Immunohistological detection of small particles of Echinococcus multilocularis and Echinococcus granulosus in lymph nodes is associated with enlarged lymph nodes in alveolar and cystic echinococcosis
Alveolar (AE) and cystic echinococcosis (CE) in humans are caused by the metacestode of the tapeworms Echinococcus multilocularis and Echinococcus granulosus sensu lato (s.l.). Immunohistochemistry with the monoclonal antibodies (mAb) Em2G11, specific for AE, and the mAb EmG3, specific for AE and CE...
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| Veröffentlicht in: | PLoS neglected tropical diseases 2020-12, Vol.14 (12), p.e0008921-e0008921 |
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| creator | Grimm, Johannes Nell, Juliane Hillenbrand, Andreas Henne-Bruns, Doris Schmidberger, Julian Kratzer, Wolfgang Gruener, Beate Graeter, Tilmann Reinehr, Michael Weber, Achim Deplazes, Peter Möller, Peter Beck, Annika Barth, Thomas F E |
| description | Alveolar (AE) and cystic echinococcosis (CE) in humans are caused by the metacestode of the tapeworms Echinococcus multilocularis and Echinococcus granulosus sensu lato (s.l.). Immunohistochemistry with the monoclonal antibodies (mAb) Em2G11, specific for AE, and the mAb EmG3, specific for AE and CE, is an important pillar of the histological diagnosis of these two infections. Our aim was to further evaluate mAb EmG3 in a diagnostic setting and to analyze in detail the localization, distribution, and impact of small particles of Echinococcus multilocularis (spems) and small particles of Echinococcus granulosus s.l. (spegs) on lymph nodes.
We evaluated the mAb EmG3 in a cohort of formalin-fixed, paraffin embedded (FFPE) specimens of AE (n = 360) and CE (n = 178). These samples originated from 156 AE-patients and 77 CE-patients. mAb EmG3 showed a specific staining of the metacestode stadium of E. multilocularis and E. granulosus s.l. and had a higher sensitivity for spems than mAb Em2G11. Furthermore, we detected spegs in the surrounding host tissue and in almost all tested lymph nodes (39/41) of infected patients. 38/47 lymph nodes of AE showed a positive reaction for spems with mAb EmG3, whereas 29/47 tested positive when stained with mAb Em2G11. Spegs were detected in the germinal centers, co-located with CD23-positive follicular dendritic cells, and were present in the sinuses. Likewise, lymph nodes with spems and spegs in AE and CE were significantly enlarged in size in comparison to the control group.
mAb EmG3 is specific for AE and CE and is a valuable tool in the histological diagnosis of echinococcosis. Based on the observed staining patterns, we hypothesize that the interaction between parasite and host is not restricted to the main lesion since spegs are detected in lymph nodes. Moreover, in AE the number of spems-affected lymph nodes is higher than previously assumed. The enlargement of lymph nodes with spems and spegs points to an immunological interaction with the small immunogenic particles (spems and spegs) of Echinococcus spp. |
| doi_str_mv | 10.1371/journal.pntd.0008921 |
| format | Article |
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We evaluated the mAb EmG3 in a cohort of formalin-fixed, paraffin embedded (FFPE) specimens of AE (n = 360) and CE (n = 178). These samples originated from 156 AE-patients and 77 CE-patients. mAb EmG3 showed a specific staining of the metacestode stadium of E. multilocularis and E. granulosus s.l. and had a higher sensitivity for spems than mAb Em2G11. Furthermore, we detected spegs in the surrounding host tissue and in almost all tested lymph nodes (39/41) of infected patients. 38/47 lymph nodes of AE showed a positive reaction for spems with mAb EmG3, whereas 29/47 tested positive when stained with mAb Em2G11. Spegs were detected in the germinal centers, co-located with CD23-positive follicular dendritic cells, and were present in the sinuses. Likewise, lymph nodes with spems and spegs in AE and CE were significantly enlarged in size in comparison to the control group.
