Poria cocos polysaccharide induced Th1-type immune responses to ovalbumin in mice
In the present study, we evaluated adjuvant potential of Poria cocos polysaccharide (PCP) on the Th1-type immune responses of C57/BL6 mice against ovalbumin (OVA). We first determined the effect of PCP on maturation of murine bone marrow derived dendritic cells (BMDCs), PCP significantly upregulated...
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| creator | Dong, Xiaoxiao Li, Boye Yu, Boyang Chen, Tian Hu, Qin Peng, Bo Sheng, Wang |
| description | In the present study, we evaluated adjuvant potential of Poria cocos polysaccharide (PCP) on the Th1-type immune responses of C57/BL6 mice against ovalbumin (OVA). We first determined the effect of PCP on maturation of murine bone marrow derived dendritic cells (BMDCs), PCP significantly upregulated surface expression of MHCII, CD40, CD80, CD86 and enhanced production of IL-6 and IL-12p40. In addition, PCP affected receptor-mediated endocytosis, but not pinocytosis in BMDCs. Furthermore, OVA + PCP immunization induced specific cytotoxic CD8+ T cell killing of OVA (257-264) peptide pulsed cell. When mice were immunized subcutaneously in a week interval with OVA + PCP. Serum were collected for measuring OVA-specific antibody and splenocytes were harvested for analyzing CD69, IFN-γ ELISpot and cytokines production. The result indicated that OVA-specific IgG, IgG2a and IgG1 antibody levels in serum were significantly elevated by PCP compared with control. PCP increased OVA-specific IFN-γ-secreting CD8+, CD4+ T cells, promoted CD8+ T cell proliferation and up-regulated Th-1 type (IFN-γ, IL-2) cytokine production. In conclusion, data suggest that PCP enhanced cellular immune response and possess potential as a vaccine adjuvant for Th1 immune response. |
| doi_str_mv | 10.1371/journal.pone.0245207 |
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We first determined the effect of PCP on maturation of murine bone marrow derived dendritic cells (BMDCs), PCP significantly upregulated surface expression of MHCII, CD40, CD80, CD86 and enhanced production of IL-6 and IL-12p40. In addition, PCP affected receptor-mediated endocytosis, but not pinocytosis in BMDCs. Furthermore, OVA + PCP immunization induced specific cytotoxic CD8+ T cell killing of OVA (257-264) peptide pulsed cell. When mice were immunized subcutaneously in a week interval with OVA + PCP. Serum were collected for measuring OVA-specific antibody and splenocytes were harvested for analyzing CD69, IFN-γ ELISpot and cytokines production. The result indicated that OVA-specific IgG, IgG2a and IgG1 antibody levels in serum were significantly elevated by PCP compared with control. PCP increased OVA-specific IFN-γ-secreting CD8+, CD4+ T cells, promoted CD8+ T cell proliferation and up-regulated Th-1 type (IFN-γ, IL-2) cytokine production. In conclusion, data suggest that PCP enhanced cellular immune response and possess potential as a vaccine adjuvant for Th1 immune response.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0245207</identifier><identifier>PMID: 33411807</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aluminum ; Animals ; Antibodies ; Antibody Formation - immunology ; Antigens ; Biological response modifiers ; Biology and life sciences ; Bone marrow ; Bone Marrow Cells - immunology ; CD4 antigen ; CD4 lymphocytes ; CD40 antigen ; CD69 antigen ; CD8 antigen ; CD8-Positive T-Lymphocytes - immunology ; CD80 antigen ; CD86 antigen ; Cell proliferation ; Composition ; Cytokines ; Cytokines - immunology ; Cytotoxicity ; Dendritic cells ; Dendritic Cells - immunology ; Endocytosis ; Enzyme-linked immunosorbent assay ; Experiments ; Female ; Flow cytometry ; Fungal Polysaccharides - chemistry ; Fungal Polysaccharides - immunology ; Globular proteins ; Health aspects ; Immune response ; Immune response (cell-mediated) ; Immune system ; Immunity, Cellular - drug effects ; Immunization ; Immunoglobulin G ; Immunoglobulin G - immunology ; Interleukin 12 ; Interleukin 2 ; Interleukin 6 ; Laboratory animals ; Lymphocytes ; Lymphocytes T ; Medical research ; Medicine and health sciences ; Mice ; Molecular weight ; Ovalbumin ; Ovalbumin - immunology ; Penicillin ; Peptides ; Pharmacology, Experimental ; Pinocytosis ; Polysaccharides ; Poria cocos ; Research and Analysis Methods ; Splenocytes ; Th1 Cells - immunology ; Vaccines ; Wolfiporia - chemistry ; Wood-decaying fungi ; γ-Interferon</subject><ispartof>PloS one, 2021-01, Vol.16 (1), p.e0245207-e0245207</ispartof><rights>COPYRIGHT 2021 Public Library of Science</rights><rights>2021 Dong et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 Dong et al 2021 Dong et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-68ba986761ea6252374593240aac1433cd0218d8b8091c3a94ead63748059d263</citedby><cites>FETCH-LOGICAL-c692t-68ba986761ea6252374593240aac1433cd0218d8b8091c3a94ead63748059d263</cites><orcidid>0000-0003-1196-879X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7790389/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7790389/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33411807$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Wang, Tianwen</contributor><creatorcontrib>Dong, Xiaoxiao</creatorcontrib><creatorcontrib>Li, Boye</creatorcontrib><creatorcontrib>Yu, Boyang</creatorcontrib><creatorcontrib>Chen, Tian</creatorcontrib><creatorcontrib>Hu, Qin</creatorcontrib><creatorcontrib>Peng, Bo</creatorcontrib><creatorcontrib>Sheng, Wang</creatorcontrib><title>Poria cocos polysaccharide induced Th1-type immune responses to ovalbumin in mice</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>In the present study, we evaluated adjuvant potential of Poria cocos polysaccharide (PCP) on the Th1-type immune responses of C57/BL6 mice against ovalbumin (OVA). We first determined the effect of PCP on maturation of murine bone marrow derived dendritic cells (BMDCs), PCP significantly upregulated surface expression of MHCII, CD40, CD80, CD86 and enhanced production of IL-6 and IL-12p40. In addition, PCP affected receptor-mediated endocytosis, but not pinocytosis in BMDCs. Furthermore, OVA + PCP immunization induced specific cytotoxic CD8+ T cell killing of OVA (257-264) peptide pulsed cell. When mice were immunized subcutaneously in a week interval with OVA + PCP. Serum were collected for measuring OVA-specific antibody and splenocytes were harvested for analyzing CD69, IFN-γ ELISpot and cytokines production. The result indicated that OVA-specific IgG, IgG2a and IgG1 antibody levels in serum were significantly elevated by PCP compared with control. PCP increased OVA-specific IFN-γ-secreting CD8+, CD4+ T cells, promoted CD8+ T cell proliferation and up-regulated Th-1 type (IFN-γ, IL-2) cytokine production. In conclusion, data suggest that PCP enhanced cellular immune response and possess potential as a vaccine adjuvant for Th1 immune response.</description><subject>Aluminum</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibody Formation - immunology</subject><subject>Antigens</subject><subject>Biological response modifiers</subject><subject>Biology and life sciences</subject><subject>Bone marrow</subject><subject>Bone Marrow Cells - immunology</subject><subject>CD4 antigen</subject><subject>CD4 lymphocytes</subject><subject>CD40 antigen</subject><subject>CD69 antigen</subject><subject>CD8 antigen</subject><subject>CD8-Positive T-Lymphocytes - immunology</subject><subject>CD80 antigen</subject><subject>CD86 antigen</subject><subject>Cell proliferation</subject><subject>Composition</subject><subject>Cytokines</subject><subject>Cytokines - immunology</subject><subject>Cytotoxicity</subject><subject>Dendritic cells</subject><subject>Dendritic Cells - immunology</subject><subject>Endocytosis</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Experiments</subject><subject>Female</subject><subject>Flow cytometry</subject><subject>Fungal Polysaccharides - chemistry</subject><subject>Fungal Polysaccharides - immunology</subject><subject>Globular proteins</subject><subject>Health aspects</subject><subject>Immune response</subject><subject>Immune response (cell-mediated)</subject><subject>Immune system</subject><subject>Immunity, Cellular - drug effects</subject><subject>Immunization</subject><subject>Immunoglobulin G</subject><subject>Immunoglobulin G - immunology</subject><subject>Interleukin 12</subject><subject>Interleukin 2</subject><subject>Interleukin 6</subject><subject>Laboratory animals</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Medical