalate the understanding of the epitope distribution and pathogenic mechanism of SFTSV, and could provide a basis for the development of a SFTSV multi-epitope detection antigen.</description><subject>Analysis</subject><subject>Antibodies</subject><subject>Biology and Life Sciences</subject><subject>Bites</subject><subject>Cloning</subject><subject>Computer and Information Sciences</subject><subject>Computer programs</subject><subject>Diagnosis</subject><subject>Disease control</subject><subject>Disease prevention</subject><subject>Drafting software</subject><subject>Electronic mail</subject><subject>Engineering and Technology</subject><subject>Epitope mapping</subject><subject>Ethics</subject><subject>Exocytosis</subject><subject>Fever</subject><subject>Funding</subject><subject>Gene mapping</subject><subject>Genetic aspects</subject><subject>Genetic engineering</subject><subject>Glycoproteins</subject><subject>Health 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tick-borne pathogen that threat to human health. Since 2010, many countries including China, South Korea, and Japan have reported Human SFTS caused by SFTSV infection. The glycoprotein encoded by the SFTSV M gene is the major antigenic component on the viral surface, and responsible for the viral entry, which makes it an important viral antigen and a clinical diagnostic target. The present study aimed to map linear B cell epitopes (BCEs) on the N-terminal glycoprotein (Gn) from SFTSV strain WCH/97/HN/China/2011 using the modified biosynthetic peptide method. Five fine epitopes (E1, 196FSQSEFPD203; E2, 232GHSHKII238; E3, 256VCYKEGTGPC265; E4, 285FCKVAG290, and E5, 316SYGGM320) were identified using the rabbit antisera. Western blot analysis showed that all the five epitopes interacted with the positive serum of sheep that had been naturally infected with SFTSV. Three-dimensional structural modeling analysis showed that all identified BCEs were located on the surface of the SFTSV-Gn and contained flexible loops. The sequence alignment revealed high conservation of the identified BCEs among 13 SFTSV strains from different lineage. These mapped epitopes will escalate the understanding of the epitope distribution and pathogenic mechanism of SFTSV, and could provide a basis for the development of a SFTSV multi-epitope detection antigen.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>33651850</pmid><doi>10.1371/journal.pone.0248005</doi><orcidid>https://orcid.org/0000-0003-3633-1904</orcidid><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis Antibodies Biology and Life Sciences Bites Cloning Computer and Information Sciences Computer programs Diagnosis Disease control Disease prevention Drafting software Electronic mail Engineering and Technology Epitope mapping Ethics Exocytosis Fever Funding Gene mapping Genetic aspects Genetic engineering Glycoproteins Health aspects Laboratories Life sciences Livestock Medicine and Health Sciences Methodology Methods Mortality Mucus Peptides Plasmids Prevention Proteins Research and Analysis Methods Risk factors RNA polymerase Science and technology Sheep Software Technology Thrombocytopenia Vaccine development Vaccines Viral infections Virions Virology Viruses |
| title | Fine mapping epitope on Glycoprotein-Gn from Severe Fever with Thrombocytopenia Syndrome Virus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-26T18%3A22%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Fine%20mapping%20epitope%20on%20Glycoprotein-Gn%20from%20Severe%20Fever%20with%20Thrombocytopenia%20Syndrome%20Virus&rft.jtitle=PloS%20one&rft.au=Moming,%20Abulimiti&rft.date=2021-03-02&rft.volume=16&rft.issue=3&rft.spage=e0248005&rft.epage=e0248005&rft.pages=e0248005-e0248005&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0248005&rft_dat=%3Cgale_plos_%3EA653565854%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2495373064&rft_id=info:pmid/33651850&rft_galeid=A653565854&rft_doaj_id=oai_doaj_org_article_9aa74dff1f724953bbe94b300df0ca99&rfr_iscdi=true |