Enzymatically polymerised polyphenols prepared from various precursors potentiate antigen-specific immune responses in both mucosal and systemic compartments in mice
Despite significant modern medicine progress, having an infectious disease is a major risk factor for humans. Mucosal vaccination is now widely considered as the most promising strategy to defeat infectious diseases; however, only live-attenuated and inactivated mucosal vaccines are used in the clin...
Gespeichert in:
Veröffentlicht in: | PloS one 2021-02, Vol.16 (2), p.e0246422 |
---|---|
Hauptverfasser: | , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | |
---|---|
container_issue | 2 |
container_start_page | e0246422 |
container_title | PloS one |
container_volume | 16 |
creator | Tada, Rui Ogasawara, Miki Yamanaka, Daisuke Sakurai, Yasuhiro Negishi, Yoichi Kiyono, Hiroshi Ohno, Naohito Kunisawa, Jun Aramaki, Yukihiko |
description | Despite significant modern medicine progress, having an infectious disease is a major risk factor for humans. Mucosal vaccination is now widely considered as the most promising strategy to defeat infectious diseases; however, only live-attenuated and inactivated mucosal vaccines are used in the clinical field. To date, no subunit mucosal vaccine was approved mainly because of the lack of safe and effective methodologies to either activate or initiate host mucosal immune responses. We have recently elucidated that intranasal administration of enzymatically polymerised caffeic acid potentiates antigen-specific mucosal and systemic antibody responses in mice. However, our earlier study has not confirmed whether these effects are specific to the polymer synthesised from caffeic acid. Here, we show that enzymatically polymerised polyphenols (EPPs) from various phenolic compounds possess mucosal adjuvant activities when administered nasally with an antigen to mice. Potentiation of antigen-specific immune responses by all EPPs tested in this study showed no clear difference among the precursors used. We found that intranasal administration of ovalbumin as the antigen, in combination with all enzymatically polymerised polyphenols used in this study, induced ovalbumin-specific mucosal IgA in the nasal cavity, bronchoalveolar lavage fluid, vaginal fluids, and systemic IgG, especially IgG1, in sera. Our results demonstrate that the mucosal adjuvant activities of polyphenols are not limited to polymerised caffeic acid but are broadly observable across the studied polyphenols. These properties of polyphenols may be advantageous for the development of safe and effective nasal vaccine systems to prevent and/or treat various infectious diseases. |
doi_str_mv | 10.1371/journal.pone.0246422 |
format | Article |
fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2487425098</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A651160916</galeid><doaj_id>oai_doaj_org_article_91751b6c8c18418f905c6b80a4661c7d</doaj_id><sourcerecordid>A651160916</sourcerecordid><originalsourceid>FETCH-LOGICAL-c758t-2e3aaf77e6c8b2ccbae8ec7ad5ed0fd2bfe1aa484e779c4936b3ec3cb7cbc5b43</originalsourceid><addsrcrecordid>eNqNk9-K1DAUxoso7rr6BqIFQfBixqRpk_ZmYVlWHVhY8N9tSE9PZ7KkTU3SxfF9fE8zM91lCgqSixy-_s6X05OcJHlJyZIyQd_f2tH1yiwH2-OSZDnPs-xRckorli14Rtjjo_gkeeb9LSEFKzl_mpwwVhSc0uo0-X3V_9p2KmhQxmzTwZpth057bPbxsMHeGp8ODgflotg626V3ymk77lUYnbcuhjZgH7QKmKq4r7Ff-AFBtxpS3XVjj6lDH2v16FPdp7UNm7QbwXplYkaT-q0P2EUabBePCl2025NRw-fJk1YZjy-m_Sz59uHq6-WnxfXNx9XlxfUCRFGGRYZMqVYI5FDWGUCtsEQQqimwIW2T1S1SpfIyRyEqyCvGa4bAoBZQQ1Hn7Cx5ffAdjPVy6rCXWV6KPCtIVUZidSAaq27l4HSn3FZapeVesG4tY_EaDMqKioLWsRSgZU7LtiIF8LokKuecgmii1_l02lh32ED8Y6fMzHT-pdcbubZ3UpSCEJJFgzeTgbM_RvThHyVP1FrFqnTf2mgGnfYgL3hBKScV5ZFa_oWKq9ndSnxjrY76LOHdLCEyAX-GtRq9l6svn_-fvfk-Z98esRtUJmy8NWPQ8fHMwfwAgrPeO2wfOkeJ3I3IfTfkbkTkNCIx7dVx1x-S7meC_QFILhNh</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2487425098</pqid></control><display><type>article</type><title>Enzymatically