Acquisition and decay of IgM and IgG responses to merozoite antigens after Plasmodium falciparum malaria in Ghanaian children
Developing a vaccine against Plasmodium falciparum malaria has been challenging, primarily due to high levels of antigen polymorphism and a complex parasite lifecycle. Immunization with the P. falciparum merozoite antigens PfMSRP5, PfSERA9, PfRAMA, PfCyRPA and PfRH5 has been shown to give rise to gr...
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| description | Developing a vaccine against Plasmodium falciparum malaria has been challenging, primarily due to high levels of antigen polymorphism and a complex parasite lifecycle. Immunization with the P. falciparum merozoite antigens PfMSRP5, PfSERA9, PfRAMA, PfCyRPA and PfRH5 has been shown to give rise to growth inhibitory and synergistic antisera. Therefore, these five merozoite proteins are considered to be promising candidates for a second-generation multivalent malaria vaccine. Nevertheless, little is known about IgG and IgM responses to these antigens in populations that are naturally exposed to P. falciparum. In this study, serum samples from clinically immune adults and malaria exposed children from Ghana were studied to compare levels of IgG and IgM specific for PfMSRP5, PfSERA9, PfRAMA, PfCyRPA and PfRH5. All five antigens were found to be specifically recognized by both IgM and IgG in serum from clinically immune adults and from children with malaria. Longitudinal analysis of the latter group showed an early, transient IgM response that was followed by IgG, which peaked 14 days after the initial diagnosis. IgG levels and parasitemia did not correlate, whereas parasitemia was weakly positively correlated with IgM levels. These findings show that IgG and IgM specific for merozoite antigens PfMSRP5, PfSERA9, PfRAMA, PfCyRPA and PfRH5 are high in children during P. falciparum malaria, but that the IgM induction and decline occurs earlier in infection than that of IgG. |
| doi_str_mv | 10.1371/journal.pone.0243943 |
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Immunization with the P. falciparum merozoite antigens PfMSRP5, PfSERA9, PfRAMA, PfCyRPA and PfRH5 has been shown to give rise to growth inhibitory and synergistic antisera. Therefore, these five merozoite proteins are considered to be promising candidates for a second-generation multivalent malaria vaccine. Nevertheless, little is known about IgG and IgM responses to these antigens in populations that are naturally exposed to P. falciparum. In this study, serum samples from clinically immune adults and malaria exposed children from Ghana were studied to compare levels of IgG and IgM specific for PfMSRP5, PfSERA9, PfRAMA, PfCyRPA and PfRH5. All five antigens were found to be specifically recognized by both IgM and IgG in serum from clinically immune adults and from children with malaria. Longitudinal analysis of the latter group showed an early, transient IgM response that was followed by IgG, which peaked 14 days after the initial diagnosis. IgG levels and parasitemia did not correlate, whereas parasitemia was weakly positively correlated with IgM levels. These findings show that IgG and IgM specific for merozoite antigens PfMSRP5, PfSERA9, PfRAMA, PfCyRPA and PfRH5 are high in children during P. falciparum malaria, but that the IgM induction and decline occurs earlier in infection than that of IgG.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0243943</identifier><identifier>PMID: 33332459</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adolescent ; Adult ; Adults ; Aged ; Antibodies ; Antibodies, Protozoan - immunology ; Antigens ; Antigens, Protozoan - immunology ; Antisera ; Biology and Life Sciences ; Child ; Child, Preschool ; Children ; Development and progression ; Erythrocytes ; Ethics ; Female ; Health aspects ; Hospitals ; Humans ; Immune response ; Immunization ; Immunoglobulin G ; Immunoglobulin G - immunology ; Immunoglobulin M ; Immunoglobulin M - immunology ; Immunology ; Infant ; Infections ; Life cycle analysis ; Malaria ; Malaria vaccine ; Malaria Vaccines - immunology ; Malaria, Falciparum - immunology ; Malaria, Falciparum - parasitology ; Male ; Medical research ; Medicine and Health Sciences ; Merozoites - immunology ; Merozoites - pathogenicity ; Microscopy ; Middle Aged ; Parasite antigens ; Parasitemia ; Parasites ; Parasitology ; Pediatric research ; Plasmids ; Plasmodium falciparum ; Plasmodium falciparum - immunology ; Plasmodium falciparum - pathogenicity ; Polymorphism ; Prevention ; Research and Analysis Methods ; Vaccines ; Vector-borne diseases ; Viruses ; West Nile virus ; Young Adult</subject><ispartof>PloS one, 2020-12, Vol.15 (12), p.e0243943-e0243943</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Walker et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 Walker et al 2020 Walker et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-540f282acf57b6e5427b1978ac552cc04927f108311b64498204a705df5a0beb3</citedby><cites>FETCH-LOGICAL-c758t-540f282acf57b6e5427b1978ac552cc04927f108311b64498204a705df5a0beb3</cites><orcidid>0000-0002-1140-5527 ; 0000-0001-6510-495X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7746192/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7746192/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53769,53771,79346,79347</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33332459$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Braga, Érika Martins</contributor><creatorcontrib>Walker, Melanie R</creatorcontrib><creatorcontrib>Knudsen, Anne S</creatorcontrib><creatorcontrib>Partey, Frederica D</creatorcontrib><creatorcontrib>Bassi, Maria R</creatorcontrib><creatorcontrib>Frank, Asger M</creatorcontrib><creatorcontrib>Castberg, Filip C</creatorcontrib><creatorcontrib>Sarbah, Edem W</creatorcontrib><creatorcontrib>Ofori, Michael F</creatorcontrib><creatorcontrib>Hviid, Lars</creatorcontrib><creatorcontrib>Barfod, Lea</creatorcontrib><title>Acquisition