Extracellular and intracellular intermittent magnetic-fluid hyperthermia treatment of SK-Hep1 hepatocellular carcinoma cells based on magnetic nanoparticles coated with polystyrene sulfonic acid
The use of magnetic nanoparticles (MNPs) magnetized on applying an alternating magnetic field (AMF) to stimulate the thermal characteristics and to induce tumor apoptosis is a currently active area of research in cancer treatment. In previous work, we developed biocompatible and superparamagnetic po...
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| creator | Chen, Bo-Wei Chiu, Guo-Wei He, Yun-Chi Huang, Chih-Yu Huang, Hao-Ting Sung, Shian-Ying Hsieh, Chia-Ling Chang, Wei-Chieh Hsu, Ming-Shinn Wei, Zung-Hang Yao, Da-Jeng |
| description | The use of magnetic nanoparticles (MNPs) magnetized on applying an alternating magnetic field (AMF) to stimulate the thermal characteristics and to induce tumor apoptosis is a currently active area of research in cancer treatment. In previous work, we developed biocompatible and superparamagnetic polystyrene-sulfonic-acid-coated magnetic nanoparticles (PSS-MNPs) as applications for magnetically labeled cell trapping, but without assessment of treatment effects on tumor diseases. In the present work, we examined PSS-MNP-induced magnetic fluid hyperthermia (MFH) on SK-Hep1 hepatocellular carcinoma (HCC) cells for lethal thermal effects with a self-made AMF system; an adjustable AMF frequency generated a variable intensity of magnetic field and induced MNP relaxation. The extracellular and intracellular MFH treatments on a SK-Hep1 cell line were implemented in vitro; the result indicates that the lethal effects were efficient and caused a significantly decreased cell viability of SK-Hep1 cells. As the PSS-MNP concentration decreased, especially in intracellular MFH treatments, the MFH effects on cells, however, largely decreased through heat spreading to the culture medium. On controlling and decreasing the volume of culture medium, the problem of heat spreading was solved. It can be consequently expected that PSS-MNPs would be a prospective agent for intracellular cancer magnetotherapy. |
| doi_str_mv | 10.1371/journal.pone.0245286 |
| format | Article |
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In previous work, we developed biocompatible and superparamagnetic polystyrene-sulfonic-acid-coated magnetic nanoparticles (PSS-MNPs) as applications for magnetically labeled cell trapping, but without assessment of treatment effects on tumor diseases. In the present work, we examined PSS-MNP-induced magnetic fluid hyperthermia (MFH) on SK-Hep1 hepatocellular carcinoma (HCC) cells for lethal thermal effects with a self-made AMF system; an adjustable AMF frequency generated a variable intensity of magnetic field and induced MNP relaxation. The extracellular and intracellular MFH treatments on a SK-Hep1 cell line were implemented in vitro; the result indicates that the lethal effects were efficient and caused a significantly decreased cell viability of SK-Hep1 cells. As the PSS-MNP concentration decreased, especially in intracellular MFH treatments, the MFH effects on cells, however, largely decreased through heat spreading to the culture medium. On controlling and decreasing the volume of culture medium, the problem of heat spreading was solved. It can be consequently expected that PSS-MNPs would be a prospective agent for intracellular cancer magnetotherapy.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0245286</identifier><identifier>PMID: 33544751</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Apoptosis ; Biology and Life Sciences ; Biomaterials ; Biomedical materials ; Cancer ; Cancer therapies ; Care and treatment ; Cell culture ; Cervical cancer ; Cervix ; Chemotherapy ; Dimethyl sulfoxide ; Efficiency ; Engineering and Technology ; Fetal calf serum ; Fever ; Glutamine ; Glutaraldehyde ; Gynecology ; Health aspects ; Heat ; Hepatocellular carcinoma ; Hepatoma ; Hyperthermia ; Iodides ; Iron oxides ; Lethal effects ; Liver cancer ; Magnetic fields ; Magnetic properties ; Materials ; Mechanical engineering ; Medical research ; Medical science ; Medicine ; Medicine