Neuroprotection mediated by ST266 requires full complement of proteins secreted by amnion-derived multipotent progenitor cells
ST266 is the biological secretome of cultured Amnion-derived Multipotent Progenitor cells containing multiple growth factors and cytokines. While intranasally-administered ST266 improves the phenotype in experimental optic neuritis, specific ST266 components mediating these effects are not known. We...
Gespeichert in:
| Veröffentlicht in: | PloS one 2021-01, Vol.16 (1), p.e0243862-e0243862 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0243862 |
|---|---|
| container_issue | 1 |
| container_start_page | e0243862 |
| container_title | PloS one |
| container_volume | 16 |
| creator | Willett, Keirnan Khan, Reas S Dine, Kimberly Wessel, Howard Kirshner, Ziv Z Sauer, Jodie L Ellis, Ashley Brown, Larry R Shindler, Kenneth S |
| description | ST266 is the biological secretome of cultured Amnion-derived Multipotent Progenitor cells containing multiple growth factors and cytokines. While intranasally-administered ST266 improves the phenotype in experimental optic neuritis, specific ST266 components mediating these effects are not known. We compared the effects of ST266 with and without removal of large molecular weight proteins both in vitro and in the multiple sclerosis model experimental autoimmune encephalomyelitis (EAE) in C57BL/6J mice. Mice were treated daily with intranasal vehicle, ST266 or lower molecular weight fraction of ST266. Retinal ganglion cells were counted in isolated retinas, and optic nerves were assessed for inflammation and demyelination. ST266 treatment significantly improved retinal ganglion cell survival and reduced optic nerve demyelination in EAE mice. The lower molecular weight ST266 fraction significantly improved optic nerve demyelination, but only showed a trend towards improved retinal ganglion cell survival. ST266 fractions below 50kDa increased Schwann cell proliferation in vitro, but were less effective than non-fractionated ST266. Demyelination attenuation was partially associated with the lower molecular weight ST266 fraction, but removal of higher molecular weight biomolecules from ST266 diminishes its neuroprotective effects, suggesting at least some high molecular weight proteins play a role in ST266-mediated neuroprotection. |
| doi_str_mv | 10.1371/journal.pone.0243862 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2475829505</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A647638975</galeid><doaj_id>oai_doaj_org_article_ed87775114e3425c94711c78818923c0</doaj_id><sourcerecordid>A647638975</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-32fdbc47cd06888e06460f1d2d1b7209e932897226f9eae17ed3bc34763bd3303</originalsourceid><addsrcrecordid>eNqNk0tr3DAUhU1padJp_0FpDYXSLmaqhy3Zm0AIfQyEBpq0WyHL1zMKsuVIcmg2_e2VZ5wwLlkUL2yuv3Ou77FukrzGaIUpx5-u7eA6aVa97WCFSEYLRp4kx7ikZMkIok8Pno-SF95fI5RHiD1PjijNEEMlPU7-fIfB2d7ZACpo26Ut1FoGqNPqLr28IoylDm4G7cCnzWBMqmzbG2ihC6lt0p1Qdz71oBxMMtl20WlZg9O3sdIOJug-clES-Q10OliXKjDGv0yeNdJ4eDXdF8nPL5-vzr4tzy--rs9Oz5eKlSQsKWnqSmVc1YgVRQGIZQw1uCY1rjhBJcRBi5ITwpoSJGAONa0UzTijVU0poovk7d63N9aLKTovSMbzgpR5DGaRrPdEbeW16J1upbsTVmqxK1i3EdIFrQwIqAvOeY5xBjQjuSozjrHiRYGLklA1djuZug1VzFPFyZ00M9P5m05vxcbeCs4LTlkZDT5MBs7eDOCDaLUfA5Md2GH33QyTnO_Qd_-gj083URsZB9BdY2NfNZqKUzbGFNMbqdUjVLxqaLWK56zRsT4TfJwJIhPgd9jIwXuxvvzx_-zFrzn7_oDdgjRh660ZxhPq52C2B5Wz3jtoHkLGSIxrcp-GGNdETGsSZW8Of9CD6H4v6F8N_w4R</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2475829505</pqid></control><display><type>article</type><title>Neuroprotection mediated by ST266 requires full complement of proteins secreted by amnion-derived multipotent progenitor cells</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Public Library of Science (PLoS)</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Willett, Keirnan ; Khan, Reas S ; Dine, Kimberly ; Wessel, Howard ; Kirshner, Ziv Z ; Sauer, Jodie L ; Ellis, Ashley ; Brown, Larry R ; Shindler, Kenneth S</creator><contributor>Kihara, Alexandre Hiroaki</contributor><creatorcontrib>Willett, Keirnan ; Khan, Reas S ; Dine, Kimberly ; Wessel, Howard ; Kirshner, Ziv Z ; Sauer, Jodie L ; Ellis, Ashley ; Brown, Larry R ; Shindler, Kenneth S ; Kihara, Alexandre