polymerised polyphenols prepared from various precursors potentiate antigen-specific immune responses in both mucosal and systemic compartments in mice</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Public Library of Science (PLoS)</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Tada, Rui ; Ogasawara, Miki ; Yamanaka, Daisuke ; Sakurai, Yasuhiro ; Negishi, Yoichi ; Kiyono, Hiroshi ; Ohno, Naohito ; Kunisawa, Jun ; Aramaki, Yukihiko</creator><contributor>Murthy, Ashlesh K.</contributor><creatorcontrib>Tada, Rui ; Ogasawara, Miki ; Yamanaka, Daisuke ; Sakurai, Yasuhiro ; Negishi, Yoichi ; Kiyono, Hiroshi ; Ohno, Naohito ; Kunisawa, Jun ; Aramaki, Yukihiko ; Murthy, Ashlesh K.</creatorcontrib><description>Despite significant modern medicine progress, having an infectious disease is a major risk factor for humans. Mucosal vaccination is now widely considered as the most promising strategy to defeat infectious diseases; however, only live-attenuated and inactivated mucosal vaccines are used in the clinical field. To date, no subunit mucosal vaccine was approved mainly because of the lack of safe and effective methodologies to either activate or initiate host mucosal immune responses. We have recently elucidated that intranasal administration of enzymatically polymerised caffeic acid potentiates antigen-specific mucosal and systemic antibody responses in mice. However, our earlier study has not confirmed whether these effects are specific to the polymer synthesised from caffeic acid. Here, we show that enzymatically polymerised polyphenols (EPPs) from various phenolic compounds possess mucosal adjuvant activities when administered nasally with an antigen to mice. Potentiation of antigen-specific immune responses by all EPPs tested in this study showed no clear difference among the precursors used. We found that intranasal administration of ovalbumin as the antigen, in combination with all enzymatically polymerised polyphenols used in this study, induced ovalbumin-specific mucosal IgA in the nasal cavity, bronchoalveolar lavage fluid, vaginal fluids, and systemic IgG, especially IgG1, in sera. Our results demonstrate that the mucosal adjuvant activities of polyphenols are not limited to polymerised caffeic acid but are broadly observable across the studied polyphenols. These properties of polyphenols may be advantageous for the development of safe and effective nasal vaccine systems to prevent and/or treat various infectious diseases.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0246422</identifier><identifier>PMID: 33556119</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acids ; Adjuvants ; Antigenicity ; Antigens ; Biology and Life Sciences ; Chemical properties ; Communicable diseases ; Cytotoxicity ; Enzymes ; Experiments ; Health aspects ; Immune response ; Immunology ; Infectious diseases ; Laboratory animals ; Lamina propria ; Life sciences ; Medicine and Health Sciences ; Microorganisms ; Mucosal immunity ; Pathogens ; Pharmacy ; Polyphenols ; Precursors ; Prevention ; R&D ; Research & development ; Research and Analysis Methods ; Streptococcus infections ; Testing ; Toxicity ; Vaccines</subject><ispartof>PloS one, 2021-02, Vol.16 (2), p.e0246422</ispartof><rights>COPYRIGHT 2021 Public Library of Science</rights><rights>2021 Tada et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 Tada et al 2021 Tada et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-2e3aaf77e6c8b2ccbae8ec7ad5ed0fd2bfe1aa484e779c4936b3ec3cb7cbc5b43</citedby><cites>FETCH-LOGICAL-c758t-2e3aaf77e6c8b2ccbae8ec7ad5ed0fd2bfe1aa484e779c4936b3ec3cb7cbc5b43</cites><orcidid>0000-0003-0098-9587</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870002/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870002/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,862,883,2098,2917,23853,27911,27912,53778,53780,79355,79356</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33556119$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Murthy, Ashlesh K.