and decay of IgM and IgG responses to merozoite antigens after Plasmodium falciparum malaria in Ghanaian children</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Developing a vaccine against Plasmodium falciparum malaria has been challenging, primarily due to high levels of antigen polymorphism and a complex parasite lifecycle. Immunization with the P. falciparum merozoite antigens PfMSRP5, PfSERA9, PfRAMA, PfCyRPA and PfRH5 has been shown to give rise to growth inhibitory and synergistic antisera. Therefore, these five merozoite proteins are considered to be promising candidates for a second-generation multivalent malaria vaccine. Nevertheless, little is known about IgG and IgM responses to these antigens in populations that are naturally exposed to P. falciparum. In this study, serum samples from clinically immune adults and malaria exposed children from Ghana were studied to compare levels of IgG and IgM specific for PfMSRP5, PfSERA9, PfRAMA, PfCyRPA and PfRH5. All five antigens were found to be specifically recognized by both IgM and IgG in serum from clinically immune adults and from children with malaria. Longitudinal analysis of the latter group showed an early, transient IgM response that was followed by IgG, which peaked 14 days after the initial diagnosis. IgG levels and parasitemia did not correlate, whereas parasitemia was weakly positively correlated with IgM levels. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Walker, Melanie R</au><au>Knudsen, Anne S</au><au>Partey, Frederica D</au><au>Bassi, Maria R</au><au>Frank, Asger M</au><au>Castberg, Filip C</au><au>Sarbah, Edem W</au><au>Ofori, Michael F</au><au>Hviid, Lars</au><au>Barfod, Lea</au><au>Braga, Érika Martins</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Acquisition and decay of IgM and IgG responses to merozoite antigens after Plasmodium falciparum malaria in Ghanaian children</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2020-12-17</date><risdate>2020</risdate><volume>15</volume><issue>12</issue><spage>e0243943</spage><epage>e0243943</epage><pages>e0243943-e0243943</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Developing a vaccine against Plasmodium falciparum malaria has been challenging, primarily due to high levels of antigen polymorphism and a complex parasite lifecycle. Immunization with the P. falciparum merozoite antigens PfMSRP5, PfSERA9, PfRAMA, PfCyRPA and PfRH5 has been shown to give rise to growth inhibitory and synergistic antisera. Therefore, these five merozoite proteins are considered to be promising candidates for a second-generation multivalent malaria vaccine. Nevertheless, little is known about IgG and IgM responses to these antigens in populations that are naturally exposed to P. falciparum. In this study, serum samples from clinically immune adults and malaria exposed children from Ghana were studied to compare levels of IgG and IgM specific for PfMSRP5, PfSERA9, PfRAMA, PfCyRPA and PfRH5. All five antigens were found to be specifically recognized by both IgM and IgG in serum from clinically immune adults and from children with malaria. Longitudinal analysis of the latter group showed an early, transient IgM response that was followed by IgG, which peaked 14 days after the initial diagnosis. IgG levels and parasitemia did not correlate, whereas parasitemia was weakly positively correlated with IgM levels. These findings show that IgG and IgM specific for merozoite antigens PfMSRP5, PfSERA9, PfRAMA, PfCyRPA and PfRH5 are high in children during P. falciparum malaria, but that the IgM induction and decline occurs earlier in infection than that of IgG.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>33332459</pmid><doi>10.1371/journal.pone.0243943</doi><tpages>e0243943</tpages><orcidid>https://orcid.org/0000-0002-1140-5527</orcidid><orcidid>https://orcid.org/0000-0001-6510-495X</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2020-12, Vol.15 (12), p.e0243943-e0243943 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2470907538 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adolescent Adult Adults Aged Antibodies Antibodies, Protozoan - immunology Antigens Antigens, Protozoan - immunology Antisera Biology and Life Sciences Child Child, Preschool Children Development and progression Erythrocytes Ethics Female Health aspects Hospitals Humans Immune response Immunization Immunoglobulin G Immunoglobulin G - immunology Immunoglobulin M Immunoglobulin M - immunology Immunology Infant Infections Life cycle analysis Malaria Malaria vaccine Malaria Vaccines - immunology Malaria, Falciparum - immunology Malaria, Falciparum - parasitology Male Medical research Medicine and Health Sciences Merozoites - immunology Merozoites - pathogenicity Microscopy Middle Aged Parasite antigens Parasitemia Parasites Parasitology Pediatric research Plasmids Plasmodium falciparum Plasmodium falciparum - immunology Plasmodium falciparum - pathogenicity Polymorphism Prevention Research and Analysis Methods Vaccines Vector-borne diseases Viruses West Nile virus Young Adult |
| title | Acquisition and decay of IgM and IgG responses to merozoite antigens after Plasmodium falciparum malaria in Ghanaian children |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-24T12%3A23%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Acquisition%20and%20decay%20of%20IgM%20and%20IgG%20responses%20to%20merozoite%20antigens%20after%20Plasmodium%20falciparum%20malaria%20in%20Ghanaian%20children&rft.jtitle=PloS%20one&rft.au=Walker,%20Melanie%20R&rft.date=2020-12-17&rft.volume=15&rft.issue=12&rft.spage=e0243943&rft.epage=e0243943&rft.pages=e0243943-e0243943&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0243943&rft_dat=%3Cgale_plos_%3EA645382791%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2470907538&rft_id=info:pmid/33332459&rft_galeid=A645382791&rft_doaj_id=oai_doaj_org_article_1e5ad697108c41ac8910e77ff79f5a2e&rfr_iscdi=true |