An optimized workflow for CRISPR-Cas9 deletion of surface and intracellular factors in primary human T lymphocytes

The appropriate regulation of T lymphocyte functions is key to achieve protective immune responses, while at the same time limiting the risks of tissue damage and chronic inflammation. Deciphering the mechanisms underpinning T cell responses in humans may therefore be beneficial for a range of infec...

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Veröffentlicht in:	PloS one 2021-02, Vol.16 (2), p.e0247232-e0247232
Hauptverfasser:	Leoni, Cristina, Bianchi, Niccolò, Vincenzetti, Lucia, Monticelli, Silvia
Format:	Artikel
Sprache:	eng
Schlagworte:	Ablation Amino acids Antibodies Availability Biology and Life Sciences Blood & organ donations Cell culture Cell Cycle Proteins - deficiency Cell Cycle Proteins - genetics Cells, Cultured Cloning CRISPR CRISPR-Cas Systems - genetics Deletion Deoxyribonucleic acid DNA Drafting software Efficiency Endoribonucleases - deficiency Endoribonucleases - genetics Gene Editing - methods Genetic aspects Genetic modification Genomes Genomics Glutamine Graduate schools Graduate studies Health aspects Humans Immune response Insertion Interrogation Lymphocytes Lymphocytes T Medicine and Health Sciences Membrane Proteins - deficiency Membrane Proteins - genetics Methodology miRNA Non-coding RNA Penicillin Proteins Pyruvic acid Questioning Receptors, Antigen, T-Cell, alpha-beta - deficiency Receptors, Antigen, T-Cell, alpha-beta - genetics Research and Analysis Methods RNA, Guide, CRISPR-Cas Systems - genetics RNA, Guide, CRISPR-Cas Systems - metabolism Sodium pyruvate Streptomycin Subpopulations T cells T-Box Domain Proteins - deficiency T-Box Domain Proteins - genetics T-Lymphocytes - cytology T-Lymphocytes - metabolism Transcription Factors - deficiency Transcription Factors - genetics Western blotting Workflow
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