mAb EmG3 is specific for AE and CE and is a valuable tool in the histological diagnosis of echinococcosis. Based on the observed staining patterns, we hypothesize that the interaction between parasite and host is not restricted to the main lesion since spegs are detected in lymph nodes. Moreover, in AE the number of spems-affected lymph nodes is higher than previously assumed. 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This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 Grimm et al 2020 Grimm et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c624t-62e6974b1e3ad328474d5c10d03112b15dc601781612c08a93801714aa1d2e413</citedby><cites>FETCH-LOGICAL-c624t-62e6974b1e3ad328474d5c10d03112b15dc601781612c08a93801714aa1d2e413</cites><orcidid>0000-0003-3912-7749 ; 0000-0002-3379-6311 ; 0000-0002-5699-9031 ; 0000-0003-0073-3637 ; 0000-0003-3321-0920</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769273/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769273/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,2926,23864,27922,27923,53789,53791,79370,79371</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33370302$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Werneck, Guilherme L.</contributor><creatorcontrib>Grimm, Johannes</creatorcontrib><creatorcontrib>Nell, Juliane</creatorcontrib><creatorcontrib>Hillenbrand, Andreas</creatorcontrib><creatorcontrib>Henne-Bruns, Doris</creatorcontrib><creatorcontrib>Schmidberger, Julian</creatorcontrib><creatorcontrib>Kratzer, Wolfgang</creatorcontrib><creatorcontrib>Gruener, Beate</creatorcontrib><creatorcontrib>Graeter, Tilmann</creatorcontrib><creatorcontrib>Reinehr, Michael</creatorcontrib><creatorcontrib>Weber, Achim</creatorcontrib><creatorcontrib>Deplazes, Peter</creatorcontrib><creatorcontrib>Möller, Peter</creatorcontrib><creatorcontrib>Beck, Annika</creatorcontrib><creatorcontrib>Barth, Thomas F E</creatorcontrib><title>Immunohistological detection of small particles of Echinococcus multilocularis and Echinococcus granulosus in lymph nodes is associated with enlarged lymph nodes in alveolar and cystic echinococcosis</title><title>PLoS neglected tropical diseases</title><addtitle>PLoS Negl Trop Dis</addtitle><description>Alveolar (AE) and cystic echinococcosis (CE) in humans are caused by the metacestode of the tapeworms Echinococcus multilocularis and Echinococcus granulosus sensu lato (s.l.). Immunohistochemistry with the monoclonal antibodies (mAb) Em2G11, specific for AE, and the mAb EmG3, specific for AE and CE, is an important pillar of the histological diagnosis of these two infections. Our aim was to further evaluate mAb EmG3 in a diagnostic setting and to analyze in detail the localization, distribution, and impact of small particles of Echinococcus multilocularis (spems) and small particles of Echinococcus granulosus s.l. (spegs) on lymph nodes.
We evaluated the mAb EmG3 in a cohort of formalin-fixed, paraffin embedded (FFPE) specimens of AE (n = 360) and CE (n = 178). These samples originated from 156 AE-patients and 77 CE-patients. mAb EmG3 showed a specific staining of the metacestode stadium of E. multilocularis and E. granulosus s.l. and had a higher sensitivity for spems than mAb Em2G11. Furthermore, we detected spegs in the surrounding host tissue and in almost all tested lymph nodes (39/41) of infected patients. 38/47 lymph nodes of AE showed a positive reaction for spems with mAb EmG3, whereas 29/47 tested positive when stained with mAb Em2G11. Spegs were detected in the germinal centers, co-located with CD23-positive follicular dendritic cells, and were present in the sinuses. Likewise, lymph nodes with spems and spegs in AE and CE were significantly enlarged in size in comparison to the control group.
mAb EmG3 is specific for AE and CE and is a valuable tool in the histological diagnosis of echinococcosis. Based on the observed staining patterns, we hypothesize that the interaction between parasite and host is not restricted to the main lesion since spegs are detected in lymph nodes. Moreover, in AE the number of spems-affected lymph nodes is higher than previously assumed. The enlargement of lymph nodes with spems and spegs points to an immunological interaction with the small immunogenic particles (spems and spegs) of Echinococcus spp.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Alveoli</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antigens</subject><subject>Biology and Life Sciences</subject><subject>Bladder</subject><subject>Care and treatment</subject><subject>Child</subject><subject>Cysts</subject><subject>Diagnosis</subject><subject>Diagnostic Tests, Routine</subject><subject>Echinococcosis</subject><subject>Echinococcosis - diagnosis</subject><subject>Echinococcosis - parasitology</subject><subject>Echinococcosis - pathology</subject><subject>Echinococcosis, Hepatic - diagnosis</subject><subject>Echinococcosis, Hepatic - parasitology</subject><subject>Echinococcosis, Hepatic - pathology</subject><subject>Echinococcus granulosus - immunology</subject><subject>Echinococcus multilocularis</subject><subject>Echinococcus multilocularis - immunology</subject><subject>Ethics</subject><subject>Evaluation</subject><subject>Female</subject><subject>Glycoproteins</subject><subject>Growth patterns</subject><subject>Health aspects</subject><subject>Histology</subject><subject>Human tissues</subject><subject>Humans</subject><subject>Immunohistochemistry - methods</subject><subject>Lesions</subject><subject>Liver</subject><subject>Lymph</subject><subject>Lymph nodes</subject><subject>Lymph Nodes - parasitology</subject><subject>Lymph Nodes - pathology</subject><subject>Lymphadenopathy - parasitology</subject><subject>Lymphatic system</subject><subject>Male</subject><subject>Medical