and Health Sciences ; Nanoengineering ; Nanoparticles ; Neurosurgery ; Obstetrics ; Penicillin ; Physical Sciences ; Polystyrene ; Polystyrene resins ; Potassium ; Propidium iodide ; Radiation therapy ; Reagents ; Research and Analysis Methods ; Research facilities ; Science and technology ; Sodium chloride ; Streptomycin ; Sulfonic acid ; Temperature rise ; Thermal properties ; Translation ; Trypsin</subject><ispartof>PloS one, 2021-02, Vol.16 (2), p.e0245286-e0245286</ispartof><rights>COPYRIGHT 2021 Public Library of Science</rights><rights>2021 Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 Chen et al 2021 Chen et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-e561a7926974a13b8ccbc5110fed7073a105ec0175e555c863356a95ba1898813</citedby><cites>FETCH-LOGICAL-c692t-e561a7926974a13b8ccbc5110fed7073a105ec0175e555c863356a95ba1898813</cites><orcidid>0000-0003-4243-4707 ; 0000-0001-6631-780X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7864458/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7864458/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79569,79570</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33544751$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Bo-Wei</creatorcontrib><creatorcontrib>Chiu, Guo-Wei</creatorcontrib><creatorcontrib>He, Yun-Chi</creatorcontrib><creatorcontrib>Huang, Chih-Yu</creatorcontrib><creatorcontrib>Huang, Hao-Ting</creatorcontrib><creatorcontrib>Sung, Shian-Ying</creatorcontrib><creatorcontrib>Hsieh, Chia-Ling</creatorcontrib><creatorcontrib>Chang, Wei-Chieh</creatorcontrib><creatorcontrib>Hsu, Ming-Shinn</creatorcontrib><creatorcontrib>Wei, Zung-Hang</creatorcontrib><creatorcontrib>Yao, Da-Jeng</creatorcontrib><title>Extracellular and intracellular intermittent magnetic-fluid hyperthermia treatment of SK-Hep1 hepatocellular carcinoma cells based on magnetic nanoparticles coated with polystyrene sulfonic acid</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The use of magnetic nanoparticles (MNPs) magnetized on applying an alternating magnetic field (AMF) to stimulate the thermal characteristics and to induce tumor apoptosis is a currently active area of research in cancer treatment. In previous work, we developed biocompatible and superparamagnetic polystyrene-sulfonic-acid-coated magnetic nanoparticles (PSS-MNPs) as applications for magnetically labeled cell trapping, but without assessment of treatment effects on tumor diseases. In the present work, we examined PSS-MNP-induced magnetic fluid hyperthermia (MFH) on SK-Hep1 hepatocellular carcinoma (HCC) cells for lethal thermal effects with a self-made AMF system; an adjustable AMF frequency generated a variable intensity of magnetic field and induced MNP relaxation. The extracellular and intracellular MFH treatments on a SK-Hep1 cell line were implemented in vitro; the result indicates that the lethal effects were efficient and caused a significantly decreased cell viability of SK-Hep1 cells. As the PSS-MNP concentration decreased, especially in intracellular MFH treatments, the MFH effects on cells, however, largely decreased through heat spreading to the culture medium. On controlling and decreasing the volume of culture medium, the problem of heat spreading was solved. It can be consequently expected that PSS-MNPs would be a prospective agent for intracellular cancer magnetotherapy.</description><subject>Apoptosis</subject><subject>Biology and Life Sciences</subject><subject>Biomaterials</subject><subject>Biomedical materials</subject><subject>Cancer</subject><subject>Cancer therapies</subject><subject>Care and treatment</subject><subject>Cell culture</subject><subject>Cervical cancer</subject><subject>Cervix</subject><subject>Chemotherapy</subject><subject>Dimethyl sulfoxide</subject><subject>Efficiency</subject><subject>Engineering and Technology</subject><subject>Fetal calf serum</subject><subject>Fever</subject><subject>Glutamine</subject><subject>Glutaraldehyde</subject><subject>Gynecology</subject><subject>Health aspects</subject><subject>Heat</subject><subject>Hepatocellular carcinoma</subject><subject>Hepatoma</subject><subject>Hyperthermia</subject><subject>Iodides</subject><subject>Iron