Hiroaki</creatorcontrib><description>ST266 is the biological secretome of cultured Amnion-derived Multipotent Progenitor cells containing multiple growth factors and cytokines. While intranasally-administered ST266 improves the phenotype in experimental optic neuritis, specific ST266 components mediating these effects are not known. We compared the effects of ST266 with and without removal of large molecular weight proteins both in vitro and in the multiple sclerosis model experimental autoimmune encephalomyelitis (EAE) in C57BL/6J mice. Mice were treated daily with intranasal vehicle, ST266 or lower molecular weight fraction of ST266. Retinal ganglion cells were counted in isolated retinas, and optic nerves were assessed for inflammation and demyelination. ST266 treatment significantly improved retinal ganglion cell survival and reduced optic nerve demyelination in EAE mice. The lower molecular weight ST266 fraction significantly improved optic nerve demyelination, but only showed a trend towards improved retinal ganglion cell survival. ST266 fractions below 50kDa increased Schwann cell proliferation in vitro, but were less effective than non-fractionated ST266. Demyelination attenuation was partially associated with the lower molecular weight ST266 fraction, but removal of higher molecular weight biomolecules from ST266 diminishes its neuroprotective effects, suggesting at least some high molecular weight proteins play a role in ST266-mediated neuroprotection.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0243862</identifier><identifier>PMID: 33406093</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Amnion ; Amnion - cytology ; Animals ; Attenuation ; Biological products ; Biology and Life Sciences ; Biomolecules ; Care and treatment ; Cell growth ; Cell Proliferation ; Cell survival ; Cells (biology) ; Cellular proteins ; Complications and side effects ; Cytokines ; Demyelinating Diseases - complications ; Demyelinating Diseases - pathology ; Demyelination ; Encephalomyelitis ; Encephalomyelitis, Autoimmune, Experimental - complications ; Encephalomyelitis, Autoimmune, Experimental - pathology ; Experimental allergic encephalomyelitis ; Experiments ; Female ; Gait ; Genetic aspects ; Growth factors ; Health aspects ; Manufacturers ; Medicine and Health Sciences ; Mice ; Mice, Inbred C57BL ; Molecular Weight ; Multiple sclerosis ; Multipotent Stem Cells - cytology ; Myelin-Oligodendrocyte Glycoprotein ; Nerves ; Neuritis ; Neurologic manifestations of general diseases ; Neuroprotection ; Optic nerve ; Optic Nerve - pathology ; Optic neuritis ; Optic Neuritis - complications ; Optic Neuritis - pathology ; Peptides ; Phenotypes ; Prevention ; Progenitor cells ; Proteins ; Research and Analysis Methods ; Retina ; Retinal ganglion cells ; Retinal Ganglion Cells - pathology ; Schwann Cells - pathology ; Secretome ; Stem cells ; Survival ; Tuberculosis</subject><ispartof>PloS one, 2021-01, Vol.16 (1), p.e0243862-e0243862</ispartof><rights>COPYRIGHT 2021 Public Library of Science</rights><rights>2021 Willett et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 Willett et al 2021 Willett et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-32fdbc47cd06888e06460f1d2d1b7209e932897226f9eae17ed3bc34763bd3303</citedby><cites>FETCH-LOGICAL-c692t-32fdbc47cd06888e06460f1d2d1b7209e932897226f9eae17ed3bc34763bd3303</cites><orcidid>0000-0001-5569-7261 ; 0000-0002-6570-4602 ; 0000-0003-2417-258X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787369/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787369/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53769,53771,79346,79347</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33406093$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Kihara, Alexandre Hiroaki</contributor><creatorcontrib>Willett, Keirnan</creatorcontrib><creatorcontrib>Khan, Reas S</creatorcontrib><creatorcontrib>Dine, Kimberly</creatorcontrib><creatorcontrib>Wessel, Howard</creatorcontrib><creatorcontrib>Kirshner, Ziv Z</creatorcontrib><creatorcontrib>Sauer, Jodie L</creatorcontrib><creatorcontrib>Ellis, Ashley</creatorcontrib><creatorcontrib>Brown, Larry R</creatorcontrib><creatorcontrib>Shindler, Kenneth S</creatorcontrib><title>Neuroprotection