</contributor><creatorcontrib>Tada, Rui</creatorcontrib><creatorcontrib>Ogasawara, Miki</creatorcontrib><creatorcontrib>Yamanaka, Daisuke</creatorcontrib><creatorcontrib>Sakurai, Yasuhiro</creatorcontrib><creatorcontrib>Negishi, Yoichi</creatorcontrib><creatorcontrib>Kiyono, Hiroshi</creatorcontrib><creatorcontrib>Ohno, Naohito</creatorcontrib><creatorcontrib>Kunisawa, Jun</creatorcontrib><creatorcontrib>Aramaki, Yukihiko</creatorcontrib><title>Enzymatically polymerised polyphenols prepared from various precursors potentiate antigen-specific immune responses in both mucosal and systemic compartments in mice</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Despite significant modern medicine progress, having an infectious disease is a major risk factor for humans. Mucosal vaccination is now widely considered as the most promising strategy to defeat infectious diseases; however, only live-attenuated and inactivated mucosal vaccines are used in the clinical field. To date, no subunit mucosal vaccine was approved mainly because of the lack of safe and effective methodologies to either activate or initiate host mucosal immune responses. We have recently elucidated that intranasal administration of enzymatically polymerised caffeic acid potentiates antigen-specific mucosal and systemic antibody responses in mice. However, our earlier study has not confirmed whether these effects are specific to the polymer synthesised from caffeic acid. Here, we show that enzymatically polymerised polyphenols (EPPs) from various phenolic compounds possess mucosal adjuvant activities when administered nasally with an antigen to mice. Potentiation of antigen-specific immune responses by all EPPs tested in this study showed no clear difference among the precursors used. We found that intranasal administration of ovalbumin as the antigen, in combination with all enzymatically polymerised polyphenols used in this study, induced ovalbumin-specific mucosal IgA in the nasal cavity, bronchoalveolar lavage fluid, vaginal fluids, and systemic IgG, especially IgG1, in sera. Our results demonstrate that the mucosal adjuvant activities of polyphenols are not limited to polymerised caffeic acid but are broadly observable across the studied polyphenols. These properties of polyphenols may be advantageous for the development of safe and effective nasal vaccine systems to prevent and/or treat various infectious diseases.</description><subject>Acids</subject><subject>Adjuvants</subject><subject>Antigenicity</subject><subject>Antigens</subject><subject>Biology and Life Sciences</subject><subject>Chemical properties</subject><subject>Communicable diseases</subject><subject>Cytotoxicity</subject><subject>Enzymes</subject><subject>Experiments</subject><subject>Health aspects</subject><subject>Immune response</subject><subject>Immunology</subject><subject>Infectious diseases</subject><subject>Laboratory animals</subject><subject>Lamina propria</subject><subject>Life sciences</subject><subject>Medicine and Health Sciences</subject><subject>Microorganisms</subject><subject>Mucosal immunity</subject><subject>Pathogens</subject><subject>Pharmacy</subject><subject>Polyphenols</subject><subject>Precursors</subject><subject>Prevention</subject><subject>R&D</subject><subject>Research & development</subject><subject>Research and Analysis