Effects of Chinese wolfberry and Astragalus extract on the antioxidant capacity of Tibetan pig liver
The objective of this study is to determine the effect of Chinese wolfberry (Lycium barbarum) and Astragalus (Astragalus membranaceus) extract (WAE) on the antioxidant capacity of Tibetan pig liver, and discussed the regulatory effect of WAE on the liver antioxidant mechanism. Twelve healthy 120-day...
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| description | The objective of this study is to determine the effect of Chinese wolfberry (Lycium barbarum) and Astragalus (Astragalus membranaceus) extract (WAE) on the antioxidant capacity of Tibetan pig liver, and discussed the regulatory effect of WAE on the liver antioxidant mechanism. Twelve healthy 120-day-old Tibetan black pigs (35±2 kg) were divided randomly into two groups. The WAE group was fed a basal diet supplemented with 1% WAE for 90 days. The control group was fed the same diet, but without the WAE. We found that liver superoxide dismutase 1 (SOD1) activity (P |
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Twelve healthy 120-day-old Tibetan black pigs (35±2 kg) were divided randomly into two groups. The WAE group was fed a basal diet supplemented with 1% WAE for 90 days. The control group was fed the same diet, but without the WAE. We found that liver superoxide dismutase 1 (SOD1) activity (P<0.05), total antioxidative capacity (T-AOC) (P<0.05), and catalase (CAT) activity (P<0.01) significantly increased in the WAE group compared with the control group; malondialdehyde (MDA) content decreased, but this was not significant (P >0.05). Transcriptome sequencing analysis detected 106 differentially expressed genes (DEGs) related to oxidative stress. GO enrichment analysis showed these DEGs were involved in the positive regulation of reactive oxygen metabolism and biosynthesis, process regulation, and regulation of the oxidative stress response. KEGG Pathway enrichment analysis showed they were enriched in the PI3K-Akt, AMPK, Rap1, and peroxisome signaling pathways. The expression levels of key peroxisome biosynthesis genes (e.g., PEX3 and PEX11B) and key antioxidant genes (e.g., CAT and SOD1) were significantly higher in the WAE group than in the control group. The PRDX1 and PRDX5 content also was significantly higher in the WAE group. This study showed that the WAE regulated the antioxidant and anti-stress ability of Tibetan pig liver through a "peroxisome antioxidant-oxidant stress" signaling pathway.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0245749</identifier><identifier>PMID: 33503027</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animal husbandry ; Animal sciences ; Animals ; Antioxidants ; Antioxidants - pharmacology ; Astragalus (Plants) ; Astragalus membranaceus ; Astragalus Plant - chemistry ; Biology and Life Sciences ; Biotechnology industry ; Breeding ; Breeding methods ; Chemical synthesis ; Coliforms ; Deoxyribonucleic acid ; Diet ; DNA ; DNA biosynthesis ; DNA polymerase ; DNA-directed DNA polymerase ; E coli ; Economic impact ; Enzymes ; Exonuclease ; Free radicals ; Gases ; Gene expression ; Genomes ; Health aspects ; High fat diet ; High protein diet ; High temperature ; Hogs ; Immune response ; Ingredients ; Intestine ; Laboratory animals ; Liver ; Liver - drug effects ; Liver - metabolism ; Lycium ; Lycium - chemistry ; Lycium barbarum ; Oligonucleotides ; Oxidative stress ; People and Places ; Peroxins - genetics ; Peroxins - metabolism ; Peroxisomes - metabolism ; Physiological aspects ; Plant extracts ; Plant Extracts - pharmacology ; Polysaccharides ; Ribonuclease H ; Saccharides ; Signal Transduction ; Swine ; Weaning ; Zoology</subject><ispartof>PloS one, 