mediated by ST266 requires full complement of proteins secreted by amnion-derived multipotent progenitor cells</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>ST266 is the biological secretome of cultured Amnion-derived Multipotent Progenitor cells containing multiple growth factors and cytokines. While intranasally-administered ST266 improves the phenotype in experimental optic neuritis, specific ST266 components mediating these effects are not known. We compared the effects of ST266 with and without removal of large molecular weight proteins both in vitro and in the multiple sclerosis model experimental autoimmune encephalomyelitis (EAE) in C57BL/6J mice. Mice were treated daily with intranasal vehicle, ST266 or lower molecular weight fraction of ST266. Retinal ganglion cells were counted in isolated retinas, and optic nerves were assessed for inflammation and demyelination. ST266 treatment significantly improved retinal ganglion cell survival and reduced optic nerve demyelination in EAE mice. The lower molecular weight ST266 fraction significantly improved optic nerve demyelination, but only showed a trend towards improved retinal ganglion cell survival. ST266 fractions below 50kDa increased Schwann cell proliferation in vitro, but were less effective than non-fractionated ST266. Demyelination attenuation was partially associated with the lower molecular weight ST266 fraction, but removal of higher molecular weight biomolecules from ST266 diminishes its neuroprotective effects, suggesting at least some high molecular weight proteins play a role in ST266-mediated neuroprotection.</description><subject>Amnion</subject><subject>Amnion - cytology</subject><subject>Animals</subject><subject>Attenuation</subject><subject>Biological products</subject><subject>Biology and Life Sciences</subject><subject>Biomolecules</subject><subject>Care and treatment</subject><subject>Cell growth</subject><subject>Cell Proliferation</subject><subject>Cell survival</subject><subject>Cells (biology)</subject><subject>Cellular proteins</subject><subject>Complications and side effects</subject><subject>Cytokines</subject><subject>Demyelinating Diseases - complications</subject><subject>Demyelinating Diseases - pathology</subject><subject>Demyelination</subject><subject>Encephalomyelitis</subject><subject>Encephalomyelitis, Autoimmune, Experimental - complications</subject><subject>Encephalomyelitis, Autoimmune, Experimental - pathology</subject><subject>Experimental allergic encephalomyelitis</subject><subject>Experiments</subject><subject>Female</subject><subject>Gait</subject><subject>Genetic aspects</subject><subject>Growth factors</subject><subject>Health aspects</subject><subject>Manufacturers</subject><subject>Medicine and Health Sciences</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Molecular Weight</subject><subject>Multiple sclerosis</subject><subject>Multipotent Stem Cells - cytology</subject><subject>Myelin-Oligodendrocyte Glycoprotein</subject><subject>Nerves</subject><subject>Neuritis</subject><subject>Neurologic manifestations of general diseases</subject><subject>Neuroprotection</subject><subject>Optic nerve</subject><subject>Optic Nerve - pathology</subject><subject>Optic neuritis</subject><subject>Optic Neuritis - complications</subject><subject>Optic Neuritis - pathology</subject><subject>Peptides</subject><subject>Phenotypes</subject><subject>Prevention</subject><subject>Progenitor cells</subject><subject>Proteins</subject><subject>Research and Analysis Methods</subject><subject>Retina</subject><subject>Retinal ganglion cells</subject><subject>Retinal Ganglion Cells - pathology</subject><subject>Schwann Cells - pathology</subject><subject>Secretome</subject><subject>Stem cells</subject><subject>Survival</subject><subject>Tuberculosis</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk0tr3DAUhU1padJp_0FpDYXSLmaqhy3Zm0AIfQyEBpq0WyHL1zMKsuVIcmg2_e2VZ5wwLlkUL2yuv3Ou77FukrzGaIUpx5-u7eA6aVa97WCFSEYLRp4kx7ikZMkIok8Pno-SF95fI5RHiD1PjijNEEMlPU7-fIfB2d7ZACpo26Ut1FoGqNPqLr28IoylDm4G7cCnzWBMqmzbG2ihC6lt0p1Qdz71oBxMMtl20WlZg9O3sdIOJug-clES-Q10OliXKjDGv0yeNdJ4eDXdF8nPL5-vzr4tzy--rs9Oz5eKlSQsKWnqSmVc1YgVRQGIZQw1uCY1rjhBJcRBi5ITwpoSJGAONa0UzTijVU0poovk7d63N9aLKTovSMbzgpR5DGaRrPdEbeW16J1upbsTVmqxK1i3EdIFrQwIqAvOeY5xBjQjuSozjrHiRYGLklA1djuZug1VzFPFyZ00M9P5m05vxcbeCs4LTlkZDT5MBs7eDOCDaLUfA5Md2GH33QyTnO_Qd_-gj083URsZB9BdY2NfNZqKUzbGFNMbqdUjVLxqaLWK56zRsT4TfJwJIhPgd9jIwXuxvvzx_-zFrzn7_oDdgjRh660ZxhPq52C2B5Wz3jtoHkLGSIxrcp-GGNdETGsSZW8Of9CD6H4v6F8N_w4R</recordid><startdate>20210106</startdate><enddate>20210106</enddate><creator>Willett, Keirnan</creator><creator>Khan, Reas