Methods</subject><subject>Streptococcus infections</subject><subject>Testing</subject><subject>Toxicity</subject><subject>Vaccines</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9-K1DAUxoso7rr6BqIFQfBixqRpk_ZmYVlWHVhY8N9tSE9PZ7KkTU3SxfF9fE8zM91lCgqSixy-_s6X05OcJHlJyZIyQd_f2tH1yiwH2-OSZDnPs-xRckorli14Rtjjo_gkeeb9LSEFKzl_mpwwVhSc0uo0-X3V_9p2KmhQxmzTwZpth057bPbxsMHeGp8ODgflotg626V3ymk77lUYnbcuhjZgH7QKmKq4r7Ff-AFBtxpS3XVjj6lDH2v16FPdp7UNm7QbwXplYkaT-q0P2EUabBePCl2025NRw-fJk1YZjy-m_Sz59uHq6-WnxfXNx9XlxfUCRFGGRYZMqVYI5FDWGUCtsEQQqimwIW2T1S1SpfIyRyEqyCvGa4bAoBZQQ1Hn7Cx5ffAdjPVy6rCXWV6KPCtIVUZidSAaq27l4HSn3FZapeVesG4tY_EaDMqKioLWsRSgZU7LtiIF8LokKuecgmii1_l02lh32ED8Y6fMzHT-pdcbubZ3UpSCEJJFgzeTgbM_RvThHyVP1FrFqnTf2mgGnfYgL3hBKScV5ZFa_oWKq9ndSnxjrY76LOHdLCEyAX-GtRq9l6svn_-fvfk-Z98esRtUJmy8NWPQ8fHMwfwAgrPeO2wfOkeJ3I3IfTfkbkTkNCIx7dVx1x-S7meC_QFILhNh</recordid><startdate>20210208</startdate><enddate>20210208</enddate><creator>Tada, Rui</creator><creator>Ogasawara, Miki</creator><creator>Yamanaka, Daisuke</creator><creator>Sakurai, Yasuhiro</creator><creator>Negishi, Yoichi</creator><creator>Kiyono, Hiroshi</creator><creator>Ohno, Naohito</creator><creator>Kunisawa, Jun</creator><creator>Aramaki, Yukihiko</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-0098-9587</orcidid></search><sort><creationdate>20210208</creationdate><title>Enzymatically polymerised polyphenols prepared from various precursors potentiate antigen-specific immune responses in both mucosal and systemic compartments in mice</title><author>Tada, Rui ; Ogasawara, Miki ; Yamanaka, Daisuke ; Sakurai, Yasuhiro ; Negishi, Yoichi ; Kiyono, Hiroshi ; Ohno, Naohito ; Kunisawa, Jun ; Aramaki, Yukihiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-2e3aaf77e6c8b2ccbae8ec7ad5ed0fd2bfe1aa484e779c4936b3ec3cb7cbc5b43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Acids</topic><topic>Adjuvants</topic><topic>Antigenicity</topic><topic>Antigens</topic><topic>Biology and Life Sciences</topic><topic>Chemical properties</topic><topic>Communicable diseases</topic><topic>Cytotoxicity</topic><topic>Enzymes</topic><topic>Experiments</topic><topic>Health aspects</topic><topic>Immune response</topic><topic>Immunology</topic><topic>Infectious diseases</topic><topic>Laboratory animals</topic><topic>Lamina propria</topic><topic>Life sciences</topic><topic>Medicine and Health Sciences</topic><topic>Microorganisms</topic><topic>Mucosal immunity</topic><topic>Pathogens</topic><topic>Pharmacy</topic><topic>Polyphenols</topic><topic>Precursors</topic><topic>Prevention</topic><topic>R&D</topic><topic>Research & development</topic><topic>Research and Analysis Methods</topic><topic>Streptococcus infections</topic><topic>Testing</topic><topic>Toxicity</topic><topic>Vaccines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tada, Rui</creatorcontrib><creatorcontrib>Ogasawara, Miki</creatorcontrib><creatorcontrib>Yamanaka, Daisuke</creatorcontrib><creatorcontrib>Sakurai, Yasuhiro</creatorcontrib><creatorcontrib>Negishi, Yoichi</creatorcontrib><creatorcontrib>Kiyono, Hiroshi</creatorcontrib><creatorcontrib>Ohno, Naohito</creatorcontrib><creatorcontrib>Kunisawa, Jun</creatorcontrib><creatorcontrib>Aramaki, Yukihiko</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tada, Rui</au><au>Ogasawara, Miki</au><au>Yamanaka, Daisuke</au><au>Sakurai, Yasuhiro</au><au>Negishi, Yoichi</au><au>Kiyono, Hiroshi</au><au>Ohno, Naohito</au><au>Kunisawa, Jun</au><au>Aramaki, Yukihiko</au><au>Murthy, Ashlesh K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzymatically polymerised polyphenols prepared from various precursors potentiate antigen-specific immune responses in both mucosal and systemic compartments in