Rapid detection of SARS-CoV-2 with CRISPR-Cas12a

The recent outbreak of betacoronavirus Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), which is responsible for the Coronavirus Disease 2019 (COVID-19) global pandemic, has created great challenges in viral diagnosis. The existing methods for nucleic acid detection are of high sensitiv...

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Veröffentlicht in:	PLoS biology 2020-12, Vol.18 (12), p.e3000978-e3000978
Hauptverfasser:	Xiong, Dan, Dai, Wenjun, Gong, Jiaojiao, Li, Guande, Liu, Nansong, Wu, Wei, Pan, Jiaqiang, Chen, Chen, Jiao, Yingzhen, Deng, Huina, Ye, Junwei, Zhang, Xuanxuan, Huang, Huiling, Li, Qianyun, Xue, Liang, Zhang, Xiuming, Tang, Guanghui
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biology and life sciences Control Coronaviridae Coronaviruses COVID-19 Testing - methods CRISPR CRISPR-Cas Systems - genetics Deoxyribonucleic acid DNA DNA-directed RNA polymerase Engineering and Technology Epidemics Fluorescence Genomes Humans Medicine and health sciences Methods and Resources Middle East respiratory syndrome Molecular diagnostic techniques Mutation Nucleotides Pandemics Recombinase Reproducibility of Results Research and Analysis Methods Respiration Respiratory diseases Room temperature SARS-CoV-2 - genetics SARS-CoV-2 - isolation & purification Sensitivity and Specificity Severe acute respiratory syndrome Severe acute respiratory syndrome coronavirus 2 Substrates Transcription Viral diseases Viruses
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