S</creator><creator>Dine, Kimberly</creator><creator>Wessel, Howard</creator><creator>Kirshner, Ziv Z</creator><creator>Sauer, Jodie L</creator><creator>Ellis, Ashley</creator><creator>Brown, Larry R</creator><creator>Shindler, Kenneth S</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-5569-7261</orcidid><orcidid>https://orcid.org/0000-0002-6570-4602</orcidid><orcidid>https://orcid.org/0000-0003-2417-258X</orcidid></search><sort><creationdate>20210106</creationdate><title>Neuroprotection mediated by ST266 requires full complement of proteins secreted by amnion-derived multipotent progenitor cells</title><author>Willett, Keirnan ; Khan, Reas S ; Dine, Kimberly ; Wessel, Howard ; Kirshner, Ziv Z ; Sauer, Jodie L ; Ellis, Ashley ; Brown, Larry R ; Shindler, Kenneth S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-32fdbc47cd06888e06460f1d2d1b7209e932897226f9eae17ed3bc34763bd3303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Amnion</topic><topic>Amnion - cytology</topic><topic>Animals</topic><topic>Attenuation</topic><topic>Biological products</topic><topic>Biology and Life Sciences</topic><topic>Biomolecules</topic><topic>Care and treatment</topic><topic>Cell growth</topic><topic>Cell Proliferation</topic><topic>Cell survival</topic><topic>Cells (biology)</topic><topic>Cellular proteins</topic><topic>Complications and side effects</topic><topic>Cytokines</topic><topic>Demyelinating Diseases - complications</topic><topic>Demyelinating Diseases - pathology</topic><topic>Demyelination</topic><topic>Encephalomyelitis</topic><topic>Encephalomyelitis, Autoimmune, Experimental - complications</topic><topic>Encephalomyelitis, Autoimmune, Experimental - pathology</topic><topic>Experimental allergic encephalomyelitis</topic><topic>Experiments</topic><topic>Female</topic><topic>Gait</topic><topic>Genetic aspects</topic><topic>Growth factors</topic><topic>Health aspects</topic><topic>Manufacturers</topic><topic>Medicine and Health Sciences</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Molecular Weight</topic><topic>Multiple sclerosis</topic><topic>Multipotent Stem Cells - cytology</topic><topic>Myelin-Oligodendrocyte Glycoprotein</topic><topic>Nerves</topic><topic>Neuritis</topic><topic>Neurologic manifestations of general diseases</topic><topic>Neuroprotection</topic><topic>Optic nerve</topic><topic>Optic Nerve - pathology</topic><topic>Optic neuritis</topic><topic>Optic Neuritis - complications</topic><topic>Optic Neuritis - pathology</topic><topic>Peptides</topic><topic>Phenotypes</topic><topic>Prevention</topic><topic>Progenitor cells</topic><topic>Proteins</topic><topic>Research and Analysis Methods</topic><topic>Retina</topic><topic>Retinal ganglion cells</topic><topic>Retinal Ganglion Cells - pathology</topic><topic>Schwann Cells - pathology</topic><topic>Secretome</topic><topic>Stem cells</topic><topic>Survival</topic><topic>Tuberculosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Willett, Keirnan</creatorcontrib><creatorcontrib>Khan, Reas S</creatorcontrib><creatorcontrib>Dine, Kimberly</creatorcontrib><creatorcontrib>Wessel, Howard</creatorcontrib><creatorcontrib>Kirshner, Ziv Z</creatorcontrib><creatorcontrib>Sauer, Jodie L</creatorcontrib><creatorcontrib>Ellis, Ashley</creatorcontrib><creatorcontrib>Brown, Larry R</creatorcontrib><creatorcontrib>Shindler, Kenneth S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Willett, Keirnan</au><au>Khan, Reas S</au><au>Dine, Kimberly</au><au>Wessel, Howard</au><au>Kirshner, Ziv Z</au><au>Sauer, Jodie L</au><au>Ellis, Ashley</au><au>Brown, Larry R</au><au>Shindler, Kenneth S</au><au>Kihara, Alexandre Hiroaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neuroprotection mediated by ST266 requires full complement of proteins secreted by amnion-derived multipotent