research</subject><subject>Medicine and health sciences</subject><subject>Mice</subject><subject>Molecular weight</subject><subject>Ovalbumin</subject><subject>Ovalbumin - immunology</subject><subject>Penicillin</subject><subject>Peptides</subject><subject>Pharmacology, Experimental</subject><subject>Pinocytosis</subject><subject>Polysaccharides</subject><subject>Poria cocos</subject><subject>Research and Analysis Methods</subject><subject>Splenocytes</subject><subject>Th1 Cells - immunology</subject><subject>Vaccines</subject><subject>Wolfiporia - chemistry</subject><subject>Wood-decaying fungi</subject><subject>γ-Interferon</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkttu1DAQhiMEoqXwBggiISG42MWHxHFukKqKw0qVyqFwa02cya5XThzspGLfHi-bVhvUC-QLW-Nv_hmP_yR5TsmS8oK-27rRd2CXvetwSViWM1I8SE5pydlCMMIfHp1PkichbAnJuRTicXLCeUapJMVp8vWL8wZS7bQLae_sLoDWG_CmxtR09aixTq83dDHs-hho27HD1GOIRQOGdHCpuwFbja3pIp62RuPT5FEDNuCzaT9Lfnz8cH3xeXF59Wl1cX650KJkw0LICkopCkERBMsZL7I8tpsRAE0zznVNGJW1rCQpqeZQZgi1iJQkeVkzwc-Slwfd3rqgpmkExbJCMFrmvIjE6kDUDraq96YFv1MOjPobcH6twA9GW1QatahQ6gbqJitZVdJKs9gZA6m1EBi13k_VxqrFWmM3eLAz0flNZzZq7W5UUZSEyzIKvJkEvPs1YhhUa4JGa6FDNx76zkWRUR7RV_-g979uotYQH2C6xsW6ei-qzkVOJM2JkJFa3kPFVWP8rGidxsT4LOHtLCEyA_4e1jCGoFbfv_0_e_Vzzr4-YjcIdtgEZ8fBRCvNwewAau9C8NjcDZkStXf-7TTU3vlqcn5Me3H8QXdJt1bnfwAH8f1W</recordid><startdate>20210107</startdate><enddate>20210107</enddate><creator>Dong, Xiaoxiao</creator><creator>Li, Boye</creator><creator>Yu, Boyang</creator><creator>Chen, Tian</creator><creator>Hu, Qin</creator><creator>Peng, Bo</creator><creator>Sheng, Wang</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-1196-879X</orcidid></search><sort><creationdate>20210107</creationdate><title>Poria cocos polysaccharide induced Th1-type immune responses to ovalbumin in mice</title><author>Dong, Xiaoxiao ; Li, Boye ; Yu, Boyang ; Chen, Tian ; Hu, Qin ; Peng, Bo ; Sheng, Wang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-68ba986761ea6252374593240aac1433cd0218d8b8091c3a94ead63748059d263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Aluminum</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antibody Formation - immunology</topic><topic>Antigens</topic><topic>Biological response modifiers</topic><topic>Biology and life sciences</topic><topic>Bone marrow</topic><topic>Bone Marrow Cells - immunology</topic><topic>CD4 antigen</topic><topic>CD4 lymphocytes</topic><topic>CD40 antigen</topic><topic>CD69 antigen</topic><topic>CD8 antigen</topic><topic>CD8-Positive T-Lymphocytes - immunology</topic><topic>CD80 antigen</topic><topic>CD86 antigen</topic><topic>Cell proliferation</topic><topic>Composition</topic><topic>Cytokines</topic><topic>Cytokines - immunology</topic><topic>Cytotoxicity</topic><topic>Dendritic cells</topic><topic>Dendritic Cells - immunology</topic><topic>Endocytosis</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Experiments</topic><topic>Female</topic><topic>Flow cytometry</topic><topic>Fungal Polysaccharides - chemistry</topic><topic>Fungal Polysaccharides - immunology</topic><topic>Globular proteins</topic><topic>Health aspects</topic><topic>Immune response</topic><topic>Immune response (cell-mediated)</topic><topic>Immune system</topic><topic>Immunity, Cellular - drug effects</topic><topic>Immunization</topic><topic>Immunoglobulin G</topic><topic>Immunoglobulin G - immunology</topic><topic>Interleukin 12</topic><topic>Interleukin 2</topic><topic>Interleukin 6</topic><topic>Laboratory animals</topic><topic>Lymphocytes</topic><topic>Lymphocytes T</topic><topic>Medical research</topic><topic>Medicine and health sciences</topic><topic>Mice</topic><topic>Molecular weight</topic><topic>Ovalbumin</topic><topic>Ovalbumin - immunology</topic><topic>Penicillin</topic><topic>Peptides</topic><topic>Pharmacology, Experimental</topic><topic>Pinocytosis</topic><topic>Polysaccharides</topic><topic>Poria cocos</topic><topic>Research and Analysis Methods</topic><topic>Splenocytes</topic><topic>Th1 Cells - immunology</topic><topic>Vaccines</topic><topic>Wolfiporia - chemistry</topic><topic>Wood-decaying