2021-01, Vol.16 (1), p.e0245749-e0245749</ispartof><rights>COPYRIGHT 2021 Public Library of Science</rights><rights>2021 Hao et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 Hao et al 2021 Hao et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-7e50e1176fc1289e6ac169a01410db43ce5073ea412abc5dbed2b1b5c57a9fb73</citedby><cites>FETCH-LOGICAL-c692t-7e50e1176fc1289e6ac169a01410db43ce5073ea412abc5dbed2b1b5c57a9fb73</cites><orcidid>0000-0001-7713-8604</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840052/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840052/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,2926,23865,27923,27924,53790,53792,79371,79372</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33503027$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Aceto, Serena</contributor><creatorcontrib>Hao, Zhuang</creatorcontrib><creatorcontrib>Li, Zhen</creatorcontrib><creatorcontrib>Huo, Jinjin</creatorcontrib><creatorcontrib>Li, Jiandong</creatorcontrib><creatorcontrib>Liu, Fenghua</creatorcontrib><creatorcontrib>Yin, Peng</creatorcontrib><title>Effects of Chinese wolfberry and Astragalus extract on the antioxidant capacity of Tibetan pig liver</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The objective of this study is to determine the effect of Chinese wolfberry (Lycium barbarum) and Astragalus (Astragalus membranaceus) extract (WAE) on the antioxidant capacity of Tibetan pig liver, and discussed the regulatory effect of WAE on the liver antioxidant mechanism. Twelve healthy 120-day-old Tibetan black pigs (35±2 kg) were divided randomly into two groups. The WAE group was fed a basal diet supplemented with 1% WAE for 90 days. The control group was fed the same diet, but without the WAE. We found that liver superoxide dismutase 1 (SOD1) activity (P<0.05), total antioxidative capacity (T-AOC) (P<0.05), and catalase (CAT) activity (P<0.01) significantly increased in the WAE group compared with the control group; malondialdehyde (MDA) content decreased, but this was not significant (P >0.05). Transcriptome sequencing analysis detected 106 differentially expressed genes (DEGs) related to oxidative stress. GO enrichment analysis showed these DEGs were involved in the positive regulation of reactive oxygen metabolism and biosynthesis, process regulation, and regulation of the oxidative stress response. KEGG Pathway enrichment analysis showed they were enriched in the PI3K-Akt, AMPK, Rap1, and peroxisome signaling pathways. The expression levels of key peroxisome biosynthesis genes (e.g., PEX3 and PEX11B) and key antioxidant genes (e.g., CAT and SOD1) were significantly higher in the WAE group than in the control group. The PRDX1 and PRDX5 content also was significantly higher in the WAE group. This study showed that the WAE regulated the antioxidant and anti-stress ability of Tibetan pig liver through a "peroxisome antioxidant-oxidant stress" signaling pathway.</description><subject>Animal husbandry</subject><subject>Animal sciences</subject><subject>Animals</subject><subject>Antioxidants</subject><subject>Antioxidants - pharmacology</subject><subject>Astragalus (Plants)</subject><subject>Astragalus membranaceus</subject><subject>Astragalus Plant - chemistry</subject><subject>Biology and Life Sciences</subject><subject>Biotechnology industry</subject><subject>Breeding</subject><subject>Breeding methods</subject><subject>Chemical synthesis</subject><subject>Coliforms</subject><subject>Deoxyribonucleic acid</subject><subject>Diet</subject><subject>DNA</subject><subject>DNA biosynthesis</subject><subject>DNA polymerase</subject><subject>DNA-directed DNA polymerase</subject><subject>E coli</subject><subject>Economic