examination</subject><subject>Medicine and Health Sciences</subject><subject>Metastasis</subject><subject>Microscopes</subject><subject>Middle Aged</subject><subject>Monoclonal antibodies</subject><subject>Morphology</subject><subject>Parasites</subject><subject>Patients</subject><subject>Research and Analysis Methods</subject><subject>Tissue</subject><subject>Tissues</subject><subject>Tropical diseases</subject><subject>Ultrasonic imaging</subject><subject>Young 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Abstracts</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PLoS neglected tropical diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grimm, Johannes</au><au>Nell, Juliane</au><au>Hillenbrand, Andreas</au><au>Henne-Bruns, Doris</au><au>Schmidberger, Julian</au><au>Kratzer, Wolfgang</au><au>Gruener, Beate</au><au>Graeter, Tilmann</au><au>Reinehr, Michael</au><au>Weber, Achim</au><au>Deplazes, Peter</au><au>Möller, Peter</au><au>Beck, Annika</au><au>Barth, Thomas F E</au><au>Werneck, Guilherme L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunohistological detection of small particles of Echinococcus multilocularis and Echinococcus granulosus in lymph nodes is associated with enlarged lymph nodes in alveolar and cystic echinococcosis</atitle><jtitle>PLoS neglected tropical diseases</jtitle><addtitle>PLoS Negl Trop Dis</addtitle><date>2020-12-01</date><risdate>2020</risdate><volume>14</volume><issue>12</issue><spage>e0008921</spage><epage>e0008921</epage><pages>e0008921-e0008921</pages><issn>1935-2735</issn><issn>1935-2727</issn><eissn>1935-2735</eissn><abstract>Alveolar (AE) and cystic echinococcosis (CE) in humans are caused by the metacestode of the tapeworms Echinococcus multilocularis and Echinococcus granulosus sensu lato (s.l.). Immunohistochemistry with the monoclonal antibodies (mAb) Em2G11, specific for AE, and the mAb EmG3, specific for AE and CE, is an important pillar of the histological diagnosis of these two infections. Our aim was to further evaluate mAb EmG3 in a diagnostic setting and to analyze in detail the localization, distribution, and impact of small particles of Echinococcus multilocularis (spems) and small particles of Echinococcus granulosus s.l. (spegs) on lymph nodes.
We evaluated the mAb EmG3 in a cohort of formalin-fixed, paraffin embedded (FFPE) specimens of AE (n = 360) and CE (n = 178). These samples originated from 156 AE-patients and 77 CE-patients. mAb EmG3 showed a specific staining of the metacestode stadium of E. multilocularis and E. granulosus s.l. and had a higher sensitivity for spems than mAb Em2G11. Furthermore, we detected spegs in the surrounding host tissue and in almost all tested lymph nodes (39/41) of infected patients. 38/47 lymph nodes of AE showed a positive reaction for spems with mAb EmG3, whereas 29/47 tested positive when stained with mAb Em2G11. Spegs were detected in the germinal centers, co-located with CD23-positive follicular dendritic cells, and were present in the sinuses. Likewise, lymph nodes with spems and spegs in AE and CE were significantly enlarged in size in comparison to the control group.
mAb EmG3 is specific for AE and CE and is a valuable tool in the histological diagnosis of echinococcosis. Based on the observed staining patterns, we hypothesize that the interaction between parasite and host is not restricted to the main lesion since spegs are detected in lymph nodes. Moreover, in AE the number of spems-affected lymph nodes is higher than previously assumed. The enlargement of lymph nodes with spems and spegs points to an immunological interaction with the small immunogenic particles (spems and spegs) of Echinococcus spp.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>33370302</pmid><doi>10.1371/journal.pntd.0008921</doi><orcidid>https://orcid.org/0000-0003-3912-7749</orcidid><orcidid>https://orcid.org/0000-0002-3379-6311</orcidid><orcidid>https://orcid.org/0000-0002-5699-9031</orcidid><orcidid>https://orcid.org/0000-0003-0073-3637</orcidid><orcidid>https://orcid.org/0000-0003-3321-0920</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1935-2735 |
| ispartof | PLoS neglected tropical diseases, 2020-12, Vol.14 (12), p.e0008921-e0008921 |
| issn | 1935-2735 1935-2727 1935-2735 |
| language | eng |
| recordid | cdi_plos_journals_2479472787 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; PubMed Central Open Access; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Adolescent Adult Aged Aged, 80 and over Alveoli Animals Antibodies, Monoclonal - immunology Antigens Biology and Life Sciences Bladder Care and treatment Child Cysts Diagnosis Diagnostic Tests, Routine Echinococcosis Echinococcosis - diagnosis Echinococcosis - parasitology Echinococcosis - pathology Echinococcosis, Hepatic - diagnosis Echinococcosis, Hepatic - parasitology Echinococcosis, Hepatic - pathology Echinococcus granulosus - immunology Echinococcus multilocularis Echinococcus multilocularis - immunology Ethics Evaluation Female Glycoproteins Growth patterns Health aspects Histology Human tissues Humans Immunohistochemistry - methods Lesions Liver Lymph Lymph nodes Lymph Nodes - parasitology Lymph Nodes - pathology Lymphadenopathy - parasitology Lymphatic system Male Medical examination Medicine and Health Sciences Metastasis Microscopes Middle Aged Monoclonal antibodies Morphology Parasites Patients Research and Analysis Methods Tissue Tissues Tropical diseases Ultrasonic imaging Young Adult |
| title | Immunohistological detection of small particles of Echinococcus multilocularis and Echinococcus granulosus in lymph nodes is associated with enlarged lymph nodes in alveolar and cystic echinococcosis |
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