fungi</topic><topic>γ-Interferon</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dong, Xiaoxiao</creatorcontrib><creatorcontrib>Li, Boye</creatorcontrib><creatorcontrib>Yu, Boyang</creatorcontrib><creatorcontrib>Chen, Tian</creatorcontrib><creatorcontrib>Hu, Qin</creatorcontrib><creatorcontrib>Peng, Bo</creatorcontrib><creatorcontrib>Sheng, Wang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dong, Xiaoxiao</au><au>Li, Boye</au><au>Yu, Boyang</au><au>Chen, Tian</au><au>Hu, Qin</au><au>Peng, Bo</au><au>Sheng, Wang</au><au>Wang, Tianwen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Poria cocos polysaccharide induced Th1-type immune responses to ovalbumin in mice</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2021-01-07</date><risdate>2021</risdate><volume>16</volume><issue>1</issue><spage>e0245207</spage><epage>e0245207</epage><pages>e0245207-e0245207</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>In the present study, we evaluated adjuvant potential of Poria cocos polysaccharide (PCP) on the Th1-type immune responses of C57/BL6 mice against ovalbumin (OVA). We first determined the effect of PCP on maturation of murine bone marrow derived dendritic cells (BMDCs), PCP significantly upregulated surface expression of MHCII, CD40, CD80, CD86 and enhanced production of IL-6 and IL-12p40. In addition, PCP affected receptor-mediated endocytosis, but not pinocytosis in BMDCs. Furthermore, OVA + PCP immunization induced specific cytotoxic CD8+ T cell killing of OVA (257-264) peptide pulsed cell. When mice were immunized subcutaneously in a week interval with OVA + PCP. Serum were collected for measuring OVA-specific antibody and splenocytes were harvested for analyzing CD69, IFN-γ ELISpot and cytokines production. The result indicated that OVA-specific IgG, IgG2a and IgG1 antibody levels in serum were significantly elevated by PCP compared with control. PCP increased OVA-specific IFN-γ-secreting CD8+, CD4+ T cells, promoted CD8+ T cell proliferation and up-regulated Th-1 type (IFN-γ, IL-2) cytokine production. In conclusion, data suggest that PCP enhanced cellular immune response and possess potential as a vaccine adjuvant for Th1 immune response.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>33411807</pmid><doi>10.1371/journal.pone.0245207</doi><tpages>e0245207</tpages><orcidid>https://orcid.org/0000-0003-1196-879X</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2021-01, Vol.16 (1), p.e0245207-e0245207 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2476219537 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Aluminum Animals Antibodies Antibody Formation - immunology Antigens Biological response modifiers Biology and life sciences Bone marrow Bone Marrow Cells - immunology CD4 antigen CD4 lymphocytes CD40 antigen CD69 antigen CD8 antigen CD8-Positive T-Lymphocytes - immunology CD80 antigen CD86 antigen Cell proliferation Composition Cytokines Cytokines - immunology Cytotoxicity Dendritic cells Dendritic Cells - immunology Endocytosis Enzyme-linked immunosorbent assay Experiments Female Flow cytometry Fungal Polysaccharides - chemistry Fungal Polysaccharides - immunology Globular proteins Health aspects Immune response Immune response (cell-mediated) Immune system Immunity, Cellular - drug effects Immunization Immunoglobulin G Immunoglobulin G - immunology Interleukin 12 Interleukin 2 Interleukin 6 Laboratory animals Lymphocytes Lymphocytes T Medical research Medicine and health sciences Mice Molecular weight Ovalbumin Ovalbumin - immunology Penicillin Peptides Pharmacology, Experimental Pinocytosis Polysaccharides Poria cocos Research and Analysis Methods Splenocytes Th1 Cells - immunology Vaccines Wolfiporia - chemistry Wood-decaying fungi γ-Interferon |
| title | Poria cocos polysaccharide induced Th1-type immune responses to ovalbumin in mice |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T06%3A57%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Poria%20cocos%20polysaccharide%20induced%20Th1-type%20immune%20responses%20to%20ovalbumin%20in%20mice&rft.jtitle=PloS%20one&rft.au=Dong,%20Xiaoxiao&rft.date=2021-01-07&rft.volume=16&rft.issue=1&rft.spage=e0245207&rft.epage=e0245207&rft.pages=e0245207-e0245207&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0245207&rft_dat=%3Cgale_plos_%3EA650815068%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2476219537&rft_id=info:pmid/33411807&rft_galeid=A650815068&rft_doaj_id=oai_doaj_org_article_cec6be8cfadf492b91bc21ea2a8cc66e&rfr_iscdi=true |