oxides</subject><subject>Lethal 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SK-Hep1 hepatocellular carcinoma cells based on magnetic nanoparticles coated with polystyrene sulfonic acid</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2021-02-05</date><risdate>2021</risdate><volume>16</volume><issue>2</issue><spage>e0245286</spage><epage>e0245286</epage><pages>e0245286-e0245286</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The use of magnetic nanoparticles (MNPs) magnetized on applying an alternating magnetic field (AMF) to stimulate the thermal characteristics and to induce tumor apoptosis is a currently active area of research in cancer treatment. In previous work, we developed biocompatible and superparamagnetic polystyrene-sulfonic-acid-coated magnetic nanoparticles (PSS-MNPs) as applications for magnetically labeled cell trapping, but without assessment of treatment effects on tumor diseases. In the present work, we examined PSS-MNP-induced magnetic fluid hyperthermia (MFH) on SK-Hep1 hepatocellular carcinoma (HCC) cells for lethal thermal effects with a self-made AMF system; an adjustable AMF frequency generated a variable intensity of magnetic field and induced MNP relaxation. The extracellular and intracellular MFH treatments on a SK-Hep1 cell line were implemented in vitro; the result indicates that the lethal effects were efficient and caused a significantly decreased cell viability of SK-Hep1 cells. As the PSS-MNP concentration decreased, especially in intracellular MFH treatments, the MFH effects on cells, however, largely decreased through heat spreading to the culture medium. On controlling and decreasing the volume of culture medium, the problem of heat spreading was solved. It can be consequently expected that PSS-MNPs would be a prospective agent for intracellular cancer magnetotherapy.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>33544751</pmid><doi>10.1371/journal.pone.0245286</doi><tpages>e0245286</tpages><orcidid>https://orcid.org/0000-0003-4243-4707</orcidid><orcidid>https://orcid.org/0000-0001-6631-780X</orcidid><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_2486844562 |
| source | Public Library of Science (PLoS) Journals Open Access; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Apoptosis Biology and Life Sciences Biomaterials Biomedical materials Cancer Cancer therapies Care and treatment Cell culture Cervical cancer Cervix Chemotherapy Dimethyl sulfoxide Efficiency Engineering and Technology Fetal calf serum Fever Glutamine Glutaraldehyde Gynecology Health aspects Heat Hepatocellular carcinoma Hepatoma Hyperthermia Iodides Iron oxides Lethal effects Liver cancer Magnetic fields Magnetic properties Materials Mechanical engineering Medical research Medical science Medicine Medicine and Health Sciences Nanoengineering Nanoparticles Neurosurgery Obstetrics Penicillin Physical Sciences Polystyrene Polystyrene resins Potassium Propidium iodide Radiation therapy Reagents Research and Analysis Methods Research facilities Science and technology Sodium chloride Streptomycin Sulfonic acid Temperature rise Thermal properties Translation Trypsin |
| title | Extracellular and intracellular intermittent magnetic-fluid hyperthermia treatment of SK-Hep1 hepatocellular carcinoma cells based on magnetic nanoparticles coated with polystyrene sulfonic acid |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-13T17%3A06%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Extracellular%20and%20intracellular%20intermittent%20magnetic-fluid%20hyperthermia%20treatment%20of%20SK-Hep1%20hepatocellular%20carcinoma%20cells%20based%20on%20magnetic%20nanoparticles%20coated%20with%20polystyrene%20sulfonic%20acid&rft.jtitle=PloS%20one&rft.au=Chen,%20Bo-Wei&rft.date=2021-02-05&rft.volume=16&rft.issue=2&rft.spage=e0245286&rft.epage=e0245286&rft.pages=e0245286-e0245286&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0245286&rft_dat=%3Cgale_plos_%3EA650856547%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2486844562&rft_id=info:pmid/33544751&rft_galeid=A650856547&rft_doaj_id=oai_doaj_org_article_bb7dd58e910c4fecac5c8d2a5b5f3afb&rfr_iscdi=true |