mice</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2021-02-08</date><risdate>2021</risdate><volume>16</volume><issue>2</issue><spage>e0246422</spage><pages>e0246422-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Despite significant modern medicine progress, having an infectious disease is a major risk factor for humans. Mucosal vaccination is now widely considered as the most promising strategy to defeat infectious diseases; however, only live-attenuated and inactivated mucosal vaccines are used in the clinical field. To date, no subunit mucosal vaccine was approved mainly because of the lack of safe and effective methodologies to either activate or initiate host mucosal immune responses. We have recently elucidated that intranasal administration of enzymatically polymerised caffeic acid potentiates antigen-specific mucosal and systemic antibody responses in mice. However, our earlier study has not confirmed whether these effects are specific to the polymer synthesised from caffeic acid. Here, we show that enzymatically polymerised polyphenols (EPPs) from various phenolic compounds possess mucosal adjuvant activities when administered nasally with an antigen to mice. Potentiation of antigen-specific immune responses by all EPPs tested in this study showed no clear difference among the precursors used. We found that intranasal administration of ovalbumin as the antigen, in combination with all enzymatically polymerised polyphenols used in this study, induced ovalbumin-specific mucosal IgA in the nasal cavity, bronchoalveolar lavage fluid, vaginal fluids, and systemic IgG, especially IgG1, in sera. Our results demonstrate that the mucosal adjuvant activities of polyphenols are not limited to polymerised caffeic acid but are broadly observable across the studied polyphenols. These properties of polyphenols may be advantageous for the development of safe and effective nasal vaccine systems to prevent and/or treat various infectious diseases.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>33556119</pmid><doi>10.1371/journal.pone.0246422</doi><tpages>e0246422</tpages><orcidid>https://orcid.org/0000-0003-0098-9587</orcidid><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1932-6203 |
ispartof | PloS one, 2021-02, Vol.16 (2), p.e0246422 |
issn | 1932-6203 1932-6203 |
language | eng |
recordid | cdi_plos_journals_2487425098 |
source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
subjects | Acids Adjuvants Antigenicity Antigens Biology and Life Sciences Chemical properties Communicable diseases Cytotoxicity Enzymes Experiments Health aspects Immune response Immunology Infectious diseases Laboratory animals Lamina propria Life sciences Medicine and Health Sciences Microorganisms Mucosal immunity Pathogens Pharmacy Polyphenols Precursors Prevention R&D Research & development Research and Analysis Methods Streptococcus infections Testing Toxicity Vaccines |
title | Enzymatically polymerised polyphenols prepared from various precursors potentiate antigen-specific immune responses in both mucosal and systemic compartments in mice |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T20%3A21%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Enzymatically%20polymerised%20polyphenols%20prepared%20from%20various%20precursors%20potentiate%20antigen-specific%20immune%20responses%20in%20both%20mucosal%20and%20systemic%20compartments%20in%20mice&rft.jtitle=PloS%20one&rft.au=Tada,%20Rui&rft.date=2021-02-08&rft.volume=16&rft.issue=2&rft.spage=e0246422&rft.pages=e0246422-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0246422&rft_dat=%3Cgale_plos_%3EA651160916%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2487425098&rft_id=info:pmid/33556119&rft_galeid=A651160916&rft_doaj_id=oai_doaj_org_article_91751b6c8c18418f905c6b80a4661c7d&rfr_iscdi=true |