impact</subject><subject>Enzymes</subject><subject>Exonuclease</subject><subject>Free radicals</subject><subject>Gases</subject><subject>Gene expression</subject><subject>Genomes</subject><subject>Health aspects</subject><subject>High fat diet</subject><subject>High protein diet</subject><subject>High temperature</subject><subject>Hogs</subject><subject>Immune response</subject><subject>Ingredients</subject><subject>Intestine</subject><subject>Laboratory animals</subject><subject>Liver</subject><subject>Liver - drug effects</subject><subject>Liver - metabolism</subject><subject>Lycium</subject><subject>Lycium - chemistry</subject><subject>Lycium barbarum</subject><subject>Oligonucleotides</subject><subject>Oxidative stress</subject><subject>People and Places</subject><subject>Peroxins - genetics</subject><subject>Peroxins - metabolism</subject><subject>Peroxisomes - metabolism</subject><subject>Physiological aspects</subject><subject>Plant extracts</subject><subject>Plant Extracts - pharmacology</subject><subject>Polysaccharides</subject><subject>Ribonuclease H</subject><subject>Saccharides</subject><subject>Signal Transduction</subject><subject>Swine</subject><subject>Weaning</subject><subject>Zoology</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11v0zAUhiMEYmPwDxBEQkJw0eKvOMkNUjUNqDRpEgxuLds5aV25cbGdsf57nDWbGrQL5Atb5zzntX0-suw1RnNMS_xp43rfSTvfuQ7miLCiZPWT7BTXlMw4QfTp0fkkexHCBqGCVpw_z04oLRBFpDzNmou2BR1D7tr8fG06CJD_cbZV4P0-l12TL0L0ciVtH3K4TUcdc9flcQ3JG427NU3acy13Upu4H3SujYIou3xnVrk1N-BfZs9aaQO8Gvez7OeXi-vzb7PLq6_L88XlTPOaxFkJBQKMS95qTKoauNSY1xJhhlGjGNXJX1KQDBOpdNEoaIjCqtBFKetWlfQse3vQ3VkXxJigIAircFHWqEKJWB6IxsmN2HmzlX4vnDTizuD8SkgfjbYgSIuLpiCVUlyzquaqxBJLximAljVqktbn8bZebaHR0KXs2Ino1NOZtVi5G1FWLJWCJIEPo4B3v3sIUWxN0GCt7MD1d-8mnPOaVwl99w_6-O9GKpULhOlaN9RrEBULzuqkxvCgNX-ESquBrdGpm1qT7JOAj5OAxMTUCivZhyCWP77_P3v1a8q-P2LXIG1cB2f71FVdmILsAGrvQvDQPiQZIzEMw302xDAMYhyGFPbmuEAPQffdT_8CxDAFJQ</recordid><startdate>20210127</startdate><enddate>20210127</enddate><creator>Hao, Zhuang</creator><creator>Li, Zhen</creator><creator>Huo, Jinjin</creator><creator>Li, Jiandong</creator><creator>Liu, Fenghua</creator><creator>Yin, Peng</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-7713-8604</orcidid></search><sort><creationdate>20210127</creationdate><title>Effects of Chinese wolfberry and Astragalus extract on the antioxidant capacity of Tibetan pig liver</title><author>Hao, Zhuang ; Li, Zhen ; Huo, Jinjin ; Li, Jiandong ; Liu, Fenghua ; Yin, Peng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-7e50e1176fc1289e6ac169a01410db43ce5073ea412abc5dbed2b1b5c57a9fb73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animal husbandry</topic><topic>Animal sciences</topic><topic>Animals</topic><topic>Antioxidants</topic><topic>Antioxidants - pharmacology</topic><topic>Astragalus (Plants)</topic><topic>Astragalus membranaceus</topic><topic>Astragalus Plant - chemistry</topic><topic>Biology and Life Sciences</topic><topic>Biotechnology industry</topic><topic>Breeding</topic><topic>Breeding methods</topic><topic>Chemical synthesis</topic><topic>Coliforms</topic><topic>Deoxyribonucleic acid</topic><topic>Diet</topic><topic>DNA</topic><topic>DNA biosynthesis</topic><topic>DNA polymerase</topic><topic>DNA-directed DNA polymerase</topic><topic>E coli</topic><topic>Economic impact</topic><topic>Enzymes</topic><topic>Exonuclease</topic><topic>Free radicals</topic><topic>Gases</topic><topic>Gene expression</topic><topic>Genomes</topic><topic>Health aspects</topic><topic>High fat diet</topic><topic>High protein diet</topic><topic>High temperature</topic><topic>Hogs</topic><topic>Immune response</topic><topic>Ingredients</topic><topic>Intestine</topic><topic>Laboratory animals</topic><topic>Liver</topic><topic>Liver - drug effects</topic><topic>Liver - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hao, Zhuang</au><au>Li, Zhen</au><au>Huo, Jinjin</au><au>Li, Jiandong</au><au>Liu, Fenghua</au><au>Yin, Peng</au><au>Aceto, Serena</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Chinese wolfberry and Astragalus extract on the antioxidant capacity of Tibetan pig liver</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2021-01-27</date><risdate>2021</risdate><volume>16</volume><issue>1</issue><spage>e0245749</spage><epage>e0245749</epage><pages>e0245749-e0245749</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The objective of this study is to determine the effect of Chinese wolfberry (Lycium barbarum) and Astragalus (Astragalus membranaceus) extract (WAE) on the antioxidant capacity of Tibetan pig liver, and discussed the regulatory effect of WAE on the liver antioxidant mechanism. Twelve healthy 120-day-old Tibetan black pigs (35±2 kg) were divided randomly into two groups. The WAE group was fed a basal diet supplemented with 1% WAE for 90 days. The control group was fed the same diet, but without the WAE. We found that liver superoxide dismutase 1 (SOD1) activity (P<0.05), total antioxidative capacity (T-AOC) (P<0.05), and catalase (CAT) activity (P<0.01) significantly increased in the WAE group compared with the control group; malondialdehyde (MDA) content decreased, but this was not significant (P >0.05). Transcriptome sequencing analysis detected 106 differentially expressed genes (DEGs) related to oxidative stress. GO enrichment analysis showed these DEGs were involved in the positive regulation of reactive oxygen metabolism and biosynthesis, process regulation, and regulation of the oxidative stress response. KEGG Pathway enrichment analysis showed they were enriched in the PI3K-Akt, AMPK, Rap1, and peroxisome signaling pathways. The expression levels of key peroxisome biosynthesis genes (e.g., PEX3 and PEX11B) and key antioxidant genes (e.g., CAT and SOD1) were significantly higher in the WAE group than in the control group. The PRDX1 and PRDX5 content also was significantly higher in the WAE group. This study showed that the WAE regulated the antioxidant and anti-stress ability of Tibetan pig liver through a "peroxisome antioxidant-oxidant stress" signaling pathway.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>33503027</pmid><doi>10.1371/journal.pone.0245749</doi><tpages>e0245749</tpages><orcidid>https://orcid.org/0000-0001-7713-8604</orcidid><oa>free_for_read</oa></addata></record> |
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| issn | 1932-6203 1932-6203 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animal husbandry Animal sciences Animals Antioxidants Antioxidants - pharmacology Astragalus (Plants) Astragalus membranaceus Astragalus Plant - chemistry Biology and Life Sciences Biotechnology industry Breeding Breeding methods Chemical synthesis Coliforms Deoxyribonucleic acid Diet DNA DNA biosynthesis DNA polymerase DNA-directed DNA polymerase E coli Economic impact Enzymes Exonuclease Free radicals Gases Gene expression Genomes Health aspects High fat diet High protein diet High temperature Hogs Immune response Ingredients Intestine Laboratory animals Liver Liver - drug effects Liver - metabolism Lycium Lycium - chemistry Lycium barbarum Oligonucleotides Oxidative stress People and Places Peroxins - genetics Peroxins - metabolism Peroxisomes - metabolism Physiological aspects Plant extracts Plant Extracts - pharmacology Polysaccharides Ribonuclease H Saccharides Signal Transduction Swine Weaning Zoology |
| title | Effects of Chinese wolfberry and Astragalus extract on the antioxidant capacity of Tibetan pig liver |
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