progenitor cells</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2021-01-06</date><risdate>2021</risdate><volume>16</volume><issue>1</issue><spage>e0243862</spage><epage>e0243862</epage><pages>e0243862-e0243862</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>ST266 is the biological secretome of cultured Amnion-derived Multipotent Progenitor cells containing multiple growth factors and cytokines. While intranasally-administered ST266 improves the phenotype in experimental optic neuritis, specific ST266 components mediating these effects are not known. We compared the effects of ST266 with and without removal of large molecular weight proteins both in vitro and in the multiple sclerosis model experimental autoimmune encephalomyelitis (EAE) in C57BL/6J mice. Mice were treated daily with intranasal vehicle, ST266 or lower molecular weight fraction of ST266. Retinal ganglion cells were counted in isolated retinas, and optic nerves were assessed for inflammation and demyelination. ST266 treatment significantly improved retinal ganglion cell survival and reduced optic nerve demyelination in EAE mice. The lower molecular weight ST266 fraction significantly improved optic nerve demyelination, but only showed a trend towards improved retinal ganglion cell survival. ST266 fractions below 50kDa increased Schwann cell proliferation in vitro, but were less effective than non-fractionated ST266. Demyelination attenuation was partially associated with the lower molecular weight ST266 fraction, but removal of higher molecular weight biomolecules from ST266 diminishes its neuroprotective effects, suggesting at least some high molecular weight proteins play a role in ST266-mediated neuroprotection.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>33406093</pmid><doi>10.1371/journal.pone.0243862</doi><tpages>e0243862</tpages><orcidid>https://orcid.org/0000-0001-5569-7261</orcidid><orcidid>https://orcid.org/0000-0002-6570-4602</orcidid><orcidid>https://orcid.org/0000-0003-2417-258X</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2021-01, Vol.16 (1), p.e0243862-e0243862 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2475829505 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Amnion Amnion - cytology Animals Attenuation Biological products Biology and Life Sciences Biomolecules Care and treatment Cell growth Cell Proliferation Cell survival Cells (biology) Cellular proteins Complications and side effects Cytokines Demyelinating Diseases - complications Demyelinating Diseases - pathology Demyelination Encephalomyelitis Encephalomyelitis, Autoimmune, Experimental - complications Encephalomyelitis, Autoimmune, Experimental - pathology Experimental allergic encephalomyelitis Experiments Female Gait Genetic aspects Growth factors Health aspects Manufacturers Medicine and Health Sciences Mice Mice, Inbred C57BL Molecular Weight Multiple sclerosis Multipotent Stem Cells - cytology Myelin-Oligodendrocyte Glycoprotein Nerves Neuritis Neurologic manifestations of general diseases Neuroprotection Optic nerve Optic Nerve - pathology Optic neuritis Optic Neuritis - complications Optic Neuritis - pathology Peptides Phenotypes Prevention Progenitor cells Proteins Research and Analysis Methods Retina Retinal ganglion cells Retinal Ganglion Cells - pathology Schwann Cells - pathology Secretome Stem cells Survival Tuberculosis |
| title | Neuroprotection mediated by ST266 requires full complement of proteins secreted by amnion-derived multipotent progenitor cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T23%3A40%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Neuroprotection%20mediated%20by%20ST266%20requires%20full%20complement%20of%20proteins%20secreted%20by%20amnion-derived%20multipotent%20progenitor%20cells&rft.jtitle=PloS%20one&rft.au=Willett,%20Keirnan&rft.date=2021-01-06&rft.volume=16&rft.issue=1&rft.spage=e0243862&rft.epage=e0243862&rft.pages=e0243862-e0243862&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0243862&rft_dat=%3Cgale_plos_%3EA647638975%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2475829505&rft_id=info:pmid/33406093&rft_galeid=A647638975&rft_doaj_id=oai_doaj_org_article_ed87775114e3425c94711c